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The map label for this gene is cniT [H]

Identifier: 91213976

GI number: 91213976

Start: 4928263

End: 4929804

Strand: Direct

Name: cniT [H]

Synonym: UTI89_C5028

Alternate gene names: 91213976

Gene position: 4928263-4929804 (Clockwise)

Preceding gene: 91213975

Following gene: 91213981

Centisome position: 97.29

GC content: 44.36

Gene sequence:

>1542_bases
ATGCACTATTTAAAAAAACGCTTTTCTTTGATTGAACTGAATGTGTTTATCCCAGCAATATTATTTATTGCGGTAATCAT
TCTGTGTTTGACGATATATCCACAAGATACCAGCCGGTATATAAATAAAATACATCATTTTTTAACCTGGGAAATGGGCG
GGATTTTTCTGGTCATGACTTTTCTGGTTGTACTTTGTTGTCTGTGGCTGGCCTTCTCACGTTATGGCGATATCTTGTTA
GGTCAGTCGGGAGAAAAACCTGACTTCAGCTTGTTAACCTGGCTTGGACTTATTTTTACTTCTGGAACGGGGGGCAGCTT
GTTATATCTGGCCTCTGTAGAGTGGATTTGGATCATTCAGCAACCGCCTTTTGGCGCAACAGCAGGAAGCGCTCAGGCTG
CTCGTTGGGCCTCTGTTTATGGCATGTTCCATTGGGGGCCGTCCGCATGGGCATGGTATTTGATTTGCGCCGTTCCTATT
GGTTGGTTTATGCATGTTAAGAAAACAAACTCATTAAAGGTCAGTGATTTATGTCGTGGATGTTTGGGAGCGCGTGCGGA
TGGTTTTTGCGGGCATTGTGTGAATTTTTTCTACATGTTTGGTTTGCTCGGCGGCGCGGTAACGTCTCTGGCGCTGGGAA
CGCCGATGATTTCTGCCGTATTTTGCCATGTGTTCCATCTGGATCCTGCCGGGCAGTTTATCAATGTCATGGTTATTTTT
ATCTGGACGCTAGTACCATTATTCATTCTCTTTTTTGGACTTAAAAAAGGTGTGGCATGGGCCAGTAACTGGAATATTCG
TGCTGACATTTTGATGCTACTGGCAATACTGATTTGTGGACCAACGGCATTTATACTTAATCAATCAATTGATGGTCTCG
GCCTGATGCTGCAAAATTTTGTAGCGATGAGTTTAAGTACCGATGCTATTGGTCGTAGCGGATTTCCACAGATGTGGACC
GTATTTTATTTTTCATGGTGGGTCGTGTATGCCATCCCATTCGGTTTATTTATCGCCCGTATTTCAAAAGGAAGGACGAT
CCGGCAATTGATTGTATGTGGAACTCTGGCAGGTTCATTGGGATGCATGGTTTTTTACATGGTACTGGCTAATTTCGGCT
TATCGTTGCAGACAACTCATGTCATCGATTTTGTTCCCATACTTAACGAACAAGGGCGAGGCGTTGTCGTTTCTCGTTTA
CTGGAGCAGCTACCCGCAAGTCAGGTGTTTTTGGTTGCTTTTGGGGCTATAGCATTAATTTCATATATTACCGGACACTG
TACTGTGGGTTATGCCCTCGGTTTTGCGACGCAAAAACGGGCAGATAGTGAGAGTGAACCGGCATTCTGGAACGTGGCAT
TTTGGTTGATTATGACTGGAATCGTCGCCATCACACTCTATCTTCTTGATGCGCAAAGTCTGCAACCGCTACAAACGGTG
TCTATTCTGGCTGGACTACCGCTTTGCGGCGTGGTGTTTATTTTATTGAAGAGTTTTTTGACGCAGCTTGCGGCTGAAGA
GAAAAACGCGAGAGATGAATAA

Upstream 100 bases:

>100_bases
GCGCCTTTTTTTCTTTACGCCTTTCCCGACAATTGCAACACATGAAAAAGGAACGGCGAAATATTCGACCTCATTGAATG
AAAACATTCAGGAGACTGAA

Downstream 100 bases:

>100_bases
TGATGTTCAGGAATGCCCGGTAATTTGCATTATCGGGCATTTTACCGAACGGGAATTAACTCAGGATCGCACGAAAATAT
CCCGCCAGATTTTCTCCAAG

Product: putative carnitine transporter CniT

Products: Proton [Cytoplasm]; L-carnitine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MHYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKIHHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILL
GQSGEKPDFSLLTWLGLIFTSGTGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASVYGMFHWGPSAWAWYLICAVPI
GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMISAVFCHVFHLDPAGQFINVMVIF
IWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAILICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWT
VFYFSWWVVYAIPFGLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQGRGVVVSRL
LEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFWNVAFWLIMTGIVAITLYLLDAQSLQPLQTV
SILAGLPLCGVVFILLKSFLTQLAAEEKNARDE

Sequences:

>Translated_513_residues
MHYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKIHHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILL
GQSGEKPDFSLLTWLGLIFTSGTGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASVYGMFHWGPSAWAWYLICAVPI
GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMISAVFCHVFHLDPAGQFINVMVIF
IWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAILICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWT
VFYFSWWVVYAIPFGLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQGRGVVVSRL
LEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFWNVAFWLIMTGIVAITLYLLDAQSLQPLQTV
SILAGLPLCGVVFILLKSFLTQLAAEEKNARDE
>Mature_513_residues
MHYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKIHHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILL
GQSGEKPDFSLLTWLGLIFTSGTGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASVYGMFHWGPSAWAWYLICAVPI
GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMISAVFCHVFHLDPAGQFINVMVIF
IWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAILICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWT
VFYFSWWVVYAIPFGLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQGRGVVVSRL
LEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFWNVAFWLIMTGIVAITLYLLDAQSLQPLQTV
SILAGLPLCGVVFILLKSFLTQLAAEEKNARDE

Specific function: Catalyzes the exchange of L-carnitine for gamma- butyrobetaine and related betaines [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family. CaiT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786224, Length=511, Percent_Identity=30.9197651663405, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1788102, Length=451, Percent_Identity=28.3813747228381, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1786506, Length=499, Percent_Identity=26.25250501002, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56765; Mature: 56765

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKIHHFLTWEMGGIFLVMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
FLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSGTGGSLLYLASVEWIWIIQ
HHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEE
QPPFGATAGSAQAARWASVYGMFHWGPSAWAWYLICAVPIGWFMHVKKTNSLKVSDLCRG
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCEEHHHHHHH
CLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMISAVFCHVFHLDPAGQFINVMVIF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAILICGPTAFILNQSIDGLGLMLQNF
HHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHH
VAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPFGLFIARISKGRTIRQLIVCGTLAGSL
HHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GCMVFYMVLANFGLSLQTTHVIDFVPILNEQGRGVVVSRLLEQLPASQVFLVAFGAIALI
HHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
SYITGHCTVGYALGFATQKRADSESEPAFWNVAFWLIMTGIVAITLYLLDAQSLQPLQTV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SILAGLPLCGVVFILLKSFLTQLAAEEKNARDE
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MHYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKIHHFLTWEMGGIFLVMT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHH
FLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSGTGGSLLYLASVEWIWIIQ
HHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEE
QPPFGATAGSAQAARWASVYGMFHWGPSAWAWYLICAVPIGWFMHVKKTNSLKVSDLCRG
CCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEECCCCEEHHHHHHH
CLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMISAVFCHVFHLDPAGQFINVMVIF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHH
IWTLVPLFILFFGLKKGVAWASNWNIRADILMLLAILICGPTAFILNQSIDGLGLMLQNF
HHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHH
VAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPFGLFIARISKGRTIRQLIVCGTLAGSL
HHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GCMVFYMVLANFGLSLQTTHVIDFVPILNEQGRGVVVSRLLEQLPASQVFLVAFGAIALI
HHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
SYITGHCTVGYALGFATQKRADSESEPAFWNVAFWLIMTGIVAITLYLLDAQSLQPLQTV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SILAGLPLCGVVFILLKSFLTQLAAEEKNARDE
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-carnitine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-carnitine [Periplasm] = Proton [Cytoplasm] + L-carnitine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA