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The map label for this gene is gatA [H]

Identifier: 91786131

GI number: 91786131

Start: 240284

End: 241819

Strand: Reverse

Name: gatA [H]

Synonym: Bpro_0220

Alternate gene names: 91786131

Gene position: 241819-240284 (Counterclockwise)

Preceding gene: 91786132

Following gene: 91786130

Centisome position: 4.65

GC content: 67.25

Gene sequence:

>1536_bases
ATGAGCGCCCCCGACCGCAGCACCGATTTGCACGACCTCGGCGTCGCAGAACTCGCCGCCAGGCTGGCCGCCCGCGAGGT
CTCCAGCGTCGAGGTGACCCGGCATTTCCTTGCCCGCATCCAGCAGCATGAGGGCCTGGGCGCTTTCCTGGCCACCAACC
CGGACGTGTCGCTGGCGCAGGCCCGGGCCGCCGACGCGCGGCTGGCCGCCGGTGAGCGCACGCCTTTGCTGGGCGTGCCG
CTGGCGCACAAGGACGTGTTTGTCACCCGCGACTTCCCGACCACGGCCGGCTCGAAAATGCTGGCCGGCTACCGCAGCCC
GTTCGATGCGACCGTGGTGAGCCGGCTTGGCGTCGCGCCGGGCGGCGCCGGCATGGTCACGCTGGGCAAGCTCAACTGCG
ACGAATTCGCGATGGGCTCGGGCAACGACAACAGCGCCTATCAACCGGTGCACAACCCCTGGGACACCACGCGCGTGCCC
GGCGGCTCCTCGGGCGGCTCGGCGGCGGCTGTGGCGGCACGCTTGGTACCGGCGGCCACCGGCACCGACACCGGCGGCTC
CATCCGCCAGCCCGCCTCGCTCACCGGCATCACCGGCATCAAGCCGACCTATGGCCGCTGCTCGCGCTATGGCATGGTGG
CTTTTGCCTCCAGCCTGGACCAGGCCGGCCCGATGGCGCGCAGCGCACTCGATTGCGCCCTGCTGCTCTCGGCCATGGCC
GGCCCCGACCCCGACCGCGATTCCACCTCGCTGGATCTCCCGGTCACCAATTACGCTGCCGAATTAGCAGCCATAGGCAG
CCAGGAGACACGCTCCATGCCGCTGAAAGGCTTGAGAATCGGCCTGCCCACCCAGTTCTTCGGCGCGGGCTGCTCGGCCG
ATGTGCTCAAGGCGGTTCGCGCCGCGCTGGCCGAATTTGAAAAACTGGGCGCCACATTGGTCGACGTCAGCCTGCCGCTG
ACCGAGCTGTCCATCCCGGTGTACTACGTGATTGCGCCGGCGGAAGCCAGCAGCAACCTGAGCCGCTTTGACGGCGTTCG
CTATGGGCACCGGGCGGCCAGCTACAGCGACCTGACCGACATGTACAAGAAGTCGCGCTCGGAAGGCTTTGGCGACGAGG
TCACGCGCCGCATCATGATTGGCACCTATGTGCTGTCGCACGGCTACTACGATGCCTACTACCTGAAGGCGCAAAAAATC
CGCCGCCTGATTGCGCAGGATTTCCAGAAAGCCTTTGCGCAATGCGACGTGATTGCCGGGCCGGTGTCGCCCACCGTCGC
CTGGAAGATCGGCGAGAAGGAAGACGATCCGGTGGCCAACTACCTGGCCGACATCTACACGCTGTCGTCCAGCCTGGCTG
GCCTGCCCGGCATGAGCATCCCGGCGGGCCTGGGCGCCCACGGCATGCCGGTGGGCCTGCAGCTGATCGGCAATTATTTC
CAGGAAACCCAGCTGCTGGGCGCCGCCCACCATTTCCAGCTGGCCACCGACTGGCACCAAATGAAACCGGAAGACAAGGC
AGGATTGCCGGCATGA

Upstream 100 bases:

>100_bases
TGCGCGATGACATCGCCAGCGAGCCCAACCAGCGCGAGGCCAGCCAGGTCAGCGCCCCCGCCGTGGAACGCGGCCTGTTT
CTCGTGCCCAAGGTGATCGA

Downstream 100 bases:

>100_bases
GCAATATCGCAAGCACCCCCACCTCCCCGCTGGTGCAGGGCTACGAAGTCGTCATCGGCTTTGAAACCCACACCCAGCTG
ACCACGCAGTCGAAAATCTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSAPDRSTDLHDLGVAELAARLAAREVSSVEVTRHFLARIQQHEGLGAFLATNPDVSLAQARAADARLAAGERTPLLGVP
LAHKDVFVTRDFPTTAGSKMLAGYRSPFDATVVSRLGVAPGGAGMVTLGKLNCDEFAMGSGNDNSAYQPVHNPWDTTRVP
GGSSGGSAAAVAARLVPAATGTDTGGSIRQPASLTGITGIKPTYGRCSRYGMVAFASSLDQAGPMARSALDCALLLSAMA
GPDPDRDSTSLDLPVTNYAAELAAIGSQETRSMPLKGLRIGLPTQFFGAGCSADVLKAVRAALAEFEKLGATLVDVSLPL
TELSIPVYYVIAPAEASSNLSRFDGVRYGHRAASYSDLTDMYKKSRSEGFGDEVTRRIMIGTYVLSHGYYDAYYLKAQKI
RRLIAQDFQKAFAQCDVIAGPVSPTVAWKIGEKEDDPVANYLADIYTLSSSLAGLPGMSIPAGLGAHGMPVGLQLIGNYF
QETQLLGAAHHFQLATDWHQMKPEDKAGLPA

Sequences:

>Translated_511_residues
MSAPDRSTDLHDLGVAELAARLAAREVSSVEVTRHFLARIQQHEGLGAFLATNPDVSLAQARAADARLAAGERTPLLGVP
LAHKDVFVTRDFPTTAGSKMLAGYRSPFDATVVSRLGVAPGGAGMVTLGKLNCDEFAMGSGNDNSAYQPVHNPWDTTRVP
GGSSGGSAAAVAARLVPAATGTDTGGSIRQPASLTGITGIKPTYGRCSRYGMVAFASSLDQAGPMARSALDCALLLSAMA
GPDPDRDSTSLDLPVTNYAAELAAIGSQETRSMPLKGLRIGLPTQFFGAGCSADVLKAVRAALAEFEKLGATLVDVSLPL
TELSIPVYYVIAPAEASSNLSRFDGVRYGHRAASYSDLTDMYKKSRSEGFGDEVTRRIMIGTYVLSHGYYDAYYLKAQKI
RRLIAQDFQKAFAQCDVIAGPVSPTVAWKIGEKEDDPVANYLADIYTLSSSLAGLPGMSIPAGLGAHGMPVGLQLIGNYF
QETQLLGAAHHFQLATDWHQMKPEDKAGLPA
>Mature_510_residues
SAPDRSTDLHDLGVAELAARLAAREVSSVEVTRHFLARIQQHEGLGAFLATNPDVSLAQARAADARLAAGERTPLLGVPL
AHKDVFVTRDFPTTAGSKMLAGYRSPFDATVVSRLGVAPGGAGMVTLGKLNCDEFAMGSGNDNSAYQPVHNPWDTTRVPG
GSSGGSAAAVAARLVPAATGTDTGGSIRQPASLTGITGIKPTYGRCSRYGMVAFASSLDQAGPMARSALDCALLLSAMAG
PDPDRDSTSLDLPVTNYAAELAAIGSQETRSMPLKGLRIGLPTQFFGAGCSADVLKAVRAALAEFEKLGATLVDVSLPLT
ELSIPVYYVIAPAEASSNLSRFDGVRYGHRAASYSDLTDMYKKSRSEGFGDEVTRRIMIGTYVLSHGYYDAYYLKAQKIR
RLIAQDFQKAFAQCDVIAGPVSPTVAWKIGEKEDDPVANYLADIYTLSSSLAGLPGMSIPAGLGAHGMPVGLQLIGNYFQ
ETQLLGAAHHFQLATDWHQMKPEDKAGLPA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=36.7265469061876, Blast_Score=305, Evalue=8e-83,
Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=26.5182186234818, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17543272, Length=459, Percent_Identity=37.037037037037, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=249, Percent_Identity=30.1204819277108, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=257, Percent_Identity=31.1284046692607, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=202, Percent_Identity=31.1881188118812, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=134, Percent_Identity=32.8358208955224, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=440, Percent_Identity=35.4545454545455, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=430, Percent_Identity=29.7674418604651, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24648113, Length=506, Percent_Identity=38.5375494071146, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24652985, Length=512, Percent_Identity=26.3671875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922090, Length=512, Percent_Identity=26.3671875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652981, Length=512, Percent_Identity=26.3671875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24652983, Length=512, Percent_Identity=26.3671875, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=24.9512670565302, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161078093, Length=538, Percent_Identity=26.3940520446097, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648435, Length=268, Percent_Identity=30.5970149253731, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648437, Length=268, Percent_Identity=30.5970149253731, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550774, Length=268, Percent_Identity=30.5970149253731, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356731, Length=240, Percent_Identity=32.0833333333333, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648441, Length=191, Percent_Identity=33.5078534031414, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648439, Length=191, Percent_Identity=33.5078534031414, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53843; Mature: 53711

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPDRSTDLHDLGVAELAARLAAREVSSVEVTRHFLARIQQHEGLGAFLATNPDVSLAQ
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ARAADARLAAGERTPLLGVPLAHKDVFVTRDFPTTAGSKMLAGYRSPFDATVVSRLGVAP
HHHHHHHHCCCCCCCEEECCCCCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHCCCC
GGAGMVTLGKLNCDEFAMGSGNDNSAYQPVHNPWDTTRVPGGSSGGSAAAVAARLVPAAT
CCCCEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
GTDTGGSIRQPASLTGITGIKPTYGRCSRYGMVAFASSLDQAGPMARSALDCALLLSAMA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GPDPDRDSTSLDLPVTNYAAELAAIGSQETRSMPLKGLRIGLPTQFFGAGCSADVLKAVR
CCCCCCCCCEECCCHHHHHHHHHHCCCCHHHCCCCCCEEECCCHHHHCCCCCHHHHHHHH
AALAEFEKLGATLVDVSLPLTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAASYSDLTD
HHHHHHHHHCCEEEEEECCHHHHCCCEEEEEECCCCCCCHHHHCCCCCCCHHCCHHHHHH
MYKKSRSEGFGDEVTRRIMIGTYVLSHGYYDAYYLKAQKIRRLIAQDFQKAFAQCDVIAG
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PVSPTVAWKIGEKEDDPVANYLADIYTLSSSLAGLPGMSIPAGLGAHGMPVGLQLIGNYF
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QETQLLGAAHHFQLATDWHQMKPEDKAGLPA
HHHHHHHHHHHHEECCCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SAPDRSTDLHDLGVAELAARLAAREVSSVEVTRHFLARIQQHEGLGAFLATNPDVSLAQ
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHH
ARAADARLAAGERTPLLGVPLAHKDVFVTRDFPTTAGSKMLAGYRSPFDATVVSRLGVAP
HHHHHHHHCCCCCCCEEECCCCCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHCCCC
GGAGMVTLGKLNCDEFAMGSGNDNSAYQPVHNPWDTTRVPGGSSGGSAAAVAARLVPAAT
CCCCEEEEECCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
GTDTGGSIRQPASLTGITGIKPTYGRCSRYGMVAFASSLDQAGPMARSALDCALLLSAMA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GPDPDRDSTSLDLPVTNYAAELAAIGSQETRSMPLKGLRIGLPTQFFGAGCSADVLKAVR
CCCCCCCCCEECCCHHHHHHHHHHCCCCHHHCCCCCCEEECCCHHHHCCCCCHHHHHHHH
AALAEFEKLGATLVDVSLPLTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAASYSDLTD
HHHHHHHHHCCEEEEEECCHHHHCCCEEEEEECCCCCCCHHHHCCCCCCCHHCCHHHHHH
MYKKSRSEGFGDEVTRRIMIGTYVLSHGYYDAYYLKAQKIRRLIAQDFQKAFAQCDVIAG
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PVSPTVAWKIGEKEDDPVANYLADIYTLSSSLAGLPGMSIPAGLGAHGMPVGLQLIGNYF
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QETQLLGAAHHFQLATDWHQMKPEDKAGLPA
HHHHHHHHHHHHEECCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA