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The map label for this gene is appA [H]

Identifier: 91786290

GI number: 91786290

Start: 392541

End: 394241

Strand: Direct

Name: appA [H]

Synonym: Bpro_0380

Alternate gene names: 91786290

Gene position: 392541-394241 (Clockwise)

Preceding gene: 91786288

Following gene: 91786291

Centisome position: 7.55

GC content: 61.38

Gene sequence:

>1701_bases
ATGAGTACCCACACGCTGCCGTCCACGCCCGCCAAACGCCATTTCGCCTGCTCATTGGCTCCTGCATCGGCACTTTCGTT
GGCGCTGGCCGCCACACTTGGCCTGACGGCCACCGTTGCGCTGGCGCAGGAAAAAACGTTGCGTATCGCGATGACTGCCG
CCGATATCCCGCGCACATTGGGTCAGCCCGACCAGGGCTTTGAGGGCAATCGCTTTACAGGTATCCCGATTTACGACTCG
CTCACCCAATGGGATCTTTCCAAGGCCAATGCACCCAGCGTGCTGATCGCCGGGCTCGCGACCTCCTGGGCGGTGGATGC
CAAGGACAAGACCAAGTGGGTCTTCAAGTTGCGCCCTGGCGTCAAGTTCCATGACGGCTCGGCCTTCAATGCCGATGCGG
TGGTGTGGAATGTGGCCAAGGTGTTCGACAAAGAGGCGCCGCAGTTCGACCCGAGCCAGGTGGGGGTGACTGCTTCGCGC
ATGCCAACGCTGCGCAGCGCACGCAAAATTGACGACATGACGGTCGAGTTCACCACCAGTGAGCCCGACTCATTCCTGCC
CATCAATCTGACCAACCTGTTCATGGCCTCGCCCGCCCAGTGGGATGCCAAGCTGAAGGCCGTTCCTGCCAGCGTGACTG
CTCCGGCAGAACGCTCGAAGGCGGCGTGGACCGCCTTTGCTGCTGATCCAGCCGGCTCCGGCCCTTTCAAGGTAACCCGC
TTTGTCGCGCGCGAACGGCTTGAGCTGGCGGCCCATAAAACTTATTGGGATGCCAGGCGCGTGCCCAAAATCGACAAGGT
CGTGATGTTGCCCATGCCCGAGGCCAATGCCCGCACGGCCGCTCTGCTCGGTGGCCAGGTTGACTGGATCGAAGCGCCGG
CACCCGACGCCATGCCGCAGATCAAGCAGCGCGGCTTCACTATCTACTCCAACGCTCAGCCCCACGTCTGGCCGTGGCAG
CTCTCATTCGCCGAGGGATCGCCCTGGCTCGACAAGCGCGTGCGCCAGGCGGCCAATCTTTGCGTGGACCGTGGTGGCCT
GTCGCAACTTCTGGGTGGCATGATGGCCGAACCCAAGGGCACCGTGCCGCCCGGCCACCCGTGGTGGGGCAATCCGAAGT
TCGACATCAAATATGACGTGAAGGGGGCGCAGGCCCTCATGACGCAAGCCGGTTTTTCGGCGGCCAAACCCGTCAAGGTG
AAGGTGCAGATATCAGCCTCGGGCTCGGGTCAGATGCAGCCTCTGCCCATGAACGAGTTCATGCAGCAATCGCTCAAGCA
GTGCTTTTTTGACGTGCAGTTTGATGTGATCGAGTGGAACACGCTGTTCACCAACTGGCGCAAGGGGGCCAAGGATCCCT
CGGCCAACGGGGCCAACGCGGTCAATGTCAGCTTTGCAGCCATGGACCCCTTCTTTGCCATGGTGCGCTTCGTGAGCACC
AAAACCTTCCCGCCAGTTTCAAACAACTGGGGCTACTATGGCAATGCGGATGTCGACAAACTGGTGGCTGACGCGCGTAC
CAGCTTCGATGAAAAGTCGCGCGACGCGGCGCTTGCCAGGCTCCACGCCCATATCGTGGAAGATGCGCCCTTTGTCTGGG
TCGCGCACGACGTCGGTCCGCGCGCCATGTCTGCAAAGGTCAAGAACGTGGTGCAGCCCAAAAGCTGGTTTATTGACATC
GCCACCATGACGATGGACTGA

Upstream 100 bases:

>100_bases
ATAGTCGATTTCATAAAACCCAGAGTGGCATAAACATTGCTCACCGATTGGGGTGTCTCCAAACCCGCTCCTCAACCTCG
CAAAGCTACAAGGTGATCCC

Downstream 100 bases:

>100_bases
AACCGGTCCCGACCCCGCCCAGCCAGTCGTCCAGCAGCTTCCACACCCGCGAATCACCTCATGCAGCGTTACCTGTTAAG
CCGTGTTTTATATGCCTTGC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSTHTLPSTPAKRHFACSLAPASALSLALAATLGLTATVALAQEKTLRIAMTAADIPRTLGQPDQGFEGNRFTGIPIYDS
LTQWDLSKANAPSVLIAGLATSWAVDAKDKTKWVFKLRPGVKFHDGSAFNADAVVWNVAKVFDKEAPQFDPSQVGVTASR
MPTLRSARKIDDMTVEFTTSEPDSFLPINLTNLFMASPAQWDAKLKAVPASVTAPAERSKAAWTAFAADPAGSGPFKVTR
FVARERLELAAHKTYWDARRVPKIDKVVMLPMPEANARTAALLGGQVDWIEAPAPDAMPQIKQRGFTIYSNAQPHVWPWQ
LSFAEGSPWLDKRVRQAANLCVDRGGLSQLLGGMMAEPKGTVPPGHPWWGNPKFDIKYDVKGAQALMTQAGFSAAKPVKV
KVQISASGSGQMQPLPMNEFMQQSLKQCFFDVQFDVIEWNTLFTNWRKGAKDPSANGANAVNVSFAAMDPFFAMVRFVST
KTFPPVSNNWGYYGNADVDKLVADARTSFDEKSRDAALARLHAHIVEDAPFVWVAHDVGPRAMSAKVKNVVQPKSWFIDI
ATMTMD

Sequences:

>Translated_566_residues
MSTHTLPSTPAKRHFACSLAPASALSLALAATLGLTATVALAQEKTLRIAMTAADIPRTLGQPDQGFEGNRFTGIPIYDS
LTQWDLSKANAPSVLIAGLATSWAVDAKDKTKWVFKLRPGVKFHDGSAFNADAVVWNVAKVFDKEAPQFDPSQVGVTASR
MPTLRSARKIDDMTVEFTTSEPDSFLPINLTNLFMASPAQWDAKLKAVPASVTAPAERSKAAWTAFAADPAGSGPFKVTR
FVARERLELAAHKTYWDARRVPKIDKVVMLPMPEANARTAALLGGQVDWIEAPAPDAMPQIKQRGFTIYSNAQPHVWPWQ
LSFAEGSPWLDKRVRQAANLCVDRGGLSQLLGGMMAEPKGTVPPGHPWWGNPKFDIKYDVKGAQALMTQAGFSAAKPVKV
KVQISASGSGQMQPLPMNEFMQQSLKQCFFDVQFDVIEWNTLFTNWRKGAKDPSANGANAVNVSFAAMDPFFAMVRFVST
KTFPPVSNNWGYYGNADVDKLVADARTSFDEKSRDAALARLHAHIVEDAPFVWVAHDVGPRAMSAKVKNVVQPKSWFIDI
ATMTMD
>Mature_565_residues
STHTLPSTPAKRHFACSLAPASALSLALAATLGLTATVALAQEKTLRIAMTAADIPRTLGQPDQGFEGNRFTGIPIYDSL
TQWDLSKANAPSVLIAGLATSWAVDAKDKTKWVFKLRPGVKFHDGSAFNADAVVWNVAKVFDKEAPQFDPSQVGVTASRM
PTLRSARKIDDMTVEFTTSEPDSFLPINLTNLFMASPAQWDAKLKAVPASVTAPAERSKAAWTAFAADPAGSGPFKVTRF
VARERLELAAHKTYWDARRVPKIDKVVMLPMPEANARTAALLGGQVDWIEAPAPDAMPQIKQRGFTIYSNAQPHVWPWQL
SFAEGSPWLDKRVRQAANLCVDRGGLSQLLGGMMAEPKGTVPPGHPWWGNPKFDIKYDVKGAQALMTQAGFSAAKPVKVK
VQISASGSGQMQPLPMNEFMQQSLKQCFFDVQFDVIEWNTLFTNWRKGAKDPSANGANAVNVSFAAMDPFFAMVRFVSTK
TFPPVSNNWGYYGNADVDKLVADARTSFDEKSRDAALARLHAHIVEDAPFVWVAHDVGPRAMSAKVKNVVQPKSWFIDIA
TMTMD

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=568, Percent_Identity=24.4718309859155, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081878, Length=542, Percent_Identity=22.8782287822878, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787495, Length=558, Percent_Identity=22.2222222222222, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787052, Length=438, Percent_Identity=24.2009132420091, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787551, Length=331, Percent_Identity=25.0755287009063, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61796; Mature: 61665

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTHTLPSTPAKRHFACSLAPASALSLALAATLGLTATVALAQEKTLRIAMTAADIPRTL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEHHHHHHHC
GQPDQGFEGNRFTGIPIYDSLTQWDLSKANAPSVLIAGLATSWAVDAKDKTKWVFKLRPG
CCCCCCCCCCCEECCCCCCCCHHCCCCCCCCCCEEEEECHHHEEECCCCCCEEEEEECCC
VKFHDGSAFNADAVVWNVAKVFDKEAPQFDPSQVGVTASRMPTLRSARKIDDMTVEFTTS
CEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCEEHHHCCHHHHHHCCCCEEEEEECC
EPDSFLPINLTNLFMASPAQWDAKLKAVPASVTAPAERSKAAWTAFAADPAGSGPFKVTR
CCCCEEEEEHHHHEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
FVARERLELAAHKTYWDARRVPKIDKVVMLPMPEANARTAALLGGQVDWIEAPAPDAMPQ
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCEEEECCCCCCCHHH
IKQRGFTIYSNAQPHVWPWQLSFAEGSPWLDKRVRQAANLCVDRGGLSQLLGGMMAEPKG
HHHCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
TVPPGHPWWGNPKFDIKYDVKGAQALMTQAGFSAAKPVKVKVQISASGSGQMQPLPMNEF
CCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHH
MQQSLKQCFFDVQFDVIEWNTLFTNWRKGAKDPSANGANAVNVSFAAMDPFFAMVRFVST
HHHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHH
KTFPPVSNNWGYYGNADVDKLVADARTSFDEKSRDAALARLHAHIVEDAPFVWVAHDVGP
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
RAMSAKVKNVVQPKSWFIDIATMTMD
HHHHHHHHHHCCCCCEEEEEEEEECC
>Mature Secondary Structure 
STHTLPSTPAKRHFACSLAPASALSLALAATLGLTATVALAQEKTLRIAMTAADIPRTL
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEHHHHHHHC
GQPDQGFEGNRFTGIPIYDSLTQWDLSKANAPSVLIAGLATSWAVDAKDKTKWVFKLRPG
CCCCCCCCCCCEECCCCCCCCHHCCCCCCCCCCEEEEECHHHEEECCCCCCEEEEEECCC
VKFHDGSAFNADAVVWNVAKVFDKEAPQFDPSQVGVTASRMPTLRSARKIDDMTVEFTTS
CEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHCCEEHHHCCHHHHHHCCCCEEEEEECC
EPDSFLPINLTNLFMASPAQWDAKLKAVPASVTAPAERSKAAWTAFAADPAGSGPFKVTR
CCCCEEEEEHHHHEECCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHH
FVARERLELAAHKTYWDARRVPKIDKVVMLPMPEANARTAALLGGQVDWIEAPAPDAMPQ
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCEEEECCCCCCCHHH
IKQRGFTIYSNAQPHVWPWQLSFAEGSPWLDKRVRQAANLCVDRGGLSQLLGGMMAEPKG
HHHCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
TVPPGHPWWGNPKFDIKYDVKGAQALMTQAGFSAAKPVKVKVQISASGSGQMQPLPMNEF
CCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHH
MQQSLKQCFFDVQFDVIEWNTLFTNWRKGAKDPSANGANAVNVSFAAMDPFFAMVRFVST
HHHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHH
KTFPPVSNNWGYYGNADVDKLVADARTSFDEKSRDAALARLHAHIVEDAPFVWVAHDVGP
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
RAMSAKVKNVVQPKSWFIDIATMTMD
HHHHHHHHHHCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]