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The map label for this gene is miaB [H]

Identifier: 91787018

GI number: 91787018

Start: 1157199

End: 1158542

Strand: Reverse

Name: miaB [H]

Synonym: Bpro_1120

Alternate gene names: 91787018

Gene position: 1158542-1157199 (Counterclockwise)

Preceding gene: 91787019

Following gene: 91787017

Centisome position: 22.28

GC content: 62.43

Gene sequence:

>1344_bases
ATGAGCAAAAAAGTTTTTATCAAAACCTTCGGGTGCCAGATGAACGAGTACGACTCGGACAAGATGTCCGACGTACTGCA
TGCGGCAGAGGGCTACGAGCCCACCCAGAATGTCGACGAAGCCGACCTGATCCTGTTCAACACCTGCTCGGTGCGTGAAA
AGGCGCAGGAAAAAGTCTTCAGTGACCTGGGCCGCGTCAAGCACCTGAAGGAAAAAGGCGTGCTCATCGGCGTGGGCGGT
TGCGTGGCCAGCCAGGAAGGCGCGGCCATCATCCAGCGCGCGCCTTATGTTGACGTGGTGTTCGGTCCCCAGACGCTGCA
TCGCCTGCCGGACCTGCTGGCCAAACGCGCGCGACTGAACAAACCCCAGGTCGACATCAGCTTTCCCGAAATCGAGAAGT
TCGATCACCTGCCGCCGGCCCGCGTGGAAGGTGCCAGCGCCTTTGTCAGCATCATGGAGGGCTGCTCCAAGTACTGCAGC
TACTGCGTCGTGCCGTACACGCGCGGCGAAGAGGTCTCTCGGCCGTTTGACGACGTGCTGGTCGAAGTGGCCGGCCTAGC
CGACCAGGGTGTGAAAGAAGTGACGCTGCTGGGGCAAAACGTCAACGCCTACCGCGGCCCCATGGGTGACACGGCCGAGA
TCGCCGATTTCGCCACGCTGCTCGAATACGTGTCTGACATCCCCGGCATCGAGCGCATCCGCTACGTCACCAGCCACCCC
AACGAGTTCACGCAAAGCCTGATCGACGCCTATGCCCGGCTGCCCAAGCTGGTCAACCACCTGCACCTGCCGGTGCAGCA
CGGCTCGGACCGGATACTGATGGCCATGAAGCGCGGCTACACCGCGATGGAATACAAAAGCACCATCCGCAAGCTGCGAG
CCATCCGCCCGGACCTGGCGATGAGCAGCGATTTCATTGTGGGCTTTCCTGGCGAAACCGAAGACGACTTCAACAAGATG
ATGAAGCTGATCGACGATGTTGGCTACGACACCTCCTTCAGTTTCATCTACAGCCCGCGGCCGGGCACGCCCGCGGCCGC
CCTGCACGACGATACGCCGCACGAGGTCAAGCTCAGGCGCCTGCAGCATTTGCAGGCCACGATTGACGACAGCGTGCGCG
CCATCAGCGCCAGTCGCCTGGGCACGGTGCAGCGCATCCTGGTGGAAGGCGCGTCGCGCAAGGACCCGACCGAGCTGATG
GGCCGCACCGAGTGCAACCGGGTGGTCAATTTCAAGGGCCAGCCGCGGCTGATCGGCCAGATGGTCGACATCACCATCAC
ACAGGCGACACAGCGCTCGCTGCGCGGCGAAGTCCTGACGCGGGACGTCGCCGTGCAGGCCTGA

Upstream 100 bases:

>100_bases
GTAATCCGGCGAAATCCCTCGCCAGACAAGCCTTTCGCCCCATTCGGCCCTTTCGCCTTTCATGCAAGGGCGAATAAAGC
TATCACTTATATAGCAAATC

Downstream 100 bases:

>100_bases
GGCAACGGGTTGCGCCAATACACCACGCACATGCTGCAGCGATTTTCGTTTCGGCAACTGCTTGTCATCGCGTTCCTGCT
GATCGCCGCCTTGCTGGGCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVFSDLGRVKHLKEKGVLIGVGG
CVASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLNKPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCS
YCVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHP
NEFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAMSSDFIVGFPGETEDDFNKM
MKLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRRLQHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELM
GRTECNRVVNFKGQPRLIGQMVDITITQATQRSLRGEVLTRDVAVQA

Sequences:

>Translated_447_residues
MSKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVFSDLGRVKHLKEKGVLIGVGG
CVASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLNKPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCS
YCVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHP
NEFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAMSSDFIVGFPGETEDDFNKM
MKLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRRLQHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELM
GRTECNRVVNFKGQPRLIGQMVDITITQATQRSLRGEVLTRDVAVQA
>Mature_446_residues
SKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVFSDLGRVKHLKEKGVLIGVGGC
VASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLNKPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSY
CVVPYTRGEEVSRPFDDVLVEVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHPN
EFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAMSSDFIVGFPGETEDDFNKMM
KLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRRLQHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELMG
RTECNRVVNFKGQPRLIGQMVDITITQATQRSLRGEVLTRDVAVQA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=487, Percent_Identity=36.9609856262834, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI28872782, Length=487, Percent_Identity=36.9609856262834, Blast_Score=300, Evalue=3e-81,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=28.7912087912088, Blast_Score=171, Evalue=2e-42,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=63.4920634920635, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI1787057, Length=459, Percent_Identity=30.718954248366, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=35, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=324, Percent_Identity=27.7777777777778, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=33.9491916859122, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI19922432, Length=448, Percent_Identity=28.5714285714286, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49710; Mature: 49579

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
SDLGRVKHLKEKGVLIGVGGCVASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLN
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCC
KPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVL
CCCEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHHH
VEVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHP
HHHHHHHHCCCCEEHEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
NEFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCC
MSSDFIVGFPGETEDDFNKMMKLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRR
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
LQHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELMGRTECNRVVNFKGQPRLIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHCCCCCCCHHHHH
MVDITITQATQRSLRGEVLTRDVAVQA
HHHHEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
SDLGRVKHLKEKGVLIGVGGCVASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLN
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCC
KPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVL
CCCEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHCCCHHHHH
VEVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHP
HHHHHHHHCCCCEEHEECCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
NEFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCC
MSSDFIVGFPGETEDDFNKMMKLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRR
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHH
LQHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELMGRTECNRVVNFKGQPRLIGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHCCCCCCCHHHHH
MVDITITQATQRSLRGEVLTRDVAVQA
HHHHEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA