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The map label for this gene is gspF [H]

Identifier: 89897875

GI number: 89897875

Start: 77014

End: 78189

Strand: Direct

Name: gspF [H]

Synonym: CF0068

Alternate gene names: 89897875

Gene position: 77014-78189 (Clockwise)

Preceding gene: 89897874

Following gene: 89897876

Centisome position: 6.6

GC content: 42.35

Gene sequence:

>1176_bases
ATGCCACGATATCGTTATACCTACCTCAATGCAAAAGAGCAGCGTAAACAAGACTGGTTAGACGCGATACATCTTCAAGA
AGCAAAAGAGAAGCTTGCTCAGCAGGGCGTTCAGCTTTTATCCATTCGCGAGGTAGCACCTCGTCGTGTGCGAGTAAAAA
ATAATGAGTTAATTGTTTTCTCAAAACAGATGCTTCTTCTTCTCCGCTCAGGGCTTCCTTTATACGAAAGCTTGTCCTCA
CTTCGCGATCAATATCAAGGACAAGCAATGTCAGGACTACTGACAGCGTTTATGGAAAACCTGCGCTCTGGGGGAGCGTT
GTCACAGGCAATGGCGGCCTATCCCGATATTTTCGATAATTTTTATCGCAGCGCTATTCTTGCAGGAGAAAGTGTGGGGA
ACCTAGAAGGATGTTTGCAAAACATCATTGTGGTCTTAGAAGAACGTGAGCAAATGTCTAAGAAACTTACGGCAGCTCTT
AGCTATCCGATAGTTTTATTAGTGTTTTCCCTAGCCGTAGTTCTCTTTTTCCTTACAGGAGTGATTCCCTCTTTGAAGGA
AACCTTCGAGAATATGGAGCCCAATACCCTTACCTCTATAGTATTTAGTCTTAGTGATTTTGTCTGTAAGTACAAATATC
TGCTTTTTGGAATTTTAGTTTCGGGCATTACGAGTATAGTCATTACACGACGTAAACTGTTTTGGAAGAAATGGTTTGAG
AAGATCCTTTTTTCGCTTCCCGGGGCGAGGAATTTCTTCATCAAATTAGGATTTAGTCGGTTTTGCTCCGTCGTTTCTGC
AATTCTTCGTGGTGGAGGAACCCTAATAGAAGGACTAGAGTTGGGCTGTGGAGCGGTTCCTTATGACACGCTGCGAGAAG
ATATGAAGCAAATGATTCACGCGGTTGTTGAAGGAAGTTCTTTAAGTAAGGAACTAGCGAAAAGGCCGTGGGTGCCTAAA
TTAGCCTTGGGAATGGTTTCTTTAGGAGAAGAATCCGGAGAGCTTTCTGAAGTGCTGGGTCACGTTGCACATATTTATAA
TGAAGATACCCAAAAAACACTAACCTGGATAACCTCGTGGTGCCAACCGGTTATCCTTGTGTTTCTTGGAGGAATTATAG
GGATTATTATGTTGGCAATACTTATCCCACTAACAAGTAATATTCAAATTTTATAG

Upstream 100 bases:

>100_bases
AGCCACTACTACATCACGGTGTTTCTCTAGCTTTGTCTGGAGAGACAACACTTGCAGAAGTGTTTCGTGTTACGAAACGG
TATGACTAGGGAGGGAGGAT

Downstream 100 bases:

>100_bases
AACACATGGGTCAAAAAATTATGAAAAAACAAAGACGTAAACAATCCATCACACTCATCGAAATGATGGTTGTTATCACA
TTGATCGGTATTGTTGGTGG

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MPRYRYTYLNAKEQRKQDWLDAIHLQEAKEKLAQQGVQLLSIREVAPRRVRVKNNELIVFSKQMLLLLRSGLPLYESLSS
LRDQYQGQAMSGLLTAFMENLRSGGALSQAMAAYPDIFDNFYRSAILAGESVGNLEGCLQNIIVVLEEREQMSKKLTAAL
SYPIVLLVFSLAVVLFFLTGVIPSLKETFENMEPNTLTSIVFSLSDFVCKYKYLLFGILVSGITSIVITRRKLFWKKWFE
KILFSLPGARNFFIKLGFSRFCSVVSAILRGGGTLIEGLELGCGAVPYDTLREDMKQMIHAVVEGSSLSKELAKRPWVPK
LALGMVSLGEESGELSEVLGHVAHIYNEDTQKTLTWITSWCQPVILVFLGGIIGIIMLAILIPLTSNIQIL

Sequences:

>Translated_391_residues
MPRYRYTYLNAKEQRKQDWLDAIHLQEAKEKLAQQGVQLLSIREVAPRRVRVKNNELIVFSKQMLLLLRSGLPLYESLSS
LRDQYQGQAMSGLLTAFMENLRSGGALSQAMAAYPDIFDNFYRSAILAGESVGNLEGCLQNIIVVLEEREQMSKKLTAAL
SYPIVLLVFSLAVVLFFLTGVIPSLKETFENMEPNTLTSIVFSLSDFVCKYKYLLFGILVSGITSIVITRRKLFWKKWFE
KILFSLPGARNFFIKLGFSRFCSVVSAILRGGGTLIEGLELGCGAVPYDTLREDMKQMIHAVVEGSSLSKELAKRPWVPK
LALGMVSLGEESGELSEVLGHVAHIYNEDTQKTLTWITSWCQPVILVFLGGIIGIIMLAILIPLTSNIQIL
>Mature_390_residues
PRYRYTYLNAKEQRKQDWLDAIHLQEAKEKLAQQGVQLLSIREVAPRRVRVKNNELIVFSKQMLLLLRSGLPLYESLSSL
RDQYQGQAMSGLLTAFMENLRSGGALSQAMAAYPDIFDNFYRSAILAGESVGNLEGCLQNIIVVLEEREQMSKKLTAALS
YPIVLLVFSLAVVLFFLTGVIPSLKETFENMEPNTLTSIVFSLSDFVCKYKYLLFGILVSGITSIVITRRKLFWKKWFEK
ILFSLPGARNFFIKLGFSRFCSVVSAILRGGGTLIEGLELGCGAVPYDTLREDMKQMIHAVVEGSSLSKELAKRPWVPKL
ALGMVSLGEESGELSEVLGHVAHIYNEDTQKTLTWITSWCQPVILVFLGGIIGIIMLAILIPLTSNIQIL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=399, Percent_Identity=26.3157894736842, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786295, Length=367, Percent_Identity=26.7029972752044, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43814; Mature: 43683

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYRYTYLNAKEQRKQDWLDAIHLQEAKEKLAQQGVQLLSIREVAPRRVRVKNNELIVF
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEE
SKQMLLLLRSGLPLYESLSSLRDQYQGQAMSGLLTAFMENLRSGGALSQAMAAYPDIFDN
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FYRSAILAGESVGNLEGCLQNIIVVLEEREQMSKKLTAALSYPIVLLVFSLAVVLFFLTG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIPSLKETFENMEPNTLTSIVFSLSDFVCKYKYLLFGILVSGITSIVITRRKLFWKKWFE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KILFSLPGARNFFIKLGFSRFCSVVSAILRGGGTLIEGLELGCGAVPYDTLREDMKQMIH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVVEGSSLSKELAKRPWVPKLALGMVSLGEESGELSEVLGHVAHIYNEDTQKTLTWITSW
HHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
CQPVILVFLGGIIGIIMLAILIPLTSNIQIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEC
>Mature Secondary Structure 
PRYRYTYLNAKEQRKQDWLDAIHLQEAKEKLAQQGVQLLSIREVAPRRVRVKNNELIVF
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEE
SKQMLLLLRSGLPLYESLSSLRDQYQGQAMSGLLTAFMENLRSGGALSQAMAAYPDIFDN
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FYRSAILAGESVGNLEGCLQNIIVVLEEREQMSKKLTAALSYPIVLLVFSLAVVLFFLTG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIPSLKETFENMEPNTLTSIVFSLSDFVCKYKYLLFGILVSGITSIVITRRKLFWKKWFE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KILFSLPGARNFFIKLGFSRFCSVVSAILRGGGTLIEGLELGCGAVPYDTLREDMKQMIH
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
AVVEGSSLSKELAKRPWVPKLALGMVSLGEESGELSEVLGHVAHIYNEDTQKTLTWITSW
HHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
CQPVILVFLGGIIGIIMLAILIPLTSNIQIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]