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The map label for this gene is leuD [H]

Identifier: 89257139

GI number: 89257139

Start: 1820693

End: 1821262

Strand: Reverse

Name: leuD [H]

Synonym: FTL_1888

Alternate gene names: 89257139

Gene position: 1821262-1820693 (Counterclockwise)

Preceding gene: 89257140

Following gene: 89257138

Centisome position: 96.06

GC content: 32.28

Gene sequence:

>570_bases
ATGCAAGCTTTTAAAAAACTAACATCTAGCGCAATTCCTTTATGGCTAAGTGATATTGATACAGATATGATAATCCCTGC
TAACTTCCTAACTCAAACAACAAAAGATGGTTACGGCAAAAGTTTATTTCATAATTTAAAGGAAAAAGATAGTAGTTTTG
TTTTTAATAATCCTGACTATTCTAATTCTGAAATATTGATTGCTGGAGATAATTTTGGCTGTGGTTCATCAAGAGAGCAT
GCTGTTTGGGCGCTAACTCAAGCAGGTATCAAAGTTATAATTGCACCATCATTCTCTGATATATTTTTTAATAATGCTGC
TAAAAATGGCTTATTGTTAATCTCATTAGACAAAGATACTGTCAAAGAATTATGTGATAAGGCAGAAGATCCAAAATTTA
GTATGACTATTGACCTACAAGAGCAAACAGTTAGTGCTGATGGCAGTATTTATAGTTTTGATTATGACCCTTTCCGTAAA
GATTGTCTTATCAGAGGACTTGATGATATGACTTACCTCATTGAGCATCTAGATATAATCAAACAATTTGAACAATCACA
AAGAGGCTAA

Upstream 100 bases:

>100_bases
AAGGAAGTATAACCCATCTTGCTTCACCACAAACAGTTGCTGCTAGTGCTGTTATGGGTAAGATTTGTAGTGTTGATAAA
TTAGATAAGGAGTTATAACA

Downstream 100 bases:

>100_bases
AATGGAAAAAAATATTGCTATATTAGCAGGTGATGGAATCGGACCTGAAGTAATGGAATCAGCATGATGCTATTGCTAAT
CATAAATTTAACTATATAGA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MQAFKKLTSSAIPLWLSDIDTDMIIPANFLTQTTKDGYGKSLFHNLKEKDSSFVFNNPDYSNSEILIAGDNFGCGSSREH
AVWALTQAGIKVIIAPSFSDIFFNNAAKNGLLLISLDKDTVKELCDKAEDPKFSMTIDLQEQTVSADGSIYSFDYDPFRK
DCLIRGLDDMTYLIEHLDIIKQFEQSQRG

Sequences:

>Translated_189_residues
MQAFKKLTSSAIPLWLSDIDTDMIIPANFLTQTTKDGYGKSLFHNLKEKDSSFVFNNPDYSNSEILIAGDNFGCGSSREH
AVWALTQAGIKVIIAPSFSDIFFNNAAKNGLLLISLDKDTVKELCDKAEDPKFSMTIDLQEQTVSADGSIYSFDYDPFRK
DCLIRGLDDMTYLIEHLDIIKQFEQSQRG
>Mature_189_residues
MQAFKKLTSSAIPLWLSDIDTDMIIPANFLTQTTKDGYGKSLFHNLKEKDSSFVFNNPDYSNSEILIAGDNFGCGSSREH
AVWALTQAGIKVIIAPSFSDIFFNNAAKNGLLLISLDKDTVKELCDKAEDPKFSMTIDLQEQTVSADGSIYSFDYDPFRK
DCLIRGLDDMTYLIEHLDIIKQFEQSQRG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=41.2371134020619, Blast_Score=155, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321429, Length=200, Percent_Identity=40, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21212; Mature: 21212

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFKKLTSSAIPLWLSDIDTDMIIPANFLTQTTKDGYGKSLFHNLKEKDSSFVFNNPDY
CCHHHHHHHCCCCEEECCCCCCEEECHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCC
SNSEILIAGDNFGCGSSREHAVWALTQAGIKVIIAPSFSDIFFNNAAKNGLLLISLDKDT
CCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHCCCCCCCEEEEEECHHH
VKELCDKAEDPKFSMTIDLQEQTVSADGSIYSFDYDPFRKDCLIRGLDDMTYLIEHLDII
HHHHHHCCCCCCEEEEEEEHHHEECCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHH
KQFEQSQRG
HHHHHHCCC
>Mature Secondary Structure
MQAFKKLTSSAIPLWLSDIDTDMIIPANFLTQTTKDGYGKSLFHNLKEKDSSFVFNNPDY
CCHHHHHHHCCCCEEECCCCCCEEECHHHHHHCCCCCCHHHHHHHHHHCCCCEEECCCCC
SNSEILIAGDNFGCGSSREHAVWALTQAGIKVIIAPSFSDIFFNNAAKNGLLLISLDKDT
CCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHCCCCCCCEEEEEECHHH
VKELCDKAEDPKFSMTIDLQEQTVSADGSIYSFDYDPFRKDCLIRGLDDMTYLIEHLDII
HHHHHHCCCCCCEEEEEEEHHHEECCCCCEEEECCCHHHHHHHHCCCHHHHHHHHHHHHH
KQFEQSQRG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA