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The map label for this gene is dnaN [H]

Identifier: 89898369

GI number: 89898369

Start: 659413

End: 660513

Strand: Direct

Name: dnaN [H]

Synonym: CF0562

Alternate gene names: 89898369

Gene position: 659413-660513 (Clockwise)

Preceding gene: 89898367

Following gene: 89898370

Centisome position: 56.54

GC content: 39.24

Gene sequence:

>1101_bases
ATGAAGTTCGTCGTATCCCGAAATGAGTTAGGAAACCTTATAAAAAAAATTCAAAGCGTTGTTCCTCAAAATACTCCTAT
CCCTGTGCTTACTCATGTACTTATTGAAACATGCAATGACGAGCTCGTTTTTACTGCTACGGATCTTACTGTAAGCACCC
GCTGTGTAGCCCAAGCAAAAGTTTATGAATCAGGAGCTATTTCTATTCCTTCTAAAAGATTTTTCCAGCTAGTGAAGGAA
TTAACAGAGGCGAATCTTGAAATCTCTGCAACCACCGGAGAAATGGCAAAAATTACCTCGGGATCTTCTTGCTTCCGTCT
ACTAAGTATGGGGAAAGAAGACTTCCCTATGCTTCCTGACATCCAAAATTCTATAAGATTTACTCTCCCCGCAGAACAGC
TTAAGGAAATGCTACAGCGTACTTCATTCGCTGTTTCCCGAGAAGAAAGTCGTTATGTTCTTACTGGGGTTTTATTAACG
ATTGCGGATGGAATGGTTACCGTAGTGGGTACAGACGGGAAACGGTTAGCAAAAACTGATACAGAAATTTCTTTAGATAA
AAGCTTTGCTGGTGACTATATCGTTCCTATTAAAGCTGTCGAAGAAATGATTAAGCTCTGTTCTGAAGACTCTGAAGCTA
CAATCTTCTTAGATCAAGCCAAAATTGCTGTAGAATGCGGCAATACATTGTTAATTACAAAATTACTTTCTGGAGAATTT
CCTGACTTCTCTCCTGTAATCTCTACAGAAAGCAGTGTGCAGCTAGATCTTCATAGGGAAGAACTCATTACCCTATTAAA
ACAGGTAGCTTTATTTACTAACGAGTCTTCTCATTCTGTGAAATTCACCTTTACTCCTGGAGAGCTCACCCTAACAGCTA
ACTGTACGAAAGTCGGCGAAGGGAAGGTCAGCATGGCAGTAAATTATTCCGGAGAATTACTGGAAATTGCATTTAATCCT
TTCTTCTTCCTCGATATCTTAAAGCATAGTAAAGATGAGTTAGTTCGATTAGGCATTTCAGACTCTTATAATCCTGGGAT
TATTACTGATTCCACACGCAGCCTATTCGTTATTATGCCAATGAGATTGCATGATGATTAA

Upstream 100 bases:

>100_bases
CCTGCTTTATCCTAAGGAGGCTCCAGCGAAGAGATCCCGAGGATCCCCCTATTCTCTTAGGGGCAAGATTGAATTTAATG
GCTTACAGTTGTAGGAAAAT

Downstream 100 bases:

>100_bases
TGAATATTCTTTCCCTGCGTCTTAAGAATTTCAGAAATTATAAAGAAGCCAAGATTTCTTTATCTCCAAATATGAATTAT
ATTTTTGGAGAAAATGCTCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETCNDELVFTATDLTVSTRCVAQAKVYESGAISIPSKRFFQLVKE
LTEANLEISATTGEMAKITSGSSCFRLLSMGKEDFPMLPDIQNSIRFTLPAEQLKEMLQRTSFAVSREESRYVLTGVLLT
IADGMVTVVGTDGKRLAKTDTEISLDKSFAGDYIVPIKAVEEMIKLCSEDSEATIFLDQAKIAVECGNTLLITKLLSGEF
PDFSPVISTESSVQLDLHREELITLLKQVALFTNESSHSVKFTFTPGELTLTANCTKVGEGKVSMAVNYSGELLEIAFNP
FFFLDILKHSKDELVRLGISDSYNPGIITDSTRSLFVIMPMRLHDD

Sequences:

>Translated_366_residues
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETCNDELVFTATDLTVSTRCVAQAKVYESGAISIPSKRFFQLVKE
LTEANLEISATTGEMAKITSGSSCFRLLSMGKEDFPMLPDIQNSIRFTLPAEQLKEMLQRTSFAVSREESRYVLTGVLLT
IADGMVTVVGTDGKRLAKTDTEISLDKSFAGDYIVPIKAVEEMIKLCSEDSEATIFLDQAKIAVECGNTLLITKLLSGEF
PDFSPVISTESSVQLDLHREELITLLKQVALFTNESSHSVKFTFTPGELTLTANCTKVGEGKVSMAVNYSGELLEIAFNP
FFFLDILKHSKDELVRLGISDSYNPGIITDSTRSLFVIMPMRLHDD
>Mature_366_residues
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETCNDELVFTATDLTVSTRCVAQAKVYESGAISIPSKRFFQLVKE
LTEANLEISATTGEMAKITSGSSCFRLLSMGKEDFPMLPDIQNSIRFTLPAEQLKEMLQRTSFAVSREESRYVLTGVLLT
IADGMVTVVGTDGKRLAKTDTEISLDKSFAGDYIVPIKAVEEMIKLCSEDSEATIFLDQAKIAVECGNTLLITKLLSGEF
PDFSPVISTESSVQLDLHREELITLLKQVALFTNESSHSVKFTFTPGELTLTANCTKVGEGKVSMAVNYSGELLEIAFNP
FFFLDILKHSKDELVRLGISDSYNPGIITDSTRSLFVIMPMRLHDD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=371, Percent_Identity=30.9973045822102, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40400; Mature: 40400

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETCNDELVFTATDLTVSTRCVAQAK
CEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHH
VYESGAISIPSKRFFQLVKELTEANLEISATTGEMAKITSGSSCFRLLSMGKEDFPMLPD
EECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHCCCCCCCCCCC
IQNSIRFTLPAEQLKEMLQRTSFAVSREESRYVLTGVLLTIADGMVTVVGTDGKRLAKTD
CCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCC
TEISLDKSFAGDYIVPIKAVEEMIKLCSEDSEATIFLDQAKIAVECGNTLLITKLLSGEF
CCEEECCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCEEEEEEHHCCCC
PDFSPVISTESSVQLDLHREELITLLKQVALFTNESSHSVKFTFTPGELTLTANCTKVGE
CCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCEECCC
GKVSMAVNYSGELLEIAFNPFFFLDILKHSKDELVRLGISDSYNPGIITDSTRSLFVIMP
CEEEEEEECCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCEEEEEEE
MRLHDD
EEECCC
>Mature Secondary Structure
MKFVVSRNELGNLIKKIQSVVPQNTPIPVLTHVLIETCNDELVFTATDLTVSTRCVAQAK
CEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHH
VYESGAISIPSKRFFQLVKELTEANLEISATTGEMAKITSGSSCFRLLSMGKEDFPMLPD
EECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHHHHHCCCCCCCCCCC
IQNSIRFTLPAEQLKEMLQRTSFAVSREESRYVLTGVLLTIADGMVTVVGTDGKRLAKTD
CCCCEEEEECHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCEEEEEECCCCEEECCC
TEISLDKSFAGDYIVPIKAVEEMIKLCSEDSEATIFLDQAKIAVECGNTLLITKLLSGEF
CCEEECCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCEEEEEEHHCCCC
PDFSPVISTESSVQLDLHREELITLLKQVALFTNESSHSVKFTFTPGELTLTANCTKVGE
CCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECCEECCC
GKVSMAVNYSGELLEIAFNPFFFLDILKHSKDELVRLGISDSYNPGIITDSTRSLFVIMP
CEEEEEEECCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCEEEEEEE
MRLHDD
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]