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The map label for this gene is paaF [C]

Identifier: 91787513

GI number: 91787513

Start: 1685574

End: 1686404

Strand: Direct

Name: paaF [C]

Synonym: Bpro_1631

Alternate gene names: 91787513

Gene position: 1685574-1686404 (Clockwise)

Preceding gene: 91787512

Following gene: 91787514

Centisome position: 32.41

GC content: 67.15

Gene sequence:

>831_bases
ATGAAAGTCTCCATAGCGCCTTCCGGCAACCGTTCGAGTCATGCCATGAAAAATCTGACCCTCTCATCCCGCGCCCCCGG
CGTGGCTCAAGTCACCATGTCCCGCCCGGCGGTGTTCAACGCCTTTGACGAGGCGATGATCACTGAACTGGAAACGGCCT
TCACCCAGCTTGCGGCCGATCCGGCCGTGCGCGTGATCGTGCTGGCGGGCGAAGGCCGGCATTTCAGCGCCGGTGCCGAT
CTGCAATGGATGCAGCGCGCCAGCACCGCCTCCCGCGAATGGAACCTGGACGATGCGCGCCGCTTTGCCGGCATGCTGGC
GTGCATTGAGTCGTGCCCCAAGCCCACGGTGGCAAGGGTGCAGGGCGCGGCGTTGGGTGGCGGCGTGGGCCTGGTCTGCG
CCTGCGACCTGGCGATTGCGGCCGACAACGCCAGCTTCGCGGTCAGCGAGGCGAGGTTCGGCATCTTGCCCGCGGTGATC
GGCCCCTATGTGGTCAACGCGGTGGGCAGGCGCCACGCCAGGCGCCTGGCGCTGACGACCACGCGCATCCCTGCCGCCGA
GGCACTGGCCATCGGGCTGGTGCAGCAGGTGGTTGGGCTGGACGCGCTCGATGCGGCGCTGGATGCCGCCATTGCCGAGC
TGCTGGTCGGCGGGCCAGGCGCACAGCGTGAAATCAAGCAGCTGTTTGCCCAGCTTGAAGTCGGGCCGATCACGAATGAG
GTGCGCGAGTTGACCGCGCAAACGATCAGCCGCGTGCGGGGTACCGACGAGGCCCGCGAAGGCTTTGCGGCCTTTCTGGG
AAAGCGGCCACCGAACTGGATGGCGCAATGA

Upstream 100 bases:

>100_bases
ACACCGTTCGGGCTGAGCCTGGCCTGTCCTGAGCTTGTCGAAGTGGCGAAGCCCTTCGACAGGCTCAGGGCGAACGGTAG
CGAAGTGCCTGTCAAGATTT

Downstream 100 bases:

>100_bases
CCATGAACTCGACAGTCAACAAGCTCGACGAGGCGCCCCTGCTGGTGGTCGGCGCCGGTGTCATGGGGGTCGGTATTGCG
CAGGTGGCGGCACAGGCCGG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKVSIAPSGNRSSHAMKNLTLSSRAPGVAQVTMSRPAVFNAFDEAMITELETAFTQLAADPAVRVIVLAGEGRHFSAGAD
LQWMQRASTASREWNLDDARRFAGMLACIESCPKPTVARVQGAALGGGVGLVCACDLAIAADNASFAVSEARFGILPAVI
GPYVVNAVGRRHARRLALTTTRIPAAEALAIGLVQQVVGLDALDAALDAAIAELLVGGPGAQREIKQLFAQLEVGPITNE
VRELTAQTISRVRGTDEAREGFAAFLGKRPPNWMAQ

Sequences:

>Translated_276_residues
MKVSIAPSGNRSSHAMKNLTLSSRAPGVAQVTMSRPAVFNAFDEAMITELETAFTQLAADPAVRVIVLAGEGRHFSAGAD
LQWMQRASTASREWNLDDARRFAGMLACIESCPKPTVARVQGAALGGGVGLVCACDLAIAADNASFAVSEARFGILPAVI
GPYVVNAVGRRHARRLALTTTRIPAAEALAIGLVQQVVGLDALDAALDAAIAELLVGGPGAQREIKQLFAQLEVGPITNE
VRELTAQTISRVRGTDEAREGFAAFLGKRPPNWMAQ
>Mature_276_residues
MKVSIAPSGNRSSHAMKNLTLSSRAPGVAQVTMSRPAVFNAFDEAMITELETAFTQLAADPAVRVIVLAGEGRHFSAGAD
LQWMQRASTASREWNLDDARRFAGMLACIESCPKPTVARVQGAALGGGVGLVCACDLAIAADNASFAVSEARFGILPAVI
GPYVVNAVGRRHARRLALTTTRIPAAEALAIGLVQQVVGLDALDAALDAAIAELLVGGPGAQREIKQLFAQLEVGPITNE
VRELTAQTISRVRGTDEAREGFAAFLGKRPPNWMAQ

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=30.1587301587302, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=28.4046692607004, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI37594469, Length=183, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594471, Length=183, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=32.6923076923077, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI68989263, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI260274832, Length=270, Percent_Identity=23.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45643119, Length=195, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI260275230, Length=195, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI157694516, Length=214, Percent_Identity=24.7663551401869, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI213417737, Length=214, Percent_Identity=24.7663551401869, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=33.0708661417323, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788597, Length=268, Percent_Identity=30.5970149253731, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI221142681, Length=269, Percent_Identity=32.3420074349442, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=29.5880149812734, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=25.4826254826255, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=28.3582089552239, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=271, Percent_Identity=30.2583025830258, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=204, Percent_Identity=32.843137254902, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=284, Percent_Identity=27.8169014084507, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=203, Percent_Identity=27.0935960591133, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=196, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=30.2197802197802, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=27.9166666666667, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=166, Percent_Identity=30.7228915662651, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=30.5263157894737, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=25.0996015936255, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=156, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=156, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=156, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19920382, Length=196, Percent_Identity=35.2040816326531, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653139, Length=287, Percent_Identity=29.9651567944251, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=22.6415094339623, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29028; Mature: 29028

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSIAPSGNRSSHAMKNLTLSSRAPGVAQVTMSRPAVFNAFDEAMITELETAFTQLAAD
CEEECCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
PAVRVIVLAGEGRHFSAGADLQWMQRASTASREWNLDDARRFAGMLACIESCPKPTVARV
CCEEEEEEECCCCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QGAALGGGVGLVCACDLAIAADNASFAVSEARFGILPAVIGPYVVNAVGRRHARRLALTT
CCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRIPAAEALAIGLVQQVVGLDALDAALDAAIAELLVGGPGAQREIKQLFAQLEVGPITNE
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHH
VRELTAQTISRVRGTDEAREGFAAFLGKRPPNWMAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKVSIAPSGNRSSHAMKNLTLSSRAPGVAQVTMSRPAVFNAFDEAMITELETAFTQLAAD
CEEECCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
PAVRVIVLAGEGRHFSAGADLQWMQRASTASREWNLDDARRFAGMLACIESCPKPTVARV
CCEEEEEEECCCCEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QGAALGGGVGLVCACDLAIAADNASFAVSEARFGILPAVIGPYVVNAVGRRHARRLALTT
CCCCCCCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRIPAAEALAIGLVQQVVGLDALDAALDAAIAELLVGGPGAQREIKQLFAQLEVGPITNE
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHH
VRELTAQTISRVRGTDEAREGFAAFLGKRPPNWMAQ
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA