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The map label for this gene is thiE [H]

Identifier: 91785954

GI number: 91785954

Start: 44115

End: 44762

Strand: Direct

Name: thiE [H]

Synonym: Bpro_0041

Alternate gene names: 91785954

Gene position: 44115-44762 (Clockwise)

Preceding gene: 91785953

Following gene: 91785955

Centisome position: 0.85

GC content: 69.91

Gene sequence:

>648_bases
ATGAATACAGACGCTTTTGCTCCATTGACCGGCCCCATCGGCTTTTACCCCGTGGTGCCCGATGCCGCCTGGGTGCAGCG
TCTGCTGGGCTGGGGTGTGCGTACCATCCAGCTGCGCATCAAGGCGGCGGACCACACGCCGGCCGAGATCGAGCGCGAAG
TGCGCGCGGCGGTCGAGGCCGGGCGCGCCGTGCCGGGCGCGCAGGTCTTCATCAACGACCACTGGCAGCTGGCGCTGGCC
GCCGGCGCCTATGGCGTGCACCTGGGCCAGGAAGACCTGAGCATGGCGGAGGCGGACCTCAATGCCCTGCGCAAGGCGGG
TGTGCGGCTGGGCCTGAGCACGCACACGCCGGCCGAGCTGGCGCGTGCCCATGCGGTGCAGCCCTCCTACCTGGCGATCG
GTCCGGTCTACCCGACCCTGCTCAAGGTCATGCCCTACGAGCCGGTGGGCCTGGAGCGCCTCAAGGAATGGGCGCTGCGG
GCCGTACCGTATTCCGTGGTGGCGATAGGCGGCATTTCGCTGGCACGCTTGCCTGGCGTGATAGCCTGCGGCGTGGATGG
CGTGGCGGTGGTCAGCGCGGTGACGCAGGCGGCTGACCCCGAGCAGGCCACCAGGCAGGCACTCGCCCTCTTTGACGCGC
CCGCTTGA

Upstream 100 bases:

>100_bases
CGGAATCCTCATGCACGCAAGTGCATTCCGGTGTCCTGCGCTCCGTGCGCCTTGCACTGCACTCCGATAATCGAATTTCT
GACCGCCCCTTGAGACCACC

Downstream 100 bases:

>100_bases
TGTGAATCAAGGTGCGCCCGCCTGAGTGGTGGGATGCTGGGTGCCACTGAGTTTGTTCTGGGGAAAGACGATCATGGCCT
ACGCAACCCTGCGCATCATT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNTDAFAPLTGPIGFYPVVPDAAWVQRLLGWGVRTIQLRIKAADHTPAEIEREVRAAVEAGRAVPGAQVFINDHWQLALA
AGAYGVHLGQEDLSMAEADLNALRKAGVRLGLSTHTPAELARAHAVQPSYLAIGPVYPTLLKVMPYEPVGLERLKEWALR
AVPYSVVAIGGISLARLPGVIACGVDGVAVVSAVTQAADPEQATRQALALFDAPA

Sequences:

>Translated_215_residues
MNTDAFAPLTGPIGFYPVVPDAAWVQRLLGWGVRTIQLRIKAADHTPAEIEREVRAAVEAGRAVPGAQVFINDHWQLALA
AGAYGVHLGQEDLSMAEADLNALRKAGVRLGLSTHTPAELARAHAVQPSYLAIGPVYPTLLKVMPYEPVGLERLKEWALR
AVPYSVVAIGGISLARLPGVIACGVDGVAVVSAVTQAADPEQATRQALALFDAPA
>Mature_215_residues
MNTDAFAPLTGPIGFYPVVPDAAWVQRLLGWGVRTIQLRIKAADHTPAEIEREVRAAVEAGRAVPGAQVFINDHWQLALA
AGAYGVHLGQEDLSMAEADLNALRKAGVRLGLSTHTPAELARAHAVQPSYLAIGPVYPTLLKVMPYEPVGLERLKEWALR
AVPYSVVAIGGISLARLPGVIACGVDGVAVVSAVTQAADPEQATRQALALFDAPA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=54.1463414634146, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22694; Mature: 22694

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDAFAPLTGPIGFYPVVPDAAWVQRLLGWGVRTIQLRIKAADHTPAEIEREVRAAVEA
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHHH
GRAVPGAQVFINDHWQLALAAGAYGVHLGQEDLSMAEADLNALRKAGVRLGLSTHTPAEL
CCCCCCCEEEECCCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCEECCCCCCHHHH
ARAHAVQPSYLAIGPVYPTLLKVMPYEPVGLERLKEWALRAVPYSVVAIGGISLARLPGV
HHHHCCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHEEEECCCHHHHCCCE
IACGVDGVAVVSAVTQAADPEQATRQALALFDAPA
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTDAFAPLTGPIGFYPVVPDAAWVQRLLGWGVRTIQLRIKAADHTPAEIEREVRAAVEA
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHHH
GRAVPGAQVFINDHWQLALAAGAYGVHLGQEDLSMAEADLNALRKAGVRLGLSTHTPAEL
CCCCCCCEEEECCCEEEEEECCCCEEECCHHHHHHHHHHHHHHHHHCCEECCCCCCHHHH
ARAHAVQPSYLAIGPVYPTLLKVMPYEPVGLERLKEWALRAVPYSVVAIGGISLARLPGV
HHHHCCCCCEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHEEEECCCHHHHCCCE
IACGVDGVAVVSAVTQAADPEQATRQALALFDAPA
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]