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The map label for this gene is rluF [H]

Identifier: 91786512

GI number: 91786512

Start: 613910

End: 614710

Strand: Direct

Name: rluF [H]

Synonym: Bpro_0606

Alternate gene names: 91786512

Gene position: 613910-614710 (Clockwise)

Preceding gene: 91786511

Following gene: 91786513

Centisome position: 11.81

GC content: 66.04

Gene sequence:

>801_bases
GTGCGTATCCCGAAGGCCGCCGCCCCGGCCGCCACCGCTTCCGCATCGTCCACCGTGCGGCTGAACAAACGCATGGCCGA
GTTGGGCCTGTGCTCACGCCGCGAAGCCGACGACTGGATTGCCCGTGGCTGGGTGCTGGTCAATGGCGCGCCCGCCGTGA
TGGGCCAGCCGGTCGCCCCTGACGCGCGCATCGAAGTGGATCGCCAGGCCGAGCAGCAGCAGCGCCAGCAGGTCACCATC
CTGATCAACAAGCCCGTTGGCTATGTCAGCGGCCAGGCTGAAGACGGCCATGAGCCTGCGGTGGTGCTGGTACAGCCGCA
AAACCGCTGGCGCGAGTGCAACAGCCGCATGCGCTGGGGTCCCGAGCAGTTGCGCGGCCTGGCGCCGGCCGGTCGCCTGG
ACATTGACTCCATCGGCTTGCTGGTGCTGACACAGGACGGGCGCGTGGCGCGCCAGCTCATCGGTGAAAACTCGGAGATA
GAAAAAGAGTACCTGGTGCGGGTCAGCTATGGCGCCGAAACGAGCAACGTGCAGGCGGTGTTCCCGCGTGAAAAGCTGGC
CTTGCTGCGCCATGGCCTGAGCCTCGACGGGCAGGCCTTGCGGCCGGCGCAGGTGGACTGGCAAAACCCCGAGCAACTGC
GGTTTGTGCTCAAGGAAGGCAAGAAGCGCCAGATTCGCCGCATGTGCGAGCAGGTGGGGCTGTTTGTGACCGGACTCAAG
CGCGTGCGAATCGGTCGGGTCAACCTCGGCCATTTGCCGGTAGGCCAGTGGCGCTACCTGGCTGCGCACGAATCTTTTTA
G

Upstream 100 bases:

>100_bases
AATGCCGCCCTTCATGCAAGAGCCAGGCAAGCTATAAAATAAATAGCAAATGCAAGATCCATCCAAACCCGGAGCGGGGC
CCAAACCAGGTGGCACGGCC

Downstream 100 bases:

>100_bases
CCTTTGCCATCAGGGTAGTGATCGGCAGCGGTCGGTAGCGCTGCGGGAACGCCAGAGTTCCCCCCTCTCATTGCGTCGGG
CAGCGCTGCAGGCTCACTCT

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRIPKAAAPAATASASSTVRLNKRMAELGLCSRREADDWIARGWVLVNGAPAVMGQPVAPDARIEVDRQAEQQQRQQVTI
LINKPVGYVSGQAEDGHEPAVVLVQPQNRWRECNSRMRWGPEQLRGLAPAGRLDIDSIGLLVLTQDGRVARQLIGENSEI
EKEYLVRVSYGAETSNVQAVFPREKLALLRHGLSLDGQALRPAQVDWQNPEQLRFVLKEGKKRQIRRMCEQVGLFVTGLK
RVRIGRVNLGHLPVGQWRYLAAHESF

Sequences:

>Translated_266_residues
MRIPKAAAPAATASASSTVRLNKRMAELGLCSRREADDWIARGWVLVNGAPAVMGQPVAPDARIEVDRQAEQQQRQQVTI
LINKPVGYVSGQAEDGHEPAVVLVQPQNRWRECNSRMRWGPEQLRGLAPAGRLDIDSIGLLVLTQDGRVARQLIGENSEI
EKEYLVRVSYGAETSNVQAVFPREKLALLRHGLSLDGQALRPAQVDWQNPEQLRFVLKEGKKRQIRRMCEQVGLFVTGLK
RVRIGRVNLGHLPVGQWRYLAAHESF
>Mature_266_residues
MRIPKAAAPAATASASSTVRLNKRMAELGLCSRREADDWIARGWVLVNGAPAVMGQPVAPDARIEVDRQAEQQQRQQVTI
LINKPVGYVSGQAEDGHEPAVVLVQPQNRWRECNSRMRWGPEQLRGLAPAGRLDIDSIGLLVLTQDGRVARQLIGENSEI
EKEYLVRVSYGAETSNVQAVFPREKLALLRHGLSLDGQALRPAQVDWQNPEQLRFVLKEGKKRQIRRMCEQVGLFVTGLK
RVRIGRVNLGHLPVGQWRYLAAHESF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=252, Percent_Identity=35.7142857142857, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788510, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=30.5882352941176, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081838, Length=154, Percent_Identity=33.1168831168831, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29731; Mature: 29731

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPKAAAPAATASASSTVRLNKRMAELGLCSRREADDWIARGWVLVNGAPAVMGQPVAP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCCHHCCCCCCC
DARIEVDRQAEQQQRQQVTILINKPVGYVSGQAEDGHEPAVVLVQPQNRWRECNSRMRWG
CCEEEEHHHHHHHHHCEEEEEEECCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
PEQLRGLAPAGRLDIDSIGLLVLTQDGRVARQLIGENSEIEKEYLVRVSYGAETSNVQAV
HHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHEEEEECCCCCCCEEEE
FPREKLALLRHGLSLDGQALRPAQVDWQNPEQLRFVLKEGKKRQIRRMCEQVGLFVTGLK
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RVRIGRVNLGHLPVGQWRYLAAHESF
HEEECCEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MRIPKAAAPAATASASSTVRLNKRMAELGLCSRREADDWIARGWVLVNGAPAVMGQPVAP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCEEEECCCCHHCCCCCCC
DARIEVDRQAEQQQRQQVTILINKPVGYVSGQAEDGHEPAVVLVQPQNRWRECNSRMRWG
CCEEEEHHHHHHHHHCEEEEEEECCCCEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
PEQLRGLAPAGRLDIDSIGLLVLTQDGRVARQLIGENSEIEKEYLVRVSYGAETSNVQAV
HHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCHHHEEEEECCCCCCCEEEE
FPREKLALLRHGLSLDGQALRPAQVDWQNPEQLRFVLKEGKKRQIRRMCEQVGLFVTGLK
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RVRIGRVNLGHLPVGQWRYLAAHESF
HEEECCEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]