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The map label for this gene is murD

Identifier: 89897956

GI number: 89897956

Start: 180741

End: 182000

Strand: Direct

Name: murD

Synonym: CF0149

Alternate gene names: 89897956

Gene position: 180741-182000 (Clockwise)

Preceding gene: 89897955

Following gene: 89897957

Centisome position: 15.5

GC content: 39.92

Gene sequence:

>1260_bases
GTGAATCATCAACGTGTCGTAGTTTTAGGTGCTGGTGTTACAGGAAAATCTGCAGCAGAGTTTCTTCATAAAAAAGGAGA
TTTTGTTATCGGAATTGATGGATCTTGGGATGCTCTTATCTCCTGTAACTTTTTCCACCAACGTTATCTAGACAAAACTG
AAAACTTTCCTGAAGATGTCGATTTGTTCGTGCGTTCTCCGGGGATCAAAACCTCACACCCTCTTGTTATTGAAGCAAAA
CGTCGAGATATTCCTATTGTTACTGACGTACAGCTGGCTTTTCAATCTCCGGAATTTTATCAATATCCCTCTCTTGGAAT
CACAGGTTCCACAGGGAAAACAACAACGGTGCTGTTTTTAGTGCATTTACTGCACTCTTTAGGAATATCTGCTTTTGCTA
TGGGAAATATTGGTTTCCCTATACTTCAAGCAATGTATCAAAAAGGTGTTCGTGTTGTTGAAATCAGTTCGTTTCAATTA
ACTGAACAAGAACAAAAGATCCCCGTATTGTCTGGAGCTGCAATTTTAAATATTTCTGAGAATCATTTAGATTATCATCA
AACTCTACATGCCTATAGTGAGGCAAAAATGAACATTGCCAAATGTTTGCAATGGCCTGATTCTTTATGGTCTGGAGAGG
GAGTTTCTTCTGGAAGGTCTTACCTAGAATATACCGAAGAAATAGATTCGGTTTTAGATAAAGGGGGTGCATTAAAACCA
CTATACTTGCATGATAGGAATAATTATTGCGCAGCCTATGCTTTAGCTAAAGAAGTGACGAGCGTTCCTTTGGAGGCGTT
TTTACAGGCAGTTCAAACTTTTGAAAAACCTCCCCATAGGATAGAATATCTGGGAGAAAAAGATGGCGTGCGTTATATCA
ATGATAGTAAGGCTACCACTATGAGCTCTGTCGAAAAAGCTCTAATGGCTGTAAAAGAAAACGTCATTGTTATTATGGGC
GGGAGGAACAAAGAGAGTAATTTTACCTCTTTAATTCCAGTCCTTACTCAAACGGTAAAACATATTGTGGCTATGGGGGA
GTGTCGGAAAGAGATTGCCCAAGCTTTATCTAGTAGTCTTCCTTTAACTCAAGCCCGAGATCTACAAGAGGCAGTAAGCA
TAGCCCAGAGTATAGCACAGCCGGGTGATGTCATTTTATTATCTCCTGGATGTGCAAGTTTTGACCAGTTTCGAAGCTTT
GAGGAACGAGGGGATTGCTTTAGGCAATTGGTTGGAGATATGGAGGCGTTAAAGATATGA

Upstream 100 bases:

>100_bases
GTATAAAGGCATACCAGAAACTCAGGTTGTCGCACGTTTTTGGATGGCAGGATTACTTTGTACAGTTTTAGGAATAGTCG
CAGCTTTGTGGAGATAGTTT

Downstream 100 bases:

>100_bases
ACCGTAGAGATACGATTATAATCGCAACTTTAGTAAATGTTGTTTTGGTTCTGGTACTCTTTACCACAGCAAAACAGGCA
GATAAGAAAAATGCGGATGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNHQRVVVLGAGVTGKSAAEFLHKKGDFVIGIDGSWDALISCNFFHQRYLDKTENFPEDVDLFVRSPGIKTSHPLVIEAK
RRDIPIVTDVQLAFQSPEFYQYPSLGITGSTGKTTTVLFLVHLLHSLGISAFAMGNIGFPILQAMYQKGVRVVEISSFQL
TEQEQKIPVLSGAAILNISENHLDYHQTLHAYSEAKMNIAKCLQWPDSLWSGEGVSSGRSYLEYTEEIDSVLDKGGALKP
LYLHDRNNYCAAYALAKEVTSVPLEAFLQAVQTFEKPPHRIEYLGEKDGVRYINDSKATTMSSVEKALMAVKENVIVIMG
GRNKESNFTSLIPVLTQTVKHIVAMGECRKEIAQALSSSLPLTQARDLQEAVSIAQSIAQPGDVILLSPGCASFDQFRSF
EERGDCFRQLVGDMEALKI

Sequences:

>Translated_419_residues
MNHQRVVVLGAGVTGKSAAEFLHKKGDFVIGIDGSWDALISCNFFHQRYLDKTENFPEDVDLFVRSPGIKTSHPLVIEAK
RRDIPIVTDVQLAFQSPEFYQYPSLGITGSTGKTTTVLFLVHLLHSLGISAFAMGNIGFPILQAMYQKGVRVVEISSFQL
TEQEQKIPVLSGAAILNISENHLDYHQTLHAYSEAKMNIAKCLQWPDSLWSGEGVSSGRSYLEYTEEIDSVLDKGGALKP
LYLHDRNNYCAAYALAKEVTSVPLEAFLQAVQTFEKPPHRIEYLGEKDGVRYINDSKATTMSSVEKALMAVKENVIVIMG
GRNKESNFTSLIPVLTQTVKHIVAMGECRKEIAQALSSSLPLTQARDLQEAVSIAQSIAQPGDVILLSPGCASFDQFRSF
EERGDCFRQLVGDMEALKI
>Mature_419_residues
MNHQRVVVLGAGVTGKSAAEFLHKKGDFVIGIDGSWDALISCNFFHQRYLDKTENFPEDVDLFVRSPGIKTSHPLVIEAK
RRDIPIVTDVQLAFQSPEFYQYPSLGITGSTGKTTTVLFLVHLLHSLGISAFAMGNIGFPILQAMYQKGVRVVEISSFQL
TEQEQKIPVLSGAAILNISENHLDYHQTLHAYSEAKMNIAKCLQWPDSLWSGEGVSSGRSYLEYTEEIDSVLDKGGALKP
LYLHDRNNYCAAYALAKEVTSVPLEAFLQAVQTFEKPPHRIEYLGEKDGVRYINDSKATTMSSVEKALMAVKENVIVIMG
GRNKESNFTSLIPVLTQTVKHIVAMGECRKEIAQALSSSLPLTQARDLQEAVSIAQSIAQPGDVILLSPGCASFDQFRSF
EERGDCFRQLVGDMEALKI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=440, Percent_Identity=31.1363636363636, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CHLFF (Q255W7)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515066.1
- ProteinModelPortal:   Q255W7
- SMR:   Q255W7
- STRING:   Q255W7
- GeneID:   3958963
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0149
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- ProtClustDB:   PRK00683
- BioCyc:   CFEL264202:CF0149-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46307; Mature: 46307

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQRVVVLGAGVTGKSAAEFLHKKGDFVIGIDGSWDALISCNFFHQRYLDKTENFPEDV
CCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCH
DLFVRSPGIKTSHPLVIEAKRRDIPIVTDVQLAFQSPEFYQYPSLGITGSTGKTTTVLFL
HHEEECCCCCCCCCEEEEECCCCCCEEEEHHHEECCCCCEECCCCCEECCCCCHHHHHHH
VHLLHSLGISAFAMGNIGFPILQAMYQKGVRVVEISSFQLTEQEQKIPVLSGAAILNISE
HHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEECCEEECCHHHCCCEEECCEEEEECH
NHLDYHQTLHAYSEAKMNIAKCLQWPDSLWSGEGVSSGRSYLEYTEEIDSVLDKGGALKP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
LYLHDRNNYCAAYALAKEVTSVPLEAFLQAVQTFEKPPHRIEYLGEKDGVRYINDSKATT
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCHHH
MSSVEKALMAVKENVIVIMGGRNKESNFTSLIPVLTQTVKHIVAMGECRKEIAQALSSSL
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLTQARDLQEAVSIAQSIAQPGDVILLSPGCASFDQFRSFEERGDCFRQLVGDMEALKI
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCC
>Mature Secondary Structure
MNHQRVVVLGAGVTGKSAAEFLHKKGDFVIGIDGSWDALISCNFFHQRYLDKTENFPEDV
CCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCH
DLFVRSPGIKTSHPLVIEAKRRDIPIVTDVQLAFQSPEFYQYPSLGITGSTGKTTTVLFL
HHEEECCCCCCCCCEEEEECCCCCCEEEEHHHEECCCCCEECCCCCEECCCCCHHHHHHH
VHLLHSLGISAFAMGNIGFPILQAMYQKGVRVVEISSFQLTEQEQKIPVLSGAAILNISE
HHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEECCEEECCHHHCCCEEECCEEEEECH
NHLDYHQTLHAYSEAKMNIAKCLQWPDSLWSGEGVSSGRSYLEYTEEIDSVLDKGGALKP
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
LYLHDRNNYCAAYALAKEVTSVPLEAFLQAVQTFEKPPHRIEYLGEKDGVRYINDSKATT
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEECCCCHHH
MSSVEKALMAVKENVIVIMGGRNKESNFTSLIPVLTQTVKHIVAMGECRKEIAQALSSSL
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLTQARDLQEAVSIAQSIAQPGDVILLSPGCASFDQFRSFEERGDCFRQLVGDMEALKI
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA