Click here to switch to the map view.

The map label for this gene is gmk

Identifier: 89898165

GI number: 89898165

Start: 420262

End: 420876

Strand: Direct

Name: gmk

Synonym: CF0358

Alternate gene names: 89898165

Gene position: 420262-420876 (Clockwise)

Preceding gene: 89898164

Following gene: 89898166

Centisome position: 36.04

GC content: 38.86

Gene sequence:

>615_bases
ATGAAAGATAAAGTTAGTTTCCCCTTTTCTCCCGATCGACCTCTGTGTGTACCGAAGCTATTTACTATTAGTGCTCCTGC
CGGAGCGGGGAAAACTACGCTAGTGCGTATGTTAGCAGAGGAATTCCCGGATTCTTTTCAAAAAACTGTGTCATTAACAA
CGCGATCGCCTCGTCCTGAAGAAGTTCACGGTGTAGATTATTATTTTGTTTCTCAAGAAGAGTTTCTGAAACGATTAGAT
AGTGGTGATTTTCTAGAATGGGTAGCTCTTTTCGGAGAATATTACGGAACTAGTCGTTTAGAAATTGATAAAATTTGGAA
ATCTGGAAAACACGCGATTGCAGTGATTGATGTGGAGGGAGCTTTAGCTTTACGGAGTAAAATTCCTACCGTGACTATTT
TTATTTCCGCTCCCTCCCTGGAAGAATTAGAAAGGCGTTTAAAACATAGAGGATCTGAACAAGATGCTCAAAGACAAGAA
CGGTTGCAGCACAGTCTCATAGAACAAGCTGCTTCCAGTAAATTTGAATACGTCATTATCAATGATGATTTGGAAAAATC
TTACGAGATTTTGAAAAGTATTTTTATAGCAGAAGAACATAGGAACGTATTATGA

Upstream 100 bases:

>100_bases
ACGGTACTGCTGCTCACTTGGCAGCATTGAAGGCTTTCGGTCCCTGTGATTGTCATAGGAAGAGTTTTGCTCCCATAAGG
CGCAGGTAGTCTGATATTTT

Downstream 100 bases:

>100_bases
CCAACAAAGATCGTTTAACCAATGAAAAGTTAAACCAATTGTTTGAAAGCCCATTTAGTTTGGTTAATTATGCAATCAAA
CAGGCAAAAATCAGGATAGC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKDKVSFPFSPDRPLCVPKLFTISAPAGAGKTTLVRMLAEEFPDSFQKTVSLTTRSPRPEEVHGVDYYFVSQEEFLKRLD
SGDFLEWVALFGEYYGTSRLEIDKIWKSGKHAIAVIDVEGALALRSKIPTVTIFISAPSLEELERRLKHRGSEQDAQRQE
RLQHSLIEQAASSKFEYVIINDDLEKSYEILKSIFIAEEHRNVL

Sequences:

>Translated_204_residues
MKDKVSFPFSPDRPLCVPKLFTISAPAGAGKTTLVRMLAEEFPDSFQKTVSLTTRSPRPEEVHGVDYYFVSQEEFLKRLD
SGDFLEWVALFGEYYGTSRLEIDKIWKSGKHAIAVIDVEGALALRSKIPTVTIFISAPSLEELERRLKHRGSEQDAQRQE
RLQHSLIEQAASSKFEYVIINDDLEKSYEILKSIFIAEEHRNVL
>Mature_204_residues
MKDKVSFPFSPDRPLCVPKLFTISAPAGAGKTTLVRMLAEEFPDSFQKTVSLTTRSPRPEEVHGVDYYFVSQEEFLKRLD
SGDFLEWVALFGEYYGTSRLEIDKIWKSGKHAIAVIDVEGALALRSKIPTVTIFISAPSLEELERRLKHRGSEQDAQRQE
RLQHSLIEQAASSKFEYVIINDDLEKSYEILKSIFIAEEHRNVL

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=179, Percent_Identity=34.6368715083799, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI4504221, Length=179, Percent_Identity=34.6368715083799, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI226874831, Length=179, Percent_Identity=34.6368715083799, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI38570142, Length=170, Percent_Identity=31.1764705882353, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI21361598, Length=188, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI148539578, Length=175, Percent_Identity=32, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI148539628, Length=165, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI14780902, Length=194, Percent_Identity=26.8041237113402, Blast_Score=69, Evalue=2e-12,
Organism=Homo sapiens, GI186700627, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=4e-12,
Organism=Homo sapiens, GI186700629, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI186972120, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI119943106, Length=162, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI4505237, Length=180, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI91199538, Length=165, Percent_Identity=32.7272727272727, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI261862237, Length=162, Percent_Identity=31.4814814814815, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI262118263, Length=180, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI218156338, Length=165, Percent_Identity=32.7272727272727, Blast_Score=64, Evalue=6e-11,
Organism=Homo sapiens, GI262118255, Length=180, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI119943108, Length=162, Percent_Identity=31.4814814814815, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI262118259, Length=180, Percent_Identity=24.4444444444444, Blast_Score=64, Evalue=7e-11,
Organism=Homo sapiens, GI218156340, Length=165, Percent_Identity=32.7272727272727, Blast_Score=64, Evalue=8e-11,
Organism=Homo sapiens, GI218156270, Length=165, Percent_Identity=32.7272727272727, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1790080, Length=185, Percent_Identity=40.5405405405405, Blast_Score=136, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI193203132, Length=179, Percent_Identity=37.9888268156425, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17508835, Length=184, Percent_Identity=37.5, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17557440, Length=184, Percent_Identity=32.6086956521739, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988041, Length=178, Percent_Identity=27.5280898876405, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71988048, Length=178, Percent_Identity=27.5280898876405, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320662, Length=182, Percent_Identity=41.2087912087912, Blast_Score=143, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21356141, Length=185, Percent_Identity=34.5945945945946, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161078485, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24648810, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI161078487, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281362232, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24648808, Length=177, Percent_Identity=27.683615819209, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI281362230, Length=177, Percent_Identity=27.683615819209, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45554804, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24641280, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45554813, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161077752, Length=168, Percent_Identity=30.952380952381, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161077754, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45554791, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281360700, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641282, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45554818, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_CHLFF (Q255A8)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515275.1
- ProteinModelPortal:   Q255A8
- SMR:   Q255A8
- STRING:   Q255A8
- GeneID:   3958569
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0358
- eggNOG:   COG0194
- HOGENOM:   HBG671982
- OMA:   EWAEFAG
- ProtClustDB:   PRK00300
- BioCyc:   CFEL264202:CF0358-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 23295; Mature: 23295

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDKVSFPFSPDRPLCVPKLFTISAPAGAGKTTLVRMLAEEFPDSFQKTVSLTTRSPRPE
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
EVHGVDYYFVSQEEFLKRLDSGDFLEWVALFGEYYGTSRLEIDKIWKSGKHAIAVIDVEG
CCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEHHHHHHCCCCEEEEEECCC
ALALRSKIPTVTIFISAPSLEELERRLKHRGSEQDAQRQERLQHSLIEQAASSKFEYVII
HHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
NDDLEKSYEILKSIFIAEEHRNVL
CCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKDKVSFPFSPDRPLCVPKLFTISAPAGAGKTTLVRMLAEEFPDSFQKTVSLTTRSPRPE
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
EVHGVDYYFVSQEEFLKRLDSGDFLEWVALFGEYYGTSRLEIDKIWKSGKHAIAVIDVEG
CCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEHHHHHHCCCCEEEEEECCC
ALALRSKIPTVTIFISAPSLEELERRLKHRGSEQDAQRQERLQHSLIEQAASSKFEYVII
HHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
NDDLEKSYEILKSIFIAEEHRNVL
CCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA