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The map label for this gene is dhaK [H]

Identifier: 91786610

GI number: 91786610

Start: 717930

End: 718913

Strand: Direct

Name: dhaK [H]

Synonym: Bpro_0706

Alternate gene names: 91786610

Gene position: 717930-718913 (Clockwise)

Preceding gene: 91786609

Following gene: 91786611

Centisome position: 13.81

GC content: 65.85

Gene sequence:

>984_bases
ATGAAAAAATTCGTCAACGATGTGGAGCAACTGCTGCAGGAGTCGCTGCAAGGAATGGCCGCCGCGCACAGCGACCTGCT
GACCCTGCACCTGGATCCAACATTCGTCACGCGCAAGCAGTTGCCGCAACCCAAGGTGGCCCTGATATCCGGTGGTGGTT
CAGGCCACGAGCCGCTGCACTGCGGCTTGATAGGCACAGGCATGCTCGATGCGGCCTGCCCCGGTCAGGTGTTCACCTCG
CCGACGCCGGACCAGATGATGGCCGCGGCGGCGGCGGTGGACCGCGGCAAGGGCGTGCTGTTCATCGTCAAGAACTACGC
GGGCGACGTGATGAACTTCCAGATGGCCGTGGAAATGATGCAGCAGCCCTGCGCGACCGTGCTCGTCAACGACGATGTCG
CCGTGGAGCGTTCGACCCACGGCACCGGGCGGCGCGGCGTGGCCGGCACGTTGATCGTGGAAAAGCTGGTGGGCGCGGCA
GCCGAGGCCGGTGCCAGCCTGGATCAATGCAAGATGCTGGGAGACCGGGTCAACAGCCGTACCGCGTCGATGGGCGTGGC
ATTCACCAGCTGTACCGTGCCGGCGGCAGGGCGGGCGACCTTCGACATCGCCGAGGACGAGATGGAATTCGGCGTGGGCA
TCCATGGTGAGCCCGGGCGCCGGCGCGGGCCGGTGCGCCCTGCGCAGGAACTGGTCAACGAGCTGATGGCTGCCATCCTG
GGCGACCTGCAGCCCCAGCCCGGCACAGAGGTCCTGCTGCACGTCAACGGCTTCGGGGGCACGCCCCTGATCGAGCTGTA
CCTGCTGTATCACTGCGCGAATCAACTGCTTGAGGGCGAGGGCCTGAAGGTGGCAAGGTCGCTGGTCGGCAACTACACGA
CCGCGCTGGAGATGGCCGGCGCCTCGCTGACCGTCACGCTGTTGGACGAGGAAATGAAATCCCTGTGGGATGCGCCTGTG
CACACGGCTGCGCTTCGCTGGTAG

Upstream 100 bases:

>100_bases
TCCTCGGTGAGCGCGCCCTGGGACATATCGATCCTGGTGCGAAGACCAGTCAGGTCGCGCTGTTCGCTGTGTGTGACATG
CTGACCATCGGAGCAGCATC

Downstream 100 bases:

>100_bases
GCCGGCCGCCGGTCCAGGGCTGCTGCATAAAAAAAAGACGCATCATGATGAACCGAAGAAAGGCCATCACGTTGGCCTGC
GCGGCGGCGGCCGGGCTGTC

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKKFVNDVEQLLQESLQGMAAAHSDLLTLHLDPTFVTRKQLPQPKVALISGGGSGHEPLHCGLIGTGMLDAACPGQVFTS
PTPDQMMAAAAAVDRGKGVLFIVKNYAGDVMNFQMAVEMMQQPCATVLVNDDVAVERSTHGTGRRGVAGTLIVEKLVGAA
AEAGASLDQCKMLGDRVNSRTASMGVAFTSCTVPAAGRATFDIAEDEMEFGVGIHGEPGRRRGPVRPAQELVNELMAAIL
GDLQPQPGTEVLLHVNGFGGTPLIELYLLYHCANQLLEGEGLKVARSLVGNYTTALEMAGASLTVTLLDEEMKSLWDAPV
HTAALRW

Sequences:

>Translated_327_residues
MKKFVNDVEQLLQESLQGMAAAHSDLLTLHLDPTFVTRKQLPQPKVALISGGGSGHEPLHCGLIGTGMLDAACPGQVFTS
PTPDQMMAAAAAVDRGKGVLFIVKNYAGDVMNFQMAVEMMQQPCATVLVNDDVAVERSTHGTGRRGVAGTLIVEKLVGAA
AEAGASLDQCKMLGDRVNSRTASMGVAFTSCTVPAAGRATFDIAEDEMEFGVGIHGEPGRRRGPVRPAQELVNELMAAIL
GDLQPQPGTEVLLHVNGFGGTPLIELYLLYHCANQLLEGEGLKVARSLVGNYTTALEMAGASLTVTLLDEEMKSLWDAPV
HTAALRW
>Mature_327_residues
MKKFVNDVEQLLQESLQGMAAAHSDLLTLHLDPTFVTRKQLPQPKVALISGGGSGHEPLHCGLIGTGMLDAACPGQVFTS
PTPDQMMAAAAAVDRGKGVLFIVKNYAGDVMNFQMAVEMMQQPCATVLVNDDVAVERSTHGTGRRGVAGTLIVEKLVGAA
AEAGASLDQCKMLGDRVNSRTASMGVAFTSCTVPAAGRATFDIAEDEMEFGVGIHGEPGRRRGPVRPAQELVNELMAAIL
GDLQPQPGTEVLLHVNGFGGTPLIELYLLYHCANQLLEGEGLKVARSLVGNYTTALEMAGASLTVTLLDEEMKSLWDAPV
HTAALRW

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=338, Percent_Identity=39.9408284023669, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=49.0140845070423, Blast_Score=337, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI17565018, Length=331, Percent_Identity=33.8368580060423, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321055, Length=320, Percent_Identity=33.4375, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323570, Length=341, Percent_Identity=33.7243401759531, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34617; Mature: 34617

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFVNDVEQLLQESLQGMAAAHSDLLTLHLDPTFVTRKQLPQPKVALISGGGSGHEPLH
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCCCCEE
CGLIGTGMLDAACPGQVFTSPTPDQMMAAAAAVDRGKGVLFIVKNYAGDVMNFQMAVEMM
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
QQPCATVLVNDDVAVERSTHGTGRRGVAGTLIVEKLVGAAAEAGASLDQCKMLGDRVNSR
HCCCCEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCH
TASMGVAFTSCTVPAAGRATFDIAEDEMEFGVGIHGEPGRRRGPVRPAQELVNELMAAIL
HHHCCEEEEEECCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GDLQPQPGTEVLLHVNGFGGTPLIELYLLYHCANQLLEGEGLKVARSLVGNYTTALEMAG
HCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCC
ASLTVTLLDEEMKSLWDAPVHTAALRW
CEEEEEEHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MKKFVNDVEQLLQESLQGMAAAHSDLLTLHLDPTFVTRKQLPQPKVALISGGGSGHEPLH
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCCCCEE
CGLIGTGMLDAACPGQVFTSPTPDQMMAAAAAVDRGKGVLFIVKNYAGDVMNFQMAVEMM
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
QQPCATVLVNDDVAVERSTHGTGRRGVAGTLIVEKLVGAAAEAGASLDQCKMLGDRVNSR
HCCCCEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCH
TASMGVAFTSCTVPAAGRATFDIAEDEMEFGVGIHGEPGRRRGPVRPAQELVNELMAAIL
HHHCCEEEEEECCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GDLQPQPGTEVLLHVNGFGGTPLIELYLLYHCANQLLEGEGLKVARSLVGNYTTALEMAG
HCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCC
ASLTVTLLDEEMKSLWDAPVHTAALRW
CEEEEEEHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]