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The map label for this gene is dnaN [H]

Identifier: 91785915

GI number: 91785915

Start: 1723

End: 2829

Strand: Direct

Name: dnaN [H]

Synonym: Bpro_0002

Alternate gene names: 91785915

Gene position: 1723-2829 (Clockwise)

Preceding gene: 91785914

Following gene: 91785916

Centisome position: 0.03

GC content: 60.34

Gene sequence:

>1107_bases
ATGATCGTTCTGAAAGCCACCCAGGATAAAGTTTTGTCGGTACTGCAATCGGTCGCGGGCATCGTGGAGCGCAGGCACAC
CTTGCCGATCCTGGCCAACGTGCTGATCCGCAAAACCGGGGCGCAGCTGCAGCTCACCACGAGCGATCTTGAAATCCAGA
TCCGCACCAGCGCCGACCTTGATGGCGATGCCGGCAACTTCACCACCACGGTGGGTGCGCGCAAGCTCATCGACATCCTG
CGGTCCATGCCCAGCGACCAGACCGTGTCGCTCGAGTCGGCGCAAAGCAAGCTGATCCTCAAAGGCGGCAAGAGCCGCTT
CACGCTGCAAACCCTGCCCGCCGAAGACTTCCCGCTGGTGCAGGAAGCCGCCAACTTCGGTCCCAGCTTCTCGGTACCGC
AAAAGGTGCTCAAGGAGCTGCTCAACCAGGTGTCGTTTGCGATGGCGGTGCACGACATCCGCTACTACCTCAACGGCATT
TTGTTTGTGGCTGAAGGCAAGCAGCTGAGCCTGGTGGCCACCGACGGCCACCGCCTGGCGTTCTCGTCGGCCACGCTCGA
TGTGGAAGTGCCCAAGCAGGAAGTCATCCTGCCGCGCAAGACGGTGCTGGAAATGCAGCGCCTGCTCAGCGACAAGGAAG
GCGCCATCGAGATGCAGTTTGCCGGTAACCAGGCCAAGTTCAGTTTTGAGGGCATGGAGTTTGTCACCAAGCTGGTCGAA
GGCAAATTTCCCGACTACAACCGCGTGATTCCGAAGAACCACAAAAACATCATCACGCTGGGCCGCGTGCCGCTGCTGGC
CAGCCTGCAGCGCACCGCCATTCTCACCAGCGAGAAGTTCAAGGGCGTGCGCCTGAACATCGAACCCGGTACGCTGCGGG
TGGCGTCCAACAACGCCGAGCAGGAAGAGGCCGTTGACGAGCTCGACATCGACTACGGCGGCGATGCCATCGAAATCGGC
TTCAACGTGACCTACCTGATCGACGCGCTGGCCAACATGGAGCAGGACATGGTGAAGATCGAGCTGGCCGACTCCAACAG
CTCGGCGCTGCTCACCATTCCCGACGATGCGGCCTTTAAATACGTGGTGATGCCCATGCGCATTTGA

Upstream 100 bases:

>100_bases
GATCAAAAAGGGGAAAATAGCGCTATCCACAGATTTGCGTGGCCCTTACTACTACTACTAATTTGAATTAAAGAATTAAA
GATAAATACAAGAGGAACTC

Downstream 100 bases:

>100_bases
CGTATTTGGTCACGCTAAAAAGCGCGCCCAAATTCAAACAGACCCGCTTGCTAGAGCGGGTTTGGTCATTCAAGAGATTG
AAGAAATCGAAAAGATTTGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIVLKATQDKVLSVLQSVAGIVERRHTLPILANVLIRKTGAQLQLTTSDLEIQIRTSADLDGDAGNFTTTVGARKLIDIL
RSMPSDQTVSLESAQSKLILKGGKSRFTLQTLPAEDFPLVQEAANFGPSFSVPQKVLKELLNQVSFAMAVHDIRYYLNGI
LFVAEGKQLSLVATDGHRLAFSSATLDVEVPKQEVILPRKTVLEMQRLLSDKEGAIEMQFAGNQAKFSFEGMEFVTKLVE
GKFPDYNRVIPKNHKNIITLGRVPLLASLQRTAILTSEKFKGVRLNIEPGTLRVASNNAEQEEAVDELDIDYGGDAIEIG
FNVTYLIDALANMEQDMVKIELADSNSSALLTIPDDAAFKYVVMPMRI

Sequences:

>Translated_368_residues
MIVLKATQDKVLSVLQSVAGIVERRHTLPILANVLIRKTGAQLQLTTSDLEIQIRTSADLDGDAGNFTTTVGARKLIDIL
RSMPSDQTVSLESAQSKLILKGGKSRFTLQTLPAEDFPLVQEAANFGPSFSVPQKVLKELLNQVSFAMAVHDIRYYLNGI
LFVAEGKQLSLVATDGHRLAFSSATLDVEVPKQEVILPRKTVLEMQRLLSDKEGAIEMQFAGNQAKFSFEGMEFVTKLVE
GKFPDYNRVIPKNHKNIITLGRVPLLASLQRTAILTSEKFKGVRLNIEPGTLRVASNNAEQEEAVDELDIDYGGDAIEIG
FNVTYLIDALANMEQDMVKIELADSNSSALLTIPDDAAFKYVVMPMRI
>Mature_368_residues
MIVLKATQDKVLSVLQSVAGIVERRHTLPILANVLIRKTGAQLQLTTSDLEIQIRTSADLDGDAGNFTTTVGARKLIDIL
RSMPSDQTVSLESAQSKLILKGGKSRFTLQTLPAEDFPLVQEAANFGPSFSVPQKVLKELLNQVSFAMAVHDIRYYLNGI
LFVAEGKQLSLVATDGHRLAFSSATLDVEVPKQEVILPRKTVLEMQRLLSDKEGAIEMQFAGNQAKFSFEGMEFVTKLVE
GKFPDYNRVIPKNHKNIITLGRVPLLASLQRTAILTSEKFKGVRLNIEPGTLRVASNNAEQEEAVDELDIDYGGDAIEIG
FNVTYLIDALANMEQDMVKIELADSNSSALLTIPDDAAFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=361, Percent_Identity=40.1662049861496, Blast_Score=277, Evalue=7e-76,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40506; Mature: 40506

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLKATQDKVLSVLQSVAGIVERRHTLPILANVLIRKTGAQLQLTTSDLEIQIRTSADL
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCC
DGDAGNFTTTVGARKLIDILRSMPSDQTVSLESAQSKLILKGGKSRFTLQTLPAEDFPLV
CCCCCCEEEHHHHHHHHHHHHCCCCCCEEEHHHHCCEEEEECCCCEEEEEECCCCCCCHH
QEAANFGPSFSVPQKVLKELLNQVSFAMAVHDIRYYLNGILFVAEGKQLSLVATDGHRLA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEEECCCEEE
FSSATLDVEVPKQEVILPRKTVLEMQRLLSDKEGAIEMQFAGNQAKFSFEGMEFVTKLVE
EECCEEEEECCHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHHHHHC
GKFPDYNRVIPKNHKNIITLGRVPLLASLQRTAILTSEKFKGVRLNIEPGTLRVASNNAE
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHEEECCCCCCEEEEECCCEEEEECCCCH
QEEAVDELDIDYGGDAIEIGFNVTYLIDALANMEQDMVKIELADSNSSALLTIPDDAAFK
HHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC
>Mature Secondary Structure
MIVLKATQDKVLSVLQSVAGIVERRHTLPILANVLIRKTGAQLQLTTSDLEIQIRTSADL
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCC
DGDAGNFTTTVGARKLIDILRSMPSDQTVSLESAQSKLILKGGKSRFTLQTLPAEDFPLV
CCCCCCEEEHHHHHHHHHHHHCCCCCCEEEHHHHCCEEEEECCCCEEEEEECCCCCCCHH
QEAANFGPSFSVPQKVLKELLNQVSFAMAVHDIRYYLNGILFVAEGKQLSLVATDGHRLA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEEECCCEEE
FSSATLDVEVPKQEVILPRKTVLEMQRLLSDKEGAIEMQFAGNQAKFSFEGMEFVTKLVE
EECCEEEEECCHHHCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHHHHHC
GKFPDYNRVIPKNHKNIITLGRVPLLASLQRTAILTSEKFKGVRLNIEPGTLRVASNNAE
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHEEECCCCCCEEEEECCCEEEEECCCCH
QEEAVDELDIDYGGDAIEIGFNVTYLIDALANMEQDMVKIELADSNSSALLTIPDDAAFK
HHHHHHHCCCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]