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The map label for this gene is nemA [H]

Identifier: 91785976

GI number: 91785976

Start: 66905

End: 68005

Strand: Reverse

Name: nemA [H]

Synonym: Bpro_0063

Alternate gene names: 91785976

Gene position: 68005-66905 (Counterclockwise)

Preceding gene: 91785982

Following gene: 91785967

Centisome position: 1.31

GC content: 68.39

Gene sequence:

>1101_bases
ATGCCTTCTTTATTCGACCCCACCCAGGCCGGCGACATTCATCTGGCCAACCGCATCGTGCTGGCGCCCCTGACACGCAA
CCGCGCCCCCGACGCCATTCCCACCTCGCTGATGGCCGAGTACTATGCCCAGCGCGCCAGCGGCGGCCTGCTCGTCACCG
AAGCCACCGCCATCAGCCACCAGGGCCAGGGCTATGCCGATGTGCCCGGGCTCTATGGCACTGCGCAGCTCGACGGCTGG
AAAGAGGTGACCCGCGCCGTGCATGAGGCCGGCGGGAAGATCGTGGTGCAGCTCTGGCATGTGGGCCGCGTCTCGCACAC
CGAGTTGCAGCCGGACGGCGGCCAGCCGGTCGCGCCGTCCGCCATCACCGCCAGGACCAAGACCGTGCTGCTCAAGGACG
GCGCGCCCACCTTTGTCGCCACCTCCGAGCCGCGCGCACTGCGGGCCGACGAACTGCCCGGCATCGTGCATGCCTATGAA
GCCGCGGCGCGCAATGCGGTCGAAACCGCTGGCTTTGACGGCGTCGAAGTCCACGGCGCCAACGGCTACCTGCTCGACCA
GTTCCTCAAGCGGGGCTCCAACCAGCGCACCGACGACTACGGCGGCAGCATCGAGAACCGCGCGCGCCTGCTGCTCGAGG
TCACGCGCGCCGTCACCGGCGCGATCGGCGGCGGACGCACCGGCATTCGCCTGTCGCCGGTGACACCGGCCAACGATGTG
CATGACGCCGATCCGCAGCCGCTGTTCGACTACGTGGTGCGCCAGCTCGCCACGCTGAACCTGGCCTATATCCACATCAT
CGAAGGCGCGACCGGCGGGCCGCGCGAAATCGCCGACCGGCCGTTCGACTATGCCGCGCTCAAGACCGCCTACCGCGAAG
CCGGCGGCAAGGGCGCCTGGATGGTCAACAACGGCTACGACCAGGCACTGGCCAAGCAGGCGGTGGCGAACGGCGCCGAC
CTGATCGCCTTTGGCAAGCCCTACATCGCCAACCCGGATCTGGTGGAGCGCCTGCGCCGCAATGCGCCGCTCAACCTGCC
CGACCAGGCCACCTTTTATGGCGGCGGCGCCAAGGGTTATACGGACTACCCAACGCTATAA

Upstream 100 bases:

>100_bases
ACGCCAATTCAGATCGGCACACACTGACGCCTGCTCACTCTTCCTGTTTCCTGTTTCCTGTTTCATCTTCCCGATTCATC
TTTTCAACCCGGACACTTTC

Downstream 100 bases:

>100_bases
AACCAGACCGCATGCCAGTCATGCATGACTGGCAATGGTTTCAGGGCTTTCTTATTTAAACGAGGCCCTGGCACAGGTAT
TTGGTGTGCATGTAGTCTTC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MPSLFDPTQAGDIHLANRIVLAPLTRNRAPDAIPTSLMAEYYAQRASGGLLVTEATAISHQGQGYADVPGLYGTAQLDGW
KEVTRAVHEAGGKIVVQLWHVGRVSHTELQPDGGQPVAPSAITARTKTVLLKDGAPTFVATSEPRALRADELPGIVHAYE
AAARNAVETAGFDGVEVHGANGYLLDQFLKRGSNQRTDDYGGSIENRARLLLEVTRAVTGAIGGGRTGIRLSPVTPANDV
HDADPQPLFDYVVRQLATLNLAYIHIIEGATGGPREIADRPFDYAALKTAYREAGGKGAWMVNNGYDQALAKQAVANGAD
LIAFGKPYIANPDLVERLRRNAPLNLPDQATFYGGGAKGYTDYPTL

Sequences:

>Translated_366_residues
MPSLFDPTQAGDIHLANRIVLAPLTRNRAPDAIPTSLMAEYYAQRASGGLLVTEATAISHQGQGYADVPGLYGTAQLDGW
KEVTRAVHEAGGKIVVQLWHVGRVSHTELQPDGGQPVAPSAITARTKTVLLKDGAPTFVATSEPRALRADELPGIVHAYE
AAARNAVETAGFDGVEVHGANGYLLDQFLKRGSNQRTDDYGGSIENRARLLLEVTRAVTGAIGGGRTGIRLSPVTPANDV
HDADPQPLFDYVVRQLATLNLAYIHIIEGATGGPREIADRPFDYAALKTAYREAGGKGAWMVNNGYDQALAKQAVANGAD
LIAFGKPYIANPDLVERLRRNAPLNLPDQATFYGGGAKGYTDYPTL
>Mature_365_residues
PSLFDPTQAGDIHLANRIVLAPLTRNRAPDAIPTSLMAEYYAQRASGGLLVTEATAISHQGQGYADVPGLYGTAQLDGWK
EVTRAVHEAGGKIVVQLWHVGRVSHTELQPDGGQPVAPSAITARTKTVLLKDGAPTFVATSEPRALRADELPGIVHAYEA
AARNAVETAGFDGVEVHGANGYLLDQFLKRGSNQRTDDYGGSIENRARLLLEVTRAVTGAIGGGRTGIRLSPVTPANDVH
DADPQPLFDYVVRQLATLNLAYIHIIEGATGGPREIADRPFDYAALKTAYREAGGKGAWMVNNGYDQALAKQAVANGADL
IAFGKPYIANPDLVERLRRNAPLNLPDQATFYGGGAKGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=48.5094850948509, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1789463, Length=226, Percent_Identity=34.9557522123894, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17559802, Length=354, Percent_Identity=27.9661016949153, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=269, Percent_Identity=27.1375464684015, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=267, Percent_Identity=28.0898876404494, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=298, Percent_Identity=28.1879194630872, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=136, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17564188, Length=274, Percent_Identity=26.2773722627737, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17566914, Length=234, Percent_Identity=26.4957264957265, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=385, Percent_Identity=37.9220779220779, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321973, Length=381, Percent_Identity=37.7952755905512, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39002; Mature: 38871

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLFDPTQAGDIHLANRIVLAPLTRNRAPDAIPTSLMAEYYAQRASGGLLVTEATAISH
CCCCCCCCCCCCEEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHC
QGQGYADVPGLYGTAQLDGWKEVTRAVHEAGGKIVVQLWHVGRVSHTELQPDGGQPVAPS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEECCCCCCCCCCC
AITARTKTVLLKDGAPTFVATSEPRALRADELPGIVHAYEAAARNAVETAGFDGVEVHGA
HHEECEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
NGYLLDQFLKRGSNQRTDDYGGSIENRARLLLEVTRAVTGAIGGGRTGIRLSPVTPANDV
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
HDADPQPLFDYVVRQLATLNLAYIHIIEGATGGPREIADRPFDYAALKTAYREAGGKGAW
CCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEE
MVNNGYDQALAKQAVANGADLIAFGKPYIANPDLVERLRRNAPLNLPDQATFYGGGAKGY
EEECCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCC
TDYPTL
CCCCCC
>Mature Secondary Structure 
PSLFDPTQAGDIHLANRIVLAPLTRNRAPDAIPTSLMAEYYAQRASGGLLVTEATAISH
CCCCCCCCCCCEEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHC
QGQGYADVPGLYGTAQLDGWKEVTRAVHEAGGKIVVQLWHVGRVSHTELQPDGGQPVAPS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEECCCCCCCCCCC
AITARTKTVLLKDGAPTFVATSEPRALRADELPGIVHAYEAAARNAVETAGFDGVEVHGA
HHEECEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECC
NGYLLDQFLKRGSNQRTDDYGGSIENRARLLLEVTRAVTGAIGGGRTGIRLSPVTPANDV
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCC
HDADPQPLFDYVVRQLATLNLAYIHIIEGATGGPREIADRPFDYAALKTAYREAGGKGAW
CCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHCCCCEEE
MVNNGYDQALAKQAVANGADLIAFGKPYIANPDLVERLRRNAPLNLPDQATFYGGGAKGY
EEECCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCC
TDYPTL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]