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The map label for this gene is pcaB [H]

Identifier: 91787734

GI number: 91787734

Start: 1916457

End: 1917716

Strand: Direct

Name: pcaB [H]

Synonym: Bpro_1855

Alternate gene names: 91787734

Gene position: 1916457-1917716 (Clockwise)

Preceding gene: 91787733

Following gene: 91787735

Centisome position: 36.85

GC content: 67.06

Gene sequence:

>1260_bases
ATGAGCAGCATTTTTGAAGGTTTCCTGTCCACGTCCGAAATCCTGGAGGCGTTCAGTGAGCGCAATTTTCTGGATGCCAT
GCTGCGCTTTGAGGGCTCGCTGGCGCGCGCGCAGGCCAGCGTCGGGCTGATTCCCGAGGCCGTGGCCCAGTCCATCAGCG
GGACCTGCAAGGTCGAGCTGTTTGATGTGGCCAAGATCGTGCGCGAAAGCGGGCGCGCCGGCAGCATCGCGATTCCGCTG
GTCAAGAGCCTGAAGGAAACGGTGGGCCTCTTCAACAAGGATGCCGCCGGTTTTGTCCACTTTGGCTCTACCTCCCAGGA
CGTGATCGATACCGCGCTGGCGCTGGTGACCCGCAATGCGCTGAACCTGATCGAAGCCGATGTCCACAAGGCGGTGGCGG
CGTTGCTGGTCCTGGCCGGGCGCCATGCCGCCGATCCTGTGCTGGCGCGCACACTGATGCAGCCGGCGTCGGTCACCAGT
TTTGGCCTGAAATGCGCCGGATGGGCCGCACCGCTGGTACGCAGCCTGCAGCGCCTGCAGGCGACAGCCGGCAATGCCCT
GAGCGTGCAGCTGGGTGGCGCGGTGGGCACGCTCGCCCAGATGCAGAAAGCAGGAAAGAATCTGGGGCCGCAGGTCATCG
CGCACATGGCGAGCGAGCTGAAACTGAGGGCGCCTTCCTGCGCATGGCATACGCAGCGCGACGAGTGGGTCGCGCTCGGT
TGCGAGTTGGGGCTGCTGGTGGGCAGTCTTGGCAAGATCGCCAAGGACATTGCCCTGATGGGCCAGTATGAAGTGGGCGA
GGTGGCCGAGCCGACCGAGCCGGGGCGCGGGGGGTCGTCGGCCATGCCGCACAAGCGCAATCCGGTTGCCTGCATGGTGG
CGCTGGCTGCGGCGCAGCGGGCGCCGCAACGGGTGGCCGCGTTGCTGGCCGCCATGCCGCAGGAGCACGAGCGAGCCCTT
GGCAATTGGCAGGCCGAGCTGGCCGAATGGCCGGGCCTGCTGATGTCGGCGCACGGCTCCGCCCGTGCGATGGCCCAGGC
GCTGCCGGGCCTGCAGGTGGATACGCAGCGCATGCGGGCCAACCTGGACGCCTTGCGGGCGGTGCTGCCCGCCGAGGCTG
CAGACGAGTGGTTCAGTCCCGGCCTGGCCCGGCATGCGGCGGAGCTGACGCAGGCCCAGGTGCTGGCCCACACCCAGGCG
CTGGCTGCGCTGAAGCCCCCGAAATCACCAGATACCAACACAATGAAAGTGATGCCATGA

Upstream 100 bases:

>100_bases
CTGGGCGAGGACGTCTTGCGGCGTTCCGTGCCCTTGCCCAACCAGATCCGCCTGAAGCGTTGAAGGCCTGTTAATTCACT
TTTATGACCCCTGACATCGC

Downstream 100 bases:

>100_bases
GCACGAGTGACTCCATGAATGATTACGACAAGGGCATGGTCAATCGCCGCAGGGTGCTGGGCGATGCCTGGGTAGACAAA
TCCCTTGCCAACAGCAATGC

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSSIFEGFLSTSEILEAFSERNFLDAMLRFEGSLARAQASVGLIPEAVAQSISGTCKVELFDVAKIVRESGRAGSIAIPL
VKSLKETVGLFNKDAAGFVHFGSTSQDVIDTALALVTRNALNLIEADVHKAVAALLVLAGRHAADPVLARTLMQPASVTS
FGLKCAGWAAPLVRSLQRLQATAGNALSVQLGGAVGTLAQMQKAGKNLGPQVIAHMASELKLRAPSCAWHTQRDEWVALG
CELGLLVGSLGKIAKDIALMGQYEVGEVAEPTEPGRGGSSAMPHKRNPVACMVALAAAQRAPQRVAALLAAMPQEHERAL
GNWQAELAEWPGLLMSAHGSARAMAQALPGLQVDTQRMRANLDALRAVLPAEAADEWFSPGLARHAAELTQAQVLAHTQA
LAALKPPKSPDTNTMKVMP

Sequences:

>Translated_419_residues
MSSIFEGFLSTSEILEAFSERNFLDAMLRFEGSLARAQASVGLIPEAVAQSISGTCKVELFDVAKIVRESGRAGSIAIPL
VKSLKETVGLFNKDAAGFVHFGSTSQDVIDTALALVTRNALNLIEADVHKAVAALLVLAGRHAADPVLARTLMQPASVTS
FGLKCAGWAAPLVRSLQRLQATAGNALSVQLGGAVGTLAQMQKAGKNLGPQVIAHMASELKLRAPSCAWHTQRDEWVALG
CELGLLVGSLGKIAKDIALMGQYEVGEVAEPTEPGRGGSSAMPHKRNPVACMVALAAAQRAPQRVAALLAAMPQEHERAL
GNWQAELAEWPGLLMSAHGSARAMAQALPGLQVDTQRMRANLDALRAVLPAEAADEWFSPGLARHAAELTQAQVLAHTQA
LAALKPPKSPDTNTMKVMP
>Mature_418_residues
SSIFEGFLSTSEILEAFSERNFLDAMLRFEGSLARAQASVGLIPEAVAQSISGTCKVELFDVAKIVRESGRAGSIAIPLV
KSLKETVGLFNKDAAGFVHFGSTSQDVIDTALALVTRNALNLIEADVHKAVAALLVLAGRHAADPVLARTLMQPASVTSF
GLKCAGWAAPLVRSLQRLQATAGNALSVQLGGAVGTLAQMQKAGKNLGPQVIAHMASELKLRAPSCAWHTQRDEWVALGC
ELGLLVGSLGKIAKDIALMGQYEVGEVAEPTEPGRGGSSAMPHKRNPVACMVALAAAQRAPQRVAALLAAMPQEHERALG
NWQAELAEWPGLLMSAHGSARAMAQALPGLQVDTQRMRANLDALRAVLPAEAADEWFSPGLARHAAELTQAQVLAHTQAL
AALKPPKSPDTNTMKVMP

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=282, Percent_Identity=28.3687943262411, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4557269, Length=282, Percent_Identity=28.3687943262411, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=290, Percent_Identity=25.5172413793103, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI32564234, Length=279, Percent_Identity=25.4480286738351, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323391, Length=290, Percent_Identity=25.5172413793103, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24647570, Length=306, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 44094; Mature: 43963

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIFEGFLSTSEILEAFSERNFLDAMLRFEGSLARAQASVGLIPEAVAQSISGTCKVEL
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCEEEHH
FDVAKIVRESGRAGSIAIPLVKSLKETVGLFNKDAAGFVHFGSTSQDVIDTALALVTRNA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LNLIEADVHKAVAALLVLAGRHAADPVLARTLMQPASVTSFGLKCAGWAAPLVRSLQRLQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHH
ATAGNALSVQLGGAVGTLAQMQKAGKNLGPQVIAHMASELKLRAPSCAWHTQRDEWVALG
HHCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEH
CELGLLVGSLGKIAKDIALMGQYEVGEVAEPTEPGRGGSSAMPHKRNPVACMVALAAAQR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
APQRVAALLAAMPQEHERALGNWQAELAEWPGLLMSAHGSARAMAQALPGLQVDTQRMRA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHH
NLDALRAVLPAEAADEWFSPGLARHAAELTQAQVLAHTQALAALKPPKSPDTNTMKVMP
HHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCC
>Mature Secondary Structure 
SSIFEGFLSTSEILEAFSERNFLDAMLRFEGSLARAQASVGLIPEAVAQSISGTCKVEL
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHCCCCEEEHH
FDVAKIVRESGRAGSIAIPLVKSLKETVGLFNKDAAGFVHFGSTSQDVIDTALALVTRNA
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
LNLIEADVHKAVAALLVLAGRHAADPVLARTLMQPASVTSFGLKCAGWAAPLVRSLQRLQ
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHH
ATAGNALSVQLGGAVGTLAQMQKAGKNLGPQVIAHMASELKLRAPSCAWHTQRDEWVALG
HHCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEH
CELGLLVGSLGKIAKDIALMGQYEVGEVAEPTEPGRGGSSAMPHKRNPVACMVALAAAQR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
APQRVAALLAAMPQEHERALGNWQAELAEWPGLLMSAHGSARAMAQALPGLQVDTQRMRA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHH
NLDALRAVLPAEAADEWFSPGLARHAAELTQAQVLAHTQALAALKPPKSPDTNTMKVMP
HHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1390752 [H]