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The map label for this gene is gyrA1 [H]

Identifier: 89898786

GI number: 89898786

Start: 1130501

End: 1131940

Strand: Direct

Name: gyrA1 [H]

Synonym: CF0979

Alternate gene names: 89898786

Gene position: 1130501-1131940 (Clockwise)

Preceding gene: 89898785

Following gene: 89898787

Centisome position: 96.94

GC content: 39.38

Gene sequence:

>1440_bases
ATGCAGGACGTTTCAGATCTTTTTAAGACGCATTTTATGCACTACGCATCTTATGTGATTTTAGAAAGAGCCATTCCCCA
CATTCTTGATGGTCTTAAACCCGTACAACGACGTTTGCTATGGACCTTGTTTCGCATGGACGATGGCAAAATGCACAAGG
TTGCCAATATTGCTGGTAGAACTATGGCCCTGCATCCCCATGGGGATGCTCCCATTGTTGAGGCTCTCGTTGTCCTAGCA
AATAAAGGTTACCTGATTGACACTCAGGGAAACTTCGGGAATCCCCTAACCGGGGATCCCCATGCTGCAGCAAGATATAT
TGAAGCGCGCCTTAGTCCCCTAGCTAAAGACATTCTATTTAACACAGATTTAATGTCTTTCCATGATTCCTACGATGGCA
GAGAAAAAGAGCCTGACATTCTTCCTGCAAAGCTCCCCTTACTTTTGCTGCATGGTGTTGAAGGAATTGCTGTAGGAATG
ACAACGAAAATTTTCCCGCACAATCTCTATGAACTTATTGAGGCACAAATTGCCATTTTAAATAACCGTACATTCACCCT
ACTTCCCGATTTTTATTCTGGAGGTGTGATGGATGCCTCAGAATATCAAGATGGTTTAGGTTCTATAACAATTCGCGCCT
CTATTCAAATAGTAGATTCAAAGACCTTAATCATAAAAGAAATCTGTCCTTCAACAACAACCGAGACTTTGATTCGCTCT
ATCGAAAACGCAGCAAAACGCGGTGTAATCAAAATTGATTCGATTCAAGATTTCTCAACAGATCAACCTCATATAGAGAT
AAAGCTCCCCAAAGGAATCTATGCTAAGGATATCCTAGAACCTCTATTCCAATACACAGAATGCCAAGTTGTTTTAACGT
CTAAACCGACGGCGATTTATAATAATAAGCCCGTCGAAACATCCATTTCTGAAATTTTGAAGCTTCACACGGAAGCTCTT
GAAGGGTACTTACAAAAAGAACTACAGATTCTTCATGATGAGCTGGCTCAAGAACATTATTATAAATCTTTAGAATACAT
TTTTATTAAGCACCGCTTATACGACATAGTCAGAGAGCTCCTCTCTAAGTTAGGAAATAAAGTCTCTCAAGAAGACCTTC
ATGAAGCAGTATTAGCCTCTTTAGCTCCTTTCATCTCGAGTTTACCTGAGGCTCCTTGCAAACAAGCCACGAGTCAACTT
GCTTCATTAGCGATAAAAAAAATCCTCTGCTTTAACGAAACTAGCTATACGAAAGACCTGGAAGGGATAAAGAAAAAACA
AACTGCTGTGAAAAAAGATCTGAGCAATATGAAGAAGTTTACGATTAAATATCTCAAGGGTCTTTTAGCAAAATATGGCG
AGCTTGGAAAAAGAAAAACACAAATTTTATCGTTTTCTAACCAAAAGAAATCTGTTCTTAAACAACAAACTTTAGTGTGA

Upstream 100 bases:

>100_bases
ATTAGAATCCGTAGAGACTCTTTTACAATTTTATATGGGGAAAAATACCAAAGAACGAAAACAATTTATTATGGACAACC
TCATTACCAACTTATAGCTT

Downstream 100 bases:

>100_bases
TTAGAAACAAAAATTTTTTATTTTTTAAAAACTTTTTATTTCATGATTATAGAAAATAACTTCCCAGCATAGTAAAATGC
GTAATTAAGCTAGGCATCGA

Product: DNA topoisomerase IV subunit A

Products: NA

Alternate protein names: Topoisomerase IV subunit A [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MQDVSDLFKTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFRMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLAKDILFNTDLMSFHDSYDGREKEPDILPAKLPLLLLHGVEGIAVGM
TTKIFPHNLYELIEAQIAILNNRTFTLLPDFYSGGVMDASEYQDGLGSITIRASIQIVDSKTLIIKEICPSTTTETLIRS
IENAAKRGVIKIDSIQDFSTDQPHIEIKLPKGIYAKDILEPLFQYTECQVVLTSKPTAIYNNKPVETSISEILKLHTEAL
EGYLQKELQILHDELAQEHYYKSLEYIFIKHRLYDIVRELLSKLGNKVSQEDLHEAVLASLAPFISSLPEAPCKQATSQL
ASLAIKKILCFNETSYTKDLEGIKKKQTAVKKDLSNMKKFTIKYLKGLLAKYGELGKRKTQILSFSNQKKSVLKQQTLV

Sequences:

>Translated_479_residues
MQDVSDLFKTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFRMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLAKDILFNTDLMSFHDSYDGREKEPDILPAKLPLLLLHGVEGIAVGM
TTKIFPHNLYELIEAQIAILNNRTFTLLPDFYSGGVMDASEYQDGLGSITIRASIQIVDSKTLIIKEICPSTTTETLIRS
IENAAKRGVIKIDSIQDFSTDQPHIEIKLPKGIYAKDILEPLFQYTECQVVLTSKPTAIYNNKPVETSISEILKLHTEAL
EGYLQKELQILHDELAQEHYYKSLEYIFIKHRLYDIVRELLSKLGNKVSQEDLHEAVLASLAPFISSLPEAPCKQATSQL
ASLAIKKILCFNETSYTKDLEGIKKKQTAVKKDLSNMKKFTIKYLKGLLAKYGELGKRKTQILSFSNQKKSVLKQQTLV
>Mature_479_residues
MQDVSDLFKTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFRMDDGKMHKVANIAGRTMALHPHGDAPIVEALVVLA
NKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLAKDILFNTDLMSFHDSYDGREKEPDILPAKLPLLLLHGVEGIAVGM
TTKIFPHNLYELIEAQIAILNNRTFTLLPDFYSGGVMDASEYQDGLGSITIRASIQIVDSKTLIIKEICPSTTTETLIRS
IENAAKRGVIKIDSIQDFSTDQPHIEIKLPKGIYAKDILEPLFQYTECQVVLTSKPTAIYNNKPVETSISEILKLHTEAL
EGYLQKELQILHDELAQEHYYKSLEYIFIKHRLYDIVRELLSKLGNKVSQEDLHEAVLASLAPFISSLPEAPCKQATSQL
ASLAIKKILCFNETSYTKDLEGIKKKQTAVKKDLSNMKKFTIKYLKGLLAKYGELGKRKTQILSFSNQKKSVLKQQTLV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=260, Percent_Identity=29.2307692307692, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19913406, Length=228, Percent_Identity=29.8245614035088, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788562, Length=341, Percent_Identity=32.5513196480938, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789396, Length=254, Percent_Identity=35.4330708661417, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI212645845, Length=169, Percent_Identity=34.9112426035503, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535065, Length=163, Percent_Identity=32.5153374233129, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115534553, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=183, Percent_Identity=32.2404371584699, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136538, Length=245, Percent_Identity=29.7959183673469, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760 [H]

Pfam domain/function: PF00521 DNA_topoisoIV [H]

EC number: 5.99.1.3

Molecular weight: Translated: 53903; Mature: 53903

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDVSDLFKTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFRMDDGKMHKVANIAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
TMALHPHGDAPIVEALVVLANKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLAKDILF
EEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NTDLMSFHDSYDGREKEPDILPAKLPLLLLHGVEGIAVGMTTKIFPHNLYELIEAQIAIL
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHCHHHHHHHHHHHHHHC
NNRTFTLLPDFYSGGVMDASEYQDGLGSITIRASIQIVDSKTLIIKEICPSTTTETLIRS
CCCEEEECCHHHCCCCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHCCCCHHHHHHHH
IENAAKRGVIKIDSIQDFSTDQPHIEIKLPKGIYAKDILEPLFQYTECQVVLTSKPTAIY
HHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCEEE
NNKPVETSISEILKLHTEALEGYLQKELQILHDELAQEHYYKSLEYIFIKHRLYDIVREL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKLGNKVSQEDLHEAVLASLAPFISSLPEAPCKQATSQLASLAIKKILCFNETSYTKDL
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EGIKKKQTAVKKDLSNMKKFTIKYLKGLLAKYGELGKRKTQILSFSNQKKSVLKQQTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQDVSDLFKTHFMHYASYVILERAIPHILDGLKPVQRRLLWTLFRMDDGKMHKVANIAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
TMALHPHGDAPIVEALVVLANKGYLIDTQGNFGNPLTGDPHAAARYIEARLSPLAKDILF
EEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NTDLMSFHDSYDGREKEPDILPAKLPLLLLHGVEGIAVGMTTKIFPHNLYELIEAQIAIL
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHCHHHHHHHHHHHHHHC
NNRTFTLLPDFYSGGVMDASEYQDGLGSITIRASIQIVDSKTLIIKEICPSTTTETLIRS
CCCEEEECCHHHCCCCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHCCCCHHHHHHHH
IENAAKRGVIKIDSIQDFSTDQPHIEIKLPKGIYAKDILEPLFQYTECQVVLTSKPTAIY
HHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCEEE
NNKPVETSISEILKLHTEALEGYLQKELQILHDELAQEHYYKSLEYIFIKHRLYDIVREL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKLGNKVSQEDLHEAVLASLAPFISSLPEAPCKQATSQLASLAIKKILCFNETSYTKDL
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EGIKKKQTAVKKDLSNMKKFTIKYLKGLLAKYGELGKRKTQILSFSNQKKSVLKQQTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]