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The map label for this gene is 91787391

Identifier: 91787391

GI number: 91787391

Start: 1552787

End: 1553752

Strand: Reverse

Name: 91787391

Synonym: Bpro_1497

Alternate gene names: NA

Gene position: 1553752-1552787 (Counterclockwise)

Preceding gene: 91787392

Following gene: 91787383

Centisome position: 29.88

GC content: 63.98

Gene sequence:

>966_bases
ATGCCACGCTACATCAACGTCGCCCTGGGCCAGTTGGGCCCCATCCAGCGCAATGACACCCGCGCCCAAGTGGTCGGGCG
CCTGTGCGCACTCATGCGCCAGGCCCACGCGGTGGGGGCGCAGCTCATCGTGTATCCCGAACTGGCGCTGACCACCTTTT
TCCCGCGCTGGTACATCGAAGATCCACAGGAGATCAACCAGTACTTCGAGCGTGAGATGCCCAGCGCCGCCACCCAACCC
CTGTTCAGCCTGGCGCAGGAGCTGGGTGTGGGCTTTTACCTGGGTTACGCCGAGCTGGCACAGGAGGCCGGCGCGGAGCT
GCGCTACAACACCTCCATTCTGGTCGACCGCTTTGGGCAGATCGTGGCCAAGTACCGCAAGGTGCATCTGCCAGGTCACA
AAGAGCATGAACCCTGGCGGCGCTTTCAGCACCTGGAAAAGCGCTACTTCACACCCGGACCCGGCTTTGGTGTGACAAAC
GCTTTCGGCGGCGTGATGGGCATGGCCATTTGCAACGACCGGCGCTGGGCCGAAACCTACCGCGTGATGGGCCTGCAAGG
GGTGGAGATGGTGCTGATTGGCTACAACACCCCGGTGCACAACGCCCCCGCTCCCGAGCATGACGACCTTTCACTGTTTC
ACAACCAGCTGTCCATGCAGGCCGGTGCCTACCAGAACGGCACCTGGGTGGTGGGGGTGGCCAAAGCCGGGGTGGAAGAA
GGCGTGGACAGCATAGGCGGCAGCTGCATCATCGCCCCCTCTGGCGAGGTGGTGGCCCGCTGCCTCACCAAGGGCGACGA
AATGGCCATCGCCCGTTGCGACCTGGACTTCTGCGCCGTGTACAAGCGCACCACCTTCAACTTCGATATGCACCGCCAGC
CCCATGCCTATGGCCTGATCGTCGAGCGCAAGGGGGAGACCCTGGCGGCAGATGGCAGCCCGCTGCCTGTGCCACCAGCC
GATTGA

Upstream 100 bases:

>100_bases
CGTGCGCGAACACACCGAACCCGACGACCTGCTGCGCGGAGCCCAGTTGCTGCTCCAGGTGGTGGCCGAACTTGCCAACC
GAACTTCATAGTCTTTAAAC

Downstream 100 bases:

>100_bases
ACTTGCCATCGTTTGTATAGCTTCTTGCGCAATTTTTGCACGGACTGCAAGGGGTCTATGCTTACAACATCGGCAATCAG
GCTGTTCCTGCCTGCGAAAT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MPRYINVALGQLGPIQRNDTRAQVVGRLCALMRQAHAVGAQLIVYPELALTTFFPRWYIEDPQEINQYFEREMPSAATQP
LFSLAQELGVGFYLGYAELAQEAGAELRYNTSILVDRFGQIVAKYRKVHLPGHKEHEPWRRFQHLEKRYFTPGPGFGVTN
AFGGVMGMAICNDRRWAETYRVMGLQGVEMVLIGYNTPVHNAPAPEHDDLSLFHNQLSMQAGAYQNGTWVVGVAKAGVEE
GVDSIGGSCIIAPSGEVVARCLTKGDEMAIARCDLDFCAVYKRTTFNFDMHRQPHAYGLIVERKGETLAADGSPLPVPPA
D

Sequences:

>Translated_321_residues
MPRYINVALGQLGPIQRNDTRAQVVGRLCALMRQAHAVGAQLIVYPELALTTFFPRWYIEDPQEINQYFEREMPSAATQP
LFSLAQELGVGFYLGYAELAQEAGAELRYNTSILVDRFGQIVAKYRKVHLPGHKEHEPWRRFQHLEKRYFTPGPGFGVTN
AFGGVMGMAICNDRRWAETYRVMGLQGVEMVLIGYNTPVHNAPAPEHDDLSLFHNQLSMQAGAYQNGTWVVGVAKAGVEE
GVDSIGGSCIIAPSGEVVARCLTKGDEMAIARCDLDFCAVYKRTTFNFDMHRQPHAYGLIVERKGETLAADGSPLPVPPA
D
>Mature_320_residues
PRYINVALGQLGPIQRNDTRAQVVGRLCALMRQAHAVGAQLIVYPELALTTFFPRWYIEDPQEINQYFEREMPSAATQPL
FSLAQELGVGFYLGYAELAQEAGAELRYNTSILVDRFGQIVAKYRKVHLPGHKEHEPWRRFQHLEKRYFTPGPGFGVTNA
FGGVMGMAICNDRRWAETYRVMGLQGVEMVLIGYNTPVHNAPAPEHDDLSLFHNQLSMQAGAYQNGTWVVGVAKAGVEEG
VDSIGGSCIIAPSGEVVARCLTKGDEMAIARCDLDFCAVYKRTTFNFDMHRQPHAYGLIVERKGETLAADGSPLPVPPAD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=269, Percent_Identity=24.907063197026, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI5031947, Length=282, Percent_Identity=29.0780141843972, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI297632348, Length=282, Percent_Identity=29.0780141843972, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI297632350, Length=282, Percent_Identity=29.0780141843972, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 35617; Mature: 35486

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYINVALGQLGPIQRNDTRAQVVGRLCALMRQAHAVGAQLIVYPELALTTFFPRWYIE
CCCEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCC
DPQEINQYFEREMPSAATQPLFSLAQELGVGFYLGYAELAQEAGAELRYNTSILVDRFGQ
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECHHHHHHHCCCCEEECHHHHHHHHHH
IVAKYRKVHLPGHKEHEPWRRFQHLEKRYFTPGPGFGVTNAFGGVMGMAICNDRRWAETY
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
RVMGLQGVEMVLIGYNTPVHNAPAPEHDDLSLFHNQLSMQAGAYQNGTWVVGVAKAGVEE
HHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHCHHH
GVDSIGGSCIIAPSGEVVARCLTKGDEMAIARCDLDFCAVYKRTTFNFDMHRQPHAYGLI
HHHHCCCCEEEECCHHHHHHHHCCCCCEEEEECCCHHHHHHHHCEECCCCCCCCCEEEEE
VERKGETLAADGSPLPVPPAD
EEECCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
PRYINVALGQLGPIQRNDTRAQVVGRLCALMRQAHAVGAQLIVYPELALTTFFPRWYIE
CCEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCC
DPQEINQYFEREMPSAATQPLFSLAQELGVGFYLGYAELAQEAGAELRYNTSILVDRFGQ
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECHHHHHHHCCCCEEECHHHHHHHHHH
IVAKYRKVHLPGHKEHEPWRRFQHLEKRYFTPGPGFGVTNAFGGVMGMAICNDRRWAETY
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
RVMGLQGVEMVLIGYNTPVHNAPAPEHDDLSLFHNQLSMQAGAYQNGTWVVGVAKAGVEE
HHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEHHCHHH
GVDSIGGSCIIAPSGEVVARCLTKGDEMAIARCDLDFCAVYKRTTFNFDMHRQPHAYGLI
HHHHCCCCEEEECCHHHHHHHHCCCCCEEEEECCCHHHHHHHHCEECCCCCCCCCEEEEE
VERKGETLAADGSPLPVPPAD
EEECCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]