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The map label for this gene is pilT [H]

Identifier: 91786306

GI number: 91786306

Start: 408468

End: 409511

Strand: Reverse

Name: pilT [H]

Synonym: Bpro_0396

Alternate gene names: 91786306

Gene position: 409511-408468 (Counterclockwise)

Preceding gene: 91786308

Following gene: 91786305

Centisome position: 7.87

GC content: 61.4

Gene sequence:

>1044_bases
ATGGACATCACGCAGCTGCTGGCCTTTAGCGTCAAAAACAAGGCGTCCGACTTGCATCTGTCGGCAGGTTTGCCGCCCAT
GATCCGCGTTCATGGCGACGTGCGGCGCATCAACATCGACCCGCTGGACCACAAGCAGGTGCATGCCATGGTGTACGACA
TCATGAACGACACCCAGCGCAAGAACTATGAAGAGTTCCTGGAGGTGGACTTCTCCTTTGAGATTGACGGACTGGCACGC
TTTCGCGTCAATGCCTTCAACCAGAACCGGGGTGCCGGTGCCGTGCTACGAACCATTCCCTCCAAAATCCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATCCTTGGCGACCTGGCGCTCAAGCCGCGCGGCATGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTTCCTGAATGAAACCGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTTGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGCCCGCACACGCTGAGCTTCAACGC
CGCTCTCAAGTCCGCCCTGCGCGAAGACCCGGACGCGATTCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CCATGTCGGCCGCAGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACGTCAAGCGCCGCCAAGACGATTGACCGCATC
ATCGACGTGTTCCCGGCCGAGGAAAAGGAAATGATCCGCTCCATGCTGTCGGAGTCGCTGCAGGCCGTCATTTCGCAAAC
GCTGTGCAAGACCAAGGACGGCGCGGGCCGCGTGGCCGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGTGAGGCGAAGGTCGCGCAGATGTACTCGTCCATCCAGACCGGCAACAGCGTGGGCATGCAAACGCTGGACCAGAAC
CTGACCGAGCTGGTCAAGCGCAACGTGATTTCGCCCGCGGAAGCGCGCGGCAAGGCGAAAATACCGGAAAACTTTCCAGG
CTAG

Upstream 100 bases:

>100_bases
GTGGTAATCCGGTCACGGACAAGCTGGAGGTTGCGCACAATCATGGTCATAATCGAACTGTAAACTCAGATTCAACCTCG
ACTTCCAAGGATCCCCCGGG

Downstream 100 bases:

>100_bases
ATATATGGCCCCCATGCTTGTCACTTCGTATGCCTACGGCAGTGCGCTGCCCCTTTCACTACAAAAGGGGACTGCGCTTG
CTTGGGGCGGCCCGGCGCTG

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKQVHAMVYDIMNDTQRKNYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAGAVLRTIPSKILTLEQLNAPKILGDLALKPRGMVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNAALKSALREDPDAILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGAGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTELVKRNVISPAEARGKAKIPENFPG

Sequences:

>Translated_347_residues
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKQVHAMVYDIMNDTQRKNYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAGAVLRTIPSKILTLEQLNAPKILGDLALKPRGMVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNAALKSALREDPDAILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGAGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTELVKRNVISPAEARGKAKIPENFPG
>Mature_347_residues
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKQVHAMVYDIMNDTQRKNYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAGAVLRTIPSKILTLEQLNAPKILGDLALKPRGMVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNAALKSALREDPDAILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGAGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTELVKRNVISPAEARGKAKIPENFPG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=320, Percent_Identity=47.5, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI1789723, Length=283, Percent_Identity=35.6890459363958, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786296, Length=219, Percent_Identity=37.8995433789954, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38139; Mature: 38139

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKQVHAMVYDIMNDTQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KNYEEFLEVDFSFEIDGLARFRVNAFNQNRGAGAVLRTIPSKILTLEQLNAPKILGDLAL
CCHHHHHHCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCC
KPRGMVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVEDPIEFVHESKKCLINQREVGP
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCC
HTLSFNAALKSALREDPDAILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
CEEEHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGAGRVAAHEIMLGTSAIRNLIREAKVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QMYSSIQTGNSVGMQTLDQNLTELVKRNVISPAEARGKAKIPENFPG
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKQVHAMVYDIMNDTQR
CCHHHHHHHHHCCCCCCEEECCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHH
KNYEEFLEVDFSFEIDGLARFRVNAFNQNRGAGAVLRTIPSKILTLEQLNAPKILGDLAL
CCHHHHHHCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCC
KPRGMVLVTGPTGSGKSTTLAAMVNFLNETEYGHILTVEDPIEFVHESKKCLINQREVGP
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCC
HTLSFNAALKSALREDPDAILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
CEEEHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHH
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGAGRVAAHEIMLGTSAIRNLIREAKVA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QMYSSIQTGNSVGMQTLDQNLTELVKRNVISPAEARGKAKIPENFPG
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]