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The map label for this gene is gatB [H]

Identifier: 91786130

GI number: 91786130

Start: 238785

End: 240287

Strand: Reverse

Name: gatB [H]

Synonym: Bpro_0219

Alternate gene names: 91786130

Gene position: 240287-238785 (Counterclockwise)

Preceding gene: 91786131

Following gene: 91786127

Centisome position: 4.62

GC content: 59.15

Gene sequence:

>1503_bases
ATGAGCAATATCGCAAGCACCCCCACCTCCCCGCTGGTGCAGGGCTACGAAGTCGTCATCGGCTTTGAAACCCACACCCA
GCTGACCACGCAGTCGAAAATCTTCAGCCGCGCCTCCACCGCGTTTGGCGCCGAGCCCAACACGCAGGCCTCGGCCGTCG
ACTTCGCGCTGCCCGGCGCGCTGCCTGTGATGAACAAGGGCGCGGTACAGCGCGCCATCGAGTTTGGCCTGGCCGTCAAT
GCGCACATTGCCGAGCAGAGCGTGTTCGCGCGCAAAAACTACTTCTACCCCGACCTGCCCAAGGGTTACCAGATCAGCCA
GTTCGAGATTCCCGTGGTGCAGGGAGGTGTCGTCGAGTTCTTCCTGGACGGTGAAAAGAAATCGGTGCGACTGGTGCGCG
CCCACCTGGAAGAAGACGCCGGCAAGTCGCTGCACGAAGACTTCATCGGCATGAGCGGCATCGATCTGAACCGCGCCGGC
ACGCCGCTGCTGGAGATCGTGACCGAGCCCGACATGCGCTCGTCGGCCGAAGCCGTGGCTTATGCCAAGGAGTTGCACAA
AATCGTCACCTGGATCGGCATTTGCGACGGCAACATGCAGGAGGGCAGCTTCCGCTGCGATGCCAATGTCTCGGTGCGCA
AGCCCGGCGCAGCGCTGGGCACGCGCCGCGAGATCAAGAACCTGAACAGCTTCAAGTTCATGCAGCAGGCGATCGATTAC
GAAGTGCGCTGGCAGATCGGGCAACTGGAAGACGGCCACGCCATCCAGCAGGCCACCGTGCTGTTTGACCCGGACACCGG
CGAGACACGGGCCATGCGCACCAAGGAAGATGCAGCCGACTACCGCTACTTCCCCGACCCGGATTTGCCGCCGCTGGTGA
TAGCGCGCGACTGGGTGGAGCGCACGCGCGCCGAGATGAGCGAGTTGCCGCGGGTGATGGCGGCGCGCTTCGTCAAGGAC
TATGGCCTGCCCGAGTATGACGCGGCTCAATTGACCTCGTCAAAAGACACTGCCGAGTACTTTGACCGTGCAATGCTTGA
GTTAATTAGCGAAGAGGGGAGCGAGGGAGCTCGCCATGGCAAGACCATTGCCAACTGGATCAATGGGGAGATAGCGGCTC
GAATGAACCGTGCTGGACTCAACAGCATTCGAGAAAGTCCGATCACAGCATGGCACATCGTTACCCTAGTACGTCGAATT
GCCGATGGCACGATCTCAGGAAAAATTGCAAAAGATGAAATTATTCCTGCACTTTGGGATGGAAATTTAGGCTCTCATGA
TGCCGTCAATACGTTCATCGAATCCAAAAAACAAATGAGCGACACCGGCGAGCTGGAAAAAATCATTGATGACGTACTGG
CGGCCAATGCCAAAAACGTCGAAGAGGTCAAGGCCGGCAATGCCAAGGCCTTCAATGCTTTGGTCGGCCAGGCCATGAAA
GCCACCAAGGGCAAGGCCAACCCGGCGCAGGTCAACGAACTGCTGCGGAAAAAACTGGCGTGA

Upstream 100 bases:

>100_bases
ATTATTTCCAGGAAACCCAGCTGCTGGGCGCCGCCCACCATTTCCAGCTGGCCACCGACTGGCACCAAATGAAACCGGAA
GACAAGGCAGGATTGCCGGC

Downstream 100 bases:

>100_bases
TGCCGGCAGGGCCGCCGTGCTGCGGCCCGCCTTCGCGTTCGATACGGAGATTCAGTTTTTGGCGGCGGCCTTGGCTGGCC
CCGCCTTGCCGACGGCGCCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSNIASTPTSPLVQGYEVVIGFETHTQLTTQSKIFSRASTAFGAEPNTQASAVDFALPGALPVMNKGAVQRAIEFGLAVN
AHIAEQSVFARKNYFYPDLPKGYQISQFEIPVVQGGVVEFFLDGEKKSVRLVRAHLEEDAGKSLHEDFIGMSGIDLNRAG
TPLLEIVTEPDMRSSAEAVAYAKELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGAALGTRREIKNLNSFKFMQQAIDY
EVRWQIGQLEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLVIARDWVERTRAEMSELPRVMAARFVKD
YGLPEYDAAQLTSSKDTAEYFDRAMLELISEEGSEGARHGKTIANWINGEIAARMNRAGLNSIRESPITAWHIVTLVRRI
ADGTISGKIAKDEIIPALWDGNLGSHDAVNTFIESKKQMSDTGELEKIIDDVLAANAKNVEEVKAGNAKAFNALVGQAMK
ATKGKANPAQVNELLRKKLA

