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The map label for this gene is iciA [H]

Identifier: 91786060

GI number: 91786060

Start: 155593

End: 156507

Strand: Direct

Name: iciA [H]

Synonym: Bpro_0149

Alternate gene names: 91786060

Gene position: 155593-156507 (Clockwise)

Preceding gene: 91786058

Following gene: 91786061

Centisome position: 2.99

GC content: 66.56

Gene sequence:

>915_bases
ATGAGTACGTTTGACCCCGATGCGCTGGAGTGCCTGGCGGCGATTGTGGAAGAGGGTGGTTTTGAACGCGCAGCCCAGCG
CCTGTCGATCACGCAGTCGGCGGTGTCGCAGCGTCTGCGCGCGCTGGAGGCGCAGGTCGGCACCGTGCTGATCGTGCGCA
GCCGGCCGCTCAAGCCGACCTCCGCGGGCCAGCTGCTGCTCAAGCACACCAAGATGCTGCGTCTGCTGCGCGCCGACCTG
GAGCGCGACCTCAAGGAGCTGGCGCCGAGCTGGCTGGGCGGCGCGCGCGAGGAAGAGCGCATCTCGATCGCCATCAACGC
CGACAGCATTGCCACCTGGGCCATGCAGGCGCTGACCGACCTGGCGCAGCAGGGCCTGCCCATGGAAATCATCGCGGACG
ACCAGGACTTCACCCAGGAATGGCTGCGCGAAGGCCAGGTGCTGGGTTGCGTCACCACACTCAGGCAGGCCCTGCGCGGC
TGCAAGGTGGTCTCTCTGGGCGCCATGGAATATGTCGCCGTGGCCGCACCGGAATTTGCGCGGGACCGGCTGGCCCATGC
GACGCTGACCGCCCACAATTTTCGCGACATCCCCTTCGTGGCGTTCAACCGCAAGGACGACATGCAGAGCGAGTTTGTCG
GCAAGGCCTTCGGGCTCAAGCGTGTCACGCTCAATCAGCTGTTTGTGCCGTCGTCGGAAGGCCAGGTGCGTGCGGTGCTG
GCCGGTTGGGGCGCCAGCGTGGTACCGCGGCTGCTGGCGCAAGGGCTGATTGATCAGGGTCTGCTGGTCAATGTGCTGCC
GACCTGCACACTGCCGATCCAGCTGTACTGGCATTGCTGGAATCTGGAGTCCGAGGTGCTGGATGCGCTCACCGCGGCAC
TGCGGCAGGCGGCGGCCCTGTCGCTGGTGCCTTGA

Upstream 100 bases:

>100_bases
CCTTTCCGTGCATTCGAGTGTGCAGGATGTTGCTTTCAATTAAAAGAAAAATTAAACTCTTAGTGAAAACATCAGAACAG
CTAATACTGAGGAAGCTCCC

Downstream 100 bases:

>100_bases
CGCGCCGGGCGACAGCCGCCAGCCTGCATGCAACCGAGAGCCGTTCCCCGCTGCCGGCCCGGAGGCTGACGGCTCGCTGA
CGCCTGTCCCTTTTTTAGGG

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSTFDPDALECLAAIVEEGGFERAAQRLSITQSAVSQRLRALEAQVGTVLIVRSRPLKPTSAGQLLLKHTKMLRLLRADL
ERDLKELAPSWLGGAREEERISIAINADSIATWAMQALTDLAQQGLPMEIIADDQDFTQEWLREGQVLGCVTTLRQALRG
CKVVSLGAMEYVAVAAPEFARDRLAHATLTAHNFRDIPFVAFNRKDDMQSEFVGKAFGLKRVTLNQLFVPSSEGQVRAVL
AGWGASVVPRLLAQGLIDQGLLVNVLPTCTLPIQLYWHCWNLESEVLDALTAALRQAAALSLVP

Sequences:

>Translated_304_residues
MSTFDPDALECLAAIVEEGGFERAAQRLSITQSAVSQRLRALEAQVGTVLIVRSRPLKPTSAGQLLLKHTKMLRLLRADL
ERDLKELAPSWLGGAREEERISIAINADSIATWAMQALTDLAQQGLPMEIIADDQDFTQEWLREGQVLGCVTTLRQALRG
CKVVSLGAMEYVAVAAPEFARDRLAHATLTAHNFRDIPFVAFNRKDDMQSEFVGKAFGLKRVTLNQLFVPSSEGQVRAVL
AGWGASVVPRLLAQGLIDQGLLVNVLPTCTLPIQLYWHCWNLESEVLDALTAALRQAAALSLVP
>Mature_303_residues
STFDPDALECLAAIVEEGGFERAAQRLSITQSAVSQRLRALEAQVGTVLIVRSRPLKPTSAGQLLLKHTKMLRLLRADLE
RDLKELAPSWLGGAREEERISIAINADSIATWAMQALTDLAQQGLPMEIIADDQDFTQEWLREGQVLGCVTTLRQALRGC
KVVSLGAMEYVAVAAPEFARDRLAHATLTAHNFRDIPFVAFNRKDDMQSEFVGKAFGLKRVTLNQLFVPSSEGQVRAVLA
GWGASVVPRLLAQGLIDQGLLVNVLPTCTLPIQLYWHCWNLESEVLDALTAALRQAAALSLVP

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=298, Percent_Identity=36.5771812080537, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786713, Length=297, Percent_Identity=26.5993265993266, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33322; Mature: 33191

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFDPDALECLAAIVEEGGFERAAQRLSITQSAVSQRLRALEAQVGTVLIVRSRPLKPT
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCC
SAGQLLLKHTKMLRLLRADLERDLKELAPSWLGGAREEERISIAINADSIATWAMQALTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHH
LAQQGLPMEIIADDQDFTQEWLREGQVLGCVTTLRQALRGCKVVSLGAMEYVAVAAPEFA
HHHCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCCCHHH
RDRLAHATLTAHNFRDIPFVAFNRKDDMQSEFVGKAFGLKRVTLNQLFVPSSEGQVRAVL
HHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHEECCCCCCCEEEEE
AGWGASVVPRLLAQGLIDQGLLVNVLPTCTLPIQLYWHCWNLESEVLDALTAALRQAAAL
ECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
SLVP
CCCC
>Mature Secondary Structure 
STFDPDALECLAAIVEEGGFERAAQRLSITQSAVSQRLRALEAQVGTVLIVRSRPLKPT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCC
SAGQLLLKHTKMLRLLRADLERDLKELAPSWLGGAREEERISIAINADSIATWAMQALTD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHH
LAQQGLPMEIIADDQDFTQEWLREGQVLGCVTTLRQALRGCKVVSLGAMEYVAVAAPEFA
HHHCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHCCCHHH
RDRLAHATLTAHNFRDIPFVAFNRKDDMQSEFVGKAFGLKRVTLNQLFVPSSEGQVRAVL
HHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHEECCCCCCCEEEEE
AGWGASVVPRLLAQGLIDQGLLVNVLPTCTLPIQLYWHCWNLESEVLDALTAALRQAAAL
ECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHH
SLVP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA