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The map label for this gene is gatA2 [H]

Identifier: 91787359

GI number: 91787359

Start: 1515148

End: 1516755

Strand: Reverse

Name: gatA2 [H]

Synonym: Bpro_1465

Alternate gene names: 91787359

Gene position: 1516755-1515148 (Counterclockwise)

Preceding gene: 91787360

Following gene: 91787357

Centisome position: 29.17

GC content: 68.97

Gene sequence:

>1608_bases
ATGACGGCCCCCACGCTCGGCACTGACGTGTCCTCGCTGCCCCCCGAGGGGGCGCATTTCGCCTTGGGGCGGCCCGGCGG
CGAAACAGCAGCCTCCACGCTCGTCGAAAAATCCGCCGTCGAACTGCGCCGCCTGATCGGCAGCCGCCAGCTCTCGCCGG
TGGAATTGCTCGACGCCTGCATTGCGCGCATTGAAAGCGTCAACCCCGCCATCAACGCGATCTGTGCAACCGACTTCACA
CGCGCACGCGAGACCGCGCGCCAGGCCGAAGCGCAGGTGATGCGCGGCGAGCCGCTGGGCCTGCTGCACGGTTTGCCCCT
GGGCGTGAAGGACCTGCAGGACACGGCGGGCCTGCTCACCACCTATGGCAATGTGCGGCTGCGCGGCAATGTGCCCAGCG
CCGACAACGCGCTGGTGGCCCGGCTGCGCGCCGCCGGCGCCATCGTTACCGCCAAGACCAACGTGCCCGACATGGGCGCG
GGCGCCAACACGCGCAACCCGGTCTGGGGCGCGACCGGCAACCCGTTCAACCCCGCGCTCAATGCCGGCGGTTCCTCGGG
CGGCTCGGCCGCGGCGCTGGCCGCCGACATGCTGCCGCTGTGCACCGGTTCGGACACCGGTGGTTCGCTGCGCATTCCGG
CGGCGCTGTGCGGCGTAGTGGGCCTGCGCCCCTCGCCCGGGCTGGTGGCCAACGATGCGCGGCCCCTGGGCTGGTCCGTC
ATTTCAGTGCTGGGGCCCATGGGGCGCACTGTGGCCGACACCGCGCTGATGTTTGCCGCCAGTGTGGGACCCGATGCGCG
GGACCCGCTGTCGTACCCGGTCGATGCCTCGGCATTCTGGCCGCTCCAGCAGCTTGATCTTTCAACGCTGCGCGTTGGCA
CAACGGAAGACTTCGGCCTGTGCATCGTCGACCCCGAAATCCGCCGCGTGTTCCGCCAGCGCGTCGAGGCGATCCGGCCG
CTGGTGGCGAGCTGCGAGCCCGTGGACCTGCAACTGGGCGACGCCGACCGCGCCTTTGACATCCTGCGCGCCGAAAGTTT
TGTCGCCGCCTTTGCCGAGACCTGGCAAAAAGAGCCCGAAACGCTGGGCCCTAACGTGCGCGCCAACATGGACATCGCGG
CAGCGATCACTCTGGCCGACCGAGCCTGGGCGCACCTGGAGCAGACCCGCATTGCGCGCCGCTTCGCCAGGGCCTTCGAG
CAATACGACCTGATCCTCGCACCAACCACGCCGGTCACACCCTTCCCGTGGACCGAGCTCTACGCCGGCAACATCGACGG
CCAGGCCATGCGCAACTACTACCACTGGCTGGGCCTGACCTATGTGGTCACGCTGGCCACCAATCCGGCGCTGTCACTGC
CCTGCGGCAGCGACGAGCACGGCATGCCGTTTGGCCTGCAAGTGATTGGGCGTTTGCATGGCGACGCGCAACTGCTGGCG
GCGGCCCAGGCGCTGGAGCAGGCGTTCGAAGCCACACCAGCGCTGCGCCGTCCGAAGCCGGCAATCGAGGCGCTGCGCAC
CGCGCGGCCCGAACTCAAGTCCATCGTCACGCACCCGCCCGAAACAGGCAGTTTGAGAGACTCCGGCGACGGCATGACAG
CTGTTTAG

Upstream 100 bases:

>100_bases
CCAGGCTACCGCGCCATTGCCGACTGGCCCGGCATGGTCACCGCCGACATCGCCCGCATGAAAGCCATTGCCGAGCGCTC
GCAAATCAAGGTGGACTGAT

Downstream 100 bases:

>100_bases
CCGGACAGGACCCACTCACCGGTCAACAGGTCCGTATCCGCGCCGTATCCGCGGCAAAACGGCCTCAAGGCAAAACGGGG
GTCGACAGAGGCAACTCAAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTAPTLGTDVSSLPPEGAHFALGRPGGETAASTLVEKSAVELRRLIGSRQLSPVELLDACIARIESVNPAINAICATDFT
RARETARQAEAQVMRGEPLGLLHGLPLGVKDLQDTAGLLTTYGNVRLRGNVPSADNALVARLRAAGAIVTAKTNVPDMGA
GANTRNPVWGATGNPFNPALNAGGSSGGSAAALAADMLPLCTGSDTGGSLRIPAALCGVVGLRPSPGLVANDARPLGWSV
ISVLGPMGRTVADTALMFAASVGPDARDPLSYPVDASAFWPLQQLDLSTLRVGTTEDFGLCIVDPEIRRVFRQRVEAIRP
LVASCEPVDLQLGDADRAFDILRAESFVAAFAETWQKEPETLGPNVRANMDIAAAITLADRAWAHLEQTRIARRFARAFE
QYDLILAPTTPVTPFPWTELYAGNIDGQAMRNYYHWLGLTYVVTLATNPALSLPCGSDEHGMPFGLQVIGRLHGDAQLLA
AAQALEQAFEATPALRRPKPAIEALRTARPELKSIVTHPPETGSLRDSGDGMTAV

Sequences:

>Translated_535_residues
MTAPTLGTDVSSLPPEGAHFALGRPGGETAASTLVEKSAVELRRLIGSRQLSPVELLDACIARIESVNPAINAICATDFT
RARETARQAEAQVMRGEPLGLLHGLPLGVKDLQDTAGLLTTYGNVRLRGNVPSADNALVARLRAAGAIVTAKTNVPDMGA
GANTRNPVWGATGNPFNPALNAGGSSGGSAAALAADMLPLCTGSDTGGSLRIPAALCGVVGLRPSPGLVANDARPLGWSV
ISVLGPMGRTVADTALMFAASVGPDARDPLSYPVDASAFWPLQQLDLSTLRVGTTEDFGLCIVDPEIRRVFRQRVEAIRP
LVASCEPVDLQLGDADRAFDILRAESFVAAFAETWQKEPETLGPNVRANMDIAAAITLADRAWAHLEQTRIARRFARAFE
QYDLILAPTTPVTPFPWTELYAGNIDGQAMRNYYHWLGLTYVVTLATNPALSLPCGSDEHGMPFGLQVIGRLHGDAQLLA
AAQALEQAFEATPALRRPKPAIEALRTARPELKSIVTHPPETGSLRDSGDGMTAV
>Mature_534_residues
TAPTLGTDVSSLPPEGAHFALGRPGGETAASTLVEKSAVELRRLIGSRQLSPVELLDACIARIESVNPAINAICATDFTR
ARETARQAEAQVMRGEPLGLLHGLPLGVKDLQDTAGLLTTYGNVRLRGNVPSADNALVARLRAAGAIVTAKTNVPDMGAG
ANTRNPVWGATGNPFNPALNAGGSSGGSAAALAADMLPLCTGSDTGGSLRIPAALCGVVGLRPSPGLVANDARPLGWSVI
SVLGPMGRTVADTALMFAASVGPDARDPLSYPVDASAFWPLQQLDLSTLRVGTTEDFGLCIVDPEIRRVFRQRVEAIRPL
VASCEPVDLQLGDADRAFDILRAESFVAAFAETWQKEPETLGPNVRANMDIAAAITLADRAWAHLEQTRIARRFARAFEQ
YDLILAPTTPVTPFPWTELYAGNIDGQAMRNYYHWLGLTYVVTLATNPALSLPCGSDEHGMPFGLQVIGRLHGDAQLLAA
AQALEQAFEATPALRRPKPAIEALRTARPELKSIVTHPPETGSLRDSGDGMTAV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=25.5445544554455, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI195972892, Length=258, Percent_Identity=31.7829457364341, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI166795287, Length=285, Percent_Identity=30.1754385964912, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=506, Percent_Identity=27.6679841897233, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=29.7385620915033, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=469, Percent_Identity=25.5863539445629, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=329, Percent_Identity=27.0516717325228, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=243, Percent_Identity=30.8641975308642, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=320, Percent_Identity=28.75, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=320, Percent_Identity=28.75, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=250, Percent_Identity=31.2, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=392, Percent_Identity=28.3163265306122, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=241, Percent_Identity=26.5560165975104, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=471, Percent_Identity=21.8683651804671, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356731, Length=493, Percent_Identity=28.3975659229209, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24648113, Length=461, Percent_Identity=27.114967462039, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24648435, Length=502, Percent_Identity=25.4980079681275, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648437, Length=502, Percent_Identity=25.4980079681275, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI45550774, Length=502, Percent_Identity=25.4980079681275, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24652985, Length=503, Percent_Identity=26.2425447316103, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19922090, Length=503, Percent_Identity=26.2425447316103, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24652981, Length=503, Percent_Identity=26.2425447316103, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24652983, Length=503, Percent_Identity=26.2425447316103, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=25.6972111553785, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078093, Length=508, Percent_Identity=27.755905511811, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24648441, Length=430, Percent_Identity=23.7209302325581, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648439, Length=430, Percent_Identity=23.7209302325581, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 56474; Mature: 56343

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPTLGTDVSSLPPEGAHFALGRPGGETAASTLVEKSAVELRRLIGSRQLSPVELLDAC
CCCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IARIESVNPAINAICATDFTRARETARQAEAQVMRGEPLGLLHGLPLGVKDLQDTAGLLT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHE
TYGNVRLRGNVPSADNALVARLRAAGAIVTAKTNVPDMGAGANTRNPVWGATGNPFNPAL
ECCCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
NAGGSSGGSAAALAADMLPLCTGSDTGGSLRIPAALCGVVGLRPSPGLVANDARPLGWSV
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCEEECCCCCCHHHH
ISVLGPMGRTVADTALMFAASVGPDARDPLSYPVDASAFWPLQQLDLSTLRVGTTEDFGL
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHEEECCCCCCCE
CIVDPEIRRVFRQRVEAIRPLVASCEPVDLQLGDADRAFDILRAESFVAAFAETWQKEPE
EEECHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCH
TLGPNVRANMDIAAAITLADRAWAHLEQTRIARRFARAFEQYDLILAPTTPVTPFPWTEL
HCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
YAGNIDGQAMRNYYHWLGLTYVVTLATNPALSLPCGSDEHGMPFGLQVIGRLHGDAQLLA
EECCCCHHHHHHHHHHHHHHHEEEEECCCCEECCCCCCCCCCCHHHHHHHHHCCHHHHHH
AAQALEQAFEATPALRRPKPAIEALRTARPELKSIVTHPPETGSLRDSGDGMTAV
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAPTLGTDVSSLPPEGAHFALGRPGGETAASTLVEKSAVELRRLIGSRQLSPVELLDAC
CCCCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IARIESVNPAINAICATDFTRARETARQAEAQVMRGEPLGLLHGLPLGVKDLQDTAGLLT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHE
TYGNVRLRGNVPSADNALVARLRAAGAIVTAKTNVPDMGAGANTRNPVWGATGNPFNPAL
ECCCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
NAGGSSGGSAAALAADMLPLCTGSDTGGSLRIPAALCGVVGLRPSPGLVANDARPLGWSV
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCEEECCCCCCHHHH
ISVLGPMGRTVADTALMFAASVGPDARDPLSYPVDASAFWPLQQLDLSTLRVGTTEDFGL
HHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHEEECCCCCCCE
CIVDPEIRRVFRQRVEAIRPLVASCEPVDLQLGDADRAFDILRAESFVAAFAETWQKEPE
EEECHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCH
TLGPNVRANMDIAAAITLADRAWAHLEQTRIARRFARAFEQYDLILAPTTPVTPFPWTEL
HCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
YAGNIDGQAMRNYYHWLGLTYVVTLATNPALSLPCGSDEHGMPFGLQVIGRLHGDAQLLA
EECCCCHHHHHHHHHHHHHHHEEEEECCCCEECCCCCCCCCCCHHHHHHHHHCCHHHHHH
AAQALEQAFEATPALRRPKPAIEALRTARPELKSIVTHPPETGSLRDSGDGMTAV
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]