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The map label for this gene is amtB [H]

Identifier: 91786095

GI number: 91786095

Start: 199113

End: 200660

Strand: Direct

Name: amtB [H]

Synonym: Bpro_0184

Alternate gene names: 91786095

Gene position: 199113-200660 (Clockwise)

Preceding gene: 91786094

Following gene: 91786096

Centisome position: 3.83

GC content: 62.86

Gene sequence:

>1548_bases
ATGAAAAAATTTCTTGCTTCCCTTGTATTGGGATTGAGTCTGCTGGGAACTGGCGCAGCCGTCATGGCGCAGGCGCCCGC
TGCAGCGCCTGCCGCGTCCGCCGCCGAAGCCAAGCCGGCTGAGGCAGCTGCACCAGCGGCACCGGCCGCCGCAGCAGCCC
CGGCGGCCGCAGCTGCAGCGCCCGCGGCTTCCGCCGCAGCAGCTCCGGCCCCCGTCCCCAACAAGGGAGACACCGCCTGG
ATGATGGTTTCCACCCTGCTGGTGATCATGATGACCGTGCCGGGTCTGGCCCTGTTCTATGGCGGCCTGGTCCGCAGCAA
GAACATGCTATCGGTCCTGATGCAGGTCATGGTGACGTTCTCCATGATTGTGGTGCTGTGGTTCATCTATGGCTACAGCC
TGGCATTCACGGAGGGCAACGCATTCTTTGGCGGCTTTGACCGGCTGTTCATGAAAGGAGTCTGGGACAACGCGGCCGGC
ACTTTCGCCAACGCCGCGACCTTCAGCAAGGGCGTCGTGATTCCTGAAATCGTGTTTGCCGCCTTCCAGGCAACGTTCGC
CGGCATCACCTGCACCCTGATCGTTGGCGCCTTTGCCGAACGCATCAAGTTCTCGGCCGTGCTGCTCTTCATGGCCATCT
GGTTCACCTTCAGCTACGCGCCGATTGCGCACATGGTGTGGTTCTGGATGGGGCCTGATGCCTACACGGGCAAGGAAGTG
GTTGACGCGATGACCGGCAAGGCCGGATACATCTGGCAAATGGGCGCGCTGGACTTTGCCGGCGGTACCGTCGTGCACAT
CAACGCGGCTGTCGCCGGCCTGGTGGGTGCCTTTGTGATTGGCAAGCGTATCGGCTACGGCAAGGAATCGATGGCGCCTC
ACAGCCTCACGCTGACCATGGTCGGATCCGCCTTGCTGTGGGTCGGCTGGTTTGGTTTCAACGCCGGCTCGGCGCTGGAA
GCCAATGGTTTTGCCGCCCTGGCATTCATCAACACCCTCGGTGCCACCGCCGCCGCTGTGCTGGCCTGGTGTGTCGGTGA
ATCGCTGATGCGTGGCAAGGCTTCGATGCTGGGTGCTGCATCCGGTGCGGTTGCGGGTCTGGTGGCGATCACGCCAGCAG
CCGGCAATGTGGGTATCGGTGGTGGTCTGGTGATCGGTCTGGCGGCTGGCTTTGCCTGCCTCTGGGGCGTGAATGGCCTG
AAGAAACTGCTGGGTGCCGACGACTCGCTCGACGTGTTCGGCGTGCACGGCATCGGCGGTATTCTGGGCGCCTTGCTCAC
CGGCGTGTTCAATTCCCCGAGCCTGGGCGGCCCCGGCTATGTGGCCGACTGGACGACGGCCACCATGGTGACCGCTGCCG
ATTTCTCGGTCGTGTCGCAACTGTGGATCCAGGCCAAGGCCGTGCTGATCACCATCGTCTGGTCTGGCGTGGTTTCCTTC
ATCGCCTACAAGATTGTTGACCTGACGATCGGCCTGCGTGTGAGCGAAGAAGACGAGCGCGAAGGTCTGGACATCACCGC
ACACGGTGAAACGGCCTACAACCGCTAA

Upstream 100 bases:

>100_bases
TGTCTATAACCTCGAGCAGGTTGTGCGCATCCGCACCGGTGAAACCGGCAAGGAAGCGCTCTGACGCGCGGTTCAACCCT
AGAAAAAAGAGAACATCGAC

Downstream 100 bases:

>100_bases
GAGGAATAGGAATGCCGGGACTGCCCTGAGGGCAGTCCCTTGATCAACGAGAGTCTCCTTTGGATGTTCAGCCCGCCGGT
GCCAAGCCGGTGGGCTTTTT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MKKFLASLVLGLSLLGTGAAVMAQAPAAAPAASAAEAKPAEAAAPAAPAAAAAPAAAAAAPAASAAAAPAPVPNKGDTAW
MMVSTLLVIMMTVPGLALFYGGLVRSKNMLSVLMQVMVTFSMIVVLWFIYGYSLAFTEGNAFFGGFDRLFMKGVWDNAAG
TFANAATFSKGVVIPEIVFAAFQATFAGITCTLIVGAFAERIKFSAVLLFMAIWFTFSYAPIAHMVWFWMGPDAYTGKEV
VDAMTGKAGYIWQMGALDFAGGTVVHINAAVAGLVGAFVIGKRIGYGKESMAPHSLTLTMVGSALLWVGWFGFNAGSALE
ANGFAALAFINTLGATAAAVLAWCVGESLMRGKASMLGAASGAVAGLVAITPAAGNVGIGGGLVIGLAAGFACLWGVNGL
KKLLGADDSLDVFGVHGIGGILGALLTGVFNSPSLGGPGYVADWTTATMVTAADFSVVSQLWIQAKAVLITIVWSGVVSF
IAYKIVDLTIGLRVSEEDEREGLDITAHGETAYNR

Sequences:

>Translated_515_residues
MKKFLASLVLGLSLLGTGAAVMAQAPAAAPAASAAEAKPAEAAAPAAPAAAAAPAAAAAAPAASAAAAPAPVPNKGDTAW
MMVSTLLVIMMTVPGLALFYGGLVRSKNMLSVLMQVMVTFSMIVVLWFIYGYSLAFTEGNAFFGGFDRLFMKGVWDNAAG
TFANAATFSKGVVIPEIVFAAFQATFAGITCTLIVGAFAERIKFSAVLLFMAIWFTFSYAPIAHMVWFWMGPDAYTGKEV
VDAMTGKAGYIWQMGALDFAGGTVVHINAAVAGLVGAFVIGKRIGYGKESMAPHSLTLTMVGSALLWVGWFGFNAGSALE
ANGFAALAFINTLGATAAAVLAWCVGESLMRGKASMLGAASGAVAGLVAITPAAGNVGIGGGLVIGLAAGFACLWGVNGL
KKLLGADDSLDVFGVHGIGGILGALLTGVFNSPSLGGPGYVADWTTATMVTAADFSVVSQLWIQAKAVLITIVWSGVVSF
IAYKIVDLTIGLRVSEEDEREGLDITAHGETAYNR
>Mature_515_residues
MKKFLASLVLGLSLLGTGAAVMAQAPAAAPAASAAEAKPAEAAAPAAPAAAAAPAAAAAAPAASAAAAPAPVPNKGDTAW
MMVSTLLVIMMTVPGLALFYGGLVRSKNMLSVLMQVMVTFSMIVVLWFIYGYSLAFTEGNAFFGGFDRLFMKGVWDNAAG
TFANAATFSKGVVIPEIVFAAFQATFAGITCTLIVGAFAERIKFSAVLLFMAIWFTFSYAPIAHMVWFWMGPDAYTGKEV
VDAMTGKAGYIWQMGALDFAGGTVVHINAAVAGLVGAFVIGKRIGYGKESMAPHSLTLTMVGSALLWVGWFGFNAGSALE
ANGFAALAFINTLGATAAAVLAWCVGESLMRGKASMLGAASGAVAGLVAITPAAGNVGIGGGLVIGLAAGFACLWGVNGL
KKLLGADDSLDVFGVHGIGGILGALLTGVFNSPSLGGPGYVADWTTATMVTAADFSVVSQLWIQAKAVLITIVWSGVVSF
IAYKIVDLTIGLRVSEEDEREGLDITAHGETAYNR

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=449, Percent_Identity=54.7884187082405, Blast_Score=465, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17550182, Length=466, Percent_Identity=27.6824034334764, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17550180, Length=456, Percent_Identity=27.8508771929825, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=454, Percent_Identity=24.4493392070485, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI133931100, Length=461, Percent_Identity=24.0780911062907, Blast_Score=89, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=458, Percent_Identity=32.7510917030568, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6325396, Length=452, Percent_Identity=31.4159292035398, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321559, Length=445, Percent_Identity=29.438202247191, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI161078314, Length=455, Percent_Identity=25.7142857142857, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52591; Mature: 52591

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHEEEEECCCHHHCCCEEEECCCCCCCC
>Mature Secondary Structure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HHHHHHHHHEEEEECCCHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]