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The map label for this gene is Dml [H]

Identifier: 91786928

GI number: 91786928

Start: 1055782

End: 1056645

Strand: Reverse

Name: Dml [H]

Synonym: Bpro_1029

Alternate gene names: 91786928

Gene position: 1056645-1055782 (Counterclockwise)

Preceding gene: 91786929

Following gene: 91786927

Centisome position: 20.32

GC content: 63.43

Gene sequence:

>864_bases
ATGACACCCGCCCAACAACTCCAGCAACTGCTCAAGACCGGCAACATCGTGATGGCGCCGGGCGCACCCGATGCCATCAC
CGCACGCCTGATCCAGAAGGCAGGCTTTCCGGCCATCTACATGACGGGTTTCGGCGCAACCGCCAGCCGCCTGGGCACGC
CCGACATCGGCCTGCTGAGCCAGACCGAGATGACCACCCATGCACGCGACATGGCCCGGGTGGTTGACATCCCCATCATT
GCCGATGCCGACACCGGCTATGGTGGGCCGTCCAACATCCACCGCACGGTACGCGAGTACATCCAGGCCGGCGTGGCCGC
CATCCACCTGGAAGACCAGGTCGCGCCCAAGCGCTGCGGCCAGATGGCCGGCATCCGGCTGATGGATGCGCAAGAAAACG
TGTTGCGACTGAAATGCGCCATCGAGGCGCGCGGTCGGGATGAGCTGCTCATCATCGGCCGTACCGATGCCCTGCCCGCG
GCCGGCATTGATGAAGCCATCCGGCGCGCCCATCTGTATCAGGAGGCCGGCGTGGACTTGGTCTTCGTCGACGGCATCAA
AAAAATCGCCGAAGTCGAGGCCGTTGCCAGGGCGGTCCAGGGACCCAAGGTCGTCTCCATCGTGGACGGCAATGAGACCA
CGGCACTGACAGCCAAGGACCTGCAGGACATGGGTTTTTCAGTGGTGTTTTATGCGGTGACAGCGCTTTTCACCGCAGTG
AAAGCCGTCTCGGATGCGCTCGCCGAACTGCAGCGTGCCGGCACACCCAAGGCGAGCGAACGCGCCATGGTGAGCTATGC
CGAATTCAGCGCGCTGGTGGACCTGGATTTCCACAAAGACCTGGATGACCGGTTTGGCGCCTGA

Upstream 100 bases:

>100_bases
GAAGCCTTTCGCCTGGCCAGTGCTGCGGCGCTGAAAGCCGCCCCCGCGCCTATCAAACTGAACGTCGCAGGCACCTGACC
CGACTCTTTTAAAAACCACC

Downstream 100 bases:

>100_bases
GACAGGCACGCGGCCCAACGGGGTGACGCCACATGTCGACCCCACCTGCCGCTACTGCACGGCGACGGTGGCGGAACTTG
AGGACGTGATGTTCCATGTC

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTPAQQLQQLLKTGNIVMAPGAPDAITARLIQKAGFPAIYMTGFGATASRLGTPDIGLLSQTEMTTHARDMARVVDIPII
ADADTGYGGPSNIHRTVREYIQAGVAAIHLEDQVAPKRCGQMAGIRLMDAQENVLRLKCAIEARGRDELLIIGRTDALPA
AGIDEAIRRAHLYQEAGVDLVFVDGIKKIAEVEAVARAVQGPKVVSIVDGNETTALTAKDLQDMGFSVVFYAVTALFTAV
KAVSDALAELQRAGTPKASERAMVSYAEFSALVDLDFHKDLDDRFGA

Sequences:

>Translated_287_residues
MTPAQQLQQLLKTGNIVMAPGAPDAITARLIQKAGFPAIYMTGFGATASRLGTPDIGLLSQTEMTTHARDMARVVDIPII
ADADTGYGGPSNIHRTVREYIQAGVAAIHLEDQVAPKRCGQMAGIRLMDAQENVLRLKCAIEARGRDELLIIGRTDALPA
AGIDEAIRRAHLYQEAGVDLVFVDGIKKIAEVEAVARAVQGPKVVSIVDGNETTALTAKDLQDMGFSVVFYAVTALFTAV
KAVSDALAELQRAGTPKASERAMVSYAEFSALVDLDFHKDLDDRFGA
>Mature_286_residues
TPAQQLQQLLKTGNIVMAPGAPDAITARLIQKAGFPAIYMTGFGATASRLGTPDIGLLSQTEMTTHARDMARVVDIPIIA
DADTGYGGPSNIHRTVREYIQAGVAAIHLEDQVAPKRCGQMAGIRLMDAQENVLRLKCAIEARGRDELLIIGRTDALPAA
GIDEAIRRAHLYQEAGVDLVFVDGIKKIAEVEAVARAVQGPKVVSIVDGNETTALTAKDLQDMGFSVVFYAVTALFTAVK
AVSDALAELQRAGTPKASERAMVSYAEFSALVDLDFHKDLDDRFGA

Specific function: Catalyzes the formation of proponate and pyruvate from (2R,3S)-2,3-dimethylmalate. Has no activity toward dimethylmaleate, malate, citramalate, isocitrate and citrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=286, Percent_Identity=32.1678321678322, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1790445, Length=91, Percent_Identity=42.8571428571429, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17561814, Length=187, Percent_Identity=31.0160427807487, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71982926, Length=187, Percent_Identity=31.0160427807487, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325263, Length=184, Percent_Identity=28.804347826087, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320908, Length=162, Percent_Identity=32.0987654320988, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.32 [H]

Molecular weight: Translated: 30648; Mature: 30516

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAQQLQQLLKTGNIVMAPGAPDAITARLIQKAGFPAIYMTGFGATASRLGTPDIGLLS
CCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCCCC
QTEMTTHARDMARVVDIPIIADADTGYGGPSNIHRTVREYIQAGVAAIHLEDQVAPKRCG
CHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
QMAGIRLMDAQENVLRLKCAIEARGRDELLIIGRTDALPAAGIDEAIRRAHLYQEAGVDL
HHHCEEEECCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCE
VFVDGIKKIAEVEAVARAVQGPKVVSIVDGNETTALTAKDLQDMGFSVVFYAVTALFTAV
EEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHH
KAVSDALAELQRAGTPKASERAMVSYAEFSALVDLDFHKDLDDRFGA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECHHCCCCHHHCCC
>Mature Secondary Structure 
TPAQQLQQLLKTGNIVMAPGAPDAITARLIQKAGFPAIYMTGFGATASRLGTPDIGLLS
CHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCCCCC
QTEMTTHARDMARVVDIPIIADADTGYGGPSNIHRTVREYIQAGVAAIHLEDQVAPKRCG
CHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHH
QMAGIRLMDAQENVLRLKCAIEARGRDELLIIGRTDALPAAGIDEAIRRAHLYQEAGVDL
HHHCEEEECCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCE
VFVDGIKKIAEVEAVARAVQGPKVVSIVDGNETTALTAKDLQDMGFSVVFYAVTALFTAV
EEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHH
KAVSDALAELQRAGTPKASERAMVSYAEFSALVDLDFHKDLDDRFGA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEECHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA