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The map label for this gene is boxA [H]

Identifier: 91787512

GI number: 91787512

Start: 1684078

End: 1685358

Strand: Direct

Name: boxA [H]

Synonym: Bpro_1630

Alternate gene names: 91787512

Gene position: 1684078-1685358 (Clockwise)

Preceding gene: 91787511

Following gene: 91787513

Centisome position: 32.38

GC content: 61.51

Gene sequence:

>1281_bases
ATGGATATGACCGAAATACACGTTATCAAGCAGCACCTGATTGATCCCGAAATCTGCATCCGCTGCAACACCTGCGAAGC
CATCTGCCCGGTTCAGGCCATCACGCACGATTCACGCAACTACGTGGTCGATGCCGAAAAATGCAATCTCTGCATGGCCT
GCATATCCCCGTGCCCGACCGGCTCGATTGACAACTGGCGCACCATGCCGCGCATCAAGGCCTACAGCCTCACCGAGCAA
CTGGGCTGGGACGAGTTGCCCCCCGAACTCACCCCGGAAGAACTTGCGGCCGAGGGCGTGGTCCCCGGTGCGATGCCCGA
GATCGAACCGGTGGCGCCTTCACTGCCGGCTGCGGTCAGTGGCGAGACGGCCTTCAACAGTGCGCAGTACGGCGCCACAA
TGCCGCCCTGGTCGGCGGCGCACGGCTACACCAACCTGTACGGCGCCAAGGCGGCTGAAAAATTCATCACGGCCACGGTG
GTTGGCAATGTGCGCGTCACCGAAGTAGGCAAGGAGTACGACACCCACCACATCGTGCTCGACTTTGGCAGCATGCCCTT
CCCGGTGCTTGAAGGCCAGTCGATCGGCATTTTGCCGCCGGGAACCGACGAAGCGAGCAAACCGCACTATGCCAGGCAGT
ATTCGATTGCGAGCCCGCGCAACGGCGAGCGGCCCGGTTACAACAATGTGTCGCTCACCATCAAGCGCGTGCTGCAAGAC
CACCAGGGCAACGCGGTGCGCGGTGTCGGGTCGAACTACATGTGTGACCTGCAGATCGGCGACAAGGTGCAGGTGATCGG
CCCGTTCGGCACCAGCTTCCTGATGCCCAACCACCCGCGGTCGAACATCGTGATGATTTGCACCGGCACGGGCAGCGCAC
CCATGCGCGCCATGACCGAATGGCGTCGCCGGCTGCGGCAATCGGGCAAGTTTGAAGGGGGCAAGCTGATGCTGTTCTTT
GGCGCACGCACCAAGGAGGAGTTGCCGTATTTTGGCCCGCTCCAGACCCTGCCCAAGGATTTCATCGACATCAACTTCGC
TTTTTCGCGCACGCTGGAGCAACCCAAGCGCTACGTGCAGGATGCGATGCGCGAGCGGGCCGCCGACCTGGGCGTCTTGC
TCAAAGACCCTAACACCTACTTCTATGTGTGTGGACTCAAGAGCATGGAAGAGGGCGTGGTGCTGGCCTTGCGCGATGTG
GCGCAGCAGGCTGGCCTGTCGTGGGAAACGGTGGGTGCGGCCCTGAAACAGGAAGGTCGGCTCCATCTTGAAACCTACTG
A

Upstream 100 bases:

>100_bases
GACTACGTCCGTTTTAACTGATCTCCCCCAGCAAGGAAGATCATGCAGGGGGCACTTGGCGCCTCCTGCATGCCAATCAA
GCCGATGACCGGGAACCGCC

Downstream 100 bases:

>100_bases
GCGATAGGCAAAGCAATAAGCAAAGCAATAAGCAACAAGCTCGCAATAAGCAGCTCCCCACCCACTTTTTTTGCCGGTAA
GAAGGCCTATTGATGTTTCC

Product: benzoyl-CoA oxygenase, component A

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit A; Benzoyl-CoA dioxygenase reductase component [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MDMTEIHVIKQHLIDPEICIRCNTCEAICPVQAITHDSRNYVVDAEKCNLCMACISPCPTGSIDNWRTMPRIKAYSLTEQ
LGWDELPPELTPEELAAEGVVPGAMPEIEPVAPSLPAAVSGETAFNSAQYGATMPPWSAAHGYTNLYGAKAAEKFITATV
VGNVRVTEVGKEYDTHHIVLDFGSMPFPVLEGQSIGILPPGTDEASKPHYARQYSIASPRNGERPGYNNVSLTIKRVLQD
HQGNAVRGVGSNYMCDLQIGDKVQVIGPFGTSFLMPNHPRSNIVMICTGTGSAPMRAMTEWRRRLRQSGKFEGGKLMLFF
GARTKEELPYFGPLQTLPKDFIDINFAFSRTLEQPKRYVQDAMRERAADLGVLLKDPNTYFYVCGLKSMEEGVVLALRDV
AQQAGLSWETVGAALKQEGRLHLETY

Sequences:

>Translated_426_residues
MDMTEIHVIKQHLIDPEICIRCNTCEAICPVQAITHDSRNYVVDAEKCNLCMACISPCPTGSIDNWRTMPRIKAYSLTEQ
LGWDELPPELTPEELAAEGVVPGAMPEIEPVAPSLPAAVSGETAFNSAQYGATMPPWSAAHGYTNLYGAKAAEKFITATV
VGNVRVTEVGKEYDTHHIVLDFGSMPFPVLEGQSIGILPPGTDEASKPHYARQYSIASPRNGERPGYNNVSLTIKRVLQD
HQGNAVRGVGSNYMCDLQIGDKVQVIGPFGTSFLMPNHPRSNIVMICTGTGSAPMRAMTEWRRRLRQSGKFEGGKLMLFF
GARTKEELPYFGPLQTLPKDFIDINFAFSRTLEQPKRYVQDAMRERAADLGVLLKDPNTYFYVCGLKSMEEGVVLALRDV
AQQAGLSWETVGAALKQEGRLHLETY
>Mature_426_residues
MDMTEIHVIKQHLIDPEICIRCNTCEAICPVQAITHDSRNYVVDAEKCNLCMACISPCPTGSIDNWRTMPRIKAYSLTEQ
LGWDELPPELTPEELAAEGVVPGAMPEIEPVAPSLPAAVSGETAFNSAQYGATMPPWSAAHGYTNLYGAKAAEKFITATV
VGNVRVTEVGKEYDTHHIVLDFGSMPFPVLEGQSIGILPPGTDEASKPHYARQYSIASPRNGERPGYNNVSLTIKRVLQD
HQGNAVRGVGSNYMCDLQIGDKVQVIGPFGTSFLMPNHPRSNIVMICTGTGSAPMRAMTEWRRRLRQSGKFEGGKLMLFF
GARTKEELPYFGPLQTLPKDFIDINFAFSRTLEQPKRYVQDAMRERAADLGVLLKDPNTYFYVCGLKSMEEGVVLALRDV
AQQAGLSWETVGAALKQEGRLHLETY

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=226, Percent_Identity=30.9734513274336, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI10835173, Length=211, Percent_Identity=27.9620853080569, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789123, Length=229, Percent_Identity=31.8777292576419, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321143, Length=231, Percent_Identity=31.6017316017316, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583543, Length=231, Percent_Identity=30.3030303030303, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706876, Length=231, Percent_Identity=30.3030303030303, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706872, Length=231, Percent_Identity=30.3030303030303, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017634
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012146
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00037 Fer4; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 46974; Mature: 46974

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMTEIHVIKQHLIDPEICIRCNTCEAICPVQAITHDSRNYVVDAEKCNLCMACISPCPT
CCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCEEEEHHHHCCHHHHHCCCCC
GSIDNWRTMPRIKAYSLTEQLGWDELPPELTPEELAAEGVVPGAMPEIEPVAPSLPAAVS
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GETAFNSAQYGATMPPWSAAHGYTNLYGAKAAEKFITATVVGNVRVTEVGKEYDTHHIVL
CCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHEEEEEECCEEEEECCCCCCCEEEEE
DFGSMPFPVLEGQSIGILPPGTDEASKPHYARQYSIASPRNGERPGYNNVSLTIKRVLQD
ECCCCCCCEECCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
HQGNAVRGVGSNYMCDLQIGDKVQVIGPFGTSFLMPNHPRSNIVMICTGTGSAPMRAMTE
CCCCEEEECCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
WRRRLRQSGKFEGGKLMLFFGARTKEELPYFGPLQTLPKDFIDINFAFSRTLEQPKRYVQ
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHCCHHHEEEEHHHHHHHHHHHHHHH
DAMRERAADLGVLLKDPNTYFYVCGLKSMEEGVVLALRDVAQQAGLSWETVGAALKQEGR
HHHHHHHHHCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
LHLETY
EEEECC
>Mature Secondary Structure
MDMTEIHVIKQHLIDPEICIRCNTCEAICPVQAITHDSRNYVVDAEKCNLCMACISPCPT
CCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHCCCCCCEEEEHHHHCCHHHHHCCCCC
GSIDNWRTMPRIKAYSLTEQLGWDELPPELTPEELAAEGVVPGAMPEIEPVAPSLPAAVS
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GETAFNSAQYGATMPPWSAAHGYTNLYGAKAAEKFITATVVGNVRVTEVGKEYDTHHIVL
CCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHEEEEEECCEEEEECCCCCCCEEEEE
DFGSMPFPVLEGQSIGILPPGTDEASKPHYARQYSIASPRNGERPGYNNVSLTIKRVLQD
ECCCCCCCEECCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
HQGNAVRGVGSNYMCDLQIGDKVQVIGPFGTSFLMPNHPRSNIVMICTGTGSAPMRAMTE
CCCCEEEECCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
WRRRLRQSGKFEGGKLMLFFGARTKEELPYFGPLQTLPKDFIDINFAFSRTLEQPKRYVQ
HHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHCCHHHEEEEHHHHHHHHHHHHHHH
DAMRERAADLGVLLKDPNTYFYVCGLKSMEEGVVLALRDVAQQAGLSWETVGAALKQEGR
HHHHHHHHHCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCC
LHLETY
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]