Sequences:

>Translated_500_residues
MSNIASTPTSPLVQGYEVVIGFETHTQLTTQSKIFSRASTAFGAEPNTQASAVDFALPGALPVMNKGAVQRAIEFGLAVN
AHIAEQSVFARKNYFYPDLPKGYQISQFEIPVVQGGVVEFFLDGEKKSVRLVRAHLEEDAGKSLHEDFIGMSGIDLNRAG
TPLLEIVTEPDMRSSAEAVAYAKELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGAALGTRREIKNLNSFKFMQQAIDY
EVRWQIGQLEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLVIARDWVERTRAEMSELPRVMAARFVKD
YGLPEYDAAQLTSSKDTAEYFDRAMLELISEEGSEGARHGKTIANWINGEIAARMNRAGLNSIRESPITAWHIVTLVRRI
ADGTISGKIAKDEIIPALWDGNLGSHDAVNTFIESKKQMSDTGELEKIIDDVLAANAKNVEEVKAGNAKAFNALVGQAMK
ATKGKANPAQVNELLRKKLA
>Mature_499_residues
SNIASTPTSPLVQGYEVVIGFETHTQLTTQSKIFSRASTAFGAEPNTQASAVDFALPGALPVMNKGAVQRAIEFGLAVNA
HIAEQSVFARKNYFYPDLPKGYQISQFEIPVVQGGVVEFFLDGEKKSVRLVRAHLEEDAGKSLHEDFIGMSGIDLNRAGT
PLLEIVTEPDMRSSAEAVAYAKELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGAALGTRREIKNLNSFKFMQQAIDYE
VRWQIGQLEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLVIARDWVERTRAEMSELPRVMAARFVKDY
GLPEYDAAQLTSSKDTAEYFDRAMLELISEEGSEGARHGKTIANWINGEIAARMNRAGLNSIRESPITAWHIVTLVRRIA
DGTISGKIAKDEIIPALWDGNLGSHDAVNTFIESKKQMSDTGELEKIIDDVLAANAKNVEEVKAGNAKAFNALVGQAMKA
TKGKANPAQVNELLRKKLA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=508, Percent_Identity=37.4015748031496, Blast_Score=329, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI115534094, Length=486, Percent_Identity=30.8641975308642, Blast_Score=218, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=515, Percent_Identity=29.126213592233, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI45550808, Length=501, Percent_Identity=33.3333333333333, Blast_Score=267, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54948; Mature: 54817

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIASTPTSPLVQGYEVVIGFETHTQLTTQSKIFSRASTAFGAEPNTQASAVDFALPGA
CCCCCCCCCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
LPVMNKGAVQRAIEFGLAVNAHIAEQSVFARKNYFYPDLPKGYQISQFEIPVVQGGVVEF
CCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCCCEECEEECCEECCCEEEE
FLDGEKKSVRLVRAHLEEDAGKSLHEDFIGMSGIDLNRAGTPLLEIVTEPDMRSSAEAVA
EECCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHH
YAKELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGAALGTRREIKNLNSFKFMQQAIDY
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEECCCCHHCCHHHHHCCHHHHHHHHHHCE
EVRWQIGQLEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLVIARDWVE
EEEEEECCCCCCCCEEEEEEEECCCCCCCHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHH
RTRAEMSELPRVMAARFVKDYGLPEYDAAQLTSSKDTAEYFDRAMLELISEEGSEGARHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
KTIANWINGEIAARMNRAGLNSIRESPITAWHIVTLVRRIADGTISGKIAKDEIIPALWD
HHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHEEC
GNLGSHDAVNTFIESKKQMSDTGELEKIIDDVLAANAKNVEEVKAGNAKAFNALVGQAMK
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
ATKGKANPAQVNELLRKKLA
HHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SNIASTPTSPLVQGYEVVIGFETHTQLTTQSKIFSRASTAFGAEPNTQASAVDFALPGA
CCCCCCCCCHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
LPVMNKGAVQRAIEFGLAVNAHIAEQSVFARKNYFYPDLPKGYQISQFEIPVVQGGVVEF
CCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCCCCCCCCCEECEEECCEECCCEEEE
FLDGEKKSVRLVRAHLEEDAGKSLHEDFIGMSGIDLNRAGTPLLEIVTEPDMRSSAEAVA
EECCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHH
YAKELHKIVTWIGICDGNMQEGSFRCDANVSVRKPGAALGTRREIKNLNSFKFMQQAIDY
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEECCCCHHCCHHHHHCCHHHHHHHHHHCE
EVRWQIGQLEDGHAIQQATVLFDPDTGETRAMRTKEDAADYRYFPDPDLPPLVIARDWVE
EEEEEECCCCCCCCEEEEEEEECCCCCCCHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHH
RTRAEMSELPRVMAARFVKDYGLPEYDAAQLTSSKDTAEYFDRAMLELISEEGSEGARHG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHH
KTIANWINGEIAARMNRAGLNSIRESPITAWHIVTLVRRIADGTISGKIAKDEIIPALWD
HHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHEEC
GNLGSHDAVNTFIESKKQMSDTGELEKIIDDVLAANAKNVEEVKAGNAKAFNALVGQAMK
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHH
ATKGKANPAQVNELLRKKLA
HHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA