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The map label for this gene is ligA

Identifier: 89898194

GI number: 89898194

Start: 453064

End: 455052

Strand: Direct

Name: ligA

Synonym: CF0387

Alternate gene names: 89898194

Gene position: 453064-455052 (Clockwise)

Preceding gene: 89898193

Following gene: 89898195

Centisome position: 38.85

GC content: 42.28

Gene sequence:

>1989_bases
ATGCACAATATGTATACCAGGGAACAATATTTAGCTCTGTGTAAAGATATCGAAGAAGATGATTACCGCTATTATGTTCT
TCATGATCCTATAATCTCGGATTATGACTATGATATGAAAATGCAAAAGCTTCTTTCTATAGAGGCTCAGCATCCTGAAT
GGCGGGTATTGTGGTCACCGTCTATGCGTCTTGGAGATCGCATCTCGGGAAGTTTCCCCGTTGTTACGCACTCTCACCCC
ATGCTCTCTATTGCTAATGCCTATACTTTAGAAGAGCTTAATGATTTCTTTTCCCGTGTAGAGAAAACTTTAGGGTATAC
CCCTACATATACCCTAGAGCTAAAAATTGACGGTATTGCCGTAGCTATTCGTTATGAACAGAGGGTCCTTGTTCAAGCTT
TAAGTCGTGGGAACGGACGTCAAGGAGAGGATATTACAACAAATATCCGCACAATACGCTCTTTGCCTTTGCGCTTACCA
AAAGAGGCTCCGGAATTTCTTGAAGTTCGTGGAGAAGTGTTTTTTACGCGAGCAACTTTTGAACAGATAAACTCTATACA
AAGGCAGTCAGAAAAACCGGAATTTGCTAATCCCCGAAATGCTGCAGGGGGTACTCTGAAGCTTTTATCTGCCAAAGAAG
CTGCTCAAAGAAATTTAGAGCTCTCTATTTATGGGGCTTGGGCAGAGGAGAATACGGAATCGCATTATGACAATCTTATG
TTGTGTCAGAAATGGGGGTTCCCTGTTTTTGGACGGCCACGACAATATAAGAGTGTAGAGGAAGTTGTAAAAGCTCTTCA
TGAGATAGCGAAGATCCGAGATCAGTTACCTATGGAAATCGACGGTGTAGTGATCAAAGTGGATAACACCGAAGATCAAA
AAGTTTTGGGAATGACAGCGAAACATTATCGCTGGGCGTTAGCGTATAAATATGCTCCTGAACAGGGAGAAACTATTTTA
GAAGACATTCTCATTCAGGTCGGTAGGACAGGAGTATTAACTCCTGTGGCTAAGTTGCGTCCTGTATTTTTGTCGGGGTC
GAAAGTTTCTCGAGCTTCTTTATATAATGAAGAAGAAATCGAAAGAAAAGACATTCGTATTGGGGATACCGTATACGTAG
AAAAAGGCGGGGAAATTATTCCTAAAGTTGTTGGAGTATGTTTGGAAAAGCGCCCGGAGGGTACGAAGCCCTGGGTTATG
CCAGAATACTGTCCCGTTTGCCACGAAAGGGTATCTCGGGAATCCGATAAGGTTTCCGTACGCTGTACAAATTCTTCTTG
TTCTGCGGGAACTATAGAAAAAATTCGCTTTTTTGTGGGCAGAGAAGCTCTAGATATCGAGCATCTTGGGGAAAAGGTGA
TCACAAAGCTATTTGATCTAGGATTAATTCATCGACGCTGTGACATTTTTCAAATTACTGAGGAGGACCTTCTTCAAGTC
CCGGGTTTCAAGGATAAATCCATAAAAAATGTTCTTAAAAGTATTGAGAAGGCTAAAAGTGCTCCGTTAGAGCGTTTTAT
TGCAGCGCTTGGCATTCCCTATGTGGGGATTGGCGGCGCTAATGCTCTTGCTCAACACTTTCTCTCTTTGGATGCTGTGA
TGAGCGCTTCTTTAGAAGAACTGAAGGTTATAGATGGAATTGGATCTAAGGTTGCGGAGTCTATAGTAGACTACTTCCAT
CAATCAGACAATGTCGAAGAGATCCAGAAAATGCTTTCCCTAGGGGTGAATGTGCTTCCATACTCTAGGGCAAGCGCTTC
TTGCCAAGGGAAAACCTTTGTGATTACTGGAAGTCTTGAAAAAATGACCAGGTCTGAAGCCGAGGCTTCTATTCGTAATT
GTGGAGGGAAAGTCGGTTCTTCCGTATCCAAAAGCATCAATTATCTCGTCGTGGGGAAAGATCCTGGATCTAAATTAAAA
AAGGCCCAGGAGCTCCAAATCCCCATTCTTAATGAGAGTGATTTATTAAAAATCCTTTACCCAAACTAA

Upstream 100 bases:

>100_bases
AAAGATGATCTTCGCTGTGCTCATCGTCACCGAAATAATCCCGGTGCGGTAAATAGTACCTACGGATTCCGCTGCGCAAA
AGGAGTTAAGTAGACACTGT

Downstream 100 bases:

>100_bases
TCCCCTCTTAGCTTTTTCTATTTTTGTTAAGTTCTTTACCTTAACTATTAAATAAATAGCAAAGGTGTTCTGTTCTTATA
TTATTTAATATAAATTCTTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MHNMYTREQYLALCKDIEEDDYRYYVLHDPIISDYDYDMKMQKLLSIEAQHPEWRVLWSPSMRLGDRISGSFPVVTHSHP
MLSIANAYTLEELNDFFSRVEKTLGYTPTYTLELKIDGIAVAIRYEQRVLVQALSRGNGRQGEDITTNIRTIRSLPLRLP
KEAPEFLEVRGEVFFTRATFEQINSIQRQSEKPEFANPRNAAGGTLKLLSAKEAAQRNLELSIYGAWAEENTESHYDNLM
LCQKWGFPVFGRPRQYKSVEEVVKALHEIAKIRDQLPMEIDGVVIKVDNTEDQKVLGMTAKHYRWALAYKYAPEQGETIL
EDILIQVGRTGVLTPVAKLRPVFLSGSKVSRASLYNEEEIERKDIRIGDTVYVEKGGEIIPKVVGVCLEKRPEGTKPWVM
PEYCPVCHERVSRESDKVSVRCTNSSCSAGTIEKIRFFVGREALDIEHLGEKVITKLFDLGLIHRRCDIFQITEEDLLQV
PGFKDKSIKNVLKSIEKAKSAPLERFIAALGIPYVGIGGANALAQHFLSLDAVMSASLEELKVIDGIGSKVAESIVDYFH
QSDNVEEIQKMLSLGVNVLPYSRASASCQGKTFVITGSLEKMTRSEAEASIRNCGGKVGSSVSKSINYLVVGKDPGSKLK
KAQELQIPILNESDLLKILYPN

Sequences:

>Translated_662_residues
MHNMYTREQYLALCKDIEEDDYRYYVLHDPIISDYDYDMKMQKLLSIEAQHPEWRVLWSPSMRLGDRISGSFPVVTHSHP
MLSIANAYTLEELNDFFSRVEKTLGYTPTYTLELKIDGIAVAIRYEQRVLVQALSRGNGRQGEDITTNIRTIRSLPLRLP
KEAPEFLEVRGEVFFTRATFEQINSIQRQSEKPEFANPRNAAGGTLKLLSAKEAAQRNLELSIYGAWAEENTESHYDNLM
LCQKWGFPVFGRPRQYKSVEEVVKALHEIAKIRDQLPMEIDGVVIKVDNTEDQKVLGMTAKHYRWALAYKYAPEQGETIL
EDILIQVGRTGVLTPVAKLRPVFLSGSKVSRASLYNEEEIERKDIRIGDTVYVEKGGEIIPKVVGVCLEKRPEGTKPWVM
PEYCPVCHERVSRESDKVSVRCTNSSCSAGTIEKIRFFVGREALDIEHLGEKVITKLFDLGLIHRRCDIFQITEEDLLQV
PGFKDKSIKNVLKSIEKAKSAPLERFIAALGIPYVGIGGANALAQHFLSLDAVMSASLEELKVIDGIGSKVAESIVDYFH
QSDNVEEIQKMLSLGVNVLPYSRASASCQGKTFVITGSLEKMTRSEAEASIRNCGGKVGSSVSKSINYLVVGKDPGSKLK
KAQELQIPILNESDLLKILYPN
>Mature_662_residues
MHNMYTREQYLALCKDIEEDDYRYYVLHDPIISDYDYDMKMQKLLSIEAQHPEWRVLWSPSMRLGDRISGSFPVVTHSHP
MLSIANAYTLEELNDFFSRVEKTLGYTPTYTLELKIDGIAVAIRYEQRVLVQALSRGNGRQGEDITTNIRTIRSLPLRLP
KEAPEFLEVRGEVFFTRATFEQINSIQRQSEKPEFANPRNAAGGTLKLLSAKEAAQRNLELSIYGAWAEENTESHYDNLM
LCQKWGFPVFGRPRQYKSVEEVVKALHEIAKIRDQLPMEIDGVVIKVDNTEDQKVLGMTAKHYRWALAYKYAPEQGETIL
EDILIQVGRTGVLTPVAKLRPVFLSGSKVSRASLYNEEEIERKDIRIGDTVYVEKGGEIIPKVVGVCLEKRPEGTKPWVM
PEYCPVCHERVSRESDKVSVRCTNSSCSAGTIEKIRFFVGREALDIEHLGEKVITKLFDLGLIHRRCDIFQITEEDLLQV
PGFKDKSIKNVLKSIEKAKSAPLERFIAALGIPYVGIGGANALAQHFLSLDAVMSASLEELKVIDGIGSKVAESIVDYFH
QSDNVEEIQKMLSLGVNVLPYSRASASCQGKTFVITGSLEKMTRSEAEASIRNCGGKVGSSVSKSINYLVVGKDPGSKLK
KAQELQIPILNESDLLKILYPN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=664, Percent_Identity=40.3614457831325, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI87082305, Length=460, Percent_Identity=24.3478260869565, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI62472391, Length=90, Percent_Identity=46.6666666666667, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737765, Length=90, Percent_Identity=46.6666666666667, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHLFF (Q254X9)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515304.1
- ProteinModelPortal:   Q254X9
- SMR:   Q254X9
- STRING:   Q254X9
- GeneID:   3959017
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0387
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   CFEL264202:CF0387-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74549; Mature: 74549

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 172-172 BINDING 286-286 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNMYTREQYLALCKDIEEDDYRYYVLHDPIISDYDYDMKMQKLLSIEAQHPEWRVLWSP
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECC
SMRLGDRISGSFPVVTHSHPMLSIANAYTLEELNDFFSRVEKTLGYTPTYTLELKIDGIA
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEE
VAIRYEQRVLVQALSRGNGRQGEDITTNIRTIRSLPLRLPKEAPEFLEVRGEVFFTRATF
EEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEHHHH
EQINSIQRQSEKPEFANPRNAAGGTLKLLSAKEAAQRNLELSIYGAWAEENTESHYDNLM
HHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEEEEEECCCCCCHHHHCCEE
LCQKWGFPVFGRPRQYKSVEEVVKALHEIAKIRDQLPMEIDGVVIKVDNTEDQKVLGMTA
EEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEECCCCCCHHHCCCH
KHYRWALAYKYAPEQGETILEDILIQVGRTGVLTPVAKLRPVFLSGSKVSRASLYNEEEI
HHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCEEEECCCCCHHHHCCCHHHH
ERKDIRIGDTVYVEKGGEIIPKVVGVCLEKRPEGTKPWVMPEYCPVCHERVSRESDKVSV
HHHCEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
RCTNSSCSAGTIEKIRFFVGREALDIEHLGEKVITKLFDLGLIHRRCDIFQITEEDLLQV
EECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHC
PGFKDKSIKNVLKSIEKAKSAPLERFIAALGIPYVGIGGANALAQHFLSLDAVMSASLEE
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
LKVIDGIGSKVAESIVDYFHQSDNVEEIQKMLSLGVNVLPYSRASASCQGKTFVITGSLE
HHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCHH
KMTRSEAEASIRNCGGKVGSSVSKSINYLVVGKDPGSKLKKAQELQIPILNESDLLKILY
HHHHHHHHHHHHHCCCHHCCHHHCCCCEEEEECCCCHHHHHHHHCCCEEECCCCEEEEEC
PN
CC
>Mature Secondary Structure
MHNMYTREQYLALCKDIEEDDYRYYVLHDPIISDYDYDMKMQKLLSIEAQHPEWRVLWSP
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECC
SMRLGDRISGSFPVVTHSHPMLSIANAYTLEELNDFFSRVEKTLGYTPTYTLELKIDGIA
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCEE
VAIRYEQRVLVQALSRGNGRQGEDITTNIRTIRSLPLRLPKEAPEFLEVRGEVFFTRATF
EEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEEHHHH
EQINSIQRQSEKPEFANPRNAAGGTLKLLSAKEAAQRNLELSIYGAWAEENTESHYDNLM
HHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCEEEEEEECCCCCCHHHHCCEE
LCQKWGFPVFGRPRQYKSVEEVVKALHEIAKIRDQLPMEIDGVVIKVDNTEDQKVLGMTA
EEHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEECCCCCCHHHCCCH
KHYRWALAYKYAPEQGETILEDILIQVGRTGVLTPVAKLRPVFLSGSKVSRASLYNEEEI
HHHEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCEEEECCCCCHHHHCCCHHHH
ERKDIRIGDTVYVEKGGEIIPKVVGVCLEKRPEGTKPWVMPEYCPVCHERVSRESDKVSV
HHHCEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
RCTNSSCSAGTIEKIRFFVGREALDIEHLGEKVITKLFDLGLIHRRCDIFQITEEDLLQV
EECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHC
PGFKDKSIKNVLKSIEKAKSAPLERFIAALGIPYVGIGGANALAQHFLSLDAVMSASLEE
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
LKVIDGIGSKVAESIVDYFHQSDNVEEIQKMLSLGVNVLPYSRASASCQGKTFVITGSLE
HHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCHH
KMTRSEAEASIRNCGGKVGSSVSKSINYLVVGKDPGSKLKKAQELQIPILNESDLLKILY
HHHHHHHHHHHHHCCCHHCCHHHCCCCEEEEECCCCHHHHHHHHCCCEEECCCCEEEEEC
PN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA