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The map label for this gene is mrdA [H]

Identifier: 91786136

GI number: 91786136

Start: 245135

End: 247210

Strand: Direct

Name: mrdA [H]

Synonym: Bpro_0225

Alternate gene names: 91786136

Gene position: 245135-247210 (Clockwise)

Preceding gene: 91786135

Following gene: 91786141

Centisome position: 4.71

GC content: 65.99

Gene sequence:

>2076_bases
ATGACTGAGCTGCGCAACGTTGAAGCCGATTTGTCGCGCTTTCGCGCGCGCGTGCTGGTCGCGAGCCTGGCCGTGCTCTG
CGCCTTTGGGCTGCTGGCGGCGCGGCTGGTCTACCTGCAGGTGTTCCGCCACACCGACCTGAGCGAGCAGGCCGAAAGCA
ACCGCACGGCCATCGTGCCCATCGTGCCCAACCGCGGGCTGATCCTGGACCGCAACGGCGTGGTGCTGGCCAGCAACTAC
TCGGCCTACACGCTGGAGATCACGCCGTCCAAGGTGGCCAACCTGGAGGAGACCATTGCGGCGCTGGAAAAAGTGGTGGA
CATCCAGCTGCGCGACAAGCGGCGCTTCAAGCGCCTGCGCGAAGAGGCCAAGAATTTCGAGTCGCTGCCCATACGCACCC
GCCTGACCGATGTGGAAGTGGCCCGGTTTGCCGCGCAGCGTTTCCGCTTTCCGGGCGTCGACATCAAGGCGCGGCTGTTC
CGCAGCTACCCGTACGGCGAGCTCGGCAGCCATGTCGTGGGCTACATCGGCCGCATCAACCAGAGCGAAAAAGAGCGTAT
CGACGAAGGCGACGACCAGGGCAACTACCGCGGCACGGACTACATCGGCAAGCTCGGCGTGGAGCAGAGCTTTGAAAAGC
AGCTGCACGGCATCACGGGTGTCGAGCAGGTCGAGACCTCGGCGGGCGGGCGGGCGGTGCGCCGGCTGGCCAGCAACCCG
GCCATCCCCGGCGACACGGTGATGCTGTCCATCGACATCAAGCTGCAAAAGCTGGTGGAAGACATGTTCGGCGATCGGCG
CGGCGCGCTGGTGGCGCTGGACCCGAAAACCGGCGATGTGCTGGCGTTTGTCAGCAAGCCGACGTTCGACCCCAACCTGT
TCGTGGACGGGATTGATGCCGAGAGCTGGGAAGCCCTCAACGAATCGATCGACAAGCCGCTGCTCAACCGGGCGCTGCGC
GGCACCTACCCGCCAGGCTCCACCTATAAACCTTTCATGGCCCTGGCCGCCCTGCAGCTCGGCGCGCGAGCCCCAGGAAC
CCTGATCAACGACCCCGGTTTCTACATGTTCGGCGGCCGCCGTTTCGGCAGCCCCGAGAACGAGAAGGGCGGCATCATGG
ACATGCACCGGGCCATTGTCGAGTCCAGCAATGTTTACTTTTACTCGCTGGCCAACGAAATCGGCGTGGACGCCATGCAT
GACTTTATGAAGCCGCTGGGCTTCGGCCAGATCACCGGCATCGACATCAATGGCGAAGTGCGCGGTGTGCTGCCCAGCCA
GGCCTGGAAGCGCAGCTACTTCAAACGGCCGGAACAGCAGAAATGGTTTGCCGGTGAGACCATTTCGCTCGGCATTGGAC
AGGGCTACAACAGTTTCACCATGCTGCAACTGGCCCAGGCCACCGCTGTGCTGGCCAATAACGGTATCAAGTACAAGCCG
CGCCTGATCATCGGCACGCAGGACCCGGTGACACGGGCGATGCACCCCCTGCCGGTTGCGCCGCCTGAAGACCTGGGTTA
CAAGCCCGAGAACGTGGCGGTGATCCGCAAGGGGCTGGCCGGCGTGGTGCAGGGCGGAACCTCGGCCAGGGTGTTTGCTG
GCGCGGGCTACCTGAGCGGCGGTAAAACCGGCACGGCCCAGGCCGTGGGAGCCCCGAAAGGCAGCAAGTACAACAGCGCC
AAAATGGAAGAACACCAGCGCGACCATGCGCTGTACATTGCCGTTGCCCCCGCCGAGGACCCCCGGATCGCACTGGCGGT
GATCGTGGAAAACGCCGGCTGGGGCGCCGGCGCGGCCGCGCCCATTGCACGCCGGGCCTTTGACTACCTGTTGCTGGGCC
AGTACCCGAGCGAGGAAGACATGGCTGCCGTGCAAAAAGGGCTGGCCGCGGCACCCATTGGCAAGCCGCGCCGCGCGGCC
GACATCGCGGCCTTGCCGGTCGCCGGGGTGCCGGCCATGGAGGCACCCCGGGTGGAGGGGCCCGCGGCGGCCACTGCCGC
CAGGACCGAGGGCGTGGTGCCGGTGGCCGGCAAGCCAGCGGCGTCTGCGCCGGTAGCGGCTACCCTGCCGCGTTAG

Upstream 100 bases:

>100_bases
CCAGTCGGGGCCGCGGAACTGGCTTTGCCAGGCCGCAGGCGCAGCGGCCCCCTCGGGGGGCAGGGAGCACACGTGGTGAG
CGACCGTGGGGGCAATCTTC

Downstream 100 bases:

>100_bases
CTTTTCTGCGGCGGGTCAGCGGGGGCTATTTCAAAAAGGCGTCGAACGCTGTTTTCAGGATCAGCGCGCTGACCACCACA
ATAAACACGCTTCGCACAAA

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MTELRNVEADLSRFRARVLVASLAVLCAFGLLAARLVYLQVFRHTDLSEQAESNRTAIVPIVPNRGLILDRNGVVLASNY
SAYTLEITPSKVANLEETIAALEKVVDIQLRDKRRFKRLREEAKNFESLPIRTRLTDVEVARFAAQRFRFPGVDIKARLF
RSYPYGELGSHVVGYIGRINQSEKERIDEGDDQGNYRGTDYIGKLGVEQSFEKQLHGITGVEQVETSAGGRAVRRLASNP
AIPGDTVMLSIDIKLQKLVEDMFGDRRGALVALDPKTGDVLAFVSKPTFDPNLFVDGIDAESWEALNESIDKPLLNRALR
GTYPPGSTYKPFMALAALQLGARAPGTLINDPGFYMFGGRRFGSPENEKGGIMDMHRAIVESSNVYFYSLANEIGVDAMH
DFMKPLGFGQITGIDINGEVRGVLPSQAWKRSYFKRPEQQKWFAGETISLGIGQGYNSFTMLQLAQATAVLANNGIKYKP
RLIIGTQDPVTRAMHPLPVAPPEDLGYKPENVAVIRKGLAGVVQGGTSARVFAGAGYLSGGKTGTAQAVGAPKGSKYNSA
KMEEHQRDHALYIAVAPAEDPRIALAVIVENAGWGAGAAAPIARRAFDYLLLGQYPSEEDMAAVQKGLAAAPIGKPRRAA
DIAALPVAGVPAMEAPRVEGPAAATAARTEGVVPVAGKPAASAPVAATLPR

Sequences:

>Translated_691_residues
MTELRNVEADLSRFRARVLVASLAVLCAFGLLAARLVYLQVFRHTDLSEQAESNRTAIVPIVPNRGLILDRNGVVLASNY
SAYTLEITPSKVANLEETIAALEKVVDIQLRDKRRFKRLREEAKNFESLPIRTRLTDVEVARFAAQRFRFPGVDIKARLF
RSYPYGELGSHVVGYIGRINQSEKERIDEGDDQGNYRGTDYIGKLGVEQSFEKQLHGITGVEQVETSAGGRAVRRLASNP
AIPGDTVMLSIDIKLQKLVEDMFGDRRGALVALDPKTGDVLAFVSKPTFDPNLFVDGIDAESWEALNESIDKPLLNRALR
GTYPPGSTYKPFMALAALQLGARAPGTLINDPGFYMFGGRRFGSPENEKGGIMDMHRAIVESSNVYFYSLANEIGVDAMH
DFMKPLGFGQITGIDINGEVRGVLPSQAWKRSYFKRPEQQKWFAGETISLGIGQGYNSFTMLQLAQATAVLANNGIKYKP
RLIIGTQDPVTRAMHPLPVAPPEDLGYKPENVAVIRKGLAGVVQGGTSARVFAGAGYLSGGKTGTAQAVGAPKGSKYNSA
KMEEHQRDHALYIAVAPAEDPRIALAVIVENAGWGAGAAAPIARRAFDYLLLGQYPSEEDMAAVQKGLAAAPIGKPRRAA
DIAALPVAGVPAMEAPRVEGPAAATAARTEGVVPVAGKPAASAPVAATLPR
>Mature_690_residues
TELRNVEADLSRFRARVLVASLAVLCAFGLLAARLVYLQVFRHTDLSEQAESNRTAIVPIVPNRGLILDRNGVVLASNYS
AYTLEITPSKVANLEETIAALEKVVDIQLRDKRRFKRLREEAKNFESLPIRTRLTDVEVARFAAQRFRFPGVDIKARLFR
SYPYGELGSHVVGYIGRINQSEKERIDEGDDQGNYRGTDYIGKLGVEQSFEKQLHGITGVEQVETSAGGRAVRRLASNPA
IPGDTVMLSIDIKLQKLVEDMFGDRRGALVALDPKTGDVLAFVSKPTFDPNLFVDGIDAESWEALNESIDKPLLNRALRG
TYPPGSTYKPFMALAALQLGARAPGTLINDPGFYMFGGRRFGSPENEKGGIMDMHRAIVESSNVYFYSLANEIGVDAMHD
FMKPLGFGQITGIDINGEVRGVLPSQAWKRSYFKRPEQQKWFAGETISLGIGQGYNSFTMLQLAQATAVLANNGIKYKPR
LIIGTQDPVTRAMHPLPVAPPEDLGYKPENVAVIRKGLAGVVQGGTSARVFAGAGYLSGGKTGTAQAVGAPKGSKYNSAK
MEEHQRDHALYIAVAPAEDPRIALAVIVENAGWGAGAAAPIARRAFDYLLLGQYPSEEDMAAVQKGLAAAPIGKPRRAAD
IAALPVAGVPAMEAPRVEGPAAATAARTEGVVPVAGKPAASAPVAATLPR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=635, Percent_Identity=39.5275590551181, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1786272, Length=596, Percent_Identity=26.1744966442953, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 74565; Mature: 74434

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELRNVEADLSRFRARVLVASLAVLCAFGLLAARLVYLQVFRHTDLSEQAESNRTAIVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEE
IVPNRGLILDRNGVVLASNYSAYTLEITPSKVANLEETIAALEKVVDIQLRDKRRFKRLR
EECCCCEEEECCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAKNFESLPIRTRLTDVEVARFAAQRFRFPGVDIKARLFRSYPYGELGSHVVGYIGRIN
HHHCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCC
QSEKERIDEGDDQGNYRGTDYIGKLGVEQSFEKQLHGITGVEQVETSAGGRAVRRLASNP
CHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCC
AIPGDTVMLSIDIKLQKLVEDMFGDRRGALVALDPKTGDVLAFVSKPTFDPNLFVDGIDA
CCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCEEECCCC
ESWEALNESIDKPLLNRALRGTYPPGSTYKPFMALAALQLGARAPGTLINDPGFYMFGGR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEEECCE
RFGSPENEKGGIMDMHRAIVESSNVYFYSLANEIGVDAMHDFMKPLGFGQITGIDINGEV
ECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHCHHHHHHHHHHCCCCCEEEEEECCCE
RGVLPSQAWKRSYFKRPEQQKWFAGETISLGIGQGYNSFTMLQLAQATAVLANNGIKYKP
EECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEECC
RLIIGTQDPVTRAMHPLPVAPPEDLGYKPENVAVIRKGLAGVVQGGTSARVFAGAGYLSG
EEEEECCCCHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
GKTGTAQAVGAPKGSKYNSAKMEEHQRDHALYIAVAPAEDPRIALAVIVENAGWGAGAAA
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHH
PIARRAFDYLLLGQYPSEEDMAAVQKGLAAAPIGKPRRAADIAALPVAGVPAMEAPRVEG
HHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
PAAATAARTEGVVPVAGKPAASAPVAATLPR
CCHHHHHHCCCEEECCCCCCCCCCCEECCCC
>Mature Secondary Structure 
TELRNVEADLSRFRARVLVASLAVLCAFGLLAARLVYLQVFRHTDLSEQAESNRTAIVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEE
IVPNRGLILDRNGVVLASNYSAYTLEITPSKVANLEETIAALEKVVDIQLRDKRRFKRLR
EECCCCEEEECCCEEEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAKNFESLPIRTRLTDVEVARFAAQRFRFPGVDIKARLFRSYPYGELGSHVVGYIGRIN
HHHCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCC
QSEKERIDEGDDQGNYRGTDYIGKLGVEQSFEKQLHGITGVEQVETSAGGRAVRRLASNP
CHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCC
AIPGDTVMLSIDIKLQKLVEDMFGDRRGALVALDPKTGDVLAFVSKPTFDPNLFVDGIDA
CCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCCEEECCCC
ESWEALNESIDKPLLNRALRGTYPPGSTYKPFMALAALQLGARAPGTLINDPGFYMFGGR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCEEEECCE
RFGSPENEKGGIMDMHRAIVESSNVYFYSLANEIGVDAMHDFMKPLGFGQITGIDINGEV
ECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHCHHHHHHHHHHCCCCCEEEEEECCCE
RGVLPSQAWKRSYFKRPEQQKWFAGETISLGIGQGYNSFTMLQLAQATAVLANNGIKYKP
EECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEECC
RLIIGTQDPVTRAMHPLPVAPPEDLGYKPENVAVIRKGLAGVVQGGTSARVFAGAGYLSG
EEEEECCCCHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
GKTGTAQAVGAPKGSKYNSAKMEEHQRDHALYIAVAPAEDPRIALAVIVENAGWGAGAAA
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHH
PIARRAFDYLLLGQYPSEEDMAAVQKGLAAAPIGKPRRAADIAALPVAGVPAMEAPRVEG
HHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
PAAATAARTEGVVPVAGKPAASAPVAATLPR
CCHHHHHHCCCEEECCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]