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The map label for this gene is rhmD

Identifier: 91787387

GI number: 91787387

Start: 1548865

End: 1550052

Strand: Direct

Name: rhmD

Synonym: Bpro_1493

Alternate gene names: 91787387

Gene position: 1548865-1550052 (Clockwise)

Preceding gene: 91787386

Following gene: 91787388

Centisome position: 29.78

GC content: 64.98

Gene sequence:

>1188_bases
ATGAACAACATGCCGACCATCAAGCACGTGCGTGCCTTCACCGTCCGCGGCGGCGGCGCCGACTACCACGACCAGGGCTC
CGGCCACTGGATCGACGACCATATCTCCACACCGATGGGGCGTTACCCCGAATATCGCCAGAGTCGCCAGTCCTTCGGCA
TCAATGTGCTGGGCACGCTGGTGGTGGAAATCGAGGCCAGCGACGGCACGGTGGGGTTTTCGGTGACGACCGGCGGTGAA
CTCGGCTGCTGGATCGTCGAGAAGCACCTGGCGCGCTTTATCGAAGGCGCCAAGGTCACCGACATCGAGAAGATCTGGGA
CCAGATGTTCAACGCCACGCTGTACTACGGGCGCAAGGGCATTGTGCTGAACACCATCTCGGGCGTGGACCTGGCGCTGT
GGGACCTGCTGGCCAAGGTGCGCAAGGAGCCGGTGCACGCCCTGCTGGGTGGCCCGGTGCGCGACGAATTGACCTTCTAC
GCCACCGGGGCCCGCCCCGACCTGGCCAAGAAAATGGGCTTCATCGGCGGCAAGCTGCCGCTGCACCATGGCCCCGCCGA
GCGGGAGGAGGGCCTGAAGAAAAACCTCGAACTGCTGGGCGAAATGCGCCAGCGCGTAGGCGACGATTTCTGGCTGATGT
ACGACTGCTGGATGAGCCTGGATGTAGAGTACGCCACGCGCCTGGCCAACGCGGCCAGCGAGTACAAGCTCAAGTGGATC
GAAGAAGCCCTGCCGCCCGACGACTACTGGGGCTACGCCGAGCTGCGCCGCAACGTGCCGCGCGGCATGCTGGTCACCAC
CGGCGAGCACGAAGCCACGCGCTGGGGCTTCCGCATGCTGCTCGAGATGGAGTGCTGCGACATTCTGCAGCCCGACGTCG
GCTGGTGCGGCGGCATCACCGAACTGCTGAAAATATCGGCACTGGCCGATGCACACGGCAAGCTGGTGGTGCCCCACGGC
TCGTCGGTCTACAGCTACCACTTCGTCATCACGCGCCACAACAGCCCGTTTTCCGAGTTTCTGATGATGGCGCCCAAGGC
CGACGAGGTGGTGCCGATGTTCAACCCCATGCTGCTGGACGAGCCGGTGCCGGTGAACGGCCGCATGAAGGCCAGCGCGC
TCGATGCGCCGGGCTTCGGCGTGCGGCTCAACCCCGAATGCGCCTTGCAGCGGCCATTCCCCCGCTGA

Upstream 100 bases:

>100_bases
GCGCTGCGCGCCCGGCAGCCCGTAGCAGTCGCAGTGGTCCGCTCGGCCTGACCCCGTTCACCTTCATCCAGCAGCAACTC
CAGAAGCAACACAAGCAATC

Downstream 100 bases:

>100_bases
CGCGGGCTTTACCGGCTGATCGGGCGCTGTCCGGCGGGAGGCGTTAAAATTGCAGGTTGCCAGCATGCTGTCCCGGCGTG
CTGGTGTTTGTTGTTCCTGA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNNMPTIKHVRAFTVRGGGADYHDQGSGHWIDDHISTPMGRYPEYRQSRQSFGINVLGTLVVEIEASDGTVGFSVTTGGE
LGCWIVEKHLARFIEGAKVTDIEKIWDQMFNATLYYGRKGIVLNTISGVDLALWDLLAKVRKEPVHALLGGPVRDELTFY
ATGARPDLAKKMGFIGGKLPLHHGPAEREEGLKKNLELLGEMRQRVGDDFWLMYDCWMSLDVEYATRLANAASEYKLKWI
EEALPPDDYWGYAELRRNVPRGMLVTTGEHEATRWGFRMLLEMECCDILQPDVGWCGGITELLKISALADAHGKLVVPHG
SSVYSYHFVITRHNSPFSEFLMMAPKADEVVPMFNPMLLDEPVPVNGRMKASALDAPGFGVRLNPECALQRPFPR

Sequences:

