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The map label for this gene is tgt

Identifier: 91786376

GI number: 91786376

Start: 481432

End: 482628

Strand: Reverse

Name: tgt

Synonym: Bpro_0466

Alternate gene names: 91786376

Gene position: 482628-481432 (Counterclockwise)

Preceding gene: 91786377

Following gene: 91786375

Centisome position: 9.28

GC content: 63.32

Gene sequence:

>1197_bases
ATGCTCGAATTTGAAGTTCTGAAGACCGACGCAAGGACCGGCGAAGGGGCCAATGCCCACCCCGGCTCGCATGCCCGGCG
CGGCCAGTTAACGCTCACCCATGGCGTCGTGCAAACGCCCATCTTCATGCCGGTCGGCACCTATGGCACCGTCAAGGGCG
TGATGCCGCAGTCGCTGCACGAGATGGGCGCCCAGATCATCCTGGGCAACACCTTTCACCTGTGGATGCGCCCCGGGCTG
GACGTGATGAAACAGTTTGGCGGCCTGCACCGGTTCGAAAGCTGGGACAAACCGATACTCACCGACAGCGGCGGCTTTCA
GGTCTGGTCGCTGGGCGACATGCGCAAGATCTCCGAGGAAGGCGTGAAGTTTGCCTCGCCGGTGAATGGCGACAAGCTGT
TCCTGACACCGGAAATCTCCATGCAGATCCAGACGGTGCTCAACAGCGACATCGTGATGCAGTTTGACGAGTGCACCCCT
TACGACACCAAGGGCCACATCACGACCGAAAGCGAGGCGCGCAGCTCGATGGAGCTCAGCCTGCGCTGGGCCAAGCGCTG
CGTGGCCGAGTTTGACAAACTGGAAAACCCCAACGCACTGTTTGGCATTGTGCAGGGCGGCATGTACCAGAACCTGCGCC
ACGAATCGCTGGAGGCCCTGGTAGAACTGGACTTGCCCGGTTACGCGGTGGGCGGCGTCAGCGTGGGCGAGCCCAAGGAA
GAAATGCAGCGCATCATGGCGCACACGCCCCATCGCCTGCCGGCCGACAAGCCGCGCTATCTGATGGGTGTGGGCACGCC
CGAGGATCTGGTGGAGGGTGTGGGCGCCGGCATCGACATGTTTGACTGCGTGATGCCGACACGCAATGCGCGCAACGGCC
ACCTGTTCACGCGCTTTGGCGACCTGAAAATCCGCAACGCGCGCCACAAGGCCGACGAGCAACCGCTGGACACCACCTGC
ACCTGCTACACCTGCAAGGGCCGCACCATGCCCGACGGCAGCACGTCGGGCGGCTTTTCGCGGGCCTACCTGCACCACCT
GGACCGCTGCGGTGAAATGCTGGGCCCGATGCTGGCCAGCATCCACAACCTGCATTACTACCTGAACCTGATGCAGGAAA
TCCGCGATGCGCTCGATGCGGGACGGTTCGGCGAGTTCGCCGCGCGCTTCCGGACCGACCGGCTGAGAGGCGTCTGA

Upstream 100 bases:

>100_bases
ATTGCCGGGCGCTACCGCTTTTTCAGCTATGGCGATGCCATGCTGCTGGCGCGCAAAGCCCCACGCGCAAGCGCCTGAAC
ACACCTGCGACAATCACAAG

Downstream 100 bases:

>100_bases
GCGATGAACATCCAGCTGCAACGATGGATTGACCGCTGGGTCGGCATCCCGTTGTGTGCCGCGGTCTCTGGCCTGAATGC
GGTGACCAGGGCTTTGCGGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLEFEVLKTDARTGEGANAHPGSHARRGQLTLTHGVVQTPIFMPVGTYGTVKGVMPQSLHEMGAQIILGNTFHLWMRPGL
DVMKQFGGLHRFESWDKPILTDSGGFQVWSLGDMRKISEEGVKFASPVNGDKLFLTPEISMQIQTVLNSDIVMQFDECTP
YDTKGHITTESEARSSMELSLRWAKRCVAEFDKLENPNALFGIVQGGMYQNLRHESLEALVELDLPGYAVGGVSVGEPKE
EMQRIMAHTPHRLPADKPRYLMGVGTPEDLVEGVGAGIDMFDCVMPTRNARNGHLFTRFGDLKIRNARHKADEQPLDTTC
TCYTCKGRTMPDGSTSGGFSRAYLHHLDRCGEMLGPMLASIHNLHYYLNLMQEIRDALDAGRFGEFAARFRTDRLRGV

Sequences:

>Translated_398_residues
MLEFEVLKTDARTGEGANAHPGSHARRGQLTLTHGVVQTPIFMPVGTYGTVKGVMPQSLHEMGAQIILGNTFHLWMRPGL
DVMKQFGGLHRFESWDKPILTDSGGFQVWSLGDMRKISEEGVKFASPVNGDKLFLTPEISMQIQTVLNSDIVMQFDECTP
YDTKGHITTESEARSSMELSLRWAKRCVAEFDKLENPNALFGIVQGGMYQNLRHESLEALVELDLPGYAVGGVSVGEPKE
EMQRIMAHTPHRLPADKPRYLMGVGTPEDLVEGVGAGIDMFDCVMPTRNARNGHLFTRFGDLKIRNARHKADEQPLDTTC
TCYTCKGRTMPDGSTSGGFSRAYLHHLDRCGEMLGPMLASIHNLHYYLNLMQEIRDALDAGRFGEFAARFRTDRLRGV
>Mature_398_residues
MLEFEVLKTDARTGEGANAHPGSHARRGQLTLTHGVVQTPIFMPVGTYGTVKGVMPQSLHEMGAQIILGNTFHLWMRPGL
DVMKQFGGLHRFESWDKPILTDSGGFQVWSLGDMRKISEEGVKFASPVNGDKLFLTPEISMQIQTVLNSDIVMQFDECTP
YDTKGHITTESEARSSMELSLRWAKRCVAEFDKLENPNALFGIVQGGMYQNLRHESLEALVELDLPGYAVGGVSVGEPKE
EMQRIMAHTPHRLPADKPRYLMGVGTPEDLVEGVGAGIDMFDCVMPTRNARNGHLFTRFGDLKIRNARHKADEQPLDTTC
TCYTCKGRTMPDGSTSGGFSRAYLHHLDRCGEMLGPMLASIHNLHYYLNLMQEIRDALDAGRFGEFAARFRTDRLRGV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=368, Percent_Identity=43.2065217391304, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1786607, Length=389, Percent_Identity=58.0976863753213, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17544680, Length=370, Percent_Identity=41.6216216216216, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=169, Percent_Identity=28.4023668639053, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920510, Length=368, Percent_Identity=44.5652173913043, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI21355311, Length=143, Percent_Identity=32.8671328671329, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_POLSJ (Q12GB2)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547328.1
- ProteinModelPortal:   Q12GB2
- SMR:   Q12GB2
- STRING:   Q12GB2
- GeneID:   4011489
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0466
- NMPDR:   fig|296591.1.peg.4466
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q12GB2
- ProtClustDB:   PRK00112
- BioCyc:   PSP296591:BPRO_0466-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 44333; Mature: 44333

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFEVLKTDARTGEGANAHPGSHARRGQLTLTHGVVQTPIFMPVGTYGTVKGVMPQSLH
CCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCEEEECCCCCCCCCCCHHHHH
EMGAQIILGNTFHLWMRPGLDVMKQFGGLHRFESWDKPILTDSGGFQVWSLGDMRKISEE
HCCCEEEECCEEEEEECCCHHHHHHHCCCHHHHCCCCCEEECCCCEEEEECCHHHHHHHC
GVKFASPVNGDKLFLTPEISMQIQTVLNSDIVMQFDECTPYDTKGHITTESEARSSMELS
CCEECCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHH
LRWAKRCVAEFDKLENPNALFGIVQGGMYQNLRHESLEALVELDLPGYAVGGVSVGEPKE
HHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHH
EMQRIMAHTPHRLPADKPRYLMGVGTPEDLVEGVGAGIDMFDCVMPTRNARNGHLFTRFG
HHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEC
DLKIRNARHKADEQPLDTTCTCYTCKGRTMPDGSTSGGFSRAYLHHLDRCGEMLGPMLAS
CEEEECCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IHNLHYYLNLMQEIRDALDAGRFGEFAARFRTDRLRGV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEFEVLKTDARTGEGANAHPGSHARRGQLTLTHGVVQTPIFMPVGTYGTVKGVMPQSLH
CCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCEEEECCCCCCCCCCCHHHHH
EMGAQIILGNTFHLWMRPGLDVMKQFGGLHRFESWDKPILTDSGGFQVWSLGDMRKISEE
HCCCEEEECCEEEEEECCCHHHHHHHCCCHHHHCCCCCEEECCCCEEEEECCHHHHHHHC
GVKFASPVNGDKLFLTPEISMQIQTVLNSDIVMQFDECTPYDTKGHITTESEARSSMELS
CCEECCCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCCCEECHHHHHHHHHHH
LRWAKRCVAEFDKLENPNALFGIVQGGMYQNLRHESLEALVELDLPGYAVGGVSVGEPKE
HHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHH
EMQRIMAHTPHRLPADKPRYLMGVGTPEDLVEGVGAGIDMFDCVMPTRNARNGHLFTRFG
HHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEC
DLKIRNARHKADEQPLDTTCTCYTCKGRTMPDGSTSGGFSRAYLHHLDRCGEMLGPMLAS
CEEEECCHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IHNLHYYLNLMQEIRDALDAGRFGEFAARFRTDRLRGV
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA