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The map label for this gene is hyuA [H]

Identifier: 91786590

GI number: 91786590

Start: 699170

End: 700579

Strand: Reverse

Name: hyuA [H]

Synonym: Bpro_0686

Alternate gene names: 91786590

Gene position: 700579-699170 (Counterclockwise)

Preceding gene: 91786591

Following gene: 91786589

Centisome position: 13.47

GC content: 65.32

Gene sequence:

>1410_bases
ATGTCCCGCAAGATCATACGCAACGGCCGCATCGTCACGGCCGTGGACGACTACATCGCCGATCTGCTGATCGACGACGG
CCGGATCGTCGCTATCGGCCGCTCCCTCAATGCGGACCCCGGGGTGGAAGTCATCGACGCCACCGGTCTGCTCGTGCTGC
CTGGCGGCGTGGACTGCCACACGCACATGGACAACACTTTCGGCGACTCCACCACCTGCGACAGTTTCGAGTCCGGCACG
CGTTCGGCGGCCTTCGGCGGCACAACGACGATCGTGGACTTCGCGTTCCAGCGCAAAGACGTGAGCGTGCTCGAAGCGAT
CGAGCGCGCCCAGGCCAAGGGCGTGGAAGCCTGCATCGACTATGGCTTTCACGTCATCGTGACCGGCGTCAACGACCAGA
CCCTGGCCGACATGCAGCACGCGATCCGGCATGAAGGCGTGTCGAGCTTCAAGATGTTCATGGCGTATCCGGGCTCGGTG
ATGGTCGACGACGCGGCCATCTTCCAGGCCATGCGCATGGTGGGCCAGCACGGCGGGATGATCGCTCTGCATGCCGAGAA
CGGCACCATCATCGAGCTTCTCATCAAGGAAGCGCTGGCGCAGGGCCACACGGCGCCAAAGTACCATGCGCTCACGCGGC
CCGCCATCATGGAGGGCGAGGCCACGCACCGCGGCATCAAGCTCGCCGAACTCGCCGGCGCGCCGGTGTACTTCGTGCAT
CTTTCGGCCAGGGAAGCGCTCAAGCACGTGATCGAAGCGCGCGACATGGGCGTGCCGGTGTTCGCCGAAACCTGCCCGCA
TTACCTCTTCTTTGACGACAGTGCCTACGACAACGAAGACTTCGATCTGGCGCGCTACGTGATGAGCCCACCGTTGCGCT
CCAAGGAATCGCAGCAGGCGCTCTGGACCGCACTGCGCACCGACGACCTGCAGCTCGTGTCGACCGACCATTGCCCGTTC
TGCATGAAGGAAGGCCATCTTGGCCGCGTCAACCAGAAGCCGTACGGGCGCGACGATTTCTCGAAGATCCCGAATGGCGT
GCCGGGCGTCGAAACACGCATGGCGACCCTCCACGACGGCGGCGTGCGGTCCGGTCGCATCTCGCTCAACCGCTTCGTCG
AGTTGACCTCGACGGCGCCGTCGAAGCTCTTCGGCCTGTTTCCGCGCAAAGGCACCGTCGCGGTCGGCAGCGACGCCGAC
CTCGTGCTGTTCGATCCCGGCGCCCAGCACGTCCTTTCGGCGAGCACCCAGCACGGCAACTGCGATTTCACACTCTTCGA
AGGCAAGGGCATCACCGGCCAGGTGAGCAAGGTGCTGCTGCGCGGCGAACTCATCGTCGACGGCGACCGGTGGCTGGGCA
GGGCCGGCAGCGGGCGCTTCGTGCCGCGCGGCGAATCGGGCGGCTGGTGA

Upstream 100 bases:

>100_bases
GCCGATCGGGCTGCTTCCGGCCGAAGAGGTCGCCGCACTGGAGCGTGCGCTGGCCGAAGCCGGCGTCGGCGCGTAATTCG
AATCTTTAGGAGCTACTGCC

Downstream 100 bases:

>100_bases
GCGTCCGTGCCAACCTACGACTACGCTTGTACGCGCTGCGGCCCTTTCACCGCGCGGCGGCCGCTGGCCGTATTCGACCA
ACCGGTGCCTTGCCCCGCAT

Product: dihydropyrimidinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSRKIIRNGRIVTAVDDYIADLLIDDGRIVAIGRSLNADPGVEVIDATGLLVLPGGVDCHTHMDNTFGDSTTCDSFESGT
RSAAFGGTTTIVDFAFQRKDVSVLEAIERAQAKGVEACIDYGFHVIVTGVNDQTLADMQHAIRHEGVSSFKMFMAYPGSV
MVDDAAIFQAMRMVGQHGGMIALHAENGTIIELLIKEALAQGHTAPKYHALTRPAIMEGEATHRGIKLAELAGAPVYFVH
LSAREALKHVIEARDMGVPVFAETCPHYLFFDDSAYDNEDFDLARYVMSPPLRSKESQQALWTALRTDDLQLVSTDHCPF
CMKEGHLGRVNQKPYGRDDFSKIPNGVPGVETRMATLHDGGVRSGRISLNRFVELTSTAPSKLFGLFPRKGTVAVGSDAD
LVLFDPGAQHVLSASTQHGNCDFTLFEGKGITGQVSKVLLRGELIVDGDRWLGRAGSGRFVPRGESGGW

Sequences:

>Translated_469_residues
MSRKIIRNGRIVTAVDDYIADLLIDDGRIVAIGRSLNADPGVEVIDATGLLVLPGGVDCHTHMDNTFGDSTTCDSFESGT
RSAAFGGTTTIVDFAFQRKDVSVLEAIERAQAKGVEACIDYGFHVIVTGVNDQTLADMQHAIRHEGVSSFKMFMAYPGSV
MVDDAAIFQAMRMVGQHGGMIALHAENGTIIELLIKEALAQGHTAPKYHALTRPAIMEGEATHRGIKLAELAGAPVYFVH
LSAREALKHVIEARDMGVPVFAETCPHYLFFDDSAYDNEDFDLARYVMSPPLRSKESQQALWTALRTDDLQLVSTDHCPF
CMKEGHLGRVNQKPYGRDDFSKIPNGVPGVETRMATLHDGGVRSGRISLNRFVELTSTAPSKLFGLFPRKGTVAVGSDAD
LVLFDPGAQHVLSASTQHGNCDFTLFEGKGITGQVSKVLLRGELIVDGDRWLGRAGSGRFVPRGESGGW
>Mature_468_residues
SRKIIRNGRIVTAVDDYIADLLIDDGRIVAIGRSLNADPGVEVIDATGLLVLPGGVDCHTHMDNTFGDSTTCDSFESGTR
SAAFGGTTTIVDFAFQRKDVSVLEAIERAQAKGVEACIDYGFHVIVTGVNDQTLADMQHAIRHEGVSSFKMFMAYPGSVM
VDDAAIFQAMRMVGQHGGMIALHAENGTIIELLIKEALAQGHTAPKYHALTRPAIMEGEATHRGIKLAELAGAPVYFVHL
SAREALKHVIEARDMGVPVFAETCPHYLFFDDSAYDNEDFDLARYVMSPPLRSKESQQALWTALRTDDLQLVSTDHCPFC
MKEGHLGRVNQKPYGRDDFSKIPNGVPGVETRMATLHDGGVRSGRISLNRFVELTSTAPSKLFGLFPRKGTVAVGSDADL
VLFDPGAQHVLSASTQHGNCDFTLFEGKGITGQVSKVLLRGELIVDGDRWLGRAGSGRFVPRGESGGW

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503375, Length=471, Percent_Identity=41.6135881104034, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI19923821, Length=463, Percent_Identity=36.9330453563715, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI4503379, Length=464, Percent_Identity=35.7758620689655, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI190194363, Length=464, Percent_Identity=36.2068965517241, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI4503051, Length=464, Percent_Identity=34.4827586206897, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI62422571, Length=464, Percent_Identity=34.4827586206897, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI4503377, Length=464, Percent_Identity=32.9741379310345, Blast_Score=271, Evalue=7e-73,
Organism=Homo sapiens, GI18105007, Length=479, Percent_Identity=23.5908141962422, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87082175, Length=464, Percent_Identity=40.5172413793103, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1786722, Length=469, Percent_Identity=30.7036247334755, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17539558, Length=462, Percent_Identity=39.3939393939394, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI71989490, Length=464, Percent_Identity=38.5775862068966, Blast_Score=308, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI86575075, Length=446, Percent_Identity=30.4932735426009, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI193204318, Length=436, Percent_Identity=24.0825688073394, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322218, Length=403, Percent_Identity=29.2803970223325, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI221377917, Length=468, Percent_Identity=38.2478632478632, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI17137462, Length=465, Percent_Identity=38.0645161290323, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24644287, Length=302, Percent_Identity=36.4238410596026, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24644289, Length=231, Percent_Identity=38.0952380952381, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24642586, Length=403, Percent_Identity=25.8064516129032, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI18859883, Length=422, Percent_Identity=25.8293838862559, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 50835; Mature: 50704

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKIIRNGRIVTAVDDYIADLLIDDGRIVAIGRSLNADPGVEVIDATGLLVLPGGVDCH
CCCCEECCCCEEEEHHHHHHHHEECCCEEEEECCCCCCCCCCEEEECCCEEEECCCCEEE
THMDNTFGDSTTCDSFESGTRSAAFGGTTTIVDFAFQRKDVSVLEAIERAQAKGVEACID
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHCCHHHHHH
YGFHVIVTGVNDQTLADMQHAIRHEGVSSFKMFMAYPGSVMVDDAAIFQAMRMVGQHGGM
CCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCE
IALHAENGTIIELLIKEALAQGHTAPKYHALTRPAIMEGEATHRGIKLAELAGAPVYFVH
EEEECCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEECCCCHHCCEEEEHHCCCCEEEEE
LSAREALKHVIEARDMGVPVFAETCPHYLFFDDSAYDNEDFDLARYVMSPPLRSKESQQA
ECHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LWTALRTDDLQLVSTDHCPFCMKEGHLGRVNQKPYGRDDFSKIPNGVPGVETRMATLHDG
HHHHHCCCCEEEEECCCCCHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHEEEEEECC
GVRSGRISLNRFVELTSTAPSKLFGLFPRKGTVAVGSDADLVLFDPGAQHVLSASTQHGN
CCCCCEEEHHHEEEECCCCCHHHHCCCCCCCEEEECCCCCEEEECCCHHHHHHCCCCCCC
CDFTLFEGKGITGQVSKVLLRGELIVDGDRWLGRAGSGRFVPRGESGGW
CCEEEEECCCCCHHHHHHHEECEEEEECCHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRKIIRNGRIVTAVDDYIADLLIDDGRIVAIGRSLNADPGVEVIDATGLLVLPGGVDCH
CCCEECCCCEEEEHHHHHHHHEECCCEEEEECCCCCCCCCCEEEECCCEEEECCCCEEE
THMDNTFGDSTTCDSFESGTRSAAFGGTTTIVDFAFQRKDVSVLEAIERAQAKGVEACID
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHCCHHHHHH
YGFHVIVTGVNDQTLADMQHAIRHEGVSSFKMFMAYPGSVMVDDAAIFQAMRMVGQHGGM
CCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCE
IALHAENGTIIELLIKEALAQGHTAPKYHALTRPAIMEGEATHRGIKLAELAGAPVYFVH
EEEECCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEECCCCHHCCEEEEHHCCCCEEEEE
LSAREALKHVIEARDMGVPVFAETCPHYLFFDDSAYDNEDFDLARYVMSPPLRSKESQQA
ECHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LWTALRTDDLQLVSTDHCPFCMKEGHLGRVNQKPYGRDDFSKIPNGVPGVETRMATLHDG
HHHHHCCCCEEEEECCCCCHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHEEEEEECC
GVRSGRISLNRFVELTSTAPSKLFGLFPRKGTVAVGSDADLVLFDPGAQHVLSASTQHGN
CCCCCEEEHHHEEEECCCCCHHHHCCCCCCCEEEECCCCCEEEECCCHHHHHHCCCCCCC
CDFTLFEGKGITGQVSKVLLRGELIVDGDRWLGRAGSGRFVPRGESGGW
CCEEEEECCCCCHHHHHHHEECEEEEECCHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]