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The map label for this gene is rfaF [H]

Identifier: 91786375

GI number: 91786375

Start: 480181

End: 481428

Strand: Reverse

Name: rfaF [H]

Synonym: Bpro_0465

Alternate gene names: 91786375

Gene position: 481428-480181 (Counterclockwise)

Preceding gene: 91786376

Following gene: 91786374

Centisome position: 9.26

GC content: 65.38

Gene sequence:

>1248_bases
ATGAACATCCAGCTGCAACGATGGATTGACCGCTGGGTCGGCATCCCGTTGTGTGCCGCGGTCTCTGGCCTGAATGCGGT
GACCAGGGCTTTGCGGGGGCCGTCCCGCGAGGTGGCCGCCACCACCGCGCCACGAGCGATCGTGGTGATCCTGCTGTCCG
AAATGGGCAGCCTGGTGCTGGCGCACGATATGTTTGCGCGGCTGAAGCAACGCTATCCCGGCGCCGAGCTGCATGCCCTG
CTGTTCAGGAAGAACCGTGAAATCCTCGACCTCATGAAGGTCGTTGACCCGGCGCATGTGCACACGGTGCATGACAAGTC
GCTGTCGGGCCTGCTGTCCAGCCTGTGGAAAGCGATTGCCGAACTGCGCCGCGCGCGGGTCGACGTGGCGATCGACTGCG
AACTGTTTTCGCGCATCAGCAGCCTGCTGTCGTTTGCCAGTGGTGCGCCTGTTCGCGTGGGCTTTCACCGCCATACGCAG
GAAGGCCTGTACCGCGGCTCGCACATCAACCGGCCCGTGCCCTACAACCCCTATCACCACATCAGCGCGCAGTTTCTGGC
GCTTGCGCGAGCCATCGACTCCACGGCGGTACCGAAATCAAAGCTGCCGATGGTGGGCACCAACAAGCCCCCGCCGCATG
TACAGCTGGACGGCGCCCTGGTGCAGGGTATTGCGCAGCGGCTGGCGCAGGACTTTCCGGCCATCGCCGGCAAGCCGCTG
GTACTCGTCTACCCCGGCGGCGGCATTCTGCCCATCCGCGCGTGGCCACTGGCGTCGTACACCACGCTGTGCGAAGGCCT
GGTGGCCGATGGCTGCGCGGTGGCCGTGATTGGCCTGAAAGACGACCAGGCACTGGCGCAGCAACTGGTCGCCAACGTGA
ACCAGGCCTACCCGGCCGGGCCGGTGATTGACCTGACGGGCTACACCCGATCGATCTCCGAGCTGCTGGCGCTGTTCCAT
GTGGCGCGCCTGCTGGTGACCAACGATGGCGGACCCGGGCAGTTCGCGGCACTGACGCCGATCTGGACGCTGATGCTGTT
CGGGCCTGAAACGCCAGCCCTCTACGCGCCGCACACACCGAAGTGCCATTCGTTTTACAGCCAGTGGCCCTGTTCGCCCT
GCCTGACCGCATACAACCACCGCTCCTCCTACTGCGACGGCGACAACCAGTGCCTCAAGGTCATTGCGCCCGCCACCGTG
CTGGCCAAGGCGCGGGAATGCCTGGCCGAAACTCCGGTGACGGCCTGA

Upstream 100 bases:

>100_bases
CCTGAACCTGATGCAGGAAATCCGCGATGCGCTCGATGCGGGACGGTTCGGCGAGTTCGCCGCGCGCTTCCGGACCGACC
GGCTGAGAGGCGTCTGAGCG

Downstream 100 bases:

>100_bases
TGGGAACACTGCGATCCCTTGCGGAGGCGCTGATCAGCCGCCTGCCACCACGGCTTCAGCCCATGGCGCGCTGGCGTTTC
ATCAAGTTTGGCGCGGTGGG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNIQLQRWIDRWVGIPLCAAVSGLNAVTRALRGPSREVAATTAPRAIVVILLSEMGSLVLAHDMFARLKQRYPGAELHAL
LFRKNREILDLMKVVDPAHVHTVHDKSLSGLLSSLWKAIAELRRARVDVAIDCELFSRISSLLSFASGAPVRVGFHRHTQ
EGLYRGSHINRPVPYNPYHHISAQFLALARAIDSTAVPKSKLPMVGTNKPPPHVQLDGALVQGIAQRLAQDFPAIAGKPL
VLVYPGGGILPIRAWPLASYTTLCEGLVADGCAVAVIGLKDDQALAQQLVANVNQAYPAGPVIDLTGYTRSISELLALFH
VARLLVTNDGGPGQFAALTPIWTLMLFGPETPALYAPHTPKCHSFYSQWPCSPCLTAYNHRSSYCDGDNQCLKVIAPATV
LAKARECLAETPVTA

Sequences:

>Translated_415_residues
MNIQLQRWIDRWVGIPLCAAVSGLNAVTRALRGPSREVAATTAPRAIVVILLSEMGSLVLAHDMFARLKQRYPGAELHAL
LFRKNREILDLMKVVDPAHVHTVHDKSLSGLLSSLWKAIAELRRARVDVAIDCELFSRISSLLSFASGAPVRVGFHRHTQ
EGLYRGSHINRPVPYNPYHHISAQFLALARAIDSTAVPKSKLPMVGTNKPPPHVQLDGALVQGIAQRLAQDFPAIAGKPL
VLVYPGGGILPIRAWPLASYTTLCEGLVADGCAVAVIGLKDDQALAQQLVANVNQAYPAGPVIDLTGYTRSISELLALFH
VARLLVTNDGGPGQFAALTPIWTLMLFGPETPALYAPHTPKCHSFYSQWPCSPCLTAYNHRSSYCDGDNQCLKVIAPATV
LAKARECLAETPVTA
>Mature_415_residues
MNIQLQRWIDRWVGIPLCAAVSGLNAVTRALRGPSREVAATTAPRAIVVILLSEMGSLVLAHDMFARLKQRYPGAELHAL
LFRKNREILDLMKVVDPAHVHTVHDKSLSGLLSSLWKAIAELRRARVDVAIDCELFSRISSLLSFASGAPVRVGFHRHTQ
EGLYRGSHINRPVPYNPYHHISAQFLALARAIDSTAVPKSKLPMVGTNKPPPHVQLDGALVQGIAQRLAQDFPAIAGKPL
VLVYPGGGILPIRAWPLASYTTLCEGLVADGCAVAVIGLKDDQALAQQLVANVNQAYPAGPVIDLTGYTRSISELLALFH
VARLLVTNDGGPGQFAALTPIWTLMLFGPETPALYAPHTPKCHSFYSQWPCSPCLTAYNHRSSYCDGDNQCLKVIAPATV
LAKARECLAETPVTA

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=362, Percent_Identity=23.2044198895028, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 44949; Mature: 44949

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQLQRWIDRWVGIPLCAAVSGLNAVTRALRGPSREVAATTAPRAIVVILLSEMGSLVL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
AHDMFARLKQRYPGAELHALLFRKNREILDLMKVVDPAHVHTVHDKSLSGLLSSLWKAIA
HHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHH
ELRRARVDVAIDCELFSRISSLLSFASGAPVRVGFHRHTQEGLYRGSHINRPVPYNPYHH
HHHHHHCCEEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCHH
ISAQFLALARAIDSTAVPKSKLPMVGTNKPPPHVQLDGALVQGIAQRLAQDFPAIAGKPL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCHHCCCCE
VLVYPGGGILPIRAWPLASYTTLCEGLVADGCAVAVIGLKDDQALAQQLVANVNQAYPAG
EEEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC
PVIDLTGYTRSISELLALFHVARLLVTNDGGPGQFAALTPIWTLMLFGPETPALYAPHTP
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCC
KCHSFYSQWPCSPCLTAYNHRSSYCDGDNQCLKVIAPATVLAKARECLAETPVTA
HHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNIQLQRWIDRWVGIPLCAAVSGLNAVTRALRGPSREVAATTAPRAIVVILLSEMGSLVL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHH
AHDMFARLKQRYPGAELHALLFRKNREILDLMKVVDPAHVHTVHDKSLSGLLSSLWKAIA
HHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHH
ELRRARVDVAIDCELFSRISSLLSFASGAPVRVGFHRHTQEGLYRGSHINRPVPYNPYHH
HHHHHHCCEEECHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCHH
ISAQFLALARAIDSTAVPKSKLPMVGTNKPPPHVQLDGALVQGIAQRLAQDFPAIAGKPL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCHHCCCCE
VLVYPGGGILPIRAWPLASYTTLCEGLVADGCAVAVIGLKDDQALAQQLVANVNQAYPAG
EEEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCC
PVIDLTGYTRSISELLALFHVARLLVTNDGGPGQFAALTPIWTLMLFGPETPALYAPHTP
CEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCC
KCHSFYSQWPCSPCLTAYNHRSSYCDGDNQCLKVIAPATVLAKARECLAETPVTA
HHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]