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The map label for this gene is mcl2 [H]

Identifier: 91786486

GI number: 91786486

Start: 583862

End: 584755

Strand: Reverse

Name: mcl2 [H]

Synonym: Bpro_0580

Alternate gene names: 91786486

Gene position: 584755-583862 (Counterclockwise)

Preceding gene: 91786487

Following gene: 91786485

Centisome position: 11.24

GC content: 73.38

Gene sequence:

>894_bases
ATGACCACCGTCCCGGCGCCCGCCCTCGCAACCACTATAGCTATCGCACCCGCAGTGGCGCGCGCGCACGCCCTGCTTTT
TGTGCCCGCCAACCGGCCGGAGCGCATTGCCAAGGCGCTGGCCAGCGGCGCCGGTGCGGTGATCGTGGACCTGGAAGACG
CCGTGCCGCCGGATGCCAAGGCAACCGCCCGCGACCAGCTGGTGCAGGTGTTCTCTAGCCTGGGCGCGGCGGAACGCGCG
CGCCTGCTGGTACGCATCAACGCCGGCAGCACGCCCTGGCATGCCGGCGATTTGGCACTGGTGGGCCGGCTGGCCGCGCA
GGGCCTGGCTGCCGTGATGGTGCCCAAGGCCGAATCCGCCGCCGACCTCGCGCAGGTGGCCGCCGCGGTTGGCCCGGCGT
GCGGCCTGCTGCCGCTCGTGGAGTCGGCCGCCGGACTGGCGGCCGCGGATGCGCTGGCGCACAGCCCGCAGGTCGTCCGG
CTGGCGTTTGGCAACCTCGACTTTCAGGCCGACCTGGGGCTGGCCTGCGAACCGGACGAGGCCGAGCTGGTGTCGGTACG
CCTGGCCCTGGTGCTGGCATCGCGCCGCGCCGGACTGGCGGCGCCGGTCGATGGCGTCACCCCCGACATCGGCGACCTCG
CAGCGCTGCAGGCAACCGCTGCCCGCAGCCGGCGCGGCGGCTTTGGCGCCAAGCTGTGCATTCACCCTGCCCAGGTGGCC
CCGGTCAATGCGGCGCTGGCGCCCAGCGCGGCCGAGCTGGACTGGGCGCGTAGGGTCATTGAAGGCAATGACAGCGAGCG
CGGCGGCGTGTTCGTGCTGGATGGCCGGATGGTCGACGCCCCGGTGGTGCGGCGGGCCCGCCAGCTGCTGGCGCAGGCTC
CCGGTAATCCATGA

Upstream 100 bases:

>100_bases
ACAGCAGTGCCGCCGCGCCCGCCGACATGCAGGCAGCCATCGATGGCCTGTGGGCGGTGGCGCAGTGCCCGCGGCTCGGG
CCGCTGCTCGGAGGTGTGGC

Downstream 100 bases:

>100_bases
ATCGATGAACCGGCGGACCGTCTCTGGCAAGGGATCAGGCAAGGGGTTTTTCCGATTGCGCAGTCTGGCCTCAGCGCCCA
AGATGAGATCACAGGGTGGC

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTTVPAPALATTIAIAPAVARAHALLFVPANRPERIAKALASGAGAVIVDLEDAVPPDAKATARDQLVQVFSSLGAAERA
RLLVRINAGSTPWHAGDLALVGRLAAQGLAAVMVPKAESAADLAQVAAAVGPACGLLPLVESAAGLAAADALAHSPQVVR
LAFGNLDFQADLGLACEPDEAELVSVRLALVLASRRAGLAAPVDGVTPDIGDLAALQATAARSRRGGFGAKLCIHPAQVA
PVNAALAPSAAELDWARRVIEGNDSERGGVFVLDGRMVDAPVVRRARQLLAQAPGNP

Sequences:

>Translated_297_residues
MTTVPAPALATTIAIAPAVARAHALLFVPANRPERIAKALASGAGAVIVDLEDAVPPDAKATARDQLVQVFSSLGAAERA
RLLVRINAGSTPWHAGDLALVGRLAAQGLAAVMVPKAESAADLAQVAAAVGPACGLLPLVESAAGLAAADALAHSPQVVR
LAFGNLDFQADLGLACEPDEAELVSVRLALVLASRRAGLAAPVDGVTPDIGDLAALQATAARSRRGGFGAKLCIHPAQVA
PVNAALAPSAAELDWARRVIEGNDSERGGVFVLDGRMVDAPVVRRARQLLAQAPGNP
>Mature_296_residues
TTVPAPALATTIAIAPAVARAHALLFVPANRPERIAKALASGAGAVIVDLEDAVPPDAKATARDQLVQVFSSLGAAERAR
LLVRINAGSTPWHAGDLALVGRLAAQGLAAVMVPKAESAADLAQVAAAVGPACGLLPLVESAAGLAAADALAHSPQVVRL
AFGNLDFQADLGLACEPDEAELVSVRLALVLASRRAGLAAPVDGVTPDIGDLAALQATAARSRRGGFGAKLCIHPAQVAP
VNAALAPSAAELDWARRVIEGNDSERGGVFVLDGRMVDAPVVRRARQLLAQAPGNP

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=292, Percent_Identity=28.7671232876712, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081764, Length=286, Percent_Identity=32.1678321678322, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17557458, Length=292, Percent_Identity=27.0547945205479, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30022; Mature: 29891

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVPAPALATTIAIAPAVARAHALLFVPANRPERIAKALASGAGAVIVDLEDAVPPDAK
CCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
ATARDQLVQVFSSLGAAERARLLVRINAGSTPWHAGDLALVGRLAAQGLAAVMVPKAESA
HHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHH
ADLAQVAAAVGPACGLLPLVESAAGLAAADALAHSPQVVRLAFGNLDFQADLGLACEPDE
HHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCEEECCCCEECCCH
AELVSVRLALVLASRRAGLAAPVDGVTPDIGDLAALQATAARSRRGGFGAKLCIHPAQVA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHC
PVNAALAPSAAELDWARRVIEGNDSERGGVFVLDGRMVDAPVVRRARQLLAQAPGNP
CCCHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCEECCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTVPAPALATTIAIAPAVARAHALLFVPANRPERIAKALASGAGAVIVDLEDAVPPDAK
CCCCCHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
ATARDQLVQVFSSLGAAERARLLVRINAGSTPWHAGDLALVGRLAAQGLAAVMVPKAESA
HHHHHHHHHHHHHCCHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHH
ADLAQVAAAVGPACGLLPLVESAAGLAAADALAHSPQVVRLAFGNLDFQADLGLACEPDE
HHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCEEECCCCEECCCH
AELVSVRLALVLASRRAGLAAPVDGVTPDIGDLAALQATAARSRRGGFGAKLCIHPAQVA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHC
PVNAALAPSAAELDWARRVIEGNDSERGGVFVLDGRMVDAPVVRRARQLLAQAPGNP
CCCHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCEECCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA