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The map label for this gene is rnhB [H]

Identifier: 89898164

GI number: 89898164

Start: 419621

End: 420250

Strand: Direct

Name: rnhB [H]

Synonym: CF0357

Alternate gene names: 89898164

Gene position: 419621-420250 (Clockwise)

Preceding gene: 89898163

Following gene: 89898165

Centisome position: 35.98

GC content: 43.33

Gene sequence:

>630_bases
ATGCATACCCTAGTTGAAGAACAGCTGTTTTTGTCTAAGACTATCTTTGAAGAAGAAGTTTTTAGTGAGGGCTTTTCCAT
TATAGCTGGTGTAGATGAGGTCGGTCGAGGCCCATTAGCAGGACCTGTGGTTGCGGGAGCCTGCATTCTTCCTCGAGGAA
AGATATTTGCCGGAGTGAATGATAGCAAGAAACTTTCCCCCAAGGAAAGAGGCAGAATGCGCGATATTCTTGTGAATGAT
CCGGATGTATATTATGGAATCGGAGTTGTCTCTGTAGAAAGAATAGATGAGATCAATATCCTTGAAGCGACCAAGGAAGC
TATGGCAGTAGCTATAGCCAATCTACCTGTTCATCCTGATTTTCTTCTTGTGGATGGTTTGCATCTGCCCCATAAGATTC
CTTGTAAGAAGATTATTAAAGGGGATTCAAAAGTAGCCTCCATTGCTGCAGCATCGATTATAGCTAAAGAATATAGAGAT
GATTTAATGCGAGAGCTTCACCAGCGTTATCCTGATTATGGTTTTGATAAGCATAAGGGTTACGGTACTGCTGCTCACTT
GGCAGCATTGAAGGCTTTCGGTCCCTGTGATTGTCATAGGAAGAGTTTTGCTCCCATAAGGCGCAGGTAG

Upstream 100 bases:

>100_bases
CTGCTAAAGTTAAAGAGTATATTGGTCCTAGATCTTCTAAAAAATAGTTATAGCTATTGAATCTAGCTGATTCCTGATTT
TATTTAAGACATACCGATCT

Downstream 100 bases:

>100_bases
TCTGATATTTTATGAAAGATAAAGTTAGTTTCCCCTTTTCTCCCGATCGACCTCTGTGTGTACCGAAGCTATTTACTATT
AGTGCTCCTGCCGGAGCGGG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MHTLVEEQLFLSKTIFEEEVFSEGFSIIAGVDEVGRGPLAGPVVAGACILPRGKIFAGVNDSKKLSPKERGRMRDILVND
PDVYYGIGVVSVERIDEINILEATKEAMAVAIANLPVHPDFLLVDGLHLPHKIPCKKIIKGDSKVASIAAASIIAKEYRD
DLMRELHQRYPDYGFDKHKGYGTAAHLAALKAFGPCDCHRKSFAPIRRR

Sequences:

>Translated_209_residues
MHTLVEEQLFLSKTIFEEEVFSEGFSIIAGVDEVGRGPLAGPVVAGACILPRGKIFAGVNDSKKLSPKERGRMRDILVND
PDVYYGIGVVSVERIDEINILEATKEAMAVAIANLPVHPDFLLVDGLHLPHKIPCKKIIKGDSKVASIAAASIIAKEYRD
DLMRELHQRYPDYGFDKHKGYGTAAHLAALKAFGPCDCHRKSFAPIRRR
>Mature_209_residues
MHTLVEEQLFLSKTIFEEEVFSEGFSIIAGVDEVGRGPLAGPVVAGACILPRGKIFAGVNDSKKLSPKERGRMRDILVND
PDVYYGIGVVSVERIDEINILEATKEAMAVAIANLPVHPDFLLVDGLHLPHKIPCKKIIKGDSKVASIAAASIIAKEYRD
DLMRELHQRYPDYGFDKHKGYGTAAHLAALKAFGPCDCHRKSFAPIRRR

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=45.6521739130435, Blast_Score=153, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 23019; Mature: 23019

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTLVEEQLFLSKTIFEEEVFSEGFSIIAGVDEVGRGPLAGPVVAGACILPRGKIFAGVN
CCCHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCCCCCCCHHHHCCCCCCCCCEEECCC
DSKKLSPKERGRMRDILVNDPDVYYGIGVVSVERIDEINILEATKEAMAVAIANLPVHPD
CCCCCCHHHHCCHHHEEECCCCEEEEECEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
FLLVDGLHLPHKIPCKKIIKGDSKVASIAAASIIAKEYRDDLMRELHQRYPDYGFDKHKG
EEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
YGTAAHLAALKAFGPCDCHRKSFAPIRRR
CCHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHTLVEEQLFLSKTIFEEEVFSEGFSIIAGVDEVGRGPLAGPVVAGACILPRGKIFAGVN
CCCHHHHHHHHHHHHHHHHHHHCCHHEEECHHHHCCCCCCCHHHHCCCCCCCCCEEECCC
DSKKLSPKERGRMRDILVNDPDVYYGIGVVSVERIDEINILEATKEAMAVAIANLPVHPD
CCCCCCHHHHCCHHHEEECCCCEEEEECEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCC
FLLVDGLHLPHKIPCKKIIKGDSKVASIAAASIIAKEYRDDLMRELHQRYPDYGFDKHKG
EEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
YGTAAHLAALKAFGPCDCHRKSFAPIRRR
CCHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12682364 [H]