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The map label for this gene is gspE [H]

Identifier: 89897874

GI number: 89897874

Start: 75506

End: 77002

Strand: Direct

Name: gspE [H]

Synonym: CF0067

Alternate gene names: 89897874

Gene position: 75506-77002 (Clockwise)

Preceding gene: 89897873

Following gene: 89897875

Centisome position: 6.47

GC content: 42.75

Gene sequence:

>1497_bases
ATGATGGGCGGTAAGGCTTTACTATCCCAAGAGCTTCTTAATGCTCTGCCCTATAGCTTTCTAAAAAAGCACTGCTTATT
GCCGTTAGAAGAGCGAGAAGACAGTGTAACCATGGCTTATGCACGGGCTTCCTCGCTTATAGCCAAAGATGAAGTGCAGT
TACTTATAAAGAAGCCTGTGTTTTTTGTTCTCAAAGATGAAGCGGATATCCTACACCGTTTGCAAAAGATCTACTCTAAT
TTAGAGGGTAAAGCCTCTGATATGCTGTTAAGTATGAAAGATGGGAGTGCTTCTCAGACAAATGAAGAAGAAGATCTTTT
AGAAAGCACAGATTCTGTCCCCGTCGTCCGTTTTCTAAATCTTATCTTAAAAGAAGCTATAGAAGAGCGTACTTCGGATA
TTCATTTCGAACCTTTAGAGGACTCTTTGCGTATACGTTATCGTATAGATGGGGTTCTTCACGATCGCCTTTCACCACCT
GCTCATCTGCGCTCTGCGTTAATCACCAGGATTAAAGTTTTAGCAAAGATGGACATTGCGGAACATCGTCTTCCTCAAGA
CGGGAGAATAAAAATCCAAATAGGTGGTCAGGAAATAGATATGCGTGTAAGCACTGTTCCTGTGATCTACGGAGAACGTG
TTGTTCTAAGGATTTTAGATAAGCGTAATGTAATTTTAGATATCTCCGGATTACACATGCCTGAAGATATCGAACAATCA
TTTAAAGAAGTGGTTTCTGTTCCTGAAGGCATATTATTAGTAACTGGACCAACAGGAAGTGGGAAAACTACAACACTCTA
TAGTGTTATTCAGCATCTTTCAGGGCCTTTTGCCAATATTATGACAATAGAAGACCCTCCTGAATACAAATTGCCAGGAA
TTGCCCAGATTGCTGTGAAGCCAAAAATTGGTCTGTCATTTGCTTGTGGTTTAAGGCACCTGCTCCGTCAGGACCCCGAT
GTTCTTATGGTTGGAGAAATCCGGGATCAAGAAACAGCAGAAATTGCCATACAGGCAGCGTTAACGGGGCACCTCGTTGT
TAGTACTCTTCACACTAATGACGCGATTTCTGCGATTCCTCGTCTGTTAGATATGGGGGTAGAGCCTTATCTATTATCAG
CAACAATCATTGGTGTTGTTGCTCAAAGATTGGTTAGGAAAATCTGTCGTCACTGTAAAGAGCAATGCGCAGCAAATATT
CAAGAAAGAGTATTTTTACAGTCTATAGGTCGCGATCCGGATGCGCCGTTGTTTTTTGGGAAGGGATGTTCGCATTGTTT
CCGTTCCGGATATAAGGGGCGTCAGGGGATTTATGAATTCCTGCATCCCGACACGGCTTTGCGTTCGGAGATCGCTATGC
ATAAACCTTATCATGCATTGCGAGAACATGCCGAGCAGAAGGGTTTTCAGCCACTACTACATCACGGTGTTTCTCTAGCT
TTGTCTGGAGAGACAACACTTGCAGAAGTGTTTCGTGTTACGAAACGGTATGACTAG

Upstream 100 bases:

>100_bases
GCACTATGTGCTGGTGAAGAGGCTGCAAAGGCTGCTCATAAAAAATTAGAACTAATTTCCGCTATAGAGCTACCAGCGTC
TCAAGTAGAGGGGCTCGAGT

Downstream 100 bases:

>100_bases
GGAGGGAGGATATGCCACGATATCGTTATACCTACCTCAATGCAAAAGAGCAGCGTAAACAAGACTGGTTAGACGCGATA
CATCTTCAAGAAGCAAAAGA

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MMGGKALLSQELLNALPYSFLKKHCLLPLEEREDSVTMAYARASSLIAKDEVQLLIKKPVFFVLKDEADILHRLQKIYSN
LEGKASDMLLSMKDGSASQTNEEEDLLESTDSVPVVRFLNLILKEAIEERTSDIHFEPLEDSLRIRYRIDGVLHDRLSPP
AHLRSALITRIKVLAKMDIAEHRLPQDGRIKIQIGGQEIDMRVSTVPVIYGERVVLRILDKRNVILDISGLHMPEDIEQS
FKEVVSVPEGILLVTGPTGSGKTTTLYSVIQHLSGPFANIMTIEDPPEYKLPGIAQIAVKPKIGLSFACGLRHLLRQDPD
VLMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRLLDMGVEPYLLSATIIGVVAQRLVRKICRHCKEQCAANI
QERVFLQSIGRDPDAPLFFGKGCSHCFRSGYKGRQGIYEFLHPDTALRSEIAMHKPYHALREHAEQKGFQPLLHHGVSLA
LSGETTLAEVFRVTKRYD

Sequences:

>Translated_498_residues
MMGGKALLSQELLNALPYSFLKKHCLLPLEEREDSVTMAYARASSLIAKDEVQLLIKKPVFFVLKDEADILHRLQKIYSN
LEGKASDMLLSMKDGSASQTNEEEDLLESTDSVPVVRFLNLILKEAIEERTSDIHFEPLEDSLRIRYRIDGVLHDRLSPP
AHLRSALITRIKVLAKMDIAEHRLPQDGRIKIQIGGQEIDMRVSTVPVIYGERVVLRILDKRNVILDISGLHMPEDIEQS
FKEVVSVPEGILLVTGPTGSGKTTTLYSVIQHLSGPFANIMTIEDPPEYKLPGIAQIAVKPKIGLSFACGLRHLLRQDPD
VLMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRLLDMGVEPYLLSATIIGVVAQRLVRKICRHCKEQCAANI
QERVFLQSIGRDPDAPLFFGKGCSHCFRSGYKGRQGIYEFLHPDTALRSEIAMHKPYHALREHAEQKGFQPLLHHGVSLA
LSGETTLAEVFRVTKRYD
>Mature_498_residues
MMGGKALLSQELLNALPYSFLKKHCLLPLEEREDSVTMAYARASSLIAKDEVQLLIKKPVFFVLKDEADILHRLQKIYSN
LEGKASDMLLSMKDGSASQTNEEEDLLESTDSVPVVRFLNLILKEAIEERTSDIHFEPLEDSLRIRYRIDGVLHDRLSPP
AHLRSALITRIKVLAKMDIAEHRLPQDGRIKIQIGGQEIDMRVSTVPVIYGERVVLRILDKRNVILDISGLHMPEDIEQS
FKEVVSVPEGILLVTGPTGSGKTTTLYSVIQHLSGPFANIMTIEDPPEYKLPGIAQIAVKPKIGLSFACGLRHLLRQDPD
VLMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIPRLLDMGVEPYLLSATIIGVVAQRLVRKICRHCKEQCAANI
QERVFLQSIGRDPDAPLFFGKGCSHCFRSGYKGRQGIYEFLHPDTALRSEIAMHKPYHALREHAEQKGFQPLLHHGVSLA
LSGETTLAEVFRVTKRYD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=443, Percent_Identity=45.823927765237, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1786296, Length=404, Percent_Identity=43.3168316831683, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI87082188, Length=126, Percent_Identity=47.6190476190476, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 55628; Mature: 55628

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGGKALLSQELLNALPYSFLKKHCLLPLEEREDSVTMAYARASSLIAKDEVQLLIKKPV
CCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCE
FFVLKDEADILHRLQKIYSNLEGKASDMLLSMKDGSASQTNEEEDLLESTDSVPVVRFLN
EEEEECCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
LILKEAIEERTSDIHFEPLEDSLRIRYRIDGVLHDRLSPPAHLRSALITRIKVLAKMDIA
HHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EHRLPQDGRIKIQIGGQEIDMRVSTVPVIYGERVVLRILDKRNVILDISGLHMPEDIEQS
HHCCCCCCEEEEEECCCEEEEEEEEECEEECCHHHHHHHCCCCEEEEECCCCCCHHHHHH
FKEVVSVPEGILLVTGPTGSGKTTTLYSVIQHLSGPFANIMTIEDPPEYKLPGIAQIAVK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEC
PKIGLSFACGLRHLLRQDPDVLMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIP
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCHHHHHHH
RLLDMGVEPYLLSATIIGVVAQRLVRKICRHCKEQCAANIQERVFLQSIGRDPDAPLFFG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
KGCSHCFRSGYKGRQGIYEFLHPDTALRSEIAMHKPYHALREHAEQKGFQPLLHHGVSLA
CCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCEEE
LSGETTLAEVFRVTKRYD
ECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MMGGKALLSQELLNALPYSFLKKHCLLPLEEREDSVTMAYARASSLIAKDEVQLLIKKPV
CCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCE
FFVLKDEADILHRLQKIYSNLEGKASDMLLSMKDGSASQTNEEEDLLESTDSVPVVRFLN
EEEEECCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
LILKEAIEERTSDIHFEPLEDSLRIRYRIDGVLHDRLSPPAHLRSALITRIKVLAKMDIA
HHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EHRLPQDGRIKIQIGGQEIDMRVSTVPVIYGERVVLRILDKRNVILDISGLHMPEDIEQS
HHCCCCCCEEEEEECCCEEEEEEEEECEEECCHHHHHHHCCCCEEEEECCCCCCHHHHHH
FKEVVSVPEGILLVTGPTGSGKTTTLYSVIQHLSGPFANIMTIEDPPEYKLPGIAQIAVK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEC
PKIGLSFACGLRHLLRQDPDVLMVGEIRDQETAEIAIQAALTGHLVVSTLHTNDAISAIP
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCEEEEEEECCCHHHHHHH
RLLDMGVEPYLLSATIIGVVAQRLVRKICRHCKEQCAANIQERVFLQSIGRDPDAPLFFG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEC
KGCSHCFRSGYKGRQGIYEFLHPDTALRSEIAMHKPYHALREHAEQKGFQPLLHHGVSLA
CCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCEEE
LSGETTLAEVFRVTKRYD
ECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1944223; 12024217 [H]