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The map label for this gene is bclA [H]

Identifier: 91787506

GI number: 91787506

Start: 1675817

End: 1677397

Strand: Reverse

Name: bclA [H]

Synonym: Bpro_1624

Alternate gene names: 91787506

Gene position: 1677397-1675817 (Counterclockwise)

Preceding gene: 91787507

Following gene: 91787505

Centisome position: 32.26

GC content: 65.02

Gene sequence:

>1581_bases
TTGACCCCCACCCTCCCCGTCGCGCCGCCCGGCGCGCGCTTTAACTTTGCCCACCACCTGATCGAGCGCAACCTGGCCCG
ACCCGCCAAACCAGCCTACATTGACGACCAGGGCACCCTGAGCTACGGCGATCTGGCCGGGCACATTCGCCGTCTGGCTG
CAGCGCTGCTGGCCTCGGGCGTGCGCCGCGAAGAGCGCGTGCTGCTGCTGATGCACGACTGCAGCGACTGGCCGGTGAGC
TTTCTGGGCGCCATGTACGCCGGCATCGTGCCGGTGGCCGTCAACACACTGCTCACCGCCGACGACTACGCCTACATGCT
GCAACACAGCCGGGCACAGGCGGCCATCGTGTCCGGGGCCTTGCTGCCCACGCTGCAGGCGGCCATGGTCCAGGGTGGCC
ACGAGCTCAAAACCGTGATCGTCTCACGCCCGGACGCGGCCTTGCCCGAGGGCGCCGTGGCACTCGACACCTTGATGGAA
CAACACGCGCCACTGGCCGAACCGGCGGGCACCAGCCCGGACGACCCCGGCTTCTGGCTCTATTCCTCCGGCTCCACCGG
TAGACCCAAAGGCACGCTGCACAGCCACGGCAATCCTTACTGGACCGCCGAGCTCTATGGCACGCCGGTGCTGGGCCTCA
CCGAGCAGGATGTCTGCTTCTCGGCGGCCAAACTTTTCTTTGCCTATGGCCTGGGCAATGCGCTGAGTTTTCCCTTGAGC
GTGGGCGCCACCACGATCCTGATGGCCGAGCGGCCAACACCGGAGGCCACGTTCAAACGCTGGCGCGGCGAGGTGGGCGG
CATGCGTCCAACCGTATTCTTCGGTGCGCCCACCGGTTTTGCCGGCATGCTGGCCTCGCCTGCCCTGCCGGCTAAAACAG
ACATTGCGCTGCGCCTGGTCTCCTCGGCCGGCGAAGCCTTGCCCAGCGAACTGGGCGAGCGGTTCACGGCCCACTTCGGG
GTCGAGATCATCGACGGCCTGGGTTCGACCGAAATGCTGCATATCTTCCTGTCCAACCGCCCGGGACAGGTGCGCTATGG
CACCACGGGCTGGCCCGTGCCAGGGTATGAGGTCGAGCTGCGGGACGACGACGGCCGCCCGGTGCCCGATGGCGAAACCG
GCGACCTCTACATCCACGGCCCAAGTTCGGCACTCATGTACTGGGGCAACCGCGAGAAATCGCGCGAGACCTTTCAGGGT
GCGTGGACCAAGAGCGGCGACAAATACGTGCGCAACGCCGACGGCACTTACACCTACTCGGGCCGCAGTGACGACATGCT
CAAGGTCAGCGGCATTTATGTGTCGCCGTTCGAGGTCGAGGCCACGCTGGTGCAGCACCCGGCAGTGCTGGAGTGCGCCG
TGATCGGCAAGGAAGATGAAAAAGGCTTGACCAAGACGAAGGCCTTTGTCGTGGTCAAACCAGGCCATCAGGTCGACGAG
GCCACCCTGCAAGCTTTTGTGAAAGAGCGGCTCGCGCCCTACAAGTACCCGCGTTTCATCGAGTTCATGACCGAACTGCC
GAAAACGGCAACGGGAAAAATCCAGCGCTTTCGCTTGCGGGAACTGGAGCGCAAGGCATGA

Upstream 100 bases:

>100_bases
TGAACTTCTACCACCGCCGCAGCGCGGTGCAAGCCAGCACCGCCGTGCTCAAAACCCTCTAAGCACCGCCGACTGCCCTG
CAAAAATCAAGGAGACAGAC

Downstream 100 bases:

>100_bases
GTGCAGCGCAGCCACTTCAAACAACCATGAGCGCCACCGAATTCGTCGACATCGACTGGGCAGGTCGGCCGGTGCGTATC
GAGCATCAATGGGTCAACGC

Product: benzoate-CoA ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTPTLPVAPPGARFNFAHHLIERNLARPAKPAYIDDQGTLSYGDLAGHIRRLAAALLASGVRREERVLLLMHDCSDWPVS
FLGAMYAGIVPVAVNTLLTADDYAYMLQHSRAQAAIVSGALLPTLQAAMVQGGHELKTVIVSRPDAALPEGAVALDTLME
QHAPLAEPAGTSPDDPGFWLYSSGSTGRPKGTLHSHGNPYWTAELYGTPVLGLTEQDVCFSAAKLFFAYGLGNALSFPLS
VGATTILMAERPTPEATFKRWRGEVGGMRPTVFFGAPTGFAGMLASPALPAKTDIALRLVSSAGEALPSELGERFTAHFG
VEIIDGLGSTEMLHIFLSNRPGQVRYGTTGWPVPGYEVELRDDDGRPVPDGETGDLYIHGPSSALMYWGNREKSRETFQG
AWTKSGDKYVRNADGTYTYSGRSDDMLKVSGIYVSPFEVEATLVQHPAVLECAVIGKEDEKGLTKTKAFVVVKPGHQVDE
ATLQAFVKERLAPYKYPRFIEFMTELPKTATGKIQRFRLRELERKA

Sequences:

>Translated_526_residues
MTPTLPVAPPGARFNFAHHLIERNLARPAKPAYIDDQGTLSYGDLAGHIRRLAAALLASGVRREERVLLLMHDCSDWPVS
FLGAMYAGIVPVAVNTLLTADDYAYMLQHSRAQAAIVSGALLPTLQAAMVQGGHELKTVIVSRPDAALPEGAVALDTLME
QHAPLAEPAGTSPDDPGFWLYSSGSTGRPKGTLHSHGNPYWTAELYGTPVLGLTEQDVCFSAAKLFFAYGLGNALSFPLS
VGATTILMAERPTPEATFKRWRGEVGGMRPTVFFGAPTGFAGMLASPALPAKTDIALRLVSSAGEALPSELGERFTAHFG
VEIIDGLGSTEMLHIFLSNRPGQVRYGTTGWPVPGYEVELRDDDGRPVPDGETGDLYIHGPSSALMYWGNREKSRETFQG
AWTKSGDKYVRNADGTYTYSGRSDDMLKVSGIYVSPFEVEATLVQHPAVLECAVIGKEDEKGLTKTKAFVVVKPGHQVDE
ATLQAFVKERLAPYKYPRFIEFMTELPKTATGKIQRFRLRELERKA
>Mature_525_residues
TPTLPVAPPGARFNFAHHLIERNLARPAKPAYIDDQGTLSYGDLAGHIRRLAAALLASGVRREERVLLLMHDCSDWPVSF
LGAMYAGIVPVAVNTLLTADDYAYMLQHSRAQAAIVSGALLPTLQAAMVQGGHELKTVIVSRPDAALPEGAVALDTLMEQ
HAPLAEPAGTSPDDPGFWLYSSGSTGRPKGTLHSHGNPYWTAELYGTPVLGLTEQDVCFSAAKLFFAYGLGNALSFPLSV
GATTILMAERPTPEATFKRWRGEVGGMRPTVFFGAPTGFAGMLASPALPAKTDIALRLVSSAGEALPSELGERFTAHFGV
EIIDGLGSTEMLHIFLSNRPGQVRYGTTGWPVPGYEVELRDDDGRPVPDGETGDLYIHGPSSALMYWGNREKSRETFQGA
WTKSGDKYVRNADGTYTYSGRSDDMLKVSGIYVSPFEVEATLVQHPAVLECAVIGKEDEKGLTKTKAFVVVKPGHQVDEA
TLQAFVKERLAPYKYPRFIEFMTELPKTATGKIQRFRLRELERKA

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=533, Percent_Identity=30.0187617260788, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=29.1588785046729, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI122937307, Length=541, Percent_Identity=28.4658040665434, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI58082049, Length=539, Percent_Identity=27.4582560296846, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI115511026, Length=532, Percent_Identity=28.3834586466165, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI157311624, Length=539, Percent_Identity=27.0871985157699, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI157311622, Length=539, Percent_Identity=27.0871985157699, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI187761345, Length=552, Percent_Identity=27.3550724637681, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI187761343, Length=552, Percent_Identity=27.3550724637681, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI156151445, Length=524, Percent_Identity=26.7175572519084, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI28416953, Length=554, Percent_Identity=27.4368231046931, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI8923896, Length=578, Percent_Identity=24.7404844290657, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI253970489, Length=384, Percent_Identity=29.4270833333333, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI13375727, Length=558, Percent_Identity=24.1935483870968, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI42544134, Length=366, Percent_Identity=25.1366120218579, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI38524616, Length=490, Percent_Identity=25.9183673469388, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788107, Length=543, Percent_Identity=28.1767955801105, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=27.3833671399594, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI145693145, Length=506, Percent_Identity=26.8774703557312, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1790505, Length=548, Percent_Identity=25.9124087591241, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1786810, Length=509, Percent_Identity=23.7721021611002, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786529, Length=566, Percent_Identity=25.9717314487633, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1789201, Length=358, Percent_Identity=27.9329608938547, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786801, Length=537, Percent_Identity=25.6983240223464, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788595, Length=388, Percent_Identity=23.7113402061856, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=503, Percent_Identity=27.4353876739563, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=507, Percent_Identity=24.4575936883629, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=524, Percent_Identity=24.8091603053435, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=524, Percent_Identity=24.8091603053435, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=524, Percent_Identity=24.8091603053435, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=26.7657992565056, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=527, Percent_Identity=24.4781783681214, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=463, Percent_Identity=26.9978401727862, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=26.0299625468165, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983001, Length=561, Percent_Identity=25.4901960784314, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71982997, Length=561, Percent_Identity=25.4901960784314, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=509, Percent_Identity=24.5579567779961, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=519, Percent_Identity=24.2774566473988, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=516, Percent_Identity=25.1937984496124, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=23.1182795698925, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323182, Length=568, Percent_Identity=25.3521126760563, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6319699, Length=508, Percent_Identity=24.6062992125984, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=374, Percent_Identity=28.3422459893048, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859661, Length=477, Percent_Identity=28.9308176100629, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24667955, Length=563, Percent_Identity=26.2877442273535, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI62472339, Length=552, Percent_Identity=26.4492753623188, Blast_Score=147, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24581924, Length=517, Percent_Identity=26.1121856866538, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24667959, Length=442, Percent_Identity=27.1493212669683, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20130353, Length=560, Percent_Identity=23.5714285714286, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI28573631, Length=560, Percent_Identity=23.5714285714286, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21355181, Length=497, Percent_Identity=26.3581488933602, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648260, Length=495, Percent_Identity=25.2525252525253, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21355225, Length=567, Percent_Identity=23.1040564373898, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI20130357, Length=516, Percent_Identity=23.062015503876, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653035, Length=352, Percent_Identity=25, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648255, Length=494, Percent_Identity=23.0769230769231, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648253, Length=494, Percent_Identity=22.8744939271255, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI161076582, Length=358, Percent_Identity=23.7430167597765, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=374, Percent_Identity=22.4598930481283, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356947, Length=353, Percent_Identity=26.3456090651558, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648676, Length=375, Percent_Identity=25.6, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 57097; Mature: 56965

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTLPVAPPGARFNFAHHLIERNLARPAKPAYIDDQGTLSYGDLAGHIRRLAAALLASG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCEEHHHHHHHHHHHHHHHHHHC
VRREERVLLLMHDCSDWPVSFLGAMYAGIVPVAVNTLLTADDYAYMLQHSRAQAAIVSGA
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHH
LLPTLQAAMVQGGHELKTVIVSRPDAALPEGAVALDTLMEQHAPLAEPAGTSPDDPGFWL
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
YSSGSTGRPKGTLHSHGNPYWTAELYGTPVLGLTEQDVCFSAAKLFFAYGLGNALSFPLS
ECCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHCCCHH
VGATTILMAERPTPEATFKRWRGEVGGMRPTVFFGAPTGFAGMLASPALPAKTDIALRLV
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHH
SSAGEALPSELGERFTAHFGVEIIDGLGSTEMLHIFLSNRPGQVRYGTTGWPVPGYEVEL
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEE
RDDDGRPVPDGETGDLYIHGPSSALMYWGNREKSRETFQGAWTKSGDKYVRNADGTYTYS
ECCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHCCCCCCCCCEEECCCCEEEEC
GRSDDMLKVSGIYVSPFEVEATLVQHPAVLECAVIGKEDEKGLTKTKAFVVVKPGHQVDE
CCCCCEEEEEEEEECCCEEEEHHHCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCHHH
ATLQAFVKERLAPYKYPRFIEFMTELPKTATGKIQRFRLRELERKA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPTLPVAPPGARFNFAHHLIERNLARPAKPAYIDDQGTLSYGDLAGHIRRLAAALLASG
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCEEHHHHHHHHHHHHHHHHHHC
VRREERVLLLMHDCSDWPVSFLGAMYAGIVPVAVNTLLTADDYAYMLQHSRAQAAIVSGA
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHH
LLPTLQAAMVQGGHELKTVIVSRPDAALPEGAVALDTLMEQHAPLAEPAGTSPDDPGFWL
HHHHHHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
YSSGSTGRPKGTLHSHGNPYWTAELYGTPVLGLTEQDVCFSAAKLFFAYGLGNALSFPLS
ECCCCCCCCCCCCCCCCCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHCCCHH
VGATTILMAERPTPEATFKRWRGEVGGMRPTVFFGAPTGFAGMLASPALPAKTDIALRLV
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHH
SSAGEALPSELGERFTAHFGVEIIDGLGSTEMLHIFLSNRPGQVRYGTTGWPVPGYEVEL
HHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEE
RDDDGRPVPDGETGDLYIHGPSSALMYWGNREKSRETFQGAWTKSGDKYVRNADGTYTYS
ECCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHCCCCCCCCCEEECCCCEEEEC
GRSDDMLKVSGIYVSPFEVEATLVQHPAVLECAVIGKEDEKGLTKTKAFVVVKPGHQVDE
CCCCCEEEEEEEEECCCEEEEHHHCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCHHH
ATLQAFVKERLAPYKYPRFIEFMTELPKTATGKIQRFRLRELERKA
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]