BacMap

Definition	Polaromonas sp. JS666 chromosome, complete genome.
Accession	NC_007948
Length	5,200,264

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The map label for this gene is braF [H]

Identifier: 91786509

GI number: 91786509

Start: 609831

End: 610613

Strand: Reverse

Name: braF [H]

Synonym: Bpro_0603

Alternate gene names: 91786509

Gene position: 610613-609831 (Counterclockwise)

Preceding gene: 91786510

Following gene: 91786508

Centisome position: 11.74

GC content: 59.77

Gene sequence:

>783_bases
ATGCACAAGAAAATCGGCGACGTCATTCTCGACGTCAGCAACATCTCCCTGAGCTTTGGCGGCGTCAAGGCGCTGTCCGA
TATTTCCTTCAATGTGAAGGAGCACGAGATACGCGCCATCATCGGCCCCAACGGCGCAGGCAAGAGCTCCATGCTCAACT
GCATCAACGGCGTCTATTCGCCCCAGCAGGGAGCCATCACCTTTCGCGGCCAGACCTTCAAGCACATGAACAGCCACCAG
GTCGCGGTGATGGGTGTGGCCCGCACCTTCCAGAACCTGGCGCTGTTCAAGGGCATGAGCGTGCTGGACAACATCATGTC
GGGCCGCAACCTCAAGATCAAAAGCAACCTGCTGCTGCAGGCGCTGCGGATTGGCCCGGCCGAGAAAGAGGAAATCATGC
ACCGCGAGGCGGTCGAGAAAATCATCGACTTTCTCGAGATCCAGGCCTTCCGCAAAACACCGGTCGGCCAGTTGCCCTAC
GGACTGCAAAAACGCGTCGACCTGGGCCGGGCGCTGGCCATGGAGCCGCAGGTCCTGCTGCTTGACGAGCCCATGGCCGG
CATGAACGTCGAAGAAAAGCAGGACATGTGCCGCTTTGTCCTCGACGTGAACGACGAGTTCGGCACCACCGTGGTATTGA
TCGAGCACGACATGGGCGTGGTGATGGACATTTCCGACCGCGTGGTGGTGCTGGACTACGGCAAGAAGATTGGCGACGGC
ACCCCCGATGAAGTCCGCAACAACCCCGACGTGATCCGGGCTTACCTGGGTGTGGAGCACTGA

Upstream 100 bases:

>100_bases
CAGCTCGAGCCGCGGAACCGGCTTTGCCGGGCCGCAGGCGGGCGGCCCCCTCGGGGGGCAGCGCAGTACACGGAGTGACA
AGCGTGGGGGTACCTATCTG

Downstream 100 bases:

>100_bases
TGGAAATGGCACACCCCTGTTGCGGGCTCGCTGCGCGTAGCCGCCTCTCCCCTTGCAGGGGACGCCGCTTGCAGCCCGGC
AAAGCCGGTTCTGCGGCGGC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MHKKIGDVILDVSNISLSFGGVKALSDISFNVKEHEIRAIIGPNGAGKSSMLNCINGVYSPQQGAITFRGQTFKHMNSHQ
VAVMGVARTFQNLALFKGMSVLDNIMSGRNLKIKSNLLLQALRIGPAEKEEIMHREAVEKIIDFLEIQAFRKTPVGQLPY
GLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMCRFVLDVNDEFGTTVVLIEHDMGVVMDISDRVVVLDYGKKIGDG
TPDEVRNNPDVIRAYLGVEH

Sequences:

>Translated_260_residues
MHKKIGDVILDVSNISLSFGGVKALSDISFNVKEHEIRAIIGPNGAGKSSMLNCINGVYSPQQGAITFRGQTFKHMNSHQ
VAVMGVARTFQNLALFKGMSVLDNIMSGRNLKIKSNLLLQALRIGPAEKEEIMHREAVEKIIDFLEIQAFRKTPVGQLPY
GLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMCRFVLDVNDEFGTTVVLIEHDMGVVMDISDRVVVLDYGKKIGDG
TPDEVRNNPDVIRAYLGVEH
>Mature_260_residues
MHKKIGDVILDVSNISLSFGGVKALSDISFNVKEHEIRAIIGPNGAGKSSMLNCINGVYSPQQGAITFRGQTFKHMNSHQ
VAVMGVARTFQNLALFKGMSVLDNIMSGRNLKIKSNLLLQALRIGPAEKEEIMHREAVEKIIDFLEIQAFRKTPVGQLPY
GLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMCRFVLDVNDEFGTTVVLIEHDMGVVMDISDRVVVLDYGKKIGDG
TPDEVRNNPDVIRAYLGVEH

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI47078218, Length=219, Percent_Identity=26.4840182648402, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI27477115, Length=190, Percent_Identity=29.4736842105263, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45446740, Length=219, Percent_Identity=26.4840182648402, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI9961252, Length=230, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4505771, Length=230, Percent_Identity=28.2608695652174, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI153792144, Length=226, Percent_Identity=26.5486725663717, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI31657092, Length=230, Percent_Identity=23.4782608695652, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI42741659, Length=230, Percent_Identity=27.3913043478261, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI6005701, Length=214, Percent_Identity=27.5700934579439, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI27436953, Length=172, Percent_Identity=30.8139534883721, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI27881501, Length=214, Percent_Identity=24.7663551401869, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI30795238, Length=214, Percent_Identity=24.7663551401869, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI21536378, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI148612844, Length=232, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI255708477, Length=232, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI9961250, Length=237, Percent_Identity=27.4261603375527, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI21536376, Length=231, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI105990541, Length=227, Percent_Identity=22.4669603524229, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=36.9047619047619, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1788761, Length=240, Percent_Identity=31.25, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI87081791, Length=241, Percent_Identity=30.7053941908714, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI48995001, Length=233, Percent_Identity=30.0429184549356, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787029, Length=255, Percent_Identity=29.0196078431373, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1787712, Length=227, Percent_Identity=30.8370044052863, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788472, Length=233, Percent_Identity=29.1845493562232, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790467, Length=240, Percent_Identity=30, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789593, Length=248, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789032, Length=256, Percent_Identity=30.078125, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI87081709, Length=241, Percent_Identity=25.7261410788382, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788450, Length=253, Percent_Identity=27.2727272727273, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789672, Length=262, Percent_Identity=27.0992366412214, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787370, Length=241, Percent_Identity=26.9709543568465, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786872, Length=250, Percent_Identity=29.2, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788225, Length=253, Percent_Identity=30.0395256916996, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786563, Length=226, Percent_Identity=29.646017699115, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790525, Length=250, Percent_Identity=26, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788210, Length=248, Percent_Identity=27.4193548387097, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790739, Length=255, Percent_Identity=28.2352941176471, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788644, Length=260, Percent_Identity=29.2307692307692, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786398, Length=247, Percent_Identity=27.1255060728745, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790190, Length=235, Percent_Identity=29.3617021276596, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789991, Length=234, Percent_Identity=28.2051282051282, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87082267, Length=241, Percent_Identity=27.8008298755187, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=27.1186440677966, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994997, Length=245, Percent_Identity=26.1224489795918, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788897, Length=213, Percent_Identity=31.4553990610329, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87081782, Length=244, Percent_Identity=28.6885245901639, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789586, Length=251, Percent_Identity=26.6932270916335, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786253, Length=230, Percent_Identity=30.8695652173913, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1789891, Length=213, Percent_Identity=28.6384976525822, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786803, Length=242, Percent_Identity=25.2066115702479, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789962, Length=230, Percent_Identity=26.5217391304348, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786345, Length=240, Percent_Identity=28.3333333333333, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082268, Length=251, Percent_Identity=26.2948207171315, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787759, Length=258, Percent_Identity=27.1317829457364, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1786319, Length=210, Percent_Identity=27.6190476190476, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790162, Length=247, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789873, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI145693107, Length=261, Percent_Identity=24.5210727969349, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI48994883, Length=261, Percent_Identity=23.7547892720307, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787500, Length=232, Percent_Identity=23.2758620689655, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790544, Length=247, Percent_Identity=24.2914979757085, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994943, Length=242, Percent_Identity=22.7272727272727, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367384, Length=232, Percent_Identity=28.8793103448276, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787547, Length=256, Percent_Identity=21.875, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787792, Length=246, Percent_Identity=23.5772357723577, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786703, Length=214, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788053, Length=232, Percent_Identity=25.8620689655172, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081834, Length=232, Percent_Identity=24.5689655172414, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786698, Length=228, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787164, Length=215, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786654, Length=241, Percent_Identity=26.1410788381743, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787112, Length=223, Percent_Identity=26.457399103139, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788506, Length=268, Percent_Identity=23.8805970149254, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786981, Length=230, Percent_Identity=27.3913043478261, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787105, Length=247, Percent_Identity=23.8866396761134, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534520, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17533971, Length=231, Percent_Identity=25.974025974026, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71992290, Length=225, Percent_Identity=27.5555555555556, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17510237, Length=242, Percent_Identity=22.7272727272727, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17511077, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=243, Percent_Identity=23.0452674897119, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=243, Percent_Identity=23.0452674897119, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=222, Percent_Identity=25.6756756756757, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=243, Percent_Identity=23.0452674897119, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=229, Percent_Identity=27.0742358078603, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17569145, Length=233, Percent_Identity=26.1802575107296, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=213, Percent_Identity=25.3521126760563, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=264, Percent_Identity=23.8636363636364, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221512771, Length=239, Percent_Identity=27.1966527196653, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648314, Length=250, Percent_Identity=26, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666092, Length=226, Percent_Identity=24.7787610619469, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571209, Length=196, Percent_Identity=25.5102040816327, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24643674, Length=239, Percent_Identity=25.1046025104602, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI28574744, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581191, Length=224, Percent_Identity=26.3392857142857, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581189, Length=224, Percent_Identity=26.3392857142857, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581193, Length=224, Percent_Identity=26.3392857142857, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581195, Length=224, Percent_Identity=26.3392857142857, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573571, Length=234, Percent_Identity=25.6410256410256, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573573, Length=228, Percent_Identity=25.8771929824561, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581387, Length=244, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 28774; Mature: 28774

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKKIGDVILDVSNISLSFGGVKALSDISFNVKEHEIRAIIGPNGAGKSSMLNCINGVYS
CCCCHHHEEEEECCCEEECCCHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCC
PQQGAITFRGQTFKHMNSHQVAVMGVARTFQNLALFKGMSVLDNIMSGRNLKIKSNLLLQ
CCCCCEEECCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
ALRIGPAEKEEIMHREAVEKIIDFLEIQAFRKTPVGQLPYGLQKRVDLGRALAMEPQVLL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
LDEPMAGMNVEEKQDMCRFVLDVNDEFGTTVVLIEHDMGVVMDISDRVVVLDYGKKIGDG
EECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCC
TPDEVRNNPDVIRAYLGVEH
CHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MHKKIGDVILDVSNISLSFGGVKALSDISFNVKEHEIRAIIGPNGAGKSSMLNCINGVYS
CCCCHHHEEEEECCCEEECCCHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCC
PQQGAITFRGQTFKHMNSHQVAVMGVARTFQNLALFKGMSVLDNIMSGRNLKIKSNLLLQ
CCCCCEEECCCHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
ALRIGPAEKEEIMHREAVEKIIDFLEIQAFRKTPVGQLPYGLQKRVDLGRALAMEPQVLL
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
LDEPMAGMNVEEKQDMCRFVLDVNDEFGTTVVLIEHDMGVVMDISDRVVVLDYGKKIGDG
EECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCCCC
TPDEVRNNPDVIRAYLGVEH
CHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]