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The map label for this gene is ooxB [H]

Identifier: 91786592

GI number: 91786592

Start: 701563

End: 702711

Strand: Reverse

Name: ooxB [H]

Synonym: Bpro_0688

Alternate gene names: 91786592

Gene position: 702711-701563 (Counterclockwise)

Preceding gene: 91786593

Following gene: 91786591

Centisome position: 13.51

GC content: 69.54

Gene sequence:

>1149_bases
ATGAGCAACGCCACGCACAACGCAACCGCCTACGACGCGATCGTCATCGGCGGCGGCGTCATGGGTTGCTCGACCGCGTT
GCATCTGGCGCAGGGCGGCATGCGGGTGGCCCTGGTCGATCGCGGGCCGCTGTGTCGCGAAGCCTCGGGCGTCAACGCCG
GCACGTTGACACTGCACATGACGCGCGCCGCGCTCGTGCCCTACGCGATGCGCGCCTGGCAGATGTGGATGAACGCCGAA
AAGTGGCTCGGCATGGGCGTGCTCGCGACCCATGTGCCGGGGCTCACGCTGGCCTTCACCGAGGCCGAGTGCGAACTCGT
CGAGCTGCGCGCCAAAGCCCGCCGCGAACAAGGCGCGCCGATCGAGGTCATCACGCCCGAGCGGGCGCGCGTGATCGAGC
CCGGCCTGCATCCTGGCCTGCTGAAGGCGGGTTACTGCGGGATGGACGGTTTCGCGAGCGCCTACCTCACGGGCCGGGCG
TTCGGGCATGCGCTCAAGCTTGCCGGCGTCGAGGTGCTGGAGAACGCGCCCGTCGAAGGCATAGCTTCAGGCGACGCGGG
CCACGTCATCCGTCTTGCCGCCGCCACCGGCCGCGCGCCGTTGCACGCCAGGCGGGTGGTGCTGGCCGGCGGCGTGTGGC
TCGAGAACATGCTGGCCTGGCTCGGCGTGCAGATGCCGATCAAGGTGCTGATCAACCAGCTCATCATCACCGAGCGCATC
CGTCCCGTGATGCGGACCGTGCTCAGCGTCGCCAACGGCCTGCTGTCACTCAAGCAATTTGCCAATGGCACCGTACTGAT
CGGTGGCGGCTGGCAGGGCGAGGGCGACCGCGAGCGCGGCGGTGTCGAGGTCCGACCGCAGAACCTCGTGGGCAACATGC
GCCTGGCCGCATACGCGGTGCCGGCGCTGGCCGAGGCCCGCATTGCGCGCATCTGGCTGGGCCTTGAGGCCGAGACCGCC
GATGCGATGCCGATCATCGGCGACGTGCCCGGTGTGCCACAGGCCTATGTGGTGGGCAGCGCGCACTCGGGCTACACCAG
CGGCCCGTTCATGGGCCGGATCATGGCCCAGCACATCCTCGGCCAGCAGCCCGACCTGCCTCTTTTCGATCCCGCCCGCC
TGGTGGGCATGCCGATGCCCGGCGCTTGA

Upstream 100 bases:

>100_bases
GTCGCTTCACGGGCCGCGCGCCGCTGCGCCCCATTTCGCTCGCCGAGGTGGCGGGCGACTACACCTACGCCGACATCCCG
ATTCCGAAGGCGGCTCCGCT

Downstream 100 bases:

>100_bases
AATCTCCCATTCCATTCCATTCCATGATTCCCAAACGACCTGACGCTTTTCAAGGCATCTATGCCGCCACCCTGTGCCCG
CTGCATGGCGATGGCCGCAT

Product: FAD dependent oxidoreductase

Products: glycine; formaldehyde; H2O2

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MSNATHNATAYDAIVIGGGVMGCSTALHLAQGGMRVALVDRGPLCREASGVNAGTLTLHMTRAALVPYAMRAWQMWMNAE
KWLGMGVLATHVPGLTLAFTEAECELVELRAKARREQGAPIEVITPERARVIEPGLHPGLLKAGYCGMDGFASAYLTGRA
FGHALKLAGVEVLENAPVEGIASGDAGHVIRLAAATGRAPLHARRVVLAGGVWLENMLAWLGVQMPIKVLINQLIITERI
RPVMRTVLSVANGLLSLKQFANGTVLIGGGWQGEGDRERGGVEVRPQNLVGNMRLAAYAVPALAEARIARIWLGLEAETA
DAMPIIGDVPGVPQAYVVGSAHSGYTSGPFMGRIMAQHILGQQPDLPLFDPARLVGMPMPGA

Sequences:

>Translated_382_residues
MSNATHNATAYDAIVIGGGVMGCSTALHLAQGGMRVALVDRGPLCREASGVNAGTLTLHMTRAALVPYAMRAWQMWMNAE
KWLGMGVLATHVPGLTLAFTEAECELVELRAKARREQGAPIEVITPERARVIEPGLHPGLLKAGYCGMDGFASAYLTGRA
FGHALKLAGVEVLENAPVEGIASGDAGHVIRLAAATGRAPLHARRVVLAGGVWLENMLAWLGVQMPIKVLINQLIITERI
RPVMRTVLSVANGLLSLKQFANGTVLIGGGWQGEGDRERGGVEVRPQNLVGNMRLAAYAVPALAEARIARIWLGLEAETA
DAMPIIGDVPGVPQAYVVGSAHSGYTSGPFMGRIMAQHILGQQPDLPLFDPARLVGMPMPGA
>Mature_381_residues
SNATHNATAYDAIVIGGGVMGCSTALHLAQGGMRVALVDRGPLCREASGVNAGTLTLHMTRAALVPYAMRAWQMWMNAEK
WLGMGVLATHVPGLTLAFTEAECELVELRAKARREQGAPIEVITPERARVIEPGLHPGLLKAGYCGMDGFASAYLTGRAF
GHALKLAGVEVLENAPVEGIASGDAGHVIRLAAATGRAPLHARRVVLAGGVWLENMLAWLGVQMPIKVLINQLIITERIR
PVMRTVLSVANGLLSLKQFANGTVLIGGGWQGEGDRERGGVEVRPQNLVGNMRLAAYAVPALAEARIARIWLGLEAETAD
AMPIIGDVPGVPQAYVVGSAHSGYTSGPFMGRIMAQHILGQQPDLPLFDPARLVGMPMPGA

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI24797151, Length=396, Percent_Identity=23.7373737373737, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI197927446, Length=386, Percent_Identity=25.6476683937824, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI21361378, Length=386, Percent_Identity=25.6476683937824, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130091, Length=373, Percent_Identity=24.1286863270777, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.5.3.1

Molecular weight: Translated: 40340; Mature: 40209

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNATHNATAYDAIVIGGGVMGCSTALHLAQGGMRVALVDRGPLCREASGVNAGTLTLHM
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
TRAALVPYAMRAWQMWMNAEKWLGMGVLATHVPGLTLAFTEAECELVELRAKARREQGAP
HHHHHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
IEVITPERARVIEPGLHPGLLKAGYCGMDGFASAYLTGRAFGHALKLAGVEVLENAPVEG
EEEECCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IASGDAGHVIRLAAATGRAPLHARRVVLAGGVWLENMLAWLGVQMPIKVLINQLIITERI
CCCCCCCCEEEEEECCCCCCHHHCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RPVMRTVLSVANGLLSLKQFANGTVLIGGGWQGEGDRERGGVEVRPQNLVGNMRLAAYAV
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECHHHHHCCCHHHHHHH
PALAEARIARIWLGLEAETADAMPIIGDVPGVPQAYVVGSAHSGYTSGPFMGRIMAQHIL
HHHHHHHEEHEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHC
GQQPDLPLFDPARLVGMPMPGA
CCCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
SNATHNATAYDAIVIGGGVMGCSTALHLAQGGMRVALVDRGPLCREASGVNAGTLTLHM
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEE
TRAALVPYAMRAWQMWMNAEKWLGMGVLATHVPGLTLAFTEAECELVELRAKARREQGAP
HHHHHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
IEVITPERARVIEPGLHPGLLKAGYCGMDGFASAYLTGRAFGHALKLAGVEVLENAPVEG
EEEECCCCCEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IASGDAGHVIRLAAATGRAPLHARRVVLAGGVWLENMLAWLGVQMPIKVLINQLIITERI
CCCCCCCCEEEEEECCCCCCHHHCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RPVMRTVLSVANGLLSLKQFANGTVLIGGGWQGEGDRERGGVEVRPQNLVGNMRLAAYAV
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECHHHHHCCCHHHHHHH
PALAEARIARIWLGLEAETADAMPIIGDVPGVPQAYVVGSAHSGYTSGPFMGRIMAQHIL
HHHHHHHEEHEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHC
GQQPDLPLFDPARLVGMPMPGA
CCCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sarcosine; H2O; O2

Specific reaction: sarcosine + H2O + O2 = glycine + formaldehyde + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]