>Translated_395_residues
MNNMPTIKHVRAFTVRGGGADYHDQGSGHWIDDHISTPMGRYPEYRQSRQSFGINVLGTLVVEIEASDGTVGFSVTTGGE
LGCWIVEKHLARFIEGAKVTDIEKIWDQMFNATLYYGRKGIVLNTISGVDLALWDLLAKVRKEPVHALLGGPVRDELTFY
ATGARPDLAKKMGFIGGKLPLHHGPAEREEGLKKNLELLGEMRQRVGDDFWLMYDCWMSLDVEYATRLANAASEYKLKWI
EEALPPDDYWGYAELRRNVPRGMLVTTGEHEATRWGFRMLLEMECCDILQPDVGWCGGITELLKISALADAHGKLVVPHG
SSVYSYHFVITRHNSPFSEFLMMAPKADEVVPMFNPMLLDEPVPVNGRMKASALDAPGFGVRLNPECALQRPFPR
>Mature_395_residues
MNNMPTIKHVRAFTVRGGGADYHDQGSGHWIDDHISTPMGRYPEYRQSRQSFGINVLGTLVVEIEASDGTVGFSVTTGGE
LGCWIVEKHLARFIEGAKVTDIEKIWDQMFNATLYYGRKGIVLNTISGVDLALWDLLAKVRKEPVHALLGGPVRDELTFY
ATGARPDLAKKMGFIGGKLPLHHGPAEREEGLKKNLELLGEMRQRVGDDFWLMYDCWMSLDVEYATRLANAASEYKLKWI
EEALPPDDYWGYAELRRNVPRGMLVTTGEHEATRWGFRMLLEMECCDILQPDVGWCGGITELLKISALADAHGKLVVPHG
SSVYSYHFVITRHNSPFSEFLMMAPKADEVVPMFNPMLLDEPVPVNGRMKASALDAPGFGVRLNPECALQRPFPR

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR)

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily

Homologues:

Organism=Escherichia coli, GI226510960, Length=399, Percent_Identity=62.1553884711779, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI48994953, Length=275, Percent_Identity=27.6363636363636, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787864, Length=381, Percent_Identity=24.4094488188976, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHMD_POLSJ (Q12DF1)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548339.1
- ProteinModelPortal:   Q12DF1
- SMR:   Q12DF1
- STRING:   Q12DF1
- GeneID:   4015850
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1493
- NMPDR:   fig|296591.1.peg.1751
- eggNOG:   COG4948
- HOGENOM:   HBG705576
- OMA:   DEVVPMF
- PhylomeDB:   Q12DF1
- ProtClustDB:   PRK15440
- BioCyc:   PSP296591:BPRO_1493-MONOMER
- HAMAP:   MF_01288
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794
- SMART:   SM00922

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.90

Molecular weight: Translated: 44253; Mature: 44253

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 319-319 BINDING 23-23 BINDING 49-49 BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMPTIKHVRAFTVRGGGADYHDQGSGHWIDDHISTPMGRYPEYRQSRQSFGINVLGTL
CCCCCCCHHEEEEEEECCCCCCCCCCCCCEEHHHHCCCCCCCCHHHHHHHHCCCEEEEEE
VVEIEASDGTVGFSVTTGGELGCWIVEKHLARFIEGAKVTDIEKIWDQMFNATLYYGRKG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCC
IVLNTISGVDLALWDLLAKVRKEPVHALLGGPVRDELTFYATGARPDLAKKMGFIGGKLP
EEEEECCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCC
LHHGPAEREEGLKKNLELLGEMRQRVGDDFWLMYDCWMSLDVEYATRLANAASEYKLKWI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHH
EEALPPDDYWGYAELRRNVPRGMLVTTGEHEATRWGFRMLLEMECCDILQPDVGWCGGIT
HHCCCCCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ELLKISALADAHGKLVVPHGSSVYSYHFVITRHNSPFSEFLMMAPKADEVVPMFNPMLLD
HHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCHHHHHEECCCCCCCCCCCCCEECC
EPVPVNGRMKASALDAPGFGVRLNPECALQRPFPR
CCCCCCCCEEECCCCCCCCCEEECCCHHCCCCCCC
>Mature Secondary Structure
MNNMPTIKHVRAFTVRGGGADYHDQGSGHWIDDHISTPMGRYPEYRQSRQSFGINVLGTL
CCCCCCCHHEEEEEEECCCCCCCCCCCCCEEHHHHCCCCCCCCHHHHHHHHCCCEEEEEE
VVEIEASDGTVGFSVTTGGELGCWIVEKHLARFIEGAKVTDIEKIWDQMFNATLYYGRKG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCC
IVLNTISGVDLALWDLLAKVRKEPVHALLGGPVRDELTFYATGARPDLAKKMGFIGGKLP
EEEEECCCCHHHHHHHHHHHHHCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCC
LHHGPAEREEGLKKNLELLGEMRQRVGDDFWLMYDCWMSLDVEYATRLANAASEYKLKWI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHHHHHHHHHHHHHHHHHHH
EEALPPDDYWGYAELRRNVPRGMLVTTGEHEATRWGFRMLLEMECCDILQPDVGWCGGIT
HHCCCCCCCCCHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ELLKISALADAHGKLVVPHGSSVYSYHFVITRHNSPFSEFLMMAPKADEVVPMFNPMLLD
HHHHHHHHHCCCCEEEEECCCCEEEEEEEEEECCCCHHHHHEECCCCCCCCCCCCCEECC
EPVPVNGRMKASALDAPGFGVRLNPECALQRPFPR
CCCCCCCCEEECCCCCCCCCEEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA