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The map label for this gene is leuB [H]

Identifier: 229558816

GI number: 229558816

Start: 3811394

End: 3812479

Strand: Direct

Name: leuB [H]

Synonym: Bpro_3610

Alternate gene names: 229558816

Gene position: 3811394-3812479 (Clockwise)

Preceding gene: 91789460

Following gene: 91789463

Centisome position: 73.29

GC content: 63.72

Gene sequence:

>1086_bases
ATGAAAATCGCAATTCTCCCGGGTGACGGCATCGGCACCGAAATCGTCGCGGAAGCGGTCAAGGTCCTGAACGTTCTGGA
CCTGAAGTTTGAAACCGAAACCGCGCTGGTCGGCGGCGCCGCCTACGAGGCCCACGGCCACCCCCTGCCCGAGGCGACGC
TGAAGCTCGCCAAGGACGCCGACGCCGTGCTGTTTGGCGCGGTCGGTGACTGGAAATACGACAAGCTGGACCGTCCGCTG
CGTCCCGAGCAAGCCATTTTGGGCCTGCGCAAAAACCTGGGCCTGTTTGCCAACTTCCGCCCGGCCATTTGCTACCCCCA
ACTGGTGGAGGCTTCCAGCCTCAAGCGCGAGCTGGTGGCGGGCCTGGACATCCTCATCATCCGCGAACTGACCGGCGACA
TCTACTTCGGCCAGCCGCGCGGCCGCCGCGTGGCCACCGACGGGCACTTTCCCGGTGCAGAGGAAGCCTTTGACACCATG
CGCTACTCGCGCCCCGAGATCGAGCGCATCGCCCATGTGGCTTTCCAGGCGGCGCGCAAGCGCAAGGCTTCGGGGGGCGA
GGGACGCGTCACCAGCGTCGACAAGGCCAATGTGCTGGAGACCTTCCAGTTCTGGAAAGACGTTGTGACGGAAATCGGGC
TGCAGTATCCCGACGTGGCACTGGACCACATGTACGTGGACAACGCCGCCATGCAACTGGTCAGGGCACCCAAGAAATTT
GACGTGGTGGTCACGGGCAACATGTTCGGCGACATCCTGTCGGATGCCGCTGCCATGCTGACCGGCAGCATCGGCATGCT
GCCTTCGGCCAGCCTGAACGACAAAAACCAGGGCCTGTACGAACCCAGCCACGGCAGTGCGCCCGACATCGCGGGCAAAG
GCGTGGCCAACCCCCTGGCCACCATTTTGAGCGCCGCCATGATGCTGCGCTTCAGCCTGAACCAGCCAGAAACCGCCGCG
CGCATTGAAAAAGCCGTGGATGCGGTGCTCAGCCAGGGGCTGCGCACCCCTGACATCTACAGCGAAGGCACGACCAAGGT
AGGCACCGTGCAGATGGGCGATGCTGTCGTGAAGGCGCTGAGATAG

Upstream 100 bases:

>100_bases
AACCACACCATCCCGGGTTGATAAGCCGGTATTTTTCAGTCAAATCATGCTTTTGCCCCATCAATATGGGCATGAGCAGC
CATACAAAAAGATAGCAACC

Downstream 100 bases:

>100_bases
TTGGCCCCCACGGTCGCTCACTGCGTGTAGCTCCCTGCCCCCCGAGGGGGCCGCTGCGCCTGCGGTCTGGCAAAGCCAGT
CCCGCGGCCCCGGCTGATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKIAILPGDGIGTEIVAEAVKVLNVLDLKFETETALVGGAAYEAHGHPLPEATLKLAKDADAVLFGAVGDWKYDKLDRPL
RPEQAILGLRKNLGLFANFRPAICYPQLVEASSLKRELVAGLDILIIRELTGDIYFGQPRGRRVATDGHFPGAEEAFDTM
RYSRPEIERIAHVAFQAARKRKASGGEGRVTSVDKANVLETFQFWKDVVTEIGLQYPDVALDHMYVDNAAMQLVRAPKKF
DVVVTGNMFGDILSDAAAMLTGSIGMLPSASLNDKNQGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRFSLNQPETAA
RIEKAVDAVLSQGLRTPDIYSEGTTKVGTVQMGDAVVKALR

Sequences:

>Translated_361_residues
MKIAILPGDGIGTEIVAEAVKVLNVLDLKFETETALVGGAAYEAHGHPLPEATLKLAKDADAVLFGAVGDWKYDKLDRPL
RPEQAILGLRKNLGLFANFRPAICYPQLVEASSLKRELVAGLDILIIRELTGDIYFGQPRGRRVATDGHFPGAEEAFDTM
RYSRPEIERIAHVAFQAARKRKASGGEGRVTSVDKANVLETFQFWKDVVTEIGLQYPDVALDHMYVDNAAMQLVRAPKKF
DVVVTGNMFGDILSDAAAMLTGSIGMLPSASLNDKNQGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRFSLNQPETAA
RIEKAVDAVLSQGLRTPDIYSEGTTKVGTVQMGDAVVKALR
>Mature_361_residues
MKIAILPGDGIGTEIVAEAVKVLNVLDLKFETETALVGGAAYEAHGHPLPEATLKLAKDADAVLFGAVGDWKYDKLDRPL
RPEQAILGLRKNLGLFANFRPAICYPQLVEASSLKRELVAGLDILIIRELTGDIYFGQPRGRRVATDGHFPGAEEAFDTM
RYSRPEIERIAHVAFQAARKRKASGGEGRVTSVDKANVLETFQFWKDVVTEIGLQYPDVALDHMYVDNAAMQLVRAPKKF
DVVVTGNMFGDILSDAAAMLTGSIGMLPSASLNDKNQGLYEPSHGSAPDIAGKGVANPLATILSAAMMLRFSLNQPETAA
RIEKAVDAVLSQGLRTPDIYSEGTTKVGTVQMGDAVVKALR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=342, Percent_Identity=31.8713450292398, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4758582, Length=345, Percent_Identity=28.695652173913, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=27.4760383386581, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=27.4760383386581, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI28178838, Length=338, Percent_Identity=27.810650887574, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI28178819, Length=149, Percent_Identity=34.8993288590604, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=51.6574585635359, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1788101, Length=373, Percent_Identity=33.5120643431635, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=25.0620347394541, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=31.7919075144509, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=365, Percent_Identity=28.7671232876712, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=339, Percent_Identity=30.0884955752212, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=312, Percent_Identity=30.7692307692308, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=374, Percent_Identity=43.8502673796791, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=36.7688022284123, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324291, Length=339, Percent_Identity=31.5634218289086, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324709, Length=366, Percent_Identity=31.1475409836066, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=31.8965517241379, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=31.8965517241379, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24661184, Length=379, Percent_Identity=28.4960422163588, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078635, Length=256, Percent_Identity=27.734375, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078637, Length=256, Percent_Identity=27.734375, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078633, Length=256, Percent_Identity=27.734375, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24650122, Length=256, Percent_Identity=27.734375, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI161078639, Length=256, Percent_Identity=27.734375, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281362242, Length=356, Percent_Identity=28.0898876404494, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648872, Length=356, Percent_Identity=28.0898876404494, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130355, Length=174, Percent_Identity=29.8850574712644, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38903; Mature: 38903

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAILPGDGIGTEIVAEAVKVLNVLDLKFETETALVGGAAYEAHGHPLPEATLKLAKDA
CEEEEECCCCCCHHHHHHHHHHHHHHHCEECCCHHEECCCEECCCCCCCCHHHHHHHCCC
DAVLFGAVGDWKYDKLDRPLRPEQAILGLRKNLGLFANFRPAICYPQLVEASSLKRELVA
CEEEEECCCCCCHHHCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
GLDILIIRELTGDIYFGQPRGRRVATDGHFPGAEEAFDTMRYSRPEIERIAHVAFQAARK
CCHHEEEEECCCCEEECCCCCCEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RKASGGEGRVTSVDKANVLETFQFWKDVVTEIGLQYPDVALDHMYVDNAAMQLVRAPKKF
HCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCE
DVVVTGNMFGDILSDAAAMLTGSIGMLPSASLNDKNQGLYEPSHGSAPDIAGKGVANPLA
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
TILSAAMMLRFSLNQPETAARIEKAVDAVLSQGLRTPDIYSEGTTKVGTVQMGDAVVKAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHC
R
C
>Mature Secondary Structure
MKIAILPGDGIGTEIVAEAVKVLNVLDLKFETETALVGGAAYEAHGHPLPEATLKLAKDA
CEEEEECCCCCCHHHHHHHHHHHHHHHCEECCCHHEECCCEECCCCCCCCHHHHHHHCCC
DAVLFGAVGDWKYDKLDRPLRPEQAILGLRKNLGLFANFRPAICYPQLVEASSLKRELVA
CEEEEECCCCCCHHHCCCCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHH
GLDILIIRELTGDIYFGQPRGRRVATDGHFPGAEEAFDTMRYSRPEIERIAHVAFQAARK
CCHHEEEEECCCCEEECCCCCCEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RKASGGEGRVTSVDKANVLETFQFWKDVVTEIGLQYPDVALDHMYVDNAAMQLVRAPKKF
HCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCE
DVVVTGNMFGDILSDAAAMLTGSIGMLPSASLNDKNQGLYEPSHGSAPDIAGKGVANPLA
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
TILSAAMMLRFSLNQPETAARIEKAVDAVLSQGLRTPDIYSEGTTKVGTVQMGDAVVKAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA