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The map label for this gene is dagA2 [H]

Identifier: 89898605

GI number: 89898605

Start: 938906

End: 940261

Strand: Direct

Name: dagA2 [H]

Synonym: CF0798

Alternate gene names: 89898605

Gene position: 938906-940261 (Clockwise)

Preceding gene: 89898604

Following gene: 89898606

Centisome position: 80.51

GC content: 42.33

Gene sequence:

>1356_bases
ATGAACGCAATCCTATCTTTGCTAGCAGCCTTTGACGACTTCTTTTGGTCCTACGTGGCTTTTTTCATGATCATAGTTCT
AGGGCTAAGTTTCTCATGGAAATCGAGATTTTCCCAATTCACCAAGTTCCCACAATTTTGCAGACTTTTTTATCAGTACT
CTAAGGAATCTTCCAATAGGACAGGTAAAGAAGGTGAAAGAGGGGTTCATCCGTTAAAAGTATTTTTTGCCTCTGCAAGT
GGAAATATCGGTATTGGTAATGTTGTCGGGATTGTTACTGCGGCGTGTATTGGTGGTCCCGGAGCCCTCTTTTGGGTTTG
GATTGCAGGAATTTTCGGCTCCATAGTTAAGTACGCTGAGGTTTACTTAGGGATTAAATTCCGCAAAGTGGATAACGACG
GTGTTTATCAAGGTGGACCTATGTACTTCCTGAATAAGGCATACGGTACAAAACTCGTCCCTATTATTGTTGCTGTTTTG
CTCTGTATTTATGGTGTGGAGATCTATCAGTTTTCTGTTATTGCTGACACGATTTCACACTGCTGGAGTATCCCAAAACT
GTTCACAATTTTTGGTCTACTCTTTCTCATTCTCTATGCAGTTCGTGGAGGATTACAGAGAATTGGAAAAATCTGTGCTT
TTGTACTCCCATTTTTCCTCACTATATACTGCGCACTATCTCTATACATACTTATAAGAGAGTTCCACCAGATTCCAGCC
TTATTTTCTTCTGTATTTTCATCAGCCTTTACAGGGCATGGAGCTATCGGAGGATTTGCGGGGTGCACTTTAGCCACGAC
AATTCACCAAGGGATTTCTCGAGCTGCTTATTCTGGAGACATTGGCATTGGATTTGACTCTATTATTCAAAGTGAGAGTT
CTGCTAAGAAACCAGAAACTCAAGCACAGTTAAGCATCATAGGCCTCGCCATAGACAACCTTATCTGCACGCTAAGCCTA
CTTACGGTTCTCGCTTCAGGATCCTGGTCTCTGGGACTTGACAACGCCTCCCAAGCTGTGGAACACGCTTTAGCAACTTA
CTTCCCGTTTGTGAAAATCTTACTCCCCACTTTCTTCTTTGTCACAGGTTATACGACTATCATTTCGTATTTCCTCGTTG
GGAAAAAGTGTGCAAAGTTTCTTTACGGGAATACCGGGTCGAAAATCTATACTGTCTACGGAGCCACCATGCTACCCGCG
TTTTGTTTCCTATCGCAAAACACTGCTTTGTTGATTATGTCCGTATCTGGAGCTCTTCTCTTATGCTTCAATCTATTCGG
GGTCTTTCTCATGAGAAAGGAGATAGAATTCCCCAAATCTGACAAAGTTATAGAGATTCCCTCTGTGGCAGAATAA

Upstream 100 bases:

>100_bases
AAGCTTTATTTTCCTACTAAACAAAAGATAAAATAGGGATAATAAATACCCTATCTTTCTGAAGAATAACTGTTTTTACC
CCAATAAAAAAAGTTTTACC

Downstream 100 bases:

>100_bases
AAATTTTGGGGGCTTTTCCCTAATCCTATTGCATTCATTATCAGCTACATATCCCCAACGCCTGTTGGGGATTTTATTTT
CTATATCCCTAACCTGCTTA

Product: D-alanine/glycine permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNAILSLLAAFDDFFWSYVAFFMIIVLGLSFSWKSRFSQFTKFPQFCRLFYQYSKESSNRTGKEGERGVHPLKVFFASAS
GNIGIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYAEVYLGIKFRKVDNDGVYQGGPMYFLNKAYGTKLVPIIVAVL
LCIYGVEIYQFSVIADTISHCWSIPKLFTIFGLLFLILYAVRGGLQRIGKICAFVLPFFLTIYCALSLYILIREFHQIPA
LFSSVFSSAFTGHGAIGGFAGCTLATTIHQGISRAAYSGDIGIGFDSIIQSESSAKKPETQAQLSIIGLAIDNLICTLSL
LTVLASGSWSLGLDNASQAVEHALATYFPFVKILLPTFFFVTGYTTIISYFLVGKKCAKFLYGNTGSKIYTVYGATMLPA
FCFLSQNTALLIMSVSGALLLCFNLFGVFLMRKEIEFPKSDKVIEIPSVAE

Sequences:

>Translated_451_residues
MNAILSLLAAFDDFFWSYVAFFMIIVLGLSFSWKSRFSQFTKFPQFCRLFYQYSKESSNRTGKEGERGVHPLKVFFASAS
GNIGIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYAEVYLGIKFRKVDNDGVYQGGPMYFLNKAYGTKLVPIIVAVL
LCIYGVEIYQFSVIADTISHCWSIPKLFTIFGLLFLILYAVRGGLQRIGKICAFVLPFFLTIYCALSLYILIREFHQIPA
LFSSVFSSAFTGHGAIGGFAGCTLATTIHQGISRAAYSGDIGIGFDSIIQSESSAKKPETQAQLSIIGLAIDNLICTLSL
LTVLASGSWSLGLDNASQAVEHALATYFPFVKILLPTFFFVTGYTTIISYFLVGKKCAKFLYGNTGSKIYTVYGATMLPA
FCFLSQNTALLIMSVSGALLLCFNLFGVFLMRKEIEFPKSDKVIEIPSVAE
>Mature_451_residues
MNAILSLLAAFDDFFWSYVAFFMIIVLGLSFSWKSRFSQFTKFPQFCRLFYQYSKESSNRTGKEGERGVHPLKVFFASAS
GNIGIGNVVGIVTAACIGGPGALFWVWIAGIFGSIVKYAEVYLGIKFRKVDNDGVYQGGPMYFLNKAYGTKLVPIIVAVL
LCIYGVEIYQFSVIADTISHCWSIPKLFTIFGLLFLILYAVRGGLQRIGKICAFVLPFFLTIYCALSLYILIREFHQIPA
LFSSVFSSAFTGHGAIGGFAGCTLATTIHQGISRAAYSGDIGIGFDSIIQSESSAKKPETQAQLSIIGLAIDNLICTLSL
LTVLASGSWSLGLDNASQAVEHALATYFPFVKILLPTFFFVTGYTTIISYFLVGKKCAKFLYGNTGSKIYTVYGATMLPA
FCFLSQNTALLIMSVSGALLLCFNLFGVFLMRKEIEFPKSDKVIEIPSVAE

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=407, Percent_Identity=26.7813267813268, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49394; Mature: 49394

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]