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The map label for this gene is ipk

Identifier: 91787190

GI number: 91787190

Start: 1336334

End: 1337263

Strand: Reverse

Name: ipk

Synonym: Bpro_1294

Alternate gene names: 91787190

Gene position: 1337263-1336334 (Counterclockwise)

Preceding gene: 91787191

Following gene: 91787189

Centisome position: 25.72

GC content: 62.8

Gene sequence:

>930_bases
ATGACACGCACTGCCAGCGCTCCTCGTTCCGTGGGTTCAGCACGCCCACTCAAAGCCATTTACGACGTTCCCGCCCCGGC
CAAGCTCAACCTGTTTTTGCACATCACGGGCCGGCGGGCCGATGGCTACCACCTGCTGCAGTCGGCCTTCATGCTGATCG
ACTGGTGCGACACCCTGCACTTTGAACTGCGCACCGATGGGCAGATCAGCCGCGCCGACCTGAGCGATCCAGGCAGTCCC
GGCTGCGGCGCAGCAGAACCCCTGCCAGCAGAAGATCTTGCCGTGCGGGCGGCCAGGGCCCTGCAGGCAGCGTGCGGCAC
CTCACTGGGTGTTCACATCGGTCTGGAAAAACGCATACCGTCTCAGGCGGGCATGGGCGGCGGCTCGTCCGACGCGGCGA
GCTGCCTGCTGGCACTGCAACGCCTCTGGGGCGTAACGTTGCCTGCCACGGAGTTGCGCGCGCTGGCCCTGTCGCTGGGC
GCGGACGTGCCTTTTTTCCTCTCGGGCGGGCATGCCTGGGTCGAAGGGATCGGCGAGAAAATCACCCCCATCGCGCTGCC
GGCGGCGCGGTTTGTGGTGGTCAAGCCTTCGGCCGGGCTCTCTACGCCCGCTATTTTCAGTGCGCCGGAACTCAAACGCG
ATACAGAAACTGCTACAATCCAAGGCTTTGCTGCAAATGCAGAAGGTCAGGTCTTCGGATTTGGCCGCAACGACTTGCAG
CCGGTCGCCCAGGCACTGTGTCCGCCGATTGGTCAATCACTCGATTGGCTGTCAGCGCAACACCTTCAGGGACGAATGAC
CGGCTCGGGAAGTGCTGTGTTTGCGCTACTGCCGCACGATGTGGATCTGTCAGATGTACCAGGAACTCCTGGTGACTGGA
AAATCCGCAAGTGCAGCAATTTGCAAGCGCATCCCCTGGCCGGTTGGTAA

Upstream 100 bases:

>100_bases
CACCACCAGCCGATCTTCGTGTCGTGCTGGATCAGTAAGGTCCGGTGCAGCGCCCTCCGCCGCTTTAATGGATTAACGGA
TTCATCGGAATTTTTCAGAC

Downstream 100 bases:

>100_bases
TAAAATAACGGCTCTTGGGAATGTGTTTTCAAGCGACCATGAGTGAACAGGTTATAGGTCGGTTGTTGGCGAGTTGCGTT
GTCTGGTTTCAGGCTACGCT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTRTASAPRSVGSARPLKAIYDVPAPAKLNLFLHITGRRADGYHLLQSAFMLIDWCDTLHFELRTDGQISRADLSDPGSP
GCGAAEPLPAEDLAVRAARALQAACGTSLGVHIGLEKRIPSQAGMGGGSSDAASCLLALQRLWGVTLPATELRALALSLG
ADVPFFLSGGHAWVEGIGEKITPIALPAARFVVVKPSAGLSTPAIFSAPELKRDTETATIQGFAANAEGQVFGFGRNDLQ
PVAQALCPPIGQSLDWLSAQHLQGRMTGSGSAVFALLPHDVDLSDVPGTPGDWKIRKCSNLQAHPLAGW

Sequences:

>Translated_309_residues
MTRTASAPRSVGSARPLKAIYDVPAPAKLNLFLHITGRRADGYHLLQSAFMLIDWCDTLHFELRTDGQISRADLSDPGSP
GCGAAEPLPAEDLAVRAARALQAACGTSLGVHIGLEKRIPSQAGMGGGSSDAASCLLALQRLWGVTLPATELRALALSLG
ADVPFFLSGGHAWVEGIGEKITPIALPAARFVVVKPSAGLSTPAIFSAPELKRDTETATIQGFAANAEGQVFGFGRNDLQ
PVAQALCPPIGQSLDWLSAQHLQGRMTGSGSAVFALLPHDVDLSDVPGTPGDWKIRKCSNLQAHPLAGW
>Mature_308_residues
TRTASAPRSVGSARPLKAIYDVPAPAKLNLFLHITGRRADGYHLLQSAFMLIDWCDTLHFELRTDGQISRADLSDPGSPG
CGAAEPLPAEDLAVRAARALQAACGTSLGVHIGLEKRIPSQAGMGGGSSDAASCLLALQRLWGVTLPATELRALALSLGA
DVPFFLSGGHAWVEGIGEKITPIALPAARFVVVKPSAGLSTPAIFSAPELKRDTETATIQGFAANAEGQVFGFGRNDLQP
VAQALCPPIGQSLDWLSAQHLQGRMTGSGSAVFALLPHDVDLSDVPGTPGDWKIRKCSNLQAHPLAGW

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=276, Percent_Identity=38.0434782608696, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_POLSJ (Q12DZ8)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548142.1
- ProteinModelPortal:   Q12DZ8
- SMR:   Q12DZ8
- STRING:   Q12DZ8
- GeneID:   4012188
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1294
- NMPDR:   fig|296591.1.peg.1968
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   NDPITIA
- PhylomeDB:   Q12DZ8
- ProtClustDB:   PRK00343
- BioCyc:   PSP296591:BPRO_1294-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32336; Mature: 32205

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: ACT_SITE 28-28 ACT_SITE 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTASAPRSVGSARPLKAIYDVPAPAKLNLFLHITGRRADGYHLLQSAFMLIDWCDTLH
CCCCCCCCHHCCCCCCCHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEE
FELRTDGQISRADLSDPGSPGCGAAEPLPAEDLAVRAARALQAACGTSLGVHIGLEKRIP
EEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCC
SQAGMGGGSSDAASCLLALQRLWGVTLPATELRALALSLGADVPFFLSGGHAWVEGIGEK
HHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCC
ITPIALPAARFVVVKPSAGLSTPAIFSAPELKRDTETATIQGFAANAEGQVFGFGRNDLQ
CCCEEECCEEEEEECCCCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCCHH
PVAQALCPPIGQSLDWLSAQHLQGRMTGSGSAVFALLPHDVDLSDVPGTPGDWKIRKCSN
HHHHHHCCCCCCCHHHHHHHHHCCEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCC
LQAHPLAGW
CCCCCCCCC
>Mature Secondary Structure 
TRTASAPRSVGSARPLKAIYDVPAPAKLNLFLHITGRRADGYHLLQSAFMLIDWCDTLH
CCCCCCCHHCCCCCCCHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEE
FELRTDGQISRADLSDPGSPGCGAAEPLPAEDLAVRAARALQAACGTSLGVHIGLEKRIP
EEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCC
SQAGMGGGSSDAASCLLALQRLWGVTLPATELRALALSLGADVPFFLSGGHAWVEGIGEK
HHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCC
ITPIALPAARFVVVKPSAGLSTPAIFSAPELKRDTETATIQGFAANAEGQVFGFGRNDLQ
CCCEEECCEEEEEECCCCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCEEECCCCCHH
PVAQALCPPIGQSLDWLSAQHLQGRMTGSGSAVFALLPHDVDLSDVPGTPGDWKIRKCSN
HHHHHHCCCCCCCHHHHHHHHHCCEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCC
LQAHPLAGW
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA