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The map label for this gene is gsiB [H]

Identifier: 91786336

GI number: 91786336

Start: 440087

End: 441655

Strand: Reverse

Name: gsiB [H]

Synonym: Bpro_0426

Alternate gene names: 91786336

Gene position: 441655-440087 (Counterclockwise)

Preceding gene: 91786337

Following gene: 91786335

Centisome position: 8.49

GC content: 63.22

Gene sequence:

>1569_bases
ATGAAACGTCGCAACCTCATTCAAATGGGTGCCGCTGCCGCCACCGGCCTGACGCTGGCCGGTGTGCCTCTGCATCTGCT
GGCCGCAGGCAAGAAGGGCGGCGTGATCAACGCCGTCGTTCAACCCGAGCCTCCGGGACTGATGATGGGCATCACGCAAA
ACGCCCCCACGCAGCTGATCGCCGGCAACATCTACGAAGGCCTGCTGCGCTACGACGAGAAGATCACTCCGCTGCCTTCG
CTGGCGACCTCGTGGACCTCGAACAAGGAAGGCACGCTGTTCACCTTCAAGCTCAAGCCCGGCGTGCTGTGGCATGACGG
CAAGCCCTTCACGTCGGCGGACGTGGTGTTCTCGGCCGCCGTGTTCCTGCGCAAGACGCACGCACGCCTGCGCGCCAACC
TGGCGGCGATCGAAAGCATCAAGGCCATCGACCCGCTGACGGTCGAGTTCAAGCTCAAGTACCCGTTCGGCCCGTTCCTC
GGCATCTTTGAAGTCGGCAGCATGCCGATGGTGCCCAAGCACATCTACGAAGGCACGGACTTCGCCACCAACCCGGCCAA
CGCCACGCCGATCGGCACCGGCCCCTTCAAGTTCAAGGAGTGGGTCAAGGGCTCCTACATCCAGCTCGTGGCCAACGACA
AGTACCACGTCAAGGACGTGCCGCTGGTCGACAGCATCTACTTCCACATCATTCCCGATGCGGCCTCGCGCGCGGCCGCC
TTCGAGTCGGGCAAGGTCGACCTGGTGCCCGGCGGCGCGGTCGAATACTTCGACGTGCTTCGCCTGTCGAAACTGCCGGG
TGCCGCGGTCACGACCAAGGGCTGGGAGTTCTTTGCGCCGCATGCCTGGCTGTGGATCAACAACCGCAAGGCGCCGATGG
ACAACATCAAGTTCCGTCAGGCCATGATGTTCGCGATCGACCGCGAAGCCATCTGCAAGGTGGCCTGGCAAGGCTTCGCC
AAGCCCGCCACCGGCCCGTTCAACAGCAACATCAAGTTCCACAGCACCGATGTCGCCAAGTACCCGCGCAACGTCGAAAC
CGCCAAAAAGCTGTTGGCCGAAGCCGGCTACAAGGGCGAAACGCTGCGCATGCTGCCGCTGCCCTACGGCGAAACCTGGA
CGCGCTCGGCCGAAATCCTGCGCCAGAACCTGGCCCAGGCCGGCGTGAAGATCGAGATGGTCGCGACCGACGTGGCCGGC
TGGAACCAGAAACTCAACGAGTGGGACTACGACCTCGCCTTCACCTACGTCTACCAGTACGGCGACCCGGCACTCGGCGT
GGCGCGCAACTACACCACGGCCAACATCGCCAAGGGCTCGCCGTTCAACAACGTCGAGGGCTATTCCAACCCGAAGATCG
ACGCGCTGTTCGACGCCGGCGCGAAGGAGAGCGATCCCGAAAAGCGCAGGGCGATCTATCTGCAGGCGCAGAAAATCCTG
CTGGACGAAGTGCCGGTGGCCTGGCTGTACGAAATCAACTTCCCGACGCTCTACCGCACCAAGCTCAGCAACATCGTGAG
CTCGGCCATCGGCCTGAACGACAGCCTGAGCACCGCGTCCATCGCATGA

Upstream 100 bases:

>100_bases
CAGCGGCTGGAGCCTGCCGCGGTCACGTCCTTTCGCGATTGATTTTTCCCTCCCACCCTGCTTCCCTCAACCTCGACAGA
GCCACAAGGAGTTCGTTCTC

Downstream 100 bases:

>100_bases
TGGCGTGCTCGGACCGGCGCAGCGACGGGGGCTGCAAATGAAGCGCACGCAGTTCATGCTGACCCGCGTGCTGCAGGGTC
TGGTGGCGATCCTGCTGATC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKRRNLIQMGAAAATGLTLAGVPLHLLAAGKKGGVINAVVQPEPPGLMMGITQNAPTQLIAGNIYEGLLRYDEKITPLPS
LATSWTSNKEGTLFTFKLKPGVLWHDGKPFTSADVVFSAAVFLRKTHARLRANLAAIESIKAIDPLTVEFKLKYPFGPFL
GIFEVGSMPMVPKHIYEGTDFATNPANATPIGTGPFKFKEWVKGSYIQLVANDKYHVKDVPLVDSIYFHIIPDAASRAAA
FESGKVDLVPGGAVEYFDVLRLSKLPGAAVTTKGWEFFAPHAWLWINNRKAPMDNIKFRQAMMFAIDREAICKVAWQGFA
KPATGPFNSNIKFHSTDVAKYPRNVETAKKLLAEAGYKGETLRMLPLPYGETWTRSAEILRQNLAQAGVKIEMVATDVAG
WNQKLNEWDYDLAFTYVYQYGDPALGVARNYTTANIAKGSPFNNVEGYSNPKIDALFDAGAKESDPEKRRAIYLQAQKIL
LDEVPVAWLYEINFPTLYRTKLSNIVSSAIGLNDSLSTASIA

Sequences:

>Translated_522_residues
MKRRNLIQMGAAAATGLTLAGVPLHLLAAGKKGGVINAVVQPEPPGLMMGITQNAPTQLIAGNIYEGLLRYDEKITPLPS
LATSWTSNKEGTLFTFKLKPGVLWHDGKPFTSADVVFSAAVFLRKTHARLRANLAAIESIKAIDPLTVEFKLKYPFGPFL
GIFEVGSMPMVPKHIYEGTDFATNPANATPIGTGPFKFKEWVKGSYIQLVANDKYHVKDVPLVDSIYFHIIPDAASRAAA
FESGKVDLVPGGAVEYFDVLRLSKLPGAAVTTKGWEFFAPHAWLWINNRKAPMDNIKFRQAMMFAIDREAICKVAWQGFA
KPATGPFNSNIKFHSTDVAKYPRNVETAKKLLAEAGYKGETLRMLPLPYGETWTRSAEILRQNLAQAGVKIEMVATDVAG
WNQKLNEWDYDLAFTYVYQYGDPALGVARNYTTANIAKGSPFNNVEGYSNPKIDALFDAGAKESDPEKRRAIYLQAQKIL
LDEVPVAWLYEINFPTLYRTKLSNIVSSAIGLNDSLSTASIA
>Mature_522_residues
MKRRNLIQMGAAAATGLTLAGVPLHLLAAGKKGGVINAVVQPEPPGLMMGITQNAPTQLIAGNIYEGLLRYDEKITPLPS
LATSWTSNKEGTLFTFKLKPGVLWHDGKPFTSADVVFSAAVFLRKTHARLRANLAAIESIKAIDPLTVEFKLKYPFGPFL
GIFEVGSMPMVPKHIYEGTDFATNPANATPIGTGPFKFKEWVKGSYIQLVANDKYHVKDVPLVDSIYFHIIPDAASRAAA
FESGKVDLVPGGAVEYFDVLRLSKLPGAAVTTKGWEFFAPHAWLWINNRKAPMDNIKFRQAMMFAIDREAICKVAWQGFA
KPATGPFNSNIKFHSTDVAKYPRNVETAKKLLAEAGYKGETLRMLPLPYGETWTRSAEILRQNLAQAGVKIEMVATDVAG
WNQKLNEWDYDLAFTYVYQYGDPALGVARNYTTANIAKGSPFNNVEGYSNPKIDALFDAGAKESDPEKRRAIYLQAQKIL
LDEVPVAWLYEINFPTLYRTKLSNIVSSAIGLNDSLSTASIA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=523, Percent_Identity=26.0038240917782, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1787052, Length=458, Percent_Identity=26.6375545851528, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1787762, Length=506, Percent_Identity=24.901185770751, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789887, Length=476, Percent_Identity=25.2100840336134, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787495, Length=520, Percent_Identity=25.5769230769231, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081878, Length=537, Percent_Identity=23.0912476722533, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789397, Length=524, Percent_Identity=24.0458015267176, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787551, Length=487, Percent_Identity=22.9979466119097, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57416; Mature: 57416

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRNLIQMGAAAATGLTLAGVPLHLLAAGKKGGVINAVVQPEPPGLMMGITQNAPTQLI
CCCCCHHHHCCHHHCCEEECCCCEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCEE
AGNIYEGLLRYDEKITPLPSLATSWTSNKEGTLFTFKLKPGVLWHDGKPFTSADVVFSAA
ECHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHH
VFLRKTHARLRANLAAIESIKAIDPLTVEFKLKYPFGPFLGIFEVGSMPMVPKHIYEGTD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHHHHCCCC
FATNPANATPIGTGPFKFKEWVKGSYIQLVANDKYHVKDVPLVDSIYFHIIPDAASRAAA
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEECCCHHHCEEEEEECCHHHHHHH
FESGKVDLVPGGAVEYFDVLRLSKLPGAAVTTKGWEFFAPHAWLWINNRKAPMDNIKFRQ
HCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCEEECCEEEEEECCCCCCCHHHHHHH
AMMFAIDREAICKVAWQGFAKPATGPFNSNIKFHSTDVAKYPRNVETAKKLLAEAGYKGE
HHHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHCCCCHHHHHHHHHHCCCCCC
TLRMLPLPYGETWTRSAEILRQNLAQAGVKIEMVATDVAGWNQKLNEWDYDLAFTYVYQY
EEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCEEEEEEEEC
GDPALGVARNYTTANIAKGSPFNNVEGYSNPKIDALFDAGAKESDPEKRRAIYLQAQKIL
CCCCHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHEEEEEHHHHH
LDEVPVAWLYEINFPTLYRTKLSNIVSSAIGLNDSLSTASIA
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKRRNLIQMGAAAATGLTLAGVPLHLLAAGKKGGVINAVVQPEPPGLMMGITQNAPTQLI
CCCCCHHHHCCHHHCCEEECCCCEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCEE
AGNIYEGLLRYDEKITPLPSLATSWTSNKEGTLFTFKLKPGVLWHDGKPFTSADVVFSAA
ECHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHH
VFLRKTHARLRANLAAIESIKAIDPLTVEFKLKYPFGPFLGIFEVGSMPMVPKHIYEGTD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHCCCCCCCHHHHHCCCC
FATNPANATPIGTGPFKFKEWVKGSYIQLVANDKYHVKDVPLVDSIYFHIIPDAASRAAA
CCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEECCCHHHCEEEEEECCHHHHHHH
FESGKVDLVPGGAVEYFDVLRLSKLPGAAVTTKGWEFFAPHAWLWINNRKAPMDNIKFRQ
HCCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCEEECCEEEEEECCCCCCCHHHHHHH
AMMFAIDREAICKVAWQGFAKPATGPFNSNIKFHSTDVAKYPRNVETAKKLLAEAGYKGE
HHHHHHCHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHCCCCHHHHHHHHHHCCCCCC
TLRMLPLPYGETWTRSAEILRQNLAQAGVKIEMVATDVAGWNQKLNEWDYDLAFTYVYQY
EEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHCCCCCCCEEEEEEEEC
GDPALGVARNYTTANIAKGSPFNNVEGYSNPKIDALFDAGAKESDPEKRRAIYLQAQKIL
CCCCHHHCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHEEEEEHHHHH
LDEVPVAWLYEINFPTLYRTKLSNIVSSAIGLNDSLSTASIA
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA