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The map label for this gene is gyrB1 [H]

Identifier: 89898785

GI number: 89898785

Start: 1128689

End: 1130497

Strand: Direct

Name: gyrB1 [H]

Synonym: CF0978

Alternate gene names: 89898785

Gene position: 1128689-1130497 (Clockwise)

Preceding gene: 89898771

Following gene: 89898786

Centisome position: 96.78

GC content: 37.2

Gene sequence:

>1809_bases
ATGGCAACATATACTGAAGCTAGTGTTGTTTCTCTAGCCTCCCTAGAGCATATTCGCCTACGCGCGGGTATGTATATAGG
CAGATTAGGAGACGGTTCCCAAATTGAAGATGGGATTTACACTTTATTCAAAGAAGTTGTGGACAACGCAATAGATGAAT
TCATCATGGGTCATGGGAAGACTATTTCCATCTCTACCAATGCCACCACGATAACCGTGCGCGATTCCGGGCGAGGCATT
CCCTTAGGGAAAGTGATCGATTGCGTTTCTAAGATCAATACCGGAGCAAAGTATACTCAAGATGTATTTCATTTTTCTGT
AGGATTAAATGGCGTGGGGTTAAAAGCTGTTAACGCCCTTTCAGAAACGTTTACTGTACGCTCCGTACGCAAGAAACAAT
ACCACTATGCAACCTTTTGCAAGGGAATTTTGCAAAATTCGCATCAAGGTTCTACGAAAGATCCAGACGGGACAGAAATC
ACCTTTTCTCCTGATGCCACAATCTTTACCAACTTCTCTTTTAATGATGAATTTTTAAGAAAGAAAATCCGTCGTTATAC
TTATTTGCATCCTGGTCTTGAGATTATTTGCAATAATGAAGTTTTCCTATCGCAACAAGGTCTTTTAGACTTATTTAAAG
AGGAAGTTCCTGAAGATACCCTTTACCCGCCAATAACCTTCCAAAATTCTGAATTATCCTTTCTATTCACACATGCAGAA
ACACATAACGAACGTTATTTTTCTTTTGTTAACGGTCAAGAAACTATAGATGGGGGTACCCATCTAGCAGCTTTTAAAGA
GGCTATTGTTAAAGGAATCAACGAGTATTTTGGAAAAAGTTTTGCAAGCAATGATATTCGCAATGGCCTTATCGGCTGTA
TCGCAATAAAAATTGCCTCGCCAATTTTTGAATCACAAACAAAGAATAAGCTTGGCAACACACAAATTCGCCCAGGAATA
ATTAAAGACGTTAAAAGCGCGATTATTCAAGAGCTCAAAAAAAACAAACCCTATGCTGATCTTCTTCTTAATAAGATTAA
GCTAAATGAAAAAACCCGAAAAAATATTCAATTCCTCAAACAGGATCTCAAGGATAAACAAAAAAAACTCCATTATAAGA
TCCCAAAACTTCGTGATTGTAAGTTTCACTACAACGAGCGTTCTCTATATGGCGAGGCATCATCTATTTTCGTGACGGAG
GGAGAATCTGCTTCTGCATCTATTTTATCTTCAAGAAATCCACTAACCCAAGCTGTCTTTTCTCTTCGAGGGAAACCCCT
GAACGTTTTTTCCCTAGAAGAGGAGAAAATGTATAAAAATGACGAGCTATTCTACTTGGCTACAGCATTAGGTATTACGA
AAAATAGTACGCAGCTCTTGCGCTACAATAAAATTATTCTTGCTACTGATGCTGATGTGGACGGGATGCATATCCGTAAT
TTATTAATTACATTCTTTTTAAAAACCTTCTTACCAATAGTTGAAAACCGTCACTTGTTTATCTTAGAAACGCCTGTATT
CAAAGTTCGTTATAAAGAGACGACGCTATATTGCTATTCAGAACAAGAAAAAACAGACGCTATACGGAAGTTAAGGAAAA
AAGAAACAGCATTAGAAGTAACAAGATTTAAAGGTTTAGGAGAAATTTCTCCTAAAGAATTTAAAACCTTTATTGGTGCG
GACATGCGTCTAACCCCAGTAACAATTAATGCATTAGAATCCGTAGAGACTCTTTTACAATTTTATATGGGGAAAAATAC
CAAAGAACGAAAACAATTTATTATGGACAACCTCATTACCAACTTATAG

Upstream 100 bases:

>100_bases
ACTTATAGCACTGCTATGGCTAACCATTAACTCAAACATCGCGCAGTAGAATTCTAACAGAAGTCTTTTTCCTTAGAACG
CAGTAAGAAGAATAGAAAAT

Downstream 100 bases:

>100_bases
CTTATGCAGGACGTTTCAGATCTTTTTAAGACGCATTTTATGCACTACGCATCTTATGTGATTTTAGAAAGAGCCATTCC
CCACATTCTTGATGGTCTTA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MATYTEASVVSLASLEHIRLRAGMYIGRLGDGSQIEDGIYTLFKEVVDNAIDEFIMGHGKTISISTNATTITVRDSGRGI
PLGKVIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNALSETFTVRSVRKKQYHYATFCKGILQNSHQGSTKDPDGTEI
TFSPDATIFTNFSFNDEFLRKKIRRYTYLHPGLEIICNNEVFLSQQGLLDLFKEEVPEDTLYPPITFQNSELSFLFTHAE
THNERYFSFVNGQETIDGGTHLAAFKEAIVKGINEYFGKSFASNDIRNGLIGCIAIKIASPIFESQTKNKLGNTQIRPGI
IKDVKSAIIQELKKNKPYADLLLNKIKLNEKTRKNIQFLKQDLKDKQKKLHYKIPKLRDCKFHYNERSLYGEASSIFVTE
GESASASILSSRNPLTQAVFSLRGKPLNVFSLEEEKMYKNDELFYLATALGITKNSTQLLRYNKIILATDADVDGMHIRN
LLITFFLKTFLPIVENRHLFILETPVFKVRYKETTLYCYSEQEKTDAIRKLRKKETALEVTRFKGLGEISPKEFKTFIGA
DMRLTPVTINALESVETLLQFYMGKNTKERKQFIMDNLITNL

Sequences:

>Translated_602_residues
MATYTEASVVSLASLEHIRLRAGMYIGRLGDGSQIEDGIYTLFKEVVDNAIDEFIMGHGKTISISTNATTITVRDSGRGI
PLGKVIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNALSETFTVRSVRKKQYHYATFCKGILQNSHQGSTKDPDGTEI
TFSPDATIFTNFSFNDEFLRKKIRRYTYLHPGLEIICNNEVFLSQQGLLDLFKEEVPEDTLYPPITFQNSELSFLFTHAE
THNERYFSFVNGQETIDGGTHLAAFKEAIVKGINEYFGKSFASNDIRNGLIGCIAIKIASPIFESQTKNKLGNTQIRPGI
IKDVKSAIIQELKKNKPYADLLLNKIKLNEKTRKNIQFLKQDLKDKQKKLHYKIPKLRDCKFHYNERSLYGEASSIFVTE
GESASASILSSRNPLTQAVFSLRGKPLNVFSLEEEKMYKNDELFYLATALGITKNSTQLLRYNKIILATDADVDGMHIRN
LLITFFLKTFLPIVENRHLFILETPVFKVRYKETTLYCYSEQEKTDAIRKLRKKETALEVTRFKGLGEISPKEFKTFIGA
DMRLTPVTINALESVETLLQFYMGKNTKERKQFIMDNLITNL
>Mature_601_residues
ATYTEASVVSLASLEHIRLRAGMYIGRLGDGSQIEDGIYTLFKEVVDNAIDEFIMGHGKTISISTNATTITVRDSGRGIP
LGKVIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNALSETFTVRSVRKKQYHYATFCKGILQNSHQGSTKDPDGTEIT
FSPDATIFTNFSFNDEFLRKKIRRYTYLHPGLEIICNNEVFLSQQGLLDLFKEEVPEDTLYPPITFQNSELSFLFTHAET
HNERYFSFVNGQETIDGGTHLAAFKEAIVKGINEYFGKSFASNDIRNGLIGCIAIKIASPIFESQTKNKLGNTQIRPGII
KDVKSAIIQELKKNKPYADLLLNKIKLNEKTRKNIQFLKQDLKDKQKKLHYKIPKLRDCKFHYNERSLYGEASSIFVTEG
ESASASILSSRNPLTQAVFSLRGKPLNVFSLEEEKMYKNDELFYLATALGITKNSTQLLRYNKIILATDADVDGMHIRNL
LITFFLKTFLPIVENRHLFILETPVFKVRYKETTLYCYSEQEKTDAIRKLRKKETALEVTRFKGLGEISPKEFKTFIGAD
MRLTPVTINALESVETLLQFYMGKNTKERKQFIMDNLITNL

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=612, Percent_Identity=27.4509803921569, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI19913406, Length=622, Percent_Identity=25.5627009646302, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=31.3725490196078, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789408, Length=638, Percent_Identity=27.8996865203762, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17535065, Length=606, Percent_Identity=24.7524752475248, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=25.8333333333333, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324241, Length=625, Percent_Identity=24.64, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17136538, Length=660, Percent_Identity=25.6060606060606, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3

Molecular weight: Translated: 68298; Mature: 68167

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYTEASVVSLASLEHIRLRAGMYIGRLGDGSQIEDGIYTLFKEVVDNAIDEFIMGHGK
CCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TISISTNATTITVRDSGRGIPLGKVIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNAL
EEEEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCHHHHHHH
SETFTVRSVRKKQYHYATFCKGILQNSHQGSTKDPDGTEITFSPDATIFTNFSFNDEFLR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHH
KKIRRYTYLHPGLEIICNNEVFLSQQGLLDLFKEEVPEDTLYPPITFQNSELSFLFTHAE
HHHHHHHEECCCCEEEECCCEEECCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEECC
THNERYFSFVNGQETIDGGTHLAAFKEAIVKGINEYFGKSFASNDIRNGLIGCIAIKIAS
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHH
PIFESQTKNKLGNTQIRPGIIKDVKSAIIQELKKNKPYADLLLNKIKLNEKTRKNIQFLK
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
QDLKDKQKKLHYKIPKLRDCKFHYNERSLYGEASSIFVTEGESASASILSSRNPLTQAVF
HHHHHHHHHHHCCCCCCCCCEEECCCHHHCCCCCEEEEECCCCCCHHHHHCCCHHHHHHH
SLRGKPLNVFSLEEEKMYKNDELFYLATALGITKNSTQLLRYNKIILATDADVDGMHIRN
HHCCCCCEEEECCHHHHCCCCCEEEEEEECCCCCCCCEEEEECEEEEEECCCCCCHHHHH
LLITFFLKTFLPIVENRHLFILETPVFKVRYKETTLYCYSEQEKTDAIRKLRKKETALEV
HHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHH
TRFKGLGEISPKEFKTFIGADMRLTPVTINALESVETLLQFYMGKNTKERKQFIMDNLIT
HHHCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NL
CC
>Mature Secondary Structure 
ATYTEASVVSLASLEHIRLRAGMYIGRLGDGSQIEDGIYTLFKEVVDNAIDEFIMGHGK
CCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TISISTNATTITVRDSGRGIPLGKVIDCVSKINTGAKYTQDVFHFSVGLNGVGLKAVNAL
EEEEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCHHHHHHH
SETFTVRSVRKKQYHYATFCKGILQNSHQGSTKDPDGTEITFSPDATIFTNFSFNDEFLR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEECCCCHHHHH
KKIRRYTYLHPGLEIICNNEVFLSQQGLLDLFKEEVPEDTLYPPITFQNSELSFLFTHAE
HHHHHHHEECCCCEEEECCCEEECCHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEEECC
THNERYFSFVNGQETIDGGTHLAAFKEAIVKGINEYFGKSFASNDIRNGLIGCIAIKIAS
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHH
PIFESQTKNKLGNTQIRPGIIKDVKSAIIQELKKNKPYADLLLNKIKLNEKTRKNIQFLK
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
QDLKDKQKKLHYKIPKLRDCKFHYNERSLYGEASSIFVTEGESASASILSSRNPLTQAVF
HHHHHHHHHHHCCCCCCCCCEEECCCHHHCCCCCEEEEECCCCCCHHHHHCCCHHHHHHH
SLRGKPLNVFSLEEEKMYKNDELFYLATALGITKNSTQLLRYNKIILATDADVDGMHIRN
HHCCCCCEEEECCHHHHCCCCCEEEEEEECCCCCCCCEEEEECEEEEEECCCCCCHHHHH
LLITFFLKTFLPIVENRHLFILETPVFKVRYKETTLYCYSEQEKTDAIRKLRKKETALEV
HHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHH
TRFKGLGEISPKEFKTFIGADMRLTPVTINALESVETLLQFYMGKNTKERKQFIMDNLIT
HHHCCCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]