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The map label for this gene is braC [H]

Identifier: 91787727

GI number: 91787727

Start: 1909747

End: 1910916

Strand: Direct

Name: braC [H]

Synonym: Bpro_1848

Alternate gene names: 91787727

Gene position: 1909747-1910916 (Clockwise)

Preceding gene: 91787726

Following gene: 91787728

Centisome position: 36.72

GC content: 58.97

Gene sequence:

>1170_bases
ATGCAGAAACGATTTGCTCTTAAAGTGATAGCTGCTTGCGCAATAACCATGGCGGCTACGGCCGGATTTGCACAAGACGT
GATCAAGATTGCCAACATTGTCGAGCTGTCCGGTGGTGGTGCCACGGCCGGCACCAACTTCAAGAACGGCGTGGAACTGG
CCGTCAAGGAAATCAATGCCGCGGGCGGTATTCTCGGCAAAAAAATCCAGACCACCACCACCGACACCCAGAGCAACCCC
GGCGTGGCCAAGGGCCTGACGCAAAAGGCTATTGACGACAACGTCTTCGCCATCTTCGGTCCGGTGTTTTCCGGCTCCAT
CATGGTCAGCATGGCCGAGTCGCGCCGTGCCGAGGTGCCCAATTTCACGGGCGGCGAGGCGGCCAGCATCACCGAGCAGG
GCAACCCCTATGTGTTCCGGACCAGCTTCACACAGGCGACGGCCATGCCCAAGGTCGCCCGCTATATCAGCGATCAGGCC
AAACTCAAAAGCATCGCCATCATTTATGTGAACAACGATTTCGGCAAGGGTGGCCTTGACTCGATCAAGAAGGCGCTGGC
CAATTCGTCAACCAAGGTTGCCACCGAAATTTCCACCGAGCCCGGCCAGATCGACTTTTCGGCTGCCGTGCTGCGTGCCA
AGCAGAGCAATGCGGAAGGCGTGTTCGCCTACTCCAATGAAGAAGAGTCGGCGCGGCTGCTGCGCGAGTTGCGCAAGCAG
GGCTGGAACAAACCCGTCATTGGCGAAACCACGCTCACCGGCCAGAAGGTCATTGAGCTGGCGGGTGAGGCTGCCAACGG
CGCCGTGGCCCATGTGGGCCTGACGGTTGATGCGCCGCAGCCAGCCATTCGCGCCTTCCGTGCCAAGTTTGAGAAGGAAT
ACAAGTATGTGTCCGACCACAACGGCATGAAAGGCTACTCCGGCATCTACGTGCTGAAGTCCGCGATCGAGAAGGTCGGC
AAGCTCGACCGCAAGGCAGTGGCGGCTGCCCTGCATACCCTGAAGGTGAAAGCGGCGGATCAACCCGGCGTGCTGATGGA
TGTCTCGTTTGACAGCAAGGGCGACCTGGACCGCGAGAGTTTCATGATTGAAGTGAAGAACGGCAAGCAGGAAGTGGTTG
CCATCTTGCCACCGCTGGGTGCTGCGCCCGCTGCCGCACCAAAGAAATAA

Upstream 100 bases:

>100_bases
GTCGGGGAATCACGACTCCCGGCCCATTGATTCCATGTTGATTCCATATTGATCCCACATTGATTTTTTTACTGACTTTC
AACCCTTAGTGGAGACAACC

Downstream 100 bases:

>100_bases
GCCACCGGCCTCCGGCATCAACGCTTGACGTGACGCGCCGGTACGGGTGGCCCCGGGCCGCCGGTACCGGCAGCATGAAA
CCCTCCAGCAAGTACCTTCT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MQKRFALKVIAACAITMAATAGFAQDVIKIANIVELSGGGATAGTNFKNGVELAVKEINAAGGILGKKIQTTTTDTQSNP
GVAKGLTQKAIDDNVFAIFGPVFSGSIMVSMAESRRAEVPNFTGGEAASITEQGNPYVFRTSFTQATAMPKVARYISDQA
KLKSIAIIYVNNDFGKGGLDSIKKALANSSTKVATEISTEPGQIDFSAAVLRAKQSNAEGVFAYSNEEESARLLRELRKQ
GWNKPVIGETTLTGQKVIELAGEAANGAVAHVGLTVDAPQPAIRAFRAKFEKEYKYVSDHNGMKGYSGIYVLKSAIEKVG
KLDRKAVAAALHTLKVKAADQPGVLMDVSFDSKGDLDRESFMIEVKNGKQEVVAILPPLGAAPAAAPKK

Sequences:

>Translated_389_residues
MQKRFALKVIAACAITMAATAGFAQDVIKIANIVELSGGGATAGTNFKNGVELAVKEINAAGGILGKKIQTTTTDTQSNP
GVAKGLTQKAIDDNVFAIFGPVFSGSIMVSMAESRRAEVPNFTGGEAASITEQGNPYVFRTSFTQATAMPKVARYISDQA
KLKSIAIIYVNNDFGKGGLDSIKKALANSSTKVATEISTEPGQIDFSAAVLRAKQSNAEGVFAYSNEEESARLLRELRKQ
GWNKPVIGETTLTGQKVIELAGEAANGAVAHVGLTVDAPQPAIRAFRAKFEKEYKYVSDHNGMKGYSGIYVLKSAIEKVG
KLDRKAVAAALHTLKVKAADQPGVLMDVSFDSKGDLDRESFMIEVKNGKQEVVAILPPLGAAPAAAPKK
>Mature_389_residues
MQKRFALKVIAACAITMAATAGFAQDVIKIANIVELSGGGATAGTNFKNGVELAVKEINAAGGILGKKIQTTTTDTQSNP
GVAKGLTQKAIDDNVFAIFGPVFSGSIMVSMAESRRAEVPNFTGGEAASITEQGNPYVFRTSFTQATAMPKVARYISDQA
KLKSIAIIYVNNDFGKGGLDSIKKALANSSTKVATEISTEPGQIDFSAAVLRAKQSNAEGVFAYSNEEESARLLRELRKQ
GWNKPVIGETTLTGQKVIELAGEAANGAVAHVGLTVDAPQPAIRAFRAKFEKEYKYVSDHNGMKGYSGIYVLKSAIEKVG
KLDRKAVAAALHTLKVKAADQPGVLMDVSFDSKGDLDRESFMIEVKNGKQEVVAILPPLGAAPAAAPKK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=353, Percent_Identity=26.9121813031161, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994941, Length=352, Percent_Identity=26.1363636363636, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41059; Mature: 41059

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKRFALKVIAACAITMAATAGFAQDVIKIANIVELSGGGATAGTNFKNGVELAVKEINA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHH
AGGILGKKIQTTTTDTQSNPGVAKGLTQKAIDDNVFAIFGPVFSGSIMVSMAESRRAEVP
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHCCEEEEEEHHHHHCCCC
NFTGGEAASITEQGNPYVFRTSFTQATAMPKVARYISDQAKLKSIAIIYVNNDFGKGGLD
CCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCHH
SIKKALANSSTKVATEISTEPGQIDFSAAVLRAKQSNAEGVFAYSNEEESARLLRELRKQ
HHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHC
GWNKPVIGETTLTGQKVIELAGEAANGAVAHVGLTVDAPQPAIRAFRAKFEKEYKYVSDH
CCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
NGMKGYSGIYVLKSAIEKVGKLDRKAVAAALHTLKVKAADQPGVLMDVSFDSKGDLDRES
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCC
FMIEVKNGKQEVVAILPPLGAAPAAAPKK
EEEEECCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQKRFALKVIAACAITMAATAGFAQDVIKIANIVELSGGGATAGTNFKNGVELAVKEINA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHHHH
AGGILGKKIQTTTTDTQSNPGVAKGLTQKAIDDNVFAIFGPVFSGSIMVSMAESRRAEVP
CCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHCCEEEEEEHHHHHCCCC
NFTGGEAASITEQGNPYVFRTSFTQATAMPKVARYISDQAKLKSIAIIYVNNDFGKGGLD
CCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCHH
SIKKALANSSTKVATEISTEPGQIDFSAAVLRAKQSNAEGVFAYSNEEESARLLRELRKQ
HHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHC
GWNKPVIGETTLTGQKVIELAGEAANGAVAHVGLTVDAPQPAIRAFRAKFEKEYKYVSDH
CCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
NGMKGYSGIYVLKSAIEKVGKLDRKAVAAALHTLKVKAADQPGVLMDVSFDSKGDLDRES
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCC
FMIEVKNGKQEVVAILPPLGAAPAAAPKK
EEEEECCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]