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The map label for this gene is bioA [C]

Identifier: 91786331

GI number: 91786331

Start: 434182

End: 435552

Strand: Reverse

Name: bioA [C]

Synonym: Bpro_0421

Alternate gene names: 91786331

Gene position: 435552-434182 (Counterclockwise)

Preceding gene: 91786332

Following gene: 91786330

Centisome position: 8.38

GC content: 66.45

Gene sequence:

>1371_bases
ATGAACGCATTTACCCATGCAGAGAACGCTGCGCACGACATTGCGGCGCATCTCCATCCCTTCACCAATCTCGCCACCCA
TCCGCAAGTCGGTCCGCTGGTGATCCAGCGCGGCGACGGCATCTTTGTCGAAGACGACCAGGGCCGCCGTTATCTCGAAG
CGATGTCGGGCCTGTGGTGCGCATCGCTGGGCTTCAGCAATGCACGGCTGGCCAAGGCCGGCTGCGACGCGTTGCATGCG
CTGCCCTACTACCACACCTTCAACCATCGCTCGAATCCGGCCGCCATCGAACTGGCGCAAAGGCTGCTGGACATCGCACC
GGTACCGATGTCCAAGGTGTTCTTCGCCAACTCGGGTTCCGAGGCCAACGACACCGCGGTCAAGCTGGTCTGGTACTACC
ACAACGCCATCGGCAAGCCGGACAAGAAAAAGATCATCGCGCGCAAGAACGCCTACCATGGCGTCACGGTGGCGGCGGCC
AGCCTCAGCGGCCTGGCGCCCAACCACCGCGACTTCGACCTGCCGATAGCCCGCATCCTGCACGTCGACTGCCCGCACCA
CTACCGCTACGGTTTGCCGGGTGAGACCGAGGAAGCCTTCGCCACCCGGCTGGCCGAAGCGCTGGAGCAGCGCATCCTCG
CGGAAGGCCCGGACACCGTGGCCGCCTTTTTCGCCGAGCCGGTGATGGGCGCCGGCGGCGTGATCGTGCCGCCAGCCAGC
TACTTCGAAAAGATCCAGGCGGTGCTGCGCAAGTACGAGGTGCTGCTGGTGGCTGACGAGGTGATCTGCGGCTTCTGCCG
CACCGGCGAGATGTTCGGCTCCACCACCTTCGGCATGCAGCCCGACATCCTGACCTCGGCCAAGGCCCTGTCCTCGGGCT
ACATGCCGATCTCGGCCGTGATGGTGTCCGGCAAGGTGCATGCGGCCGTGGCGGCCAACAGCGGGAAGATCGGCACCTTC
GGCCACGGCTTCACCTACTCCGGCCATCCGGTGGCCGCCGCTGTGGCGCTTGAGACGCTCAAGGTGTATGAAGATGAACA
GCTGCTCGCGCATGTGCAGTCGCTGGCGCCGCAGTTCCAGCGGGGCCTGCAGCGCCATGCGTCGCGCCCGCTGGTGGGCG
AGGTGCGCGGCGTGGGCCTGATCGGTGCCATCGAACTGGTGGCGGACCCTGCCAGCCGCACACCGTTCGATCCGGCGAAG
AAGGTCGGCGCGCGCCTGGCCGAACTGGCGCTGGAGCAGGGCCTGATCGTGCGCGCCATGGGTGACGCGGTGGCGTTCTG
CCCGCCGCTGATCATCACGGCCGCACAGGTCGACGAGATGTTTGCCCGCTTCGACCGGGCCATGGTCCGGCTGGAGCAGG
AGCAGCCGTGA

Upstream 100 bases:

>100_bases
GGGTCAGCGCCCGGTCAACCTGGTTGACCTGGCGCGCGGCTACTGAGCGGCGGCGCACCAGCCTGACTTCACTGACTTTA
TCTTGCAACCGGCCCCATCC

Downstream 100 bases:

>100_bases
TGCAGGCCTTCTTTGCCGACGACCAGCTGCTGCACGACCCGCAGCAGTTCATGCGGCTGGGCCGGATCTGCAAGCCGACC
GACCTGCCGGCCCGGGCCCA

Product: aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNAFTHAENAAHDIAAHLHPFTNLATHPQVGPLVIQRGDGIFVEDDQGRRYLEAMSGLWCASLGFSNARLAKAGCDALHA
LPYYHTFNHRSNPAAIELAQRLLDIAPVPMSKVFFANSGSEANDTAVKLVWYYHNAIGKPDKKKIIARKNAYHGVTVAAA
SLSGLAPNHRDFDLPIARILHVDCPHHYRYGLPGETEEAFATRLAEALEQRILAEGPDTVAAFFAEPVMGAGGVIVPPAS
YFEKIQAVLRKYEVLLVADEVICGFCRTGEMFGSTTFGMQPDILTSAKALSSGYMPISAVMVSGKVHAAVAANSGKIGTF
GHGFTYSGHPVAAAVALETLKVYEDEQLLAHVQSLAPQFQRGLQRHASRPLVGEVRGVGLIGAIELVADPASRTPFDPAK
KVGARLAELALEQGLIVRAMGDAVAFCPPLIITAAQVDEMFARFDRAMVRLEQEQP

Sequences:

>Translated_456_residues
MNAFTHAENAAHDIAAHLHPFTNLATHPQVGPLVIQRGDGIFVEDDQGRRYLEAMSGLWCASLGFSNARLAKAGCDALHA
LPYYHTFNHRSNPAAIELAQRLLDIAPVPMSKVFFANSGSEANDTAVKLVWYYHNAIGKPDKKKIIARKNAYHGVTVAAA
SLSGLAPNHRDFDLPIARILHVDCPHHYRYGLPGETEEAFATRLAEALEQRILAEGPDTVAAFFAEPVMGAGGVIVPPAS
YFEKIQAVLRKYEVLLVADEVICGFCRTGEMFGSTTFGMQPDILTSAKALSSGYMPISAVMVSGKVHAAVAANSGKIGTF
GHGFTYSGHPVAAAVALETLKVYEDEQLLAHVQSLAPQFQRGLQRHASRPLVGEVRGVGLIGAIELVADPASRTPFDPAK
KVGARLAELALEQGLIVRAMGDAVAFCPPLIITAAQVDEMFARFDRAMVRLEQEQP
>Mature_456_residues
MNAFTHAENAAHDIAAHLHPFTNLATHPQVGPLVIQRGDGIFVEDDQGRRYLEAMSGLWCASLGFSNARLAKAGCDALHA
LPYYHTFNHRSNPAAIELAQRLLDIAPVPMSKVFFANSGSEANDTAVKLVWYYHNAIGKPDKKKIIARKNAYHGVTVAAA
SLSGLAPNHRDFDLPIARILHVDCPHHYRYGLPGETEEAFATRLAEALEQRILAEGPDTVAAFFAEPVMGAGGVIVPPAS
YFEKIQAVLRKYEVLLVADEVICGFCRTGEMFGSTTFGMQPDILTSAKALSSGYMPISAVMVSGKVHAAVAANSGKIGTF
GHGFTYSGHPVAAAVALETLKVYEDEQLLAHVQSLAPQFQRGLQRHASRPLVGEVRGVGLIGAIELVADPASRTPFDPAK
KVGARLAELALEQGLIVRAMGDAVAFCPPLIITAAQVDEMFARFDRAMVRLEQEQP

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI24119277, Length=435, Percent_Identity=30.3448275862069, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI37574042, Length=434, Percent_Identity=29.2626728110599, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI226442705, Length=434, Percent_Identity=28.8018433179723, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4557809, Length=430, Percent_Identity=28.1395348837209, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI13994255, Length=443, Percent_Identity=28.2167042889391, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI226442709, Length=403, Percent_Identity=29.5285359801489, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI284507298, Length=343, Percent_Identity=29.7376093294461, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1786991, Length=445, Percent_Identity=29.2134831460674, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789759, Length=418, Percent_Identity=30.1435406698565, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788044, Length=415, Percent_Identity=30.3614457831325, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693181, Length=418, Percent_Identity=29.1866028708134, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1789016, Length=428, Percent_Identity=29.9065420560748, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787560, Length=411, Percent_Identity=27.9805352798054, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71992977, Length=445, Percent_Identity=27.8651685393258, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32564660, Length=446, Percent_Identity=29.1479820627803, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI25144271, Length=415, Percent_Identity=27.9518072289157, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=222, Percent_Identity=32.4324324324324, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6323470, Length=415, Percent_Identity=30.3614457831325, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324432, Length=437, Percent_Identity=27.9176201372998, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324386, Length=471, Percent_Identity=27.3885350318471, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321456, Length=290, Percent_Identity=24.4827586206897, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161085790, Length=452, Percent_Identity=31.1946902654867, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28574759, Length=456, Percent_Identity=31.5789473684211, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21357415, Length=423, Percent_Identity=29.3144208037825, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI21356575, Length=435, Percent_Identity=28.9655172413793, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI281366494, Length=364, Percent_Identity=25, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667139, Length=364, Percent_Identity=25, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667143, Length=364, Percent_Identity=25, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 49123; Mature: 49123

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFTHAENAAHDIAAHLHPFTNLATHPQVGPLVIQRGDGIFVEDDQGRRYLEAMSGLWC
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHCCCHH
ASLGFSNARLAKAGCDALHALPYYHTFNHRSNPAAIELAQRLLDIAPVPMSKVFFANSGS
HHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHEEEECCCC
EANDTAVKLVWYYHNAIGKPDKKKIIARKNAYHGVTVAAASLSGLAPNHRDFDLPIARIL
CCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHCCCCCCCCCCCHHHHHE
HVDCPHHYRYGLPGETEEAFATRLAEALEQRILAEGPDTVAAFFAEPVMGAGGVIVPPAS
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCHH
YFEKIQAVLRKYEVLLVADEVICGFCRTGEMFGSTTFGMQPDILTSAKALSSGYMPISAV
HHHHHHHHHHHHHEEEEEHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
MVSGKVHAAVAANSGKIGTFGHGFTYSGHPVAAAVALETLKVYEDEQLLAHVQSLAPQFQ
EECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGLQRHASRPLVGEVRGVGLIGAIELVADPASRTPFDPAKKVGARLAELALEQGLIVRAM
HHHHHHCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEC
GDAVAFCPPLIITAAQVDEMFARFDRAMVRLEQEQP
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAFTHAENAAHDIAAHLHPFTNLATHPQVGPLVIQRGDGIFVEDDQGRRYLEAMSGLWC
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHCCCHH
ASLGFSNARLAKAGCDALHALPYYHTFNHRSNPAAIELAQRLLDIAPVPMSKVFFANSGS
HHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCCHHEEEECCCC
EANDTAVKLVWYYHNAIGKPDKKKIIARKNAYHGVTVAAASLSGLAPNHRDFDLPIARIL
CCCCCEEEEEEEEHHHCCCCHHHHHHHHCCCCCCCEEEHHHHHCCCCCCCCCCCHHHHHE
HVDCPHHYRYGLPGETEEAFATRLAEALEQRILAEGPDTVAAFFAEPVMGAGGVIVPPAS
ECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCHH
YFEKIQAVLRKYEVLLVADEVICGFCRTGEMFGSTTFGMQPDILTSAKALSSGYMPISAV
HHHHHHHHHHHHHEEEEEHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
MVSGKVHAAVAANSGKIGTFGHGFTYSGHPVAAAVALETLKVYEDEQLLAHVQSLAPQFQ
EECCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RGLQRHASRPLVGEVRGVGLIGAIELVADPASRTPFDPAKKVGARLAELALEQGLIVRAM
HHHHHHCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEC
GDAVAFCPPLIITAAQVDEMFARFDRAMVRLEQEQP
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]