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The map label for this gene is wcaJ [H]

Identifier: 91787754

GI number: 91787754

Start: 1937229

End: 1938662

Strand: Direct

Name: wcaJ [H]

Synonym: Bpro_1875

Alternate gene names: 91787754

Gene position: 1937229-1938662 (Clockwise)

Preceding gene: 91787753

Following gene: 91787755

Centisome position: 37.25

GC content: 58.37

Gene sequence:

>1434_bases
ATGGCTGAATTCAACCCGGTCAATTCGGCATCGGCATCAGCCTGGCTGGACCGCCGTGAGCCCCGATCCATGGGGTTGGG
CAGAAGCCATCTTTTCATCGCCATCGAAGCCGCGCTGGAGCCTCTGGTCCTGGTATTTTCGCTGTGGGCGTTGGCGTTTT
ACTTTGAAGACCAGGTCAGCCCGGCCTACCTGATCCTGTCGGTCATCGTGTTCTCGCTGTCTTTCCCGGGCACTTCCCAG
CTTCGCCTGCCGGTGGGCAAAGCCGTGCTCAATATCGCCATTCACTGGCTCTGGATCGCCGGGCTGTTGTTGCTGGCTGG
TGTGGCCACGGAATACCTTTACAGCTTGTCCGTGCCCGTGGTGGGCAACTGGTTATGGATTGCGCCGGTGGCCCAGATCG
CTGCACACCTCGGGCTTAGAGCTGCCGCACCCCAACTGCTGAAGTTGCAGGGCCCGCCCCGAAGCGCGGTCATTGTGGGC
ATGAACGAGCAGGGCGTGTCCCTGGCCCGGCATATCGCCGGGTCGGCCTACTCGGGTATCGAGGTGCTGGGATTTTTTGA
TGATCGCAGCGAAGAGCGCAGGGGCGCACAGGATGAGCAGGGGCCGATCGGCAAAATCGATGACTTGCCGGCGTATGTCA
AGCAGCACCGGGTCCAGTTTATTTACCTCTCCTTGCCGATGGCTTCGCGGCCACGCATTTTGCAGGTTCTTGACGGATTG
AAGGACACCACCGCCTCCATCTATTTTGTGCCCGACATGTTTGTGACGGACCTGATTCAGGGCCGAAGCGATTCGGTATG
CGGTGTCACGGTGATATCCGTTTGCGATACGCCGTTTCGTGGTGTCAATGGCGCATTGAAGCGAGTCAGCGACATTGTGT
TGTCATTGCTCATACTGCTCCTGATGTCTCCCCTCATGCTGGTGATTGCGCTGGCGGTGAAACTGGACTCGCCGGGGCCG
GTGATCTTCAAGCAGCGGCGCTACGGACTGGATGGCGAGCAGATCCTGGTCTATAAATTCCGATCCATGGCCGTGACCGA
AGACGGCAACACGATTCAGCAAGCGCAGAAAAATGACATGCGGGTCACCCGGCTGGGCGCCTTCCTGCGCAGAACCTCGC
TCGATGAGCTGCCGCAATTCATCAACGTCCTGCAGGGGCGCATGAGTATTGTCGGGCCCCGTCCCCATGCGGTTGCGCAC
AACGAAATGTACCGGACCCTGATCAAGGGCTACATGGTTCGCCACAAGGTCCGGTCCGGAATTACCGGTTGGGCCCAGGT
CAATGGGCAGCGCGGCGAAACGGACACCCTGGACAAGATGGAGGCCCGCATTGACTGCGATCTTGATTATTTGAGAAATT
GGTCGTTGCAGCTCGACCTGTTCATTATCTTGAAGACTGTGCGGCTTGTTTTCAAAGACAGTTCCGCATACTGA

Upstream 100 bases:

>100_bases
AGTGGGTTCACTGTCAAAAACCACATGCAGCGCATCTTTCAGAAATTGAATGTTTTCAATCGGACGCAGGCAGTTTCCAA
GGTAACCAGGGTGGCTCTCA

Downstream 100 bases:

>100_bases
AACGGATGGGATGCAATGCGTCAAAGCGTCAATGTGCCCAGTAGTGCAAAAGAGCTATTTCACAACCTACACATTCCTAT
GAAACTAACATCTTTACTCC

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MAEFNPVNSASASAWLDRREPRSMGLGRSHLFIAIEAALEPLVLVFSLWALAFYFEDQVSPAYLILSVIVFSLSFPGTSQ
LRLPVGKAVLNIAIHWLWIAGLLLLAGVATEYLYSLSVPVVGNWLWIAPVAQIAAHLGLRAAAPQLLKLQGPPRSAVIVG
MNEQGVSLARHIAGSAYSGIEVLGFFDDRSEERRGAQDEQGPIGKIDDLPAYVKQHRVQFIYLSLPMASRPRILQVLDGL
KDTTASIYFVPDMFVTDLIQGRSDSVCGVTVISVCDTPFRGVNGALKRVSDIVLSLLILLLMSPLMLVIALAVKLDSPGP
VIFKQRRYGLDGEQILVYKFRSMAVTEDGNTIQQAQKNDMRVTRLGAFLRRTSLDELPQFINVLQGRMSIVGPRPHAVAH
NEMYRTLIKGYMVRHKVRSGITGWAQVNGQRGETDTLDKMEARIDCDLDYLRNWSLQLDLFIILKTVRLVFKDSSAY

Sequences:

>Translated_477_residues
MAEFNPVNSASASAWLDRREPRSMGLGRSHLFIAIEAALEPLVLVFSLWALAFYFEDQVSPAYLILSVIVFSLSFPGTSQ
LRLPVGKAVLNIAIHWLWIAGLLLLAGVATEYLYSLSVPVVGNWLWIAPVAQIAAHLGLRAAAPQLLKLQGPPRSAVIVG
MNEQGVSLARHIAGSAYSGIEVLGFFDDRSEERRGAQDEQGPIGKIDDLPAYVKQHRVQFIYLSLPMASRPRILQVLDGL
KDTTASIYFVPDMFVTDLIQGRSDSVCGVTVISVCDTPFRGVNGALKRVSDIVLSLLILLLMSPLMLVIALAVKLDSPGP
VIFKQRRYGLDGEQILVYKFRSMAVTEDGNTIQQAQKNDMRVTRLGAFLRRTSLDELPQFINVLQGRMSIVGPRPHAVAH
NEMYRTLIKGYMVRHKVRSGITGWAQVNGQRGETDTLDKMEARIDCDLDYLRNWSLQLDLFIILKTVRLVFKDSSAY
>Mature_476_residues
AEFNPVNSASASAWLDRREPRSMGLGRSHLFIAIEAALEPLVLVFSLWALAFYFEDQVSPAYLILSVIVFSLSFPGTSQL
RLPVGKAVLNIAIHWLWIAGLLLLAGVATEYLYSLSVPVVGNWLWIAPVAQIAAHLGLRAAAPQLLKLQGPPRSAVIVGM
NEQGVSLARHIAGSAYSGIEVLGFFDDRSEERRGAQDEQGPIGKIDDLPAYVKQHRVQFIYLSLPMASRPRILQVLDGLK
DTTASIYFVPDMFVTDLIQGRSDSVCGVTVISVCDTPFRGVNGALKRVSDIVLSLLILLLMSPLMLVIALAVKLDSPGPV
IFKQRRYGLDGEQILVYKFRSMAVTEDGNTIQQAQKNDMRVTRLGAFLRRTSLDELPQFINVLQGRMSIVGPRPHAVAHN
EMYRTLIKGYMVRHKVRSGITGWAQVNGQRGETDTLDKMEARIDCDLDYLRNWSLQLDLFIILKTVRLVFKDSSAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=381, Percent_Identity=44.8818897637795, Blast_Score=326, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52919; Mature: 52788

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFNPVNSASASAWLDRREPRSMGLGRSHLFIAIEAALEPLVLVFSLWALAFYFEDQVS
CCCCCCCCCCCHHHHHHCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCC
PAYLILSVIVFSLSFPGTSQLRLPVGKAVLNIAIHWLWIAGLLLLAGVATEYLYSLSVPV
HHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VGNWLWIAPVAQIAAHLGLRAAAPQLLKLQGPPRSAVIVGMNEQGVSLARHIAGSAYSGI
HCCCHHHHHHHHHHHHHCHHHCCCHHEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCE
EVLGFFDDRSEERRGAQDEQGPIGKIDDLPAYVKQHRVQFIYLSLPMASRPRILQVLDGL
EEEEECCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCHHHHHHCCC
KDTTASIYFVPDMFVTDLIQGRSDSVCGVTVISVCDTPFRGVNGALKRVSDIVLSLLILL
CCCCEEEEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LMSPLMLVIALAVKLDSPGPVIFKQRRYGLDGEQILVYKFRSMAVTEDGNTIQQAQKNDM
HHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCEEEEEEEEHEEEECCCCHHHHHHHCCC
RVTRLGAFLRRTSLDELPQFINVLQGRMSIVGPRPHAVAHNEMYRTLIKGYMVRHKVRSG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ITGWAQVNGQRGETDTLDKMEARIDCDLDYLRNWSLQLDLFIILKTVRLVFKDSSAY
CCCCEEECCCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEFNPVNSASASAWLDRREPRSMGLGRSHLFIAIEAALEPLVLVFSLWALAFYFEDQVS
CCCCCCCCCCHHHHHHCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCC
PAYLILSVIVFSLSFPGTSQLRLPVGKAVLNIAIHWLWIAGLLLLAGVATEYLYSLSVPV
HHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VGNWLWIAPVAQIAAHLGLRAAAPQLLKLQGPPRSAVIVGMNEQGVSLARHIAGSAYSGI
HCCCHHHHHHHHHHHHHCHHHCCCHHEEECCCCCCEEEEECCCCHHHHHHHHCCCCCCCE
EVLGFFDDRSEERRGAQDEQGPIGKIDDLPAYVKQHRVQFIYLSLPMASRPRILQVLDGL
EEEEECCCCCHHHCCCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCCCCHHHHHHCCC
KDTTASIYFVPDMFVTDLIQGRSDSVCGVTVISVCDTPFRGVNGALKRVSDIVLSLLILL
CCCCEEEEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
LMSPLMLVIALAVKLDSPGPVIFKQRRYGLDGEQILVYKFRSMAVTEDGNTIQQAQKNDM
HHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCEEEEEEEEHEEEECCCCHHHHHHHCCC
RVTRLGAFLRRTSLDELPQFINVLQGRMSIVGPRPHAVAHNEMYRTLIKGYMVRHKVRSG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ITGWAQVNGQRGETDTLDKMEARIDCDLDYLRNWSLQLDLFIILKTVRLVFKDSSAY
CCCCEEECCCCCCCCHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]