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The map label for this gene is pilT [H]

Identifier: 91786305

GI number: 91786305

Start: 407163

End: 408299

Strand: Reverse

Name: pilT [H]

Synonym: Bpro_0395

Alternate gene names: 91786305

Gene position: 408299-407163 (Counterclockwise)

Preceding gene: 91786306

Following gene: 91786304

Centisome position: 7.85

GC content: 60.25

Gene sequence:

>1137_bases
ATGGAGCGCGATCAAGCCAGTAAATTCATCCACGACTTGCTGAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACCGGCGACTTTCCGCCGGCCATGAAGGTGGACGGCAAAATAACCAAAGTCTCGCCACAGCCGCTCAATGCCGGCCACA
CGCTGGCCCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCGGAGTTTGAACGCACCAAGGAATGCAATTTCGCCATT
TCGCCGCCAGGCGTGGGGCGCTTTCGCGTAAACGCCTTCATCCAGCAGGGCAAGGTCGGCATGGTGATGCGGACGATCCC
GGCCGTGCTGCCCACGATTGACGGGCTGGGCGTGCCCCAGGTGCTCAAGGATGTGGTGATGAGCAAGCGGGGGCTGACGA
TTCTCGTCGGCGCCACCGGTTCGGGCAAGTCCACCACACTGGCGGCCATGGTGGACTGGCGCAACGAACAGTCATTCGGC
CACATCATCACGATTGAAGACCCGGTGGAGTTTGTCCATGCGCACAAGAACTGCGTGATCACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGAAGCGGCGCTGAAAAACACCCTGCGCCAGGCGCCTGATGTGATCCTGATGGGCGAGATCCGTG
ACCGTGAAACCATGGAGCATGCCATCGCCTTTGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCGTGATCAACTTCTTCCCCGAAGAACGCCGTACCCAGTTGCTGATGGATTTGTCGCTCAACCT
CAAGGCCATGATTTCGCAGCGCCTGGTCCCCAAGCAGGATGGCAAGGGCCGCTTTGCGGCGGTCGAGGTCATGCTCAATT
CACCGCTGATCTCCGACCTGATCTTCAAGGGCGATGTTTCCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTCGGC
ATGCAGACTTTCGATCAGGCGCTGTACGACGCCTTTGAAAGCCACTTCATCACCTACGAGGATGCACTGCGCAACGCCGA
TTCGGTGAACGACCTGCGTCTGCAGATCAAGCTCAATTCGCAGCGCGCCAAGTCGACCGACCTTGCCGCGGGCACCGAGA
GTTTCGCCATCGTATAA

Upstream 100 bases:

>100_bases
GGACTGCGCTTGCTTGGGGCGGCCCGGCGCTGCGTTCTCTCGCCGCCACGCTTGTCACTTCTTCGGCGGCGACTGAATCC
ACTTAACAGGAGGTGCTCTC

Downstream 100 bases:

>100_bases
CCGTCCGGGCTGAGCCTGTCGAGGGCCCCAACCTCCCAACGCCTGTCGACAAGCTCAGGGCAAAAGGACAGCAGAGTCAA
CACGCCGTGAGCAGCATCAA

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MERDQASKFIHDLLKLMVSRNGSDLFITGDFPPAMKVDGKITKVSPQPLNAGHTLALARAIMNDKQAAEFERTKECNFAI
SPPGVGRFRVNAFIQQGKVGMVMRTIPAVLPTIDGLGVPQVLKDVVMSKRGLTILVGATGSGKSTTLAAMVDWRNEQSFG
HIITIEDPVEFVHAHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDLIFKGDVSEIKEIMKKSRNLG
MQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNSQRAKSTDLAAGTESFAIV

Sequences:

>Translated_378_residues
MERDQASKFIHDLLKLMVSRNGSDLFITGDFPPAMKVDGKITKVSPQPLNAGHTLALARAIMNDKQAAEFERTKECNFAI
SPPGVGRFRVNAFIQQGKVGMVMRTIPAVLPTIDGLGVPQVLKDVVMSKRGLTILVGATGSGKSTTLAAMVDWRNEQSFG
HIITIEDPVEFVHAHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDLIFKGDVSEIKEIMKKSRNLG
MQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNSQRAKSTDLAAGTESFAIV
>Mature_378_residues
MERDQASKFIHDLLKLMVSRNGSDLFITGDFPPAMKVDGKITKVSPQPLNAGHTLALARAIMNDKQAAEFERTKECNFAI
SPPGVGRFRVNAFIQQGKVGMVMRTIPAVLPTIDGLGVPQVLKDVVMSKRGLTILVGATGSGKSTTLAAMVDWRNEQSFG
HIITIEDPVEFVHAHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDLIFKGDVSEIKEIMKKSRNLG
MQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNSQRAKSTDLAAGTESFAIV

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=319, Percent_Identity=37.6175548589342, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1786296, Length=356, Percent_Identity=29.7752808988764, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=40.5063291139241, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 41805; Mature: 41805

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDQASKFIHDLLKLMVSRNGSDLFITGDFPPAMKVDGKITKVSPQPLNAGHTLALARA
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCCEEEEECCCCCCCCHHHHHHHH
IMNDKQAAEFERTKECNFAISPPGVGRFRVNAFIQQGKVGMVMRTIPAVLPTIDGLGVPQ
HHCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHH
VLKDVVMSKRGLTILVGATGSGKSTTLAAMVDWRNEQSFGHIITIEDPVEFVHAHKNCVI
HHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEECCHHHHHHHCCCEEE
TQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
EHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCH
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDL
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHH
IFKGDVSEIKEIMKKSRNLGMQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNS
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEHHHHHCCCCCCCEEEEEEEECC
QRAKSTDLAAGTESFAIV
CCCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MERDQASKFIHDLLKLMVSRNGSDLFITGDFPPAMKVDGKITKVSPQPLNAGHTLALARA
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEECCCEEEEECCCCCCCCHHHHHHHH
IMNDKQAAEFERTKECNFAISPPGVGRFRVNAFIQQGKVGMVMRTIPAVLPTIDGLGVPQ
HHCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHH
VLKDVVMSKRGLTILVGATGSGKSTTLAAMVDWRNEQSFGHIITIEDPVEFVHAHKNCVI
HHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEECCHHHHHHHCCCEEE
TQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFAETGHLCLATLHANSA
EHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEEEECCCH
NQALDRVINFFPEERRTQLLMDLSLNLKAMISQRLVPKQDGKGRFAAVEVMLNSPLISDL
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHH
IFKGDVSEIKEIMKKSRNLGMQTFDQALYDAFESHFITYEDALRNADSVNDLRLQIKLNS
HHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEHHHHHCCCCCCCEEEEEEEECC
QRAKSTDLAAGTESFAIV
CCCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]