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The map label for this gene is yiaO [H]

Identifier: 91787186

GI number: 91787186

Start: 1331688

End: 1332683

Strand: Reverse

Name: yiaO [H]

Synonym: Bpro_1290

Alternate gene names: 91787186

Gene position: 1332683-1331688 (Counterclockwise)

Preceding gene: 91787187

Following gene: 91787185

Centisome position: 25.63

GC content: 58.13

Gene sequence:

>996_bases
ATGAGCAAGATCGGATTCATGCTGCTTACCTGGGCGCTGGCCTTTGCCGCTCAGACGGCCATCGCCAAAACCACCTTCAA
TCTCTCCACGCCCGACCCGGATAGTTCTGAAATTACCCTGGCAGCCAAAAAATTCGCGGAAATTGTCGCGGCCAAAAGTG
GCGGGCAAATCGAAGTCAAGGTGTTTCCGAACGGGCAGCTTTACGGGGGTGATCCTTCCGCTGCCGTGCGCCAACTGGCG
AGCGGGTCCTTAGATATGCTGCTGCTGTCAAGCTCGCTGTATGCCAACTTCAATCCGAAGTTCACCGCCATCTCCGTCCC
GTACCTGTTTGATGACAATGCCCAGCTTCGTGCTTATCTGGCAGGCCCCCTGGGGCAGGAGTTGCTCACTGACCTGAACG
GCATAGGCATCAAGGGGTTGGCGCTCTGGCAGCGTCCGTTCCGTCAGATGACCAATTCGCGCCGGGCGATCAACCAGCCG
CAGGATCTGGCCGGGATGAAGTTCCGTGTCCCGAATAACCCCCTGTGGATGGAGTTTTTTGGCAAGCTCGGCGCCGTTCC
GACCCCCATGGCATTCGGCGAGGTCTACAACGCCCTGCAGCTGAAGGTGGTCGATGGCCAGGAAAACCCGATCAACATTC
CGGTAACCGCCAAGTTTTACGAAGTCCAGAAGTTCGCCACGCTGACCAACCACATGGCCGACGGTTGGGTGCTGGCCATC
AATCCCGCCAAATTCGCGGCGCTCCCGGAGGCGGAGAAGCAGGCGCTGCAAGCTGCCAGCGTAGAGGCGGAAGCATGGAA
GTCTGCCAATGACGCAGCCGATGCCAGCCGATCGGTGGAATTCCTGCGCTCCAAGGGCGTGCAGGTCAATAGCCTGAGCC
CGGAGCAACAAAAAGCCTTTGTGGCGGTCTCCAAAGAACTGTTCCCCCGCTTCTCAACGCTTGTCAAGGATCAGGCCTTC
TTTGACAAGACCTTGTCCTTTGTCGGCAAAAAGTGA

Upstream 100 bases:

>100_bases
CAATTCTTTCCCGGGGGTTTCTACTAATTGGATAGACCAATTATTGTTCATACACTCAAATCTCATGAAAATTGGTATAA
CCATTAACAGGAGACACGTT

Downstream 100 bases:

>100_bases
GCAGCAACCGGCCCATATCTTCCAAACCAGTCTGGTATCCCGGGTCATGAGCATCCAGGATTCCAGTCGGCGAAAAAAGG
AACATTCCGTCATGTCAACC

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSKIGFMLLTWALAFAAQTAIAKTTFNLSTPDPDSSEITLAAKKFAEIVAAKSGGQIEVKVFPNGQLYGGDPSAAVRQLA
SGSLDMLLLSSSLYANFNPKFTAISVPYLFDDNAQLRAYLAGPLGQELLTDLNGIGIKGLALWQRPFRQMTNSRRAINQP
QDLAGMKFRVPNNPLWMEFFGKLGAVPTPMAFGEVYNALQLKVVDGQENPINIPVTAKFYEVQKFATLTNHMADGWVLAI
NPAKFAALPEAEKQALQAASVEAEAWKSANDAADASRSVEFLRSKGVQVNSLSPEQQKAFVAVSKELFPRFSTLVKDQAF
FDKTLSFVGKK

Sequences:

>Translated_331_residues
MSKIGFMLLTWALAFAAQTAIAKTTFNLSTPDPDSSEITLAAKKFAEIVAAKSGGQIEVKVFPNGQLYGGDPSAAVRQLA
SGSLDMLLLSSSLYANFNPKFTAISVPYLFDDNAQLRAYLAGPLGQELLTDLNGIGIKGLALWQRPFRQMTNSRRAINQP
QDLAGMKFRVPNNPLWMEFFGKLGAVPTPMAFGEVYNALQLKVVDGQENPINIPVTAKFYEVQKFATLTNHMADGWVLAI
NPAKFAALPEAEKQALQAASVEAEAWKSANDAADASRSVEFLRSKGVQVNSLSPEQQKAFVAVSKELFPRFSTLVKDQAF
FDKTLSFVGKK
>Mature_330_residues
SKIGFMLLTWALAFAAQTAIAKTTFNLSTPDPDSSEITLAAKKFAEIVAAKSGGQIEVKVFPNGQLYGGDPSAAVRQLAS
GSLDMLLLSSSLYANFNPKFTAISVPYLFDDNAQLRAYLAGPLGQELLTDLNGIGIKGLALWQRPFRQMTNSRRAINQPQ
DLAGMKFRVPNNPLWMEFFGKLGAVPTPMAFGEVYNALQLKVVDGQENPINIPVTAKFYEVQKFATLTNHMADGWVLAIN
PAKFAALPEAEKQALQAASVEAEAWKSANDAADASRSVEFLRSKGVQVNSLSPEQQKAFVAVSKELFPRFSTLVKDQAFF
DKTLSFVGKK

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=299, Percent_Identity=35.1170568561873, Blast_Score=184, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36037; Mature: 35905

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIGFMLLTWALAFAAQTAIAKTTFNLSTPDPDSSEITLAAKKFAEIVAAKSGGQIEVK
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEE
VFPNGQLYGGDPSAAVRQLASGSLDMLLLSSSLYANFNPKFTAISVPYLFDDNAQLRAYL
ECCCCEEECCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCEEEEEECEEECCCCCEEEEE
AGPLGQELLTDLNGIGIKGLALWQRPFRQMTNSRRAINQPQDLAGMKFRVPNNPLWMEFF
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCHHHHHH
GKLGAVPTPMAFGEVYNALQLKVVDGQENPINIPVTAKFYEVQKFATLTNHMADGWVLAI
HHHCCCCCCHHHHHHHCEEEEEEECCCCCCEECEEEEHHHHHHHHHHHHHHCCCCEEEEE
NPAKFAALPEAEKQALQAASVEAEAWKSANDAADASRSVEFLRSKGVQVNSLSPEQQKAF
CCHHHHCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHH
VAVSKELFPRFSTLVKDQAFFDKTLSFVGKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKIGFMLLTWALAFAAQTAIAKTTFNLSTPDPDSSEITLAAKKFAEIVAAKSGGQIEVK
CCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEE
VFPNGQLYGGDPSAAVRQLASGSLDMLLLSSSLYANFNPKFTAISVPYLFDDNAQLRAYL
ECCCCEEECCCHHHHHHHHHCCCCEEEEEEHHHHCCCCCCEEEEEECEEECCCCCEEEEE
AGPLGQELLTDLNGIGIKGLALWQRPFRQMTNSRRAINQPQDLAGMKFRVPNNPLWMEFF
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCHHHHHH
GKLGAVPTPMAFGEVYNALQLKVVDGQENPINIPVTAKFYEVQKFATLTNHMADGWVLAI
HHHCCCCCCHHHHHHHCEEEEEEECCCCCCEECEEEEHHHHHHHHHHHHHHCCCCEEEEE
NPAKFAALPEAEKQALQAASVEAEAWKSANDAADASRSVEFLRSKGVQVNSLSPEQQKAF
CCHHHHCCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHHHH
VAVSKELFPRFSTLVKDQAFFDKTLSFVGKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]