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The map label for this gene is cobS

Identifier: 91786642

GI number: 91786642

Start: 755904

End: 756752

Strand: Reverse

Name: cobS

Synonym: Bpro_0740

Alternate gene names: 91786642

Gene position: 756752-755904 (Counterclockwise)

Preceding gene: 91786643

Following gene: 91786641

Centisome position: 14.55

GC content: 66.08

Gene sequence:

>849_bases
ATGAATCCGATCAGCCAGTTTGTCCGCGAATACCTGCTCGCCGTCCAGTTCTTCACCCGCATACCCGTCGTGGGGCGGCT
GGCCGACTGGGTCGGCTACAGCCCCGAGTTGCTGCGCGCCAGCGCCGGTCACTTTCCAGGCGTCGGCATCCTGGTCGGCG
TCATGGCCGCGCTGGTGTATGGGCTGATACAGGCCCTGCTGCCCAACACCCCCTTCACGCCGCTGGTGGCTGCCGTGCTG
TCCACCGCCGCCACCGTGCTGTTGACGGGCGGCTTTCATGAAGACGGGCTGGCCGATGTGGCCGATGGCCTGGGCGGCAG
CCAGGACCGGGAGCGCGCGCTGGAGATCATGAAGGACTCGCGCGTGGGAGCCTTTGGCGCCATGGCGCTGATGCTCGCGC
TGCTGGGCAAGACGGCGCTGCTGGCGATGCTGGGCTCGGTCGATGTCAGCCCGGCCGAACTCGGGGACGACGCTTCTTTC
AGCAGCTGGTACATCGGCGCGGCGCTGCTGACGGGCCACGTGGTTTCGCGCGGCCTGCCGCTGCTGCTGATCTGGCTGCT
GCCGCACGTCGGCAACACCGCCAGCTCCAAATCCAAGCCGCTGGCCGACCAGATCTCCCAGGGCAGCCTGCTGGTTGCAT
TCATCTGGTCATTTGTCGTGCTGGCCCTGGCCGGCCTTGCGCTGGACGCCATCAGTTTGATCGTGGCCTGCAGCTTTTCC
CTGTTGGCGCTGTTGTGGATGGGGGCGCTCTTCAAACGGCGCTTGCAAGGTTTCACCGGCGACTGCCTGGGTGCCACGCA
GCAGGTCTGCGAAATCGCCTTCTACCTGGGTCTGGCCGTTAGTTTGTGA

Upstream 100 bases:

>100_bases
TCGGCGGCGCTGTTCCTGGACGAGATGGCCAGCTTTGACTCGGCCGGCGTGAGTGGCAAGGAGAACCCCTGAATCCGCCA
GACCCTGCGATAACCGCCCC

Downstream 100 bases:

>100_bases
ATATGGCCCCCACGCTCCCCACTTCGTGTGGTTCGCTGCCCCCCGAGGGGGCCGCCGCGCCTGCGGTCTGGCAAAGCCAG
TCCCGCGGCCCCTGCTGGAA

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MNPISQFVREYLLAVQFFTRIPVVGRLADWVGYSPELLRASAGHFPGVGILVGVMAALVYGLIQALLPNTPFTPLVAAVL
STAATVLLTGGFHEDGLADVADGLGGSQDRERALEIMKDSRVGAFGAMALMLALLGKTALLAMLGSVDVSPAELGDDASF
SSWYIGAALLTGHVVSRGLPLLLIWLLPHVGNTASSKSKPLADQISQGSLLVAFIWSFVVLALAGLALDAISLIVACSFS
LLALLWMGALFKRRLQGFTGDCLGATQQVCEIAFYLGLAVSL

Sequences:

>Translated_282_residues
MNPISQFVREYLLAVQFFTRIPVVGRLADWVGYSPELLRASAGHFPGVGILVGVMAALVYGLIQALLPNTPFTPLVAAVL
STAATVLLTGGFHEDGLADVADGLGGSQDRERALEIMKDSRVGAFGAMALMLALLGKTALLAMLGSVDVSPAELGDDASF
SSWYIGAALLTGHVVSRGLPLLLIWLLPHVGNTASSKSKPLADQISQGSLLVAFIWSFVVLALAGLALDAISLIVACSFS
LLALLWMGALFKRRLQGFTGDCLGATQQVCEIAFYLGLAVSL
>Mature_282_residues
MNPISQFVREYLLAVQFFTRIPVVGRLADWVGYSPELLRASAGHFPGVGILVGVMAALVYGLIQALLPNTPFTPLVAAVL
STAATVLLTGGFHEDGLADVADGLGGSQDRERALEIMKDSRVGAFGAMALMLALLGKTALLAMLGSVDVSPAELGDDASF
SSWYIGAALLTGHVVSRGLPLLLIWLLPHVGNTASSKSKPLADQISQGSLLVAFIWSFVVLALAGLALDAISLIVACSFS
LLALLWMGALFKRRLQGFTGDCLGATQQVCEIAFYLGLAVSL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=273, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_POLSJ (Q12FJ6)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547594.1
- STRING:   Q12FJ6
- GeneID:   4011297
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0740
- NMPDR:   fig|296591.1.peg.2132
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   ATQQVCE
- PhylomeDB:   Q12FJ6
- ProtClustDB:   CLSK951553
- BioCyc:   PSP296591:BPRO_0740-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 29573; Mature: 29573

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13f6199c)-; HASH(0x143b0cb8)-; HASH(0x14584790)-; HASH(0x1437df04)-; HASH(0x14558144)-; HASH(0x13824488)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPISQFVREYLLAVQFFTRIPVVGRLADWVGYSPELLRASAGHFPGVGILVGVMAALVY
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
GLIQALLPNTPFTPLVAAVLSTAATVLLTGGFHEDGLADVADGLGGSQDRERALEIMKDS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHC
RVGAFGAMALMLALLGKTALLAMLGSVDVSPAELGDDASFSSWYIGAALLTGHVVSRGLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHH
LLLIWLLPHVGNTASSKSKPLADQISQGSLLVAFIWSFVVLALAGLALDAISLIVACSFS
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLALLWMGALFKRRLQGFTGDCLGATQQVCEIAFYLGLAVSL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MNPISQFVREYLLAVQFFTRIPVVGRLADWVGYSPELLRASAGHFPGVGILVGVMAALVY
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
GLIQALLPNTPFTPLVAAVLSTAATVLLTGGFHEDGLADVADGLGGSQDRERALEIMKDS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHC
RVGAFGAMALMLALLGKTALLAMLGSVDVSPAELGDDASFSSWYIGAALLTGHVVSRGLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCHH
LLLIWLLPHVGNTASSKSKPLADQISQGSLLVAFIWSFVVLALAGLALDAISLIVACSFS
HHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLALLWMGALFKRRLQGFTGDCLGATQQVCEIAFYLGLAVSL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA