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The map label for this gene is yojL [H]

Identifier: 89898367

GI number: 89898367

Start: 657793

End: 658737

Strand: Direct

Name: yojL [H]

Synonym: CF0560

Alternate gene names: 89898367

Gene position: 657793-658737 (Clockwise)

Preceding gene: 89898366

Following gene: 89898369

Centisome position: 56.4

GC content: 39.89

Gene sequence:

>945_bases
ATGGGAAAGTTATCAAAAATCCTCCTCATAACTCTCTTATCCTGGATTCTTCAGGGATGTTCTCCTAAGCTTACAACTTT
AGAAGGCGAAAGGATGACCATACCCTACCGCATCGTCATAGGAAAACGTCTATCAAACCAGGAGACAGAACAGTTAAAAA
AAGAAGTCAATACAGTATTTCATGTCATCGATACTATTTATAATAACTGGAATAGGGAATCAGAATTATCTAAAATCAAT
CGATCTCCTGCAAATGTAGCGATTCCCCTCTCTAAGGAATTATTAGCATTCCTAAAACAAGTAGATACATTTTACCAATT
ATCAGGAGGAAGATTCGACCCCACCTTGGGTCCATTAAAAAATCTGTGGCTATTGCATCTTAAACAACATTCTCTCCCCA
ATGAGCAAACTTGGAAAACCTATTATGAAAACACAGGGTGGGAGCATATCTCAATAGATTTTACTAACCAAACAATTACG
AAACAACATGCTGATATACAGCTTGACCTTTGCGGCGCCGTAAAAGGTTTCGCTGTTGACTGTCTATTAGAAACTTGTCA
GAGATTTTGCACGAATAGTTATGCTGAGTGGGGAGGTGAAATTAAAACCTCTGGACGCCACCCCACAGGAAGACCCTGGC
GTATAGCTTCCTCTGCAACAACAAAAATCATAGAACTCAATAACACTGCAGTTGCTACAAGCGGAAACTACCACCAACAA
TGGTCTATAGAAGGGAAGATCTATACTCATATCCTAGATCCTCGTACAGGGAAACCCCTAGAACTTCACGATTATCCCAT
AGTATCCGCAACTGTTATTCACTCCAGTTGCGCTTTTGCAGATGCCATGGCGACAGTACTCATGACATTTTCAACAAAAG
CGGAAGCTTTAGCTTGGGCTCAAAAAAATAATATAGAAGCTTTTATCAACGATAACGCTTCATAG

Upstream 100 bases:

>100_bases
GAGATGTAGACTTTAATAATGTTGTCACCAAGTGTAAAGCAATCACTCCTGTTCCCGGAGGTGTCGGACCTATGACAGTG
GCCATGCTAATGAAAAATAC

Downstream 100 bases:

>100_bases
CAGATTCGACTTCTCGTTGCTTCTCCCTCGCTATAATCGTTTGTCGCTTATCGTGAGCTTTCTTACCACGACAACAACCA
AGACGAATTTTCACATAACC

Product: thiamine biosynthesis lipoprotein apbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MGKLSKILLITLLSWILQGCSPKLTTLEGERMTIPYRIVIGKRLSNQETEQLKKEVNTVFHVIDTIYNNWNRESELSKIN
RSPANVAIPLSKELLAFLKQVDTFYQLSGGRFDPTLGPLKNLWLLHLKQHSLPNEQTWKTYYENTGWEHISIDFTNQTIT
KQHADIQLDLCGAVKGFAVDCLLETCQRFCTNSYAEWGGEIKTSGRHPTGRPWRIASSATTKIIELNNTAVATSGNYHQQ
WSIEGKIYTHILDPRTGKPLELHDYPIVSATVIHSSCAFADAMATVLMTFSTKAEALAWAQKNNIEAFINDNAS

Sequences:

>Translated_314_residues
MGKLSKILLITLLSWILQGCSPKLTTLEGERMTIPYRIVIGKRLSNQETEQLKKEVNTVFHVIDTIYNNWNRESELSKIN
RSPANVAIPLSKELLAFLKQVDTFYQLSGGRFDPTLGPLKNLWLLHLKQHSLPNEQTWKTYYENTGWEHISIDFTNQTIT
KQHADIQLDLCGAVKGFAVDCLLETCQRFCTNSYAEWGGEIKTSGRHPTGRPWRIASSATTKIIELNNTAVATSGNYHQQ
WSIEGKIYTHILDPRTGKPLELHDYPIVSATVIHSSCAFADAMATVLMTFSTKAEALAWAQKNNIEAFINDNAS
>Mature_313_residues
GKLSKILLITLLSWILQGCSPKLTTLEGERMTIPYRIVIGKRLSNQETEQLKKEVNTVFHVIDTIYNNWNRESELSKINR
SPANVAIPLSKELLAFLKQVDTFYQLSGGRFDPTLGPLKNLWLLHLKQHSLPNEQTWKTYYENTGWEHISIDFTNQTITK
QHADIQLDLCGAVKGFAVDCLLETCQRFCTNSYAEWGGEIKTSGRHPTGRPWRIASSATTKIIELNNTAVATSGNYHQQW
SIEGKIYTHILDPRTGKPLELHDYPIVSATVIHSSCAFADAMATVLMTFSTKAEALAWAQKNNIEAFINDNAS

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=280, Percent_Identity=26.7857142857143, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 35412; Mature: 35281

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLSKILLITLLSWILQGCSPKLTTLEGERMTIPYRIVIGKRLSNQETEQLKKEVNTVF
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHH
HVIDTIYNNWNRESELSKINRSPANVAIPLSKELLAFLKQVDTFYQLSGGRFDPTLGPLK
HHHHHHHCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
NLWLLHLKQHSLPNEQTWKTYYENTGWEHISIDFTNQTITKQHADIQLDLCGAVKGFAVD
HHHHHEEHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHCCCEEEEECCCHHHHHHH
CLLETCQRFCTNSYAEWGGEIKTSGRHPTGRPWRIASSATTKIIELNNTAVATSGNYHQQ
HHHHHHHHHHCCCHHHCCCCEECCCCCCCCCCEEEECCCCEEEEEECCEEEEECCCCCEE
WSIEGKIYTHILDPRTGKPLELHDYPIVSATVIHSSCAFADAMATVLMTFSTKAEALAWA
EEECCEEEEEEECCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QKNNIEAFINDNAS
HCCCEEEEECCCCC
>Mature Secondary Structure 
GKLSKILLITLLSWILQGCSPKLTTLEGERMTIPYRIVIGKRLSNQETEQLKKEVNTVF
CHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHH
HVIDTIYNNWNRESELSKINRSPANVAIPLSKELLAFLKQVDTFYQLSGGRFDPTLGPLK
HHHHHHHCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
NLWLLHLKQHSLPNEQTWKTYYENTGWEHISIDFTNQTITKQHADIQLDLCGAVKGFAVD
HHHHHEEHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHCCCEEEEECCCHHHHHHH
CLLETCQRFCTNSYAEWGGEIKTSGRHPTGRPWRIASSATTKIIELNNTAVATSGNYHQQ
HHHHHHHHHHCCCHHHCCCCEECCCCCCCCCCEEEECCCCEEEEEECCEEEEECCCCCEE
WSIEGKIYTHILDPRTGKPLELHDYPIVSATVIHSSCAFADAMATVLMTFSTKAEALAWA
EEECCEEEEEEECCCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
QKNNIEAFINDNAS
HCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]