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The map label for this gene is pth

Identifier: 91787187

GI number: 91787187

Start: 1333355

End: 1333969

Strand: Reverse

Name: pth

Synonym: Bpro_1291

Alternate gene names: 91787187

Gene position: 1333969-1333355 (Counterclockwise)

Preceding gene: 91787188

Following gene: 91787186

Centisome position: 25.65

GC content: 61.79

Gene sequence:

>615_bases
ATGATCAAACTTTTTGTTGGCCTGGGCAACCCGGGTGCCGAATACGAGGCGACCCGCCACAACGCCGGCTTCTGGTGGAT
AGACGCGCTCTCGCGCGAATTGCAGGCGCCGCTCACCCTGGACAAGAGTTATCACGGACAGGTCGCGCGCACCACCCTGA
ACGGCAAAACCGTATGGCTTCTCAAGCCCCTGACCTTCATGAATGTCTCGGGCAAATCCGTGGCGGCGCTCGCCCGGTTT
TTCAAGATCGCGCCCGACGAGGTGCTGGTGGCCCACGACGAACTCGACATCGTGCCCGGACAGGTCAAACTCAAGTTTGG
CGGCAGCCATGCGGGCCACAACGGCCTGCGCGACATTCATGCCCAGCTCGGCACCGACGACTACTGGCGCCTGCGCATCG
GCGTAGGCCATCCCGGCGTGAAAGCCGAGGTCATCAACTGGGTGCTGAAAAAGCCGTCACAGGAACATCGCGTAGCGATT
GAAGAATGCATTGACCGCGCACTCAAGGCGGTACCGGCCCTGCTGGCAGGCGAGATGGAAAAAGCCACCATGCTGATTCA
CACCAGCCAGCCACCGCGTCCCAAGCCACCGCGCAGGACGGAACCGGAAGACTGA

Upstream 100 bases:

>100_bases
CCTCCCCGGAGGCCAAGGGCCGTTTTTCTTGAGGACCACGTTCTGCTGCACCGATAATCCCGGTTGCGGGACGCACCGCT
TTCTCTCCCTTACACACAGC

Downstream 100 bases:

>100_bases
TCGCAGCAGCCAGTTAGGCTCAGGAAGGAACGCGCCCACCGTCTGTCTGCGCAACAAGACTGAATTCAGGTTCAATCTCC
GTCCTGTGGATCTCTGCAAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIKLFVGLGNPGAEYEATRHNAGFWWIDALSRELQAPLTLDKSYHGQVARTTLNGKTVWLLKPLTFMNVSGKSVAALARF
FKIAPDEVLVAHDELDIVPGQVKLKFGGSHAGHNGLRDIHAQLGTDDYWRLRIGVGHPGVKAEVINWVLKKPSQEHRVAI
EECIDRALKAVPALLAGEMEKATMLIHTSQPPRPKPPRRTEPED

Sequences:

>Translated_204_residues
MIKLFVGLGNPGAEYEATRHNAGFWWIDALSRELQAPLTLDKSYHGQVARTTLNGKTVWLLKPLTFMNVSGKSVAALARF
FKIAPDEVLVAHDELDIVPGQVKLKFGGSHAGHNGLRDIHAQLGTDDYWRLRIGVGHPGVKAEVINWVLKKPSQEHRVAI
EECIDRALKAVPALLAGEMEKATMLIHTSQPPRPKPPRRTEPED
>Mature_204_residues
MIKLFVGLGNPGAEYEATRHNAGFWWIDALSRELQAPLTLDKSYHGQVARTTLNGKTVWLLKPLTFMNVSGKSVAALARF
FKIAPDEVLVAHDELDIVPGQVKLKFGGSHAGHNGLRDIHAQLGTDDYWRLRIGVGHPGVKAEVINWVLKKPSQEHRVAI
EECIDRALKAVPALLAGEMEKATMLIHTSQPPRPKPPRRTEPED

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=166, Percent_Identity=36.144578313253, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1787455, Length=187, Percent_Identity=53.475935828877, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_POLSJ (Q12E01)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548139.1
- ProteinModelPortal:   Q12E01
- SMR:   Q12E01
- STRING:   Q12E01
- GeneID:   4012184
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1291
- NMPDR:   fig|296591.1.peg.1971
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   SHAGHNG
- PhylomeDB:   Q12E01
- ProtClustDB:   PRK05426
- BioCyc:   PSP296591:BPRO_1291-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 22615; Mature: 22615

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLFVGLGNPGAEYEATRHNAGFWWIDALSRELQAPLTLDKSYHGQVARTTLNGKTVWL
CEEEEEEECCCCCCCEECCCCCCEEEHHHHHHHHCCCEEECCCCCCCEEEEEECCCEEEE
LKPLTFMNVSGKSVAALARFFKIAPDEVLVAHDELDIVPGQVKLKFGGSHAGHNGLRDIH
ECCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCEECCCEEEEEECCCCCCCCCHHHHH
AQLGTDDYWRLRIGVGHPGVKAEVINWVLKKPSQEHRVAIEECIDRALKAVPALLAGEME
HHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
KATMLIHTSQPPRPKPPRRTEPED
CEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIKLFVGLGNPGAEYEATRHNAGFWWIDALSRELQAPLTLDKSYHGQVARTTLNGKTVWL
CEEEEEEECCCCCCCEECCCCCCEEEHHHHHHHHCCCEEECCCCCCCEEEEEECCCEEEE
LKPLTFMNVSGKSVAALARFFKIAPDEVLVAHDELDIVPGQVKLKFGGSHAGHNGLRDIH
ECCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCEECCCEEEEEECCCCCCCCCHHHHH
AQLGTDDYWRLRIGVGHPGVKAEVINWVLKKPSQEHRVAIEECIDRALKAVPALLAGEME
HHCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
KATMLIHTSQPPRPKPPRRTEPED
CEